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human teeth germs
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  人牙胚
     Smad1,5 expression and modulation in inner enamel epithelia of human teeth germs cultured in vitro treated by fluoride
     氟化物作用下体外培养人牙胚内釉上皮细胞中Smad1、5表达的变化
短句来源
     METHODS:Human teeth germs were cultured in different densities (10 mg/L and 25 mg/L) of fluoride in vitro with Trowell′s method. To observe the result by immunohistochemical staining.
     方法 :Trowell法体外培养人牙胚 ,用 10mg/L和2 5mg/L的氟分别刺激培养的牙胚 ,免疫组化观察结果。
短句来源
     Methods Human teeth germs were cultured in different densities (10mg/L and 25mg/L) of fluoride in vitro with Trowell's method. The result was observed by immunohistochemical staining.
     方法Trowell法体外培养人牙胚,分别用10mg/L和25mg/L的氟化物刺激培养的牙胚,免疫组化观察结果。
短句来源
     Conclusion Fluoride may inhibit the expression of Smad1, 5 in inner enamel epithelia of human teeth germs cultured in vitro.
     结论氟化物能抑制体外培养的人牙胚内釉上皮细胞中Smad1、5表达。
短句来源
     Objective To investigate Smad1,5 expression and modulation in inner enamel epithelia of human teeth germs cultured in vitro treated by different densities of fluoride and to explore the probable mechanisms inside the cells of dental fluorosis.
     目的观察不同浓度的氟化物对体外培养的人牙胚内釉上皮细胞中Smad1、5表达的影响,从细胞内信号转导水平观察氟对骨形成蛋白(BMP)信号转导的影响。
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  相似匹配句对
     human;
     human ;
短句来源
     THE FOSSIL HUMAN TEETH FROM YUNXIAN,HUBEI
     湖北郧县猿人牙齿化石
短句来源
     Analysis on Trace Elements in Human Teeth
     人齿中微量元素含量的分析探讨
短句来源
     Human Education
     洪恩教育
短句来源
     SWIMMERS TEETH
     游泳者的牙齿
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A cell strain steadily secreting monoclonal antibody against BMP was obtained by hybridoma technique. The monoclonal antibody specifically reacts with the cells of human bone and tooth germs on paraffin embedded tissue sections. Immu-nohistochemical staining shows that BMP is distributed in collagen fibres in normal bone, new osteoid tissue, osteoblasts and the cells of bone icarrow. BMP may also be found in human tooth germs, predentin, the cells of outer and inner enamel epithelium,...

A cell strain steadily secreting monoclonal antibody against BMP was obtained by hybridoma technique. The monoclonal antibody specifically reacts with the cells of human bone and tooth germs on paraffin embedded tissue sections. Immu-nohistochemical staining shows that BMP is distributed in collagen fibres in normal bone, new osteoid tissue, osteoblasts and the cells of bone icarrow. BMP may also be found in human tooth germs, predentin, the cells of outer and inner enamel epithelium, and the cells of dental sac generating alveolar bone. The results prove that antigenic determinants are the same in both the human and bovine BMP. BMP active inhibition test suggests that tins antibody may block the function group of BMP. The ability of the monoclonal antibody to detect antigen and inhibit generation of new bone ma 'kes it poten tially useful in purification of BMP and treatment of os teosarcoma and other bone diseases.

作者用杂交瘤技术获得了纯系细胞株,能稳定分泌抗BMP单克隆抗体。该抗体能与人的骨和牙胚发生反应,具有很高的特异性。免疫组化染色结果表明,BMP存在于骨基质的胶原纤维、新生的骨基质、骨母细胞和骨髓中的未分化间充质细胞中;在人的牙胚中,BMP主要分布在前期牙木质、外釉细胞和造釉细胞以及形成牙槽骨的牙囊细胞中,表明骨的生长和形成以及牙齿的发育都与BMR密切相关。bBMP-McAb能与人的骨和牙胚发生反应,证实人和牛骨的BMP的抗原决定簇是相同的。BMP活性阻断实验证明,新研制的单克隆抗体能有效地封闭BMP活性基因。利用bBMP McAb这一特点,不仅可以纯化BMP,而且可以用来抑制骨的生长,为临床治疗骨肉瘤和其它骨疾病开拓了广阔的前景。

Fibronectin(FN),type Ⅲ collagen,the receptors of concanavalin A and wheat-germ agglutinin were localized in paraffin sections of human tooth germs at different stages of dentinogenesis by immunohistochemistry.FN was present at the border zone between the distal end of odontoblast and predentine,along the odontoblast processes,the cytoplasm of odontoblast or between the odondblasts.FN was enriched in the dental basement membrane at the time of odontoblast differentiation.The spatial distribution and...

Fibronectin(FN),type Ⅲ collagen,the receptors of concanavalin A and wheat-germ agglutinin were localized in paraffin sections of human tooth germs at different stages of dentinogenesis by immunohistochemistry.FN was present at the border zone between the distal end of odontoblast and predentine,along the odontoblast processes,the cytoplasm of odontoblast or between the odondblasts.FN was enriched in the dental basement membrane at the time of odontoblast differentiation.The spatial distribution and temporal cxpression of type Ⅲ collagen,ConA and WGA receptors were also discussed.

本文应用免疫组化方法研究了人牙胚牙乳头细胞分化过程中FN、Ⅲ型胶原、ConA和WGA凝集素受体的分布情况。结果表明:FN阳染于前期牙本质与成牙本质细胞界面、成牙本质细胞突起、细胞顶端胞浆、细胞之间连接处,尤其是在牙乳头间质细胞向成牙本质细胞分化时,内釉基底膜染色增强,同时对Ⅲ型胶原、细胞的ConA、WGA受体的表达变化进行了讨论。

Aim: To prepare a rabbit anti-rat amelogenin IgG antibody and study it's specificity. Methods:complete/imcomplele Freund's adjuvant were used when immunized the rabbit with amelogenin A 1 subcutaneously; The specific IgG antibody was further purified through DE-32 cellulose.The immunological reaction between this antibody and the enamel matrix was determined by immunoelectrophoresis; The histological specificity of this antiserum was tested by immunohistohistochemistry method both in rat incisor...

Aim: To prepare a rabbit anti-rat amelogenin IgG antibody and study it's specificity. Methods:complete/imcomplele Freund's adjuvant were used when immunized the rabbit with amelogenin A 1 subcutaneously; The specific IgG antibody was further purified through DE-32 cellulose.The immunological reaction between this antibody and the enamel matrix was determined by immunoelectrophoresis; The histological specificity of this antiserum was tested by immunohistohistochemistry method both in rat incisor and human tooth germ. Results: The three groups of amelogenin A 1,A 2,A 3 reacted with the anti-A 1 antibody,but had a different shape of precipitin,and the enamelin E1 had no cross reaction with this antiserum. Anti-cytokeratin monoclonal antibody can only react with ameloblast layer, mean while, the anti-amelogenin serum reacted with ameloblast layer having the most positive area on the Tomes processes and the outer enamel layer. Conclusion: The prepared anti-amelogenin A 1 antiserum has a specific immune reaction with the extracellular matrix of ameloblast——amelogenin.

目的:制备抗大鼠釉原蛋白的抗血清,并研究其在组织学上的特异性。方法:采用弗氏完全/不完全佐剂,经皮下多点注射免疫;将所得抗血清用DE-32纤维素纯化其IgG抗体;用微量免疫电泳法测试抗体与釉质基质蛋白之间的免疫反应;用免疫组织化学法测试抗体与大鼠切牙及人牙胚中的组织特异反应。结果:所制备的抗血清与釉原蛋白三种组份A1、A2、A3之间都发生沉淀反应,但沉淀弧形及位置不同,不与釉蛋白E1发生反应。抗角蛋白单克隆抗体与成釉细胞发生强阳性反应,但不与釉质基质发生反应。抗釉原蛋白抗体与成釉细胞发生阳性反应,但在童氏突与釉质浅层区反应最强,不与牙本质细胞发生交叉反应。结论:抗大鼠釉原蛋白抗体能特异地与成釉细胞外基质———釉原蛋白发生免疫反应。

 
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