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quality of dna
相关语句
  dna质量
     Study on Improving the Quantity and the Quality of DNA Extraction from Paraffin-embedded Tissues
     提高石蜡包埋组织中提取DNA质量的实验研究
短句来源
     Immunohistochemical staining for cyclin D1 and semi-nested PCR for t (11;14) were detected in all samples. House-keeping gene β-actin was used to detect the quality of DNA.
     用半巢式聚合酶链反应(PCR)法检测t(11;14 )易位 ,以看家基因 β 肌动蛋白 (actin)作为内对照检测DNA质量
短句来源
     Comparison of DNA Extraction Methods and Effect of Antioxidants on Quality of DNA in Pomegranate
     石榴DNA提取方法的比较及抗氧化剂对DNA质量的影响
短句来源
     The quality of DNA sample is important in RAPD analysis .
     DNA质量是保证RAPD分析成功的关键。
短句来源
     To develop a rapid DNA extraction method,the quality of DNA was examined with soybean dry seeds as materials,adding proteinase K,NP-40 and Tween-20 to SDS extracting solution,and reducing the chloroform/isoamyl extraction times.
     为了探寻大豆干种子中的DNA快速提取方法,以2个大豆品种的干种子为材料,在SDS提取液中加入蛋白酶K、NP-40、Tween-20情况下,并减少氯仿/异戊醇的抽提次数,研究对提取DNA质量的影响。
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  “quality of dna”译为未确定词的双语例句
     The result indicated that the CTAB method,the SDS method,the improved CTAB method may successfully extract DNA,but regardless of the quality and the quantity,the improved CTAB method surpassed conventional CTAB,SDS,DNA extracted mostly presented the colorless transparency by the improved CTAB method,The A260/ A280 values was between 1.65~1.90,This quality of DNA was suitable for RAPD analysis.
     结果表明:CTAB法、SDS法、改良CTAB法均可成功提取树莓DNA,但无论在质量上还是在数量上,改良CTAB法都优于常规CTAB法和SDS法,所提取的DNA大多呈现无色透明状,A260/A280值多数介于1.65~1.90,完全可以用于RAPD分析。
短句来源
     The yield and quality of DNA was analysed by electrophoresis, ratios of A_(260)/A_(280) and PCR amplification.
     通过电泳、紫外吸收A_(260)/A_(280)和PCR扩增检测所得DNA的产量和质量。
短句来源
     The experiments showed that the quality of DNA extracted by this improved method was much higher,DNA stripes were very clear without tagging,the concentration was 1.5397-2.1039μg/L and OD_(260)/OD_(280) was 1.6913-(1.8025).
     该方法具有快速、简易和环境友好的特点。 试验表明,该改良方法所提DNA凝胶条带清晰,无拖尾,浓度在1.5397~2.1039μg/L之间,OD260/OD280检测值在1.6913~1.8025之间,所提DNA适用于PCR,PCR结果理想。
短句来源
     The concentration of alcohol(75%~100%)did not influence the quality of DNA histogram.
     75%~ 1 0 0 %之间的乙醇浓度不影响 DNA直方图的质量 ;
短句来源
     Effect of Preservation Methods for Red Deer Faecal Samples on Extraction Quality of DNA
     马鹿粪便样品保存方法对DNA提取质量的影响
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  相似匹配句对
     The quality
     初步起草了因宁片的质量标准。
短句来源
     Quality.
     质量。
短句来源
     On the Improvement of P. E. Quality
     浅谈体育教学质量的提高
短句来源
     The cost of quality
     质量的价值
短句来源
     On Classification of DNA
     DNA分类方法的探讨
短句来源
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  quality of dna
No differences in either the quantity or the quality of DNA extracted from material dried by these methods was detected.
      
Preparation of DNA from blood stored at room temperature or incubated at 37°C for 24 hr resulted in the same amount and quality of DNA as from samples frozen at -70°C.
      
Preparation of DNA from blood stored at room temperature or incubated at 37°C for 24 hr resulted in the same amount and quality of DNA as from samples frozen at -70°C.
      
13C, 15N labeling of biomolecules allows easier assignments of NMR resonances and provides a larger number of NMR parameters, which greatly improves the quality of DNA structures.
      
Quality of DNA was assessed by optical density measurements and by polymerase chain reaction amplification of a gene coding for the α-4 nicotinic receptor, with the detection of a known polymorphism.
      
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Two temeratures (100℃ and 65℃) and two NaCl concentrations (0. 5mol and 1. 0mol) were compared for DNA Extraction-RAPD Which was modified from Oard's method (Oard J. D. et al, 1992). The genomic DNA electrophoresis and PCR results revealed that the higher quality of DNA was from 65℃-10 min rather than 100℃-10 min extraction, and 1. 0mol NaCl concentration could significantly reduce the production of degraded DNA compared to 0. 5mol NaCl. The consequence is, for some primers, DNA...

Two temeratures (100℃ and 65℃) and two NaCl concentrations (0. 5mol and 1. 0mol) were compared for DNA Extraction-RAPD Which was modified from Oard's method (Oard J. D. et al, 1992). The genomic DNA electrophoresis and PCR results revealed that the higher quality of DNA was from 65℃-10 min rather than 100℃-10 min extraction, and 1. 0mol NaCl concentration could significantly reduce the production of degraded DNA compared to 0. 5mol NaCl. The consequence is, for some primers, DNA from 100℃could diminish the number or attenuate the yields of larger amplification fragments as a result of break point in amplicons.

比较分析了不同温度和NaCl浓度对大豆基因组DNA质量的影响;用DNA聚合酶链式反应(PCR)比较了DNA分离条件不同所导致的PCR扩增产物的差异。100℃、10分钟的提取条件几乎无法得到适于PCR要求的DNA,而65℃、10分钟的抽提条件较易得到合乎要求的DNA。NaCl浓度从0.5mol提高到1.0mol,基本上可以把严重降解的DNA或RNA剔除。65℃,10分钟和1.0molNaCl是新鲜大豆叶片快速分离的理想条件。

To determine the sex of Human bone and tooth,the DYZI and the SRY,the Y specific sequences were amplified by Polymerase Chain Reaction(PCR) with two sets of Primers and DNA isolated from bones and tooth.Results showed that;1 DNA isolation can be finished within 1 hour and 4 hours for bone and tooth respeCtively; 2.The PCR procedures can be achieved within 1.5 hours,3.The quality of DNA from tooth is better than it from bone,4.The sex determination of DNA from boilled bone failed of success...

To determine the sex of Human bone and tooth,the DYZI and the SRY,the Y specific sequences were amplified by Polymerase Chain Reaction(PCR) with two sets of Primers and DNA isolated from bones and tooth.Results showed that;1 DNA isolation can be finished within 1 hour and 4 hours for bone and tooth respeCtively; 2.The PCR procedures can be achieved within 1.5 hours,3.The quality of DNA from tooth is better than it from bone,4.The sex determination of DNA from boilled bone failed of success because of the DNA severe dead-adation.

为了解决刑事案件中利用人骨组织和牙齿进行性别鉴定的问题,我们通过骨骼和牙齿中DNA的提取床用Y染色体特异DNM列引物扩增Y染色体的DYZI和SRY基因序列.结果:1.骨组织中DNA的提取可在1小时内完成;牙齿中DNA的提取可在4小时内完成;2.牙齿中的DNA保存的完好性大于骨组织;4.煮沸过的骨组织中的DNA降解严重,无法用于实验.

The barley cultivar of winter type “Igri”, which was grown in the field, was used as donor plant. The spikes at the mid-uninucleate stage were excised, and then pretreated at 4℃ for 28 days .They were used to study the methods of microspore isolation, dissolution of pollen wall and DNA extraction. The results showed that the yield of isolated microspores was significantly affected by time and speed of microblending. The longer the time of microblending was, the higher the yield of isolated microspores...

The barley cultivar of winter type “Igri”, which was grown in the field, was used as donor plant. The spikes at the mid-uninucleate stage were excised, and then pretreated at 4℃ for 28 days .They were used to study the methods of microspore isolation, dissolution of pollen wall and DNA extraction. The results showed that the yield of isolated microspores was significantly affected by time and speed of microblending. The longer the time of microblending was, the higher the yield of isolated microspores was. The effect of speed of microblending on the yield of isolated microspores was the same as the time. On the other hand, the rate of dissolution of pollen wall was also significantly affected by the different time treatments of ultrasonic appliance. It for 10sec, repeated 6 times and with 10sec interval between repeated treatments was highest in all treatments, which reached 68.85%. However, it for 20sec, repeated 3 times and with 20sec interval between repeated treatments was lowest in all, which reached only 12.77%. Meanwhile, the rate of dissolution of pollen wall was affected by the output control of ultrasonic appliance. The higher the output was, the higher the rate was. We found that the ultrasonic appliance was much better than grinding with liquid N on the rate of dissolution of pollen wall, which was by 11.6 times higher than that of liquid N. Finally, there were not significantly differences on the yield and the quality of DNA extraction between methods of DNA extraction used in this research. They were all very suitable for microspore DNA extraction in barley.

以冬性大麦品种Igri为材料,研究了小孢子游离、破壁及DNA提取方法。结果发现:①提取时间和旋切刀具的转速,对小孢子的得率均有直接影响。时间越长,得率越高。旋切刀具的转速越高,其游离小孢子效果越好,最高为22000rpm,达到94.0。但是当转速在12000rpm以上时,各处理差异不明显;②超声波处理不同时间对小孢子破壁率影响明显,以10s/10s/10s/10s/10s/10s(间隔10s)处理的小孢子破壁率最高达到68.85%;最低的处理为20s/20s/20s(间隔20s),仅为12.77%。前者是后者的5.4倍。此外,超声波的输出功率对小孢子破壁率的作用也十分明显,功率越高,效果越好。超声波法的破壁率明显地优于液氮研磨法。前者是后者的11.6倍;③CTAB法和微量快速提取法2种DNA提取方法的DNA得率和质量无明显差异,均适合于大麦小孢子DNA的提取。

 
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