助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   restriction fragment length differences 的翻译结果: 查询用时:0.186秒
图标索引 在分类学科中查询
所有学科
基础医学
更多类别查询

图标索引 历史查询
 

restriction fragment length differences
相关语句
  限制酶切长度差异
     RESTRICTION FRAGMENT LENGTH DIFFERENCES (FFLDs) OF GENOMIC DNA FROM DIFFERENT GEOGRAPHICALSTRAINS OF ANOPHELES SINENSIS AND ANOPHELES ANTHROPOPHAGUS
     中华按蚊和嗜人按蚊不同地理株基因组DNA的限制酶切长度差异(RFLDs)
短句来源
     RESTRICTION FRAGMENT LENGTH DIFFERENCES OF GENOMIC REPETITIVE DNA FROM FIVE SIBLING SPECIES OF ANOPHELES HYRCANUS GROUP
     赫坎按蚊种团内各亲缘种间基因组重复DNA的限制酶切长度差异
短句来源
     RESTRICTION FRAGMENT LENGTH DIFFERENCES(RFLDS) OF GENOMIC DNA FROM DIFFERENT GEOGRAPHICAL ISOLATES OF ONCOMELANIA HUPENS1S IN MAINLAND CHINA
     中国大陆不同地域隔离群湖北钉螺基因组DNA的限制酶切长度差异
短句来源
     Total DNA was extracted from three geographical strains of Anopheles sinensis and two geographical strains of Anopheles anthropophagus and digested respectively with three restriction endonucleases (Bgl Ⅱ, Hae Ⅲ and Pst Ⅰ). The restriction fragment length differences (RFLDs) of repetitive DNA detected after agarose gel electrophoretic separation and ethidium bromide staining were compared among the above-mentioned geographical strains of both An. sinensis and An.
     我们制备了上海,广西和郑州三株中华按蚊,江苏和广西两株嗜人按蚊的基因组DNA,分别用BglⅡ,HaeⅢ和PstⅠ消化各基因组DNA,进行琼脂糖凝胶电泳再用溴乙锭染色后,观察比较同种按蚊各株间基因组DNA重复序列DNA的限制酶切长度差异.
短句来源
     In order to identify the different strains of Oncamelania hupensis existed in mainland Chain,experiments on extraction of total genomic DNA of different isolates of O. hupensis digested by restriction endonucleases (Bam HI and Pst I) were carried out and restriction fragment length differences (RFLDS) of repetitive DNA detected after agarose gel elec-trophoretic separation were compared among these isolates of snails.
     为证实中国大陆湖北钉螺不同地理株的存在,制备了各地钉螺的基因组DNA,经限制性内切酶Bam HI和Pst I酶解后,置0.8%琼脂糖凝胶电泳,比较分析不同地域隔离群钉螺基因组DNA重复序列DNA的限制酶切长度差异.
短句来源
  “restriction fragment length differences”译为未确定词的双语例句
     Restriction Fragment Length Differences (RFLDs) Analysis of DNA Isolates of Trichinella spiralis from China
     我国4个旋毛虫分离株DNA限制性酶切片断长度差异的比较研究
短句来源
  相似匹配句对
     Restriction Fragment Length Polymorphism in Rice
     水稻DNA限制性片段长度多态性的初步研究
短句来源
     The fragment was indicated specific by restriction enyme analysis.
     将此质粒进行序列分析,结果在两引物间的核酸长度为574bp。
短句来源
     he restriction fragment length polymorphism( RFLP ) of
     采用限制性酶切片段长度多态性分析法,检测在胃癌高发区庄河检出的19例胃癌高系家族成员胃蛋白酶原C基因多态性,发现三种常见片段及一种稀有片段。
短句来源
     Changes of telomeric restriction fragment in colorectal carcinoma.
     大肠癌端粒长度变化的研究
短句来源
     RESTRICTION FRAGMENT LENGTH POLYMORPHISMS(RFLP)IN HUMAN COLORECTAL
     大肠癌的RFLP分析及其p53异质性变化的研究
短句来源
查询“restriction fragment length differences”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  restriction fragment length differences
The mitochondrial DNA of the B10mtJ cells has restriction fragment length differences that allow distinction from the mitochondrial DNA of the NIH/3T3 cells.
      
Since these two strains interbreed, it is possible to regard the restriction fragment length differences (RFLDs) as mutant variants.
      
The linkage mapping of cloned restriction fragment length differences in Caenorabditis elegans
      
Restriction fragment length differences between males and females and between two laboratory stocks of different geographic origin were used to differentiate between sex chromosomal and autosomal origin of the respective fragments.
      
By using a sensitive detection system for small restriction fragment length differences and highly variable potato sequences as probes, all genotypes (diploids and tetraploids) were distinguished by a minimum of two probe/enzyme combinations.
      
更多          


Total DNA was extracted from three geographical strains of Anopheles sinensis and two geographical strains of Anopheles anthropophagus and digested respectively with three restriction endonucleases (Bgl Ⅱ, Hae Ⅲ and Pst Ⅰ). The restriction fragment length differences (RFLDs) of repetitive DNA detected after agarose gel electrophoretic separation and ethidium bromide staining were compared among the above-mentioned geographical strains of both An. sinensis and An. anthropohagus. The results indicated that...

Total DNA was extracted from three geographical strains of Anopheles sinensis and two geographical strains of Anopheles anthropophagus and digested respectively with three restriction endonucleases (Bgl Ⅱ, Hae Ⅲ and Pst Ⅰ). The restriction fragment length differences (RFLDs) of repetitive DNA detected after agarose gel electrophoretic separation and ethidium bromide staining were compared among the above-mentioned geographical strains of both An. sinensis and An. anthropohagus. The results indicated that the band patterns are species- specific and strain- specific, their main bands being similar while their minor bands being distinctly different. Pst Ⅰ digestion produced unique fragments for three geographipal strains of An. sinensis while Bgl Ⅱ digestion produced unique fragments for two geographical strains of An. anthropophagus. In view of the variation in repetitive DNA of different geographical strains of both An. sinensis and An. anthropophagus presented, the RFLDs could be used as a means to distinguish various closely related geographical strains of anopheline mosquitoes.

我们制备了上海,广西和郑州三株中华按蚊,江苏和广西两株嗜人按蚊的基因组DNA,分别用BglⅡ,HaeⅢ和PstⅠ消化各基因组DNA,进行琼脂糖凝胶电泳再用溴乙锭染色后,观察比较同种按蚊各株间基因组DNA重复序列DNA的限制酶切长度差异.结果发现,各地理株间的酶切带型,既具有种的特征,即主带相同,稳定;又有株的特异性淡带。三株中华按蚊的PstⅠ,两株嗜人按蚊的BglⅡ的酶切长度差异可以将各地理株区别开来,比较观察限制酶切长度差异是分析种内各地理株变异的敏感可靠的方法.实验结果表明了中华按故三地理株,嗜人按蚊的两地理株间的重复序列DNA的结构有差异,从基因结构上证实了两种按蚊种下的分化。

Genomic DNA were prepared from 5 sibling species of Anopheles hyrcanus group in-eluding An. sinensis (ASS), An. anthropophagus (ALA), An. liangshanensis (ALS),An: crawfordi (ACW) and An. xiaokuanus (AXK). High molecular weight DNA from each species were cut with three restriction endonuleases (Bgl Ⅱ, Hae Ⅲ and PstⅠ) and the DNA fragments analysed by agarose gel electrophoresis and ethidium bromide st?.inin.g. Three enzymes (Bgl Ⅱ, Hae Ⅲ and PstⅠ) produced unique fragments in all sibling species tested. A diagnostic...

Genomic DNA were prepared from 5 sibling species of Anopheles hyrcanus group in-eluding An. sinensis (ASS), An. anthropophagus (ALA), An. liangshanensis (ALS),An: crawfordi (ACW) and An. xiaokuanus (AXK). High molecular weight DNA from each species were cut with three restriction endonuleases (Bgl Ⅱ, Hae Ⅲ and PstⅠ) and the DNA fragments analysed by agarose gel electrophoresis and ethidium bromide st?.inin.g. Three enzymes (Bgl Ⅱ, Hae Ⅲ and PstⅠ) produced unique fragments in all sibling species tested. A diagnostic restriction fragment length of DNA from 5 sibling species of An. hyrcanus group may be derived from a single restriction enzyme pattern (i. e. Bgl Ⅱ). The results demonstrated that the restriction fragment length differences of repetitive DNA could used as a tool to distinguish sibling species of An. hyrcanus group.

本研究首次报道了赫坎按蚊种团内亲缘种间基因组中重复DNA限制酶切长度差异。研究的蚊种包括中华按蚊、嗜人按蚊、凉山按蚊、克劳按蚊和小宽按蚊等5个亲缘种。制备每种蚊的基因组DNA,分别以BglⅡ、HaeⅡ和PstⅠ等3种限制性核酸内切酶消化,琼脂糖电泳分离,比较重复序列DNA的带型.结果显示5个亲缘种的重复DNA的限制酶切片段的带型有明显的差异.该法敏感可靠,可作为亲缘种间鉴别的一个方法。

The genomic DNA of the two lung flukes (P. westermani and P. skrjabini)from six areas was digested separately by three restriction endonucleases BamHI,Hae Ⅲ and Hind Ⅲ (followed by agarose gel electrophoresis. Repetitive DNA were visusalized as distinct bands in stained gels. Restriction fragment length differences were detected between the two species. The majority of bands of repetitive DNA were similar with minor differences within the same species collected from different areas,upon digestion...

The genomic DNA of the two lung flukes (P. westermani and P. skrjabini)from six areas was digested separately by three restriction endonucleases BamHI,Hae Ⅲ and Hind Ⅲ (followed by agarose gel electrophoresis. Repetitive DNA were visusalized as distinct bands in stained gels. Restriction fragment length differences were detected between the two species. The majority of bands of repetitive DNA were similar with minor differences within the same species collected from different areas,upon digestion with certain restriction endonucleases.The results demonstrated that the technique for demonstrating the band pattern of repetitive DNA with three restriction endonucleases can be used for the identification of P. westermani and P. skrjabini and the geographic relationship of the same species from different areas (Figs. 1 - 3).

从湖南、广东两省六地采集卫氏并殖吸虫和斯氏狸殖吸虫,分别制备各地虫体基因组DNA,用 BamHI、HaeⅢ及 HindⅢ 3种限制性内切酶消化,琼酯糖凝胶电泳后,比较酶切片段长度。结果显示两种虫种间重复 DNA序列带型有明显的差异,而同种虫种不同地区虫体的重复 DNA序列带型则多数相同或完全相同。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关restriction fragment length differences的内容
在知识搜索中查有关restriction fragment length differences的内容
在数字搜索中查有关restriction fragment length differences的内容
在概念知识元中查有关restriction fragment length differences的内容
在学术趋势中查有关restriction fragment length differences的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社