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ordinary pneumonia
相关语句
  普通肺炎
     To investigate the value of detecting Legionella DNA in bronchoalveolar lavage fluids (BALF) in early diagnosis of Legionella pneumonia. The BALF of patients from five groups, including legionella pneumonia group (n=8), clinical suspected legionella pneumonia group (n=15) , ordinary pneumonia group(n=16), pulmonary tuberculosis group (n=15)and lung cancer group (n=15) were examined with DPCR method.
     为探讨采用双重PCR方法 (DPCR)检测支气管肺泡灌洗液 (BALF)标本中军团菌DNA在早期诊断军团菌肺炎方面的意义 ,采用DPCR方法对军团菌肺炎组 (8例 )、临床表现类似军团菌肺炎组 (15例 )、普通肺炎组 (16例 )、肺结核组 (10例 )和肺癌组 (15例 )五组患者留取的BALF标本进行检测。
短句来源
     All of the samples from the ordinary pneumonia group including 40 of sputum and 16 of BALF demonstrated negative DPCR.
     普通肺炎组患者痰和BALF的DPCR结果均为阴性。
短句来源
     To evaluate a Duplex PCR(DPCR) method in early diagnosis of legionella pneumonia by detecting legionella DNA in sputum samples, the sputum samples of patients from four groups, including legionella pneumonia group( n =15), clinical suspected legionella pneumonia group( n =29), ordinary pneumonia group( n =31) and pulmonary tuberculosis group( n =15) were detected with the DPCR method.
     为探讨双重PCR方法 (DPCR)检测痰标本中军团菌DNA在早期诊断军团菌肺炎方面的意义 ,采用DPCR方法对军团菌肺炎组、临床疑似军团菌肺炎组、普通肺炎组和肺结核组 4组患者留取的痰标本进行检测。
短句来源
     The sputa and BALF of patients from three groups,including L.pneumonia group (n=15),clinical suspected L.pneumonia group (n=14) and ordinary pneumonia group(n=25) were collected at early course of disease. All samples were detected with DPCR method for DNA.
     采用DPCR方法对军团菌肺炎组 (15例 )、临床可疑军团菌肺炎组 (14例 )和普通肺炎组 (2 5例 )三组患者在病程早期留取的痰及BALF标本进行检测。
短句来源
     Results All the samples collected from L.pneumonia group,including 25 sputa and 8 BALF showed DPCR positive. The samples of ordinary pneumonia group including 32 sputa and 16 BALF were DPCR negative.
     结果 军团菌肺炎组的所有标本包括 2 5份痰、8份BALF的DPCR结果均为阳性 ,而普通肺炎组的所有标本包括 32份痰和 16份BALF均为DPCR阴性。
短句来源
  相似匹配句对
     Minocycline pneumonia
     米诺环素肺炎
短句来源
     the ordinary were40;
     中40例,占59.7%;
短句来源
     the E ,F ,B are ordinary . and the C. H are bad ;
     E、F、B表现一般,C、H表现不好或较好;
短句来源
     Nosocomial Pneumonia
     医院获得性肺炎
短句来源
     All of the samples from the ordinary pneumonia group including 40 of sputum and 16 of BALF demonstrated negative DPCR.
     普通肺炎组患者痰和BALF的DPCR结果均为阴性。
短句来源
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  ordinary pneumonia
These patients were believed to have delayed resolution of ordinary pneumonia.
      


To investigate the value of detecting Legionella DNA in bronchoalveolar lavage fluids (BALF) in early diagnosis of Legionella pneumonia.The BALF of patients from five groups, including legionella pneumonia group (n=8), clinical suspected legionella pneumonia group (n=15) , ordinary pneumonia group(n=16), pulmonary tuberculosis group (n=15)and lung cancer group (n=15) were examined with DPCR method. The DPCR positive rate of legionella pneumonia group (100%) was significantly higher than...

To investigate the value of detecting Legionella DNA in bronchoalveolar lavage fluids (BALF) in early diagnosis of Legionella pneumonia.The BALF of patients from five groups, including legionella pneumonia group (n=8), clinical suspected legionella pneumonia group (n=15) , ordinary pneumonia group(n=16), pulmonary tuberculosis group (n=15)and lung cancer group (n=15) were examined with DPCR method. The DPCR positive rate of legionella pneumonia group (100%) was significantly higher than those in other four groups (13.33%, 0%, 0%, 0% respectively),( P <0.01). The lowest detection level was 1×10 3 cfu/mL for the simulated BALF sample. The DPCR method adopted for detecting Legionella DNA in BALF samples showed satisfactory sensitivity and specificity.

为探讨采用双重PCR方法 (DPCR)检测支气管肺泡灌洗液 (BALF)标本中军团菌DNA在早期诊断军团菌肺炎方面的意义 ,采用DPCR方法对军团菌肺炎组 (8例 )、临床表现类似军团菌肺炎组 (15例 )、普通肺炎组 (16例 )、肺结核组 (10例 )和肺癌组 (15例 )五组患者留取的BALF标本进行检测。军团菌肺炎组的DPCR阳性检出率为 10 0 % ,明显高于其它四组 (分别为 13 33% ,0 % ,0 % ,0 % ) ,(均P <0 0 1)。模拟BALF标本DPCR的最低检出限为 1× 10 3cfu mL。采用DPCR方法检测BALF标本中的军团菌DNA具有较好的敏感性和特异性 ,此法在临床早期诊断军团菌肺炎方面具有一定的价值

Objective To investigate the value of duplex polymerase chain reaction (DPCR) in early diagnosis of Legionella pneumonia by detecting Legionella DNA in sputum and bronchoalvelar lavage fluid(BALF). Methods During the process of DPCR, two different sets of oligonucleotide primers were stimultaneously used to amplify 386bp 16SrRNA gene fragment and 206bp mip gene fragment. These two primers were designed according to the sequences of 16SrRNA gene and mip gene of Legionella. The sputum...

Objective To investigate the value of duplex polymerase chain reaction (DPCR) in early diagnosis of Legionella pneumonia by detecting Legionella DNA in sputum and bronchoalvelar lavage fluid(BALF). Methods During the process of DPCR, two different sets of oligonucleotide primers were stimultaneously used to amplify 386bp 16SrRNA gene fragment and 206bp mip gene fragment. These two primers were designed according to the sequences of 16SrRNA gene and mip gene of Legionella. The sputum and BALF of patients from two groups, including a Legionella pneumonia group ( n =15) and an ordinary pneumonia group( n =31) were collected at early course of the disease. All of the samples were detected with DPCR for Legionella DNA. Simulated samples were also detected to investigate the sensitivity of the method for testing clinical samples. Results All the samples collected from the Legionella pneumonia patients, including 25 of sputum,and 8 of BALF showed positive DPCR. The results of DPCR, which could distinguish Legionella pneumophila from non pneumophila Legionella spp. to some degree, were in good accordance with those of the specific serum antibodies. All of the samples from the ordinary pneumonia group including 40 of sputum and 16 of BALF demonstrated negative DPCR.Different samples of the same patient showed the same DPCR results. The lowest detection level of simulated sputum sample was the same as that of simulated BALF sample, being 1×10 3 cfu/ml. Conclusions This preliminary study showed that DPCR had satisfactory sensitivity, specificity and stability for detecting Legionella DNA in sputum and BALF. The method is of value in early diagnosis of Legionella pneumonia. Its wide use for clinical work requires further investigation.

目的 探讨双重聚合酶链反应 (DPCR)法检测痰及支气管肺泡灌洗液 (BALF)中军团菌DNA在早期诊断军团菌肺炎的意义。方法 用DPCR对军团菌肺炎组 (15例 )和普通肺炎组 (31例 )患者病程早期留取的痰及BALF进行军团菌DNA检测。用军团菌 16SrRNA基因和mip基因序列引物 ,可扩增 386bp 16SrRNA基因片段和 2 0 6bpmip基因片段。通过模拟标本来检测DPCR方法用于临床标本时的灵敏度。结果 军团菌肺炎组患者痰、BALF的DPCR结果均为阳性 ,其中 13例患者留取的 2 3份痰、7份BALF标本 386bp 16SrRNA基因片段和 2 0 6bpmip基因片段的扩增结果均阳性 ,余 2例患者留取的 2份痰和 1份BALF标本单一 386bp基因片段扩增阳性。DPCR结果与血清军团菌抗体结果相符。普通肺炎组患者痰和BALF的DPCR结果均为阴性。同一患者留取的多份标本呈现相同的DPCR结果。模拟痰和BALF的DPCR最低检出限均为 1× 10 3 cfu/ml。结论 采用DPCR法检测痰和BALF中的军团菌DNA ,具有较好的敏感性、特异性和稳定性 ,在临床早期诊断军团菌...

目的 探讨双重聚合酶链反应 (DPCR)法检测痰及支气管肺泡灌洗液 (BALF)中军团菌DNA在早期诊断军团菌肺炎的意义。方法 用DPCR对军团菌肺炎组 (15例 )和普通肺炎组 (31例 )患者病程早期留取的痰及BALF进行军团菌DNA检测。用军团菌 16SrRNA基因和mip基因序列引物 ,可扩增 386bp 16SrRNA基因片段和 2 0 6bpmip基因片段。通过模拟标本来检测DPCR方法用于临床标本时的灵敏度。结果 军团菌肺炎组患者痰、BALF的DPCR结果均为阳性 ,其中 13例患者留取的 2 3份痰、7份BALF标本 386bp 16SrRNA基因片段和 2 0 6bpmip基因片段的扩增结果均阳性 ,余 2例患者留取的 2份痰和 1份BALF标本单一 386bp基因片段扩增阳性。DPCR结果与血清军团菌抗体结果相符。普通肺炎组患者痰和BALF的DPCR结果均为阴性。同一患者留取的多份标本呈现相同的DPCR结果。模拟痰和BALF的DPCR最低检出限均为 1× 10 3 cfu/ml。结论 采用DPCR法检测痰和BALF中的军团菌DNA ,具有较好的敏感性、特异性和稳定性 ,在临床早期诊断军团菌肺炎方面具有一定的价值

To evaluate a Duplex PCR(DPCR) method in early diagnosis of legionella pneumonia by detecting legionella DNA in sputum samples, the sputum samples of patients from four groups, including legionella pneumonia group( n =15), clinical suspected legionella pneumonia group( n =29), ordinary pneumonia group( n =31) and pulmonary tuberculosis group( n =15) were detected with the DPCR method. Two different sets of oligonucleotide primer were stimultaneously used to amplify 386 bp 16SrRNA gene fragment...

To evaluate a Duplex PCR(DPCR) method in early diagnosis of legionella pneumonia by detecting legionella DNA in sputum samples, the sputum samples of patients from four groups, including legionella pneumonia group( n =15), clinical suspected legionella pneumonia group( n =29), ordinary pneumonia group( n =31) and pulmonary tuberculosis group( n =15) were detected with the DPCR method. Two different sets of oligonucleotide primer were stimultaneously used to amplify 386 bp 16SrRNA gene fragment and 206 bp mip gene fragment of legionella during the DPCR process. The DPCR positive rate of legionella pneumonia group(100%) was significantly higher than that of the other three groups(17.24%, 0, 0, respectively, P <0.01). The lowest detection level was 1×10 3 cfu/mL for the simulated sputum sample. Primary study showed the DPCR method adopted for detecting legionella DNA in sputum samples had satisfactory sensitivity, specificity and stability. The method has its value in early diagnosis of legionella pneumonia.

为探讨双重PCR方法 (DPCR)检测痰标本中军团菌DNA在早期诊断军团菌肺炎方面的意义 ,采用DPCR方法对军团菌肺炎组、临床疑似军团菌肺炎组、普通肺炎组和肺结核组 4组患者留取的痰标本进行检测。DPCR过程采用根据军团菌 1 6SrRNA基因和mip基因设计的 2对引物 ,同时扩增军团菌 3 86bp 1 6SrRNA基因片段和 2 0 6bpmip基因片段。结果 :军团菌肺炎组的DPCR阳性检出率为 1 0 0 % ,明显高于其他 3组 (分别为 1 7.2 4%、0、0 ,P均 <0 .0 1 )。模拟痰标本DPCR的最低检出限为 1× 1 0 3 cfu/mL。初步研究表明 ,DPCR方法检测痰标本中的军团菌DNA具有较好的敏感性、特异性和稳定性 ,此法在临床早期诊断军团菌肺炎方面具有一定的价值

 
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