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dna-binding property
相关语句
  dna结合特性
     Advances in the Studies of Plant Basic Leucine Zipper (bZIP) Proteins (B)——DNA-binding property,gene expression,function and application
     植物碱性亮氨酸拉链(bZIP)蛋白的研究进展(二)——DNA结合特性、基因表达、功能及应用
短句来源
  “dna-binding property”译为未确定词的双语例句
     ORF13 is predicted to be a nuclear protein and a structural protein with probable DNA-binding property. So far, the function of ORF13 is still unknown. Hence, this result provided a theoretical direction for the anti-SARS drug researcher and developer.
     SVMProt将未知功能的ORF13预测为一种可能结合到DNA上的核蛋白并兼有病毒体内结构蛋白质的功能,这一结果对治疗SARS疾病的药物研制工作人员具有一定的理论指导作用。
短句来源
  相似匹配句对
     Conclusions hTR should have specific property for DNA-binding.
     结论人端粒酶具有和端粒DNA片段结合的特性。
短句来源
     EXPRESSION OF HUMAN SRY GENE AND THE DNA BINDING PROPERTY OF ITS PRODUCT
     人SRY基因表达及表达蛋白的结合功能研究
短句来源
     They are DNA binding domains.
     它们的C末端分别含有SAP和SANTDNA结合结构域。
短句来源
     property;
     性质;
短句来源
     Testing the property for DNA-binding of human telomerase by the gel mobility shift assay
     凝胶迁移阻滞法分析人类端粒酶的DNA结合特性
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  dna-binding property
DNA-binding property of recombinant capsid protein of Japanese encephalitis virus
      
Given the localisation and DNA-binding property of the protein, we propose that CpPTP plays a role during the early stages of dehydration-induced chloroplast remodelling.
      


Objective To investigate the role which human SRY gene plays in the regulation of the downstream gene.Methods The fragment of SRY (sex determinatinng region on the Y chromosome) HMG domain was cloned into the expressing vector pET 15b. The hSRY gene recombinant plasmid pETSY was transformed and expressed in E.coliBL21. The target protein was purified by pET His. Tag system. The DNA binding retardation test and its competitive reaction were conducted between SRY protein and the fragment of Müllerian inhibiting...

Objective To investigate the role which human SRY gene plays in the regulation of the downstream gene.Methods The fragment of SRY (sex determinatinng region on the Y chromosome) HMG domain was cloned into the expressing vector pET 15b. The hSRY gene recombinant plasmid pETSY was transformed and expressed in E.coliBL21. The target protein was purified by pET His. Tag system. The DNA binding retardation test and its competitive reaction were conducted between SRY protein and the fragment of Müllerian inhibiting substance (MIS) promoter.Results The molecular weight of the expressed hSRY protein was shown to be approximately 21kD(1kD=0.992U).The specific DNA binding property of SRY protein to the fragment of MIS was confirmed in the retardation test and its competitive reaction.Conclusion The results suggest that the product of SRY gene can bind the MIS promoter region and may initiate the transcription of MIS.

目的研究人类性别决定基因(sexdeterminingregionontheYchromosome,SRY)表达蛋白对下游基因的调节作用。方法将SRY基因HMG结构域片段与表达载体PET-15b-进行重组,构建了SRY基因的表达质粒PETSY,转入大肠杆菌中表达,组氨酸结合树脂柱纯化表达蛋白,与苗勒氏管抑制因子基因(Mülerianinhibitingsubstance,MIS)启动子区片段进行凝胶电泳阻滞试验及竞争试验。结果获得SRY基因表达蛋白,分子量接近20.8U(21kD),凝胶电泳阻滞试验及竞争试验表明,SRY表达蛋白能特异结合位于19号染色体的MIS基因的启动子区。结论提示SRY基因对MIS基因转录具有调节作用。

For investigating the DNA binding property of classical zinc finger protein Zif268,an \%in vivo\% transcription interference experiment was once utilized to develop a genetic selection assay.By screening a library in which the key amino acids of the third zinc finger from Zif268 were randomized,some single fingers with new binding specificity were obtained.In this sutdy,by combining the single fingers,two three-finger peptides cDNA ZF123 and 2ZF123 were constructed by an over-lap PCR technique using the...

For investigating the DNA binding property of classical zinc finger protein Zif268,an \%in vivo\% transcription interference experiment was once utilized to develop a genetic selection assay.By screening a library in which the key amino acids of the third zinc finger from Zif268 were randomized,some single fingers with new binding specificity were obtained.In this sutdy,by combining the single fingers,two three-finger peptides cDNA ZF123 and 2ZF123 were constructed by an over-lap PCR technique using the DNA binding domain of Zif268 as the template.After three times PCR,the products were inserted into pUC18 for cloning.The ZF123 and 2ZF123 cDNA were also inserted into pGEX-2T for expression in \%Escherichia coli\% after sequencing confirmation.The result showed that the three-finger peptides were expressed at a high level in \%E.coli\% JM109.The fusion protein GST-ZF123/2ZF123 have the relative molecular weight of 34 0kD and consisted about 20% of the total soluble cell protein as detected by SDS-PAGE.After supersonic treatment,the soluble part of the bacterial extract was purified.After two additional thrombin cleavage and Sepharose 4B affinity purification steps,the free three-fingers peptide proteins were also obtained.The construction and obtaining of the three-fingers peptide cDNA and its products will facilitate the \%in vivo\% and \%in vitro\% DNA binding specificity study and the design of the hybrid transcription factors.

在获得单一锌指突变体的基础上 ,以小鼠转录因子Zif2 6 8的三锌指DNA结合区为模板 ,利用重叠 (Over lap)PCR技术 ,获得了关键氨基酸位点同时突变的三锌指突变体ZF12 3、2ZF12 3。ZF12 3、2ZF12 3分别克隆进pUC 18质粒 ,序列测定正确后 ,以pGEX 2T为表达质粒 ,在大肠杆菌JM10 9中实现了功能性的表达。经SDS PAGE分析 ,表达出了分子量 34 0kD的融合蛋白 ,扫描分析其含量在 2 0 %左右。菌体经超声波破碎后 ,对可溶性融合蛋白进行了纯化得到了游离的目的蛋白 ,为进一步的DNA结合特性分析、杂交转录因子的构建等奠定了基础。

Identification of the function of SARS coronavirus proteins facilitates the development of therapeutics for treatment of SARS coronavirus infection. We report the identification of the function of some SARS coronavirus proteins by using a Support Vector Machine Program SVMProt. The predicted function of two SARS coronavirus proteins with known function is in agreement with existing knowledge, which indicates the usefulness of SVMProt in functional prediction of SARS coronavirus proteins as well as other proteins....

Identification of the function of SARS coronavirus proteins facilitates the development of therapeutics for treatment of SARS coronavirus infection. We report the identification of the function of some SARS coronavirus proteins by using a Support Vector Machine Program SVMProt. The predicted function of two SARS coronavirus proteins with known function is in agreement with existing knowledge, which indicates the usefulness of SVMProt in functional prediction of SARS coronavirus proteins as well as other proteins. We predict the function of a SARS coronavirus protein with unknown function, ORF13, to be a nuclear protein and a structural protein with probable DNA binding property. 

对SARS冠状病毒蛋白质功能的有效识别将有利于促进SARS传染病治疗药物的开发。应用基于支持向量机原理的SVMProt程序识别SARS冠状病毒蛋白质的功能,通过对SARS冠状病毒中2个已知功能的蛋白质功能的成功预测,说明SVMProt能够有效地应用于SARS冠状病毒蛋白质及其他种类蛋白质的功能预测。对SARS冠状病毒中至今仍未知其功能的蛋白质ORF13的功能进行了预测,结果显示ORF13是一种可能与DNA结合的核蛋白并兼有病毒体内结构蛋白的功能。

 
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