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mouse hepatitis
相关语句
  小鼠肝炎
     EFFECT OF MATRINE ON MOUSE HEPATITIS AND TUMORNECROSIS FACTOR PRODUCTION INDUCED BY PROPIONIBACTERIUM ACNES/ LIPOPOLYSACCHARIDES
     苦参碱对脂多糖/痤疮丙酸杆菌诱导的小鼠肝炎及产生肿瘤坏死因子的影响
短句来源
  “mouse hepatitis”译为未确定词的双语例句
     Mouse hepatitis virus(MHV) is a common coronavirus. The standard strain MHV-A59 was used to infect 6 strains of mice(BALB/c,C57,nu/nu,NIH-Ⅲ,IL-4T and IL-10T) in three paths(belly,oral route and nasal instillation).
     采用MHV-A59标准毒株通过3种途径(腹腔、滴鼻、灌胃)对6个品系的实验小鼠(BALB/c、C57、nu/nu、NIH-Ⅲ、IL-4T、IL-10T)进行了病毒感染。
短句来源
     RT-PCR was performed to check mouse hepatitis virus (MHV) in 84 mice coming from different units, including 6 varieties and 2 grades (conventional and SPF).
     实验应用RT-PCR方法对来自不同单位、6个品系、不同等级的84只小鼠进行了MHV检测。
短句来源
     Four different strains of mouse hepatitis virus (MHV), MHV 1, MHV 3, A 59 and JHM, were used to infect DBT cells. Viral RNA was extracted from the culture liquid.
     用4株小鼠肝炎病毒(MHV1,MHV3,A59,JHM)分别感染DBT细胞,收获病毒,提取病毒RNA。
短句来源
     Results The sera positive rates of Mouse Hepatitis Virus and Sendai Virus were 11% and 19% respectively.
     结果 小鼠肝炎病毒 (MHV)和小鼠仙台病毒 (SV)血清抗体阳性率分别为 11%和 19%。
短句来源
     The antibody variation of laboratory mice inoculated with mouse hepatitis virus
     实验小鼠感染小鼠肝炎病毒后的抗体变化
短句来源
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  相似匹配句对
     ADVANCE IN MOUSE HEPATITIS VIRUS
     小鼠肝炎病毒研究进展
短句来源
     Mouse
     鼠标
短句来源
     STATE OF HEPATITIS B VIRUS IN TRANSGENIC MOUSE
     乙肝病毒(Adr)全基因组在转基因小鼠体内(HBV)DNA的存在状态
短句来源
     THE LION AND THE MOUSE
     狮子和老鼠
短句来源
     Die Hepatitis G
     庚型肝炎
短句来源
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  mouse hepatitis
Here, we generated coronavirus-like particles carrying in their envelope chimeric HCV glycoproteins composed of the ectodomains of E1 and E2, each fused to the transmembrane plus endodomain of the mouse hepatitis coronavirus spike glycoprotein.
      
Three soluble receptor-resistant (srr) mutants, srr7, srr11 and srr18, derived from a highly neurotropic mouse hepatitis virus (MHV) JHMV have a single amino acid mutation in the spike (S) protein.
      
Neurovirulence in mice of soluble receptor-resistant (srr) mutants of mouse hepatitis virus: intensive apoptosis caused by less
      
Mouse hepatitis virus (MHV) utilizes a mouse biliary glycoprotein (Bgp) as a receptor.
      
Difference in Bgp-independent fusion activity among mouse hepatitis viruses
      
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The incidence of mouse colonies with viral infections are common. The most common viral infections are mouse hepatitis, ectromelia, Sendai and Lymphocytic choriomeningitis. latent infections are usually without clinical symptoms. An adequate method is needed to detect these viral infections. We apply the method by measuring the presence of the antibodies in the mouse sera

为检测开放饲养鼠群有无感染鼠肝炎(MHV)鼠痘(Ect.)仙台病毒(Sendai)和淋巴球脉络丛脑膜炎(LCM)等四种病毒性疾病,在我国我们首先应用了ELISA法测定这四种病毒性抗体。检测鼠群来自北京市三个单位送检的开放饲养小鼠,每个单位20~180只不等,采用SPF小鼠的四种病毒抗体的正常OD值是:Sendai为0.125,Ect为0.135,MHV为0.112,LCM为0.130,乘以2.1倍,作为判定血清学阳性的对照,以阻断试验作验证,用免疫粘附血凝试验(IAHA),单扩血溶(SRH)和血凝抑制试验(HI)作比较,在被检的鼠群中检出的阳性率MHV为25.5%,Sendai为20.24%,Ect为2.7%,LCM为3.4%。

Studies on the development of slide enzyme immunoassay (EIA) kits for the detection of antibodies to four viruses (lymphocytic choriomeningitis virus, ectromelia virus, mouse hepatitis virus and Sendai virus) that should be removed from clean laboratory animals was reported.Culture cells infected respectively with these four viruses and uninfected cells were stabilized on the slides by acetone at 2-8℃ as specific and normal antigens. The cross EIA tests indicated that these virus antigens only reacted...

Studies on the development of slide enzyme immunoassay (EIA) kits for the detection of antibodies to four viruses (lymphocytic choriomeningitis virus, ectromelia virus, mouse hepatitis virus and Sendai virus) that should be removed from clean laboratory animals was reported.Culture cells infected respectively with these four viruses and uninfected cells were stabilized on the slides by acetone at 2-8℃ as specific and normal antigens. The cross EIA tests indicated that these virus antigens only reacted with their specific antisera respectively, but did not react with SPF (specific Pathogen free) mouse sera. Compared with HI or ELISA for detecting 112 conventional mouse sera, antibodies were detected to Sendai virus in 19.6% by EIA and in 6.3% by HI. There was prominant difference (P<0.005) . Antibodies were detected to ectromelia virus in 23.3% by EIA and in 21.4% by HI. There was not prominant difference (P>0.05) between them. Antibodies were detected to lymphocytic choriomeningitis virus in 1.8% and to moue hepatitis virus in 71.2% by EIA, and in 1.8% and in 67.6% by ELISA respectively. There was not prominant difference ( P>0.05) between these two methods. 108 mouse sera were detected for antibodies to these four viruses by EIA with two lots of kits, indicating that the repetition rates were between 96-100%. After stored at -18℃ for twelvemonths or 2-8℃ for three months, the EIA antigens of these four viruses were still stable. In a word, the EIA kits for the detection of antibodies to these four viruses were cheap and easily performed as well as sensitive and specific.

本文报道对清洁级实验动物应排除的四种病毒(淋巴细胞脉络丛脑膜炎病毒、小鼠脱脚病病毒、鼠肝炎病毒和仙台病毒)抗体玻片酶免疫(EIA)检测试剂盒的研制。四种病毒感染的细胞和对照细胞经冷丙酮固定于载玻片上制成特异性抗原和对照抗原,此四种病毒的抗血清各10份和SPF小鼠血清20份分别与四种病毒的特异性抗原和对照抗原进行EIA交叉试验,结果显示,抗原只与其相应抗血清发生特异性显色反应,与非特异性小鼠血清和SPF小鼠血清不显色。与HI或ELISA方法比较,通过对112份普通小鼠血清进行测试,结果表明,EIA对仙台病毒抗体的检出率(19.6%)显著高于(<0.005)HI(6.3%),对小鼠脱脚病病毒抗体的检出率(23.3%)与HI(21.4%)无显著性差异(P>0.05)。EIA对淋巴细胞脉络丛脑膜炎病毒和鼠肝炎病毒抗体的检出率分别为1.8%和71.2%,ELISA对两种病毒抗体的检出率分别为1.8%和67.6%,两种方法对两种病毒抗体的检出率无显著性差异(P>0.05)。重复性试验表明两批四种病毒抗体试剂盒对108份小鼠血清两次测定的符合率为96~100%。四种病毒的EIA抗原在-18℃保存12个月或在2-8℃保存3

Biotin carboxyl antiantibody and enzymelabled streptoavidin were used in the indirect enzymelinked immunosorbent assay (ELISA) for the detection of antibodies to mouse hepatitis viruses (MHV) in mice.The results showed that it′s sensitivity was four times higher than that of the ELISA with enzymelabled staphylococcal protein A,and it′s specificity was also better than the latter.

采用生物素-链霉亲和素系统(BSA)试剂建立测定MHV抗体的BSA-ELISA法,并与SPA-ELISA法比较。结果表明,前者本底极低,敏感性比后者提高4倍,阳性检出率提高1倍,且经阻断试验证明其特异性高。因此,该法在检测实验动物的特异性抗体方面,具有很大的应用潜力。

 
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