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retinal extract
相关语句
  视网膜提取物
     Effects of Bovine Retinal Extract on Production of Human IgM in the Culture of Human-Mouse Hybridoma Cells
     牛视网膜提取物对人-鼠杂合细胞抗体产生的影响
短句来源
     Human umbilical vein endothelial cells (HUVEC) were grown for more than 23 passages in medium RPMI-1640 supplemented with 20% calf serum and bovine retinal extract prepared by authors lab.
     本实验用新生小牛视网膜提取物作血管内皮细胞生长促进剂,成功地使人脐静脉内皮细胞在体外连续培养23代以上。
短句来源
     Objective: To study the effects of bovine retinal extract (BRE) on production of human IgM (hIgM) in the culture of human mouse hybridoma cells named G12 . Methods: BRE medium (BREM) was prepared by supplementing 20mg/L protein from BRE to the complete RPMI 1640 medium (CM).
     目的:探讨牛视网膜提取物对人-鼠杂合细胞抗体产生的影响。 方法:在PRMI1640完全培养基(CM)中加入20mg/L牛视网膜提取蛋白,配成牛视网膜培养基(BREM)。
短句来源
  “retinal extract”译为未确定词的双语例句
     ewis rats,aged 6-8 weeks,were given nasally with retinal extract (RE)orS-Ag daily for 10 days prior to immunization with same antigens emulsified in completeFreund’s adjuvant(CFA).
     6一8周龄雌性Lewis大鼠,在用视网膜抽提物(RE)或S-Ag加弗氏完全佐剂(CFA)免疫制成实验性自身免疫性色素膜网膜炎(EAU)动物模型之前鼻腔内给予相同抗原每天1次,共10天。
短句来源
     Methodsmedia of DMEM contained 20% fetal bovine serum(FBS)or added 5% human platelet poor plasma(PPP)and 20 μl/ml retinal extract were used,which were separately combined with separating and weeding for the colonies of cells in primary culture.
     方法 采用含 2 0 %胎牛血清 (FBS)的DMEM和在该培养基中加入 5 %贫血小板人血浆 (PPP)及牛视网膜浸出液 (2 0 μl/ml) ,结合原代培养细胞克隆的分离、除杂。
短句来源
     The soluble antigen was isolated from pig retinal extract by precipitation with ammonium sulfate followed by molecular sieve.
     本实验利用饱和硫酸铵盐析和葡聚糖凝胶过滤层析,从猪视网膜中提取了S抗原。
短句来源
     ConclusionIt can obtain purified endothelial cells and pericytes cultures using media of DMEM contained 20%FBS or adding 5%PPP and 20 μl/ml retinal extract in the media respectively combined with colon separating and weeding of cell in the primary culture
     结论 用 2 0 %FBS的DMEM或在该培养液中加入 5 %PPP及视网膜浸出液 ,结合原代细胞克隆的分离、除杂 ,可分别获得较纯净的PC和EC培养物
短句来源
  相似匹配句对
     WPF Extract
     WPF精粹(一)
短句来源
     The Extract of Inulin
     旋复花素的提取
短句来源
     Retinal Detachment
     视网膜脱离
短句来源
     Retinal Vasculitis
     视网膜血管炎
短句来源
     Effect of hirudinoid extract on retinal pigment epithelium for proliferation induced by thrombin
     水蛭提取液对凝血酶诱导的视网膜色素上皮细胞增殖的影响
短句来源
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  retinal extract
Experimental autoimmune uveoretinitis (EAU), a mostly T-cell dependent disease, was induced in laboratory animals by a single immunization with retinal extract or purified S-antigen in complete Freund's adjuvant.
      
Once isolated, HCC-EC do not require conditioned medium but do require either retinal extract or acidic fibroblast growth factor for survival and growth.
      
An exploitable temporal separation between the emergence of EC and non-EC is obtained when both conditioned medium (from bovine aortic endothelium) and retinal extract are present during the outgrowth period.
      
The optimal culture medium for these cells was 15% equine plasma derived serum, 20% conditioned medium, 2% retinal extract, 60% fresh medium, and 500 μg/ml heparin.
      
The cells were grown in Chee's essential replacement medium containing 1% bovine retinal extract.
      
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The soluble antigen was isolated from pig retinal extract by precipitation with ammonium sulfate followed by molecular sieve. 50 μg S antigen with equal volume complete Freund's adjuvant was injected into double hind foodpads of guinea pigs. Two weeks later, experimental autoimmune uveitis developed. Its pathologic feature is that a great number of lymphocytes infiltrated into chroid which was thickened gradually from anterior to posterior. Inflammatory variation was relative to disease phase. Ultrastructure...

The soluble antigen was isolated from pig retinal extract by precipitation with ammonium sulfate followed by molecular sieve. 50 μg S antigen with equal volume complete Freund's adjuvant was injected into double hind foodpads of guinea pigs. Two weeks later, experimental autoimmune uveitis developed. Its pathologic feature is that a great number of lymphocytes infiltrated into chroid which was thickened gradually from anterior to posterior. Inflammatory variation was relative to disease phase. Ultrastructure pathology was characterized by swelling and necrosis in the inner and outer segments of photorecepter cells. This result suggests that S antigen has significant effect on inducing experimental autoimmune uveitis and the outer layer of retina is the sensitive target organ of S antigen.

本实验利用饱和硫酸铵盐析和葡聚糖凝胶过滤层析,从猪视网膜中提取了S抗原。50 μg S抗原伴等量完全佛氏佐剂,注射于豚鼠后足掌肉垫皮下。两周后,诱发了中度至严重的葡萄膜视网膜炎。其病理特征是:脉络膜内有大量淋巴细胞浸润;由前向后,逐渐增厚。炎症变化与病程有关。超微结构特征性改变是视网膜感光细胞内、外节水肿,变性,坏死。提示:S抗原有强烈诱发自身免疫性葡萄膜视网膜炎的作用;外层网膜是较敏感的靶器官。

Human umbilical vein endothelial cells (HUVEC) were grown for more than 23 passages in medium RPMI-1640 supplemented with 20% calf serum and bovine retinal extract prepared by authors lab. The cells exhibited population doubling time by an average of 16.6 hours. Serial subcultivations were made with an average of 5 days and split ratio of 1:2~3. Immuno-histochemical and transmission electron microscopic examination of the cells showed that the human endothelial cells contained F Ⅷ r-Ag and Weible-Palade's...

Human umbilical vein endothelial cells (HUVEC) were grown for more than 23 passages in medium RPMI-1640 supplemented with 20% calf serum and bovine retinal extract prepared by authors lab. The cells exhibited population doubling time by an average of 16.6 hours. Serial subcultivations were made with an average of 5 days and split ratio of 1:2~3. Immuno-histochemical and transmission electron microscopic examination of the cells showed that the human endothelial cells contained F Ⅷ r-Ag and Weible-Palade's (WP) bodies.

本实验用新生小牛视网膜提取物作血管内皮细胞生长促进剂,成功地使人脐静脉内皮细胞在体外连续培养23代以上。细胞倍增时间平均为16.6h,细胞传代时间平均为5 d,传代比率为1:2~1:3。用ABC法证明细胞内存在Ⅷ因子相关抗原(FⅧr-Ag),透射电镜下可见胞浆内含WP(Weibe1-Palade)小体,证实所培养的细胞为血管内皮细胞。

ewis rats,aged 6-8 weeks,were given nasally with retinal extract (RE)orS-Ag daily for 10 days prior to immunization with same antigens emulsified in completeFreund’s adjuvant(CFA).The results sho wed that both the severity and the incidenee of theclinical responses of experimental autoimmune uveoretinitis(EAU)were effectively sup-pressed. Meanwhile, the suppression of EAU was consistent with the inhibition of RE orS-Ag reactive interferon-γsecreting cells in some lymphoid organs and with the decreaseof...

ewis rats,aged 6-8 weeks,were given nasally with retinal extract (RE)orS-Ag daily for 10 days prior to immunization with same antigens emulsified in completeFreund’s adjuvant(CFA).The results sho wed that both the severity and the incidenee of theclinical responses of experimental autoimmune uveoretinitis(EAU)were effectively sup-pressed. Meanwhile, the suppression of EAU was consistent with the inhibition of RE orS-Ag reactive interferon-γsecreting cells in some lymphoid organs and with the decreaseof skin delayed-type hypersensitivity responses to RE. But rats which were tolerized withRE or S-Ag exhibited a similar antibody response when compared to controls(phosphate-buffered saline given nasally).These results demonstrated that nasal inductionof tolerance suppressed the onset and severity of EAU by inhibiting the pathogenic Tlymphocytes.

6一8周龄雌性Lewis大鼠,在用视网膜抽提物(RE)或S-Ag加弗氏完全佐剂(CFA)免疫制成实验性自身免疫性色素膜网膜炎(EAU)动物模型之前鼻腔内给予相同抗原每天1次,共10天。结果表明:①该耐受疗法可有效地抑制临床疾病的发生率和严重程度;②耐受组疾病的抑制与不同淋巴器官中γ干扰素(IFN-γ)分泌细胞数的减少和RE特异性皮肤迟发性过敏反应(DTH)的抑制相一致;③病程中血清抗体的变化与对照组差异无显著性。提示鼻腔给予RE或S-Ag抑制EAU与其诱导的致病性T淋巴细胞的抑制有关。

 
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