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glycoprotein gene of rabies virus
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  “glycoprotein gene of rabies virus”译为未确定词的双语例句
     Molecular Difference of Nucleotide Sequence of Main Regions of Glycoprotein Gene of Rabies Virus in China
     我国RV野毒株G基因主要功能区核酸序列的分子差异
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     Immune response of mice to replication-defective recombinant adenovirus containing glycoprotein gene of rabies virus 3aG strain
     小鼠对表达狂犬病毒3aG株糖蛋白的重组复制缺陷型腺病毒的免疫应答
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     Methods The full-length glycoprotein gene of rabies virus CVS-N2c was inserted into plasmid pcDNA3.1. COS-7 cells were transfected with the recombinant plasmid pcDNA3.1/CVS-N2c GP and the expression of GP was determined by indirect immune fluorescent test.
     方法 构建狂犬病毒糖蛋白 (GP)的真核表达质粒pcDNA3.1 CVS N2cGP作为核酸疫苗 ,瞬时转染COS 7细胞 ,间接免疫荧光试验检测pcDNA3.1 CVS N2cGP的表达 ;
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     Methods The fowlpox virus (FPV) transfer vector pUTA-RgC was constructed by inserting the glycoprotein gene of rabies virus CTN-181 strain into the downstream of the hybrid promoter ATI·P7.5 of the FPV expressing vector pUTA-2. The chicken embryo fibroblast (CEF) cells were infected with FPV Chinese vaccine strain 282E4 for 2-3 h.
     方法 以中国鸡痘病毒疫苗株 2 82E4为基础构建的表达载体pUTA 2的ATI·P7.5复合启动子下游 ,插入狂犬病毒CTN 181株糖蛋白基因 ,构建了重组转移载体pUTA RgC。
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     Analyzing and Sequencing of the Glycoprotein Gene of Rabies Virus Isolates from Guangxi
     广西12株狂犬病野毒株的g基因序列测定与分析
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     The gene of the glycoprotein g13 of EHV- I was sequenced.
     本文分析了其膜糖蛋白g13基因的碱基序列,并由此推导其相应的氨基酸序列。
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     Expression of Rabies Virus Glycoprotein Gene in E. coli
     狂犬病病毒糖蛋白基因在原核细胞中的表达
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     Analysis of Glycoprotein gene of H9N2 AIV
     H9N2亚型禽流感病毒纤突蛋白基因的分析
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     Cloning, Expression and Purification of the Gene of Glycoprotein G of HSV-2
     单纯疱疹病毒Ⅱ型糖蛋白G基因的克隆、表达及纯化
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     Experimental hepatitis induced by P-glycoprotein gene vaccine in mice
     P-糖蛋白基因疫苗诱导小鼠实验性肝炎
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  glycoprotein gene of rabies virus
Nucleotide and deduced amino acid sequences of the glycoprotein gene of rabies virus vaccine strain Vnukovo-32
      


AIM To prepare expression and DNA immunization by constructing recombinomt plasmid pCI neorabG containing glycoprotein gene of rabies virus 3aG strain. METHODS Total RNA were extracted from rabies virus 3aG infected mouse cerebral tissues, then glycoprotein gene fragment was amplified by RT-PCR and cloned into pUC19 plasmid (named pUC19rabG). The glycoprotein gene was then subcloned into eukaryotic expression vector pCI-neo. RESULTS Specific glycoprotein gene...

AIM To prepare expression and DNA immunization by constructing recombinomt plasmid pCI neorabG containing glycoprotein gene of rabies virus 3aG strain. METHODS Total RNA were extracted from rabies virus 3aG infected mouse cerebral tissues, then glycoprotein gene fragment was amplified by RT-PCR and cloned into pUC19 plasmid (named pUC19rabG). The glycoprotein gene was then subcloned into eukaryotic expression vector pCI-neo. RESULTS Specific glycoprotein gene of rabies virus 3aG strain was successfully amplified and cloned into pUC19rabG. Fur-thermore, a recombinant plasmid pCI-neorabG for eukaryotic expression was also obtained after subcloning, selection and confirmation. CONCLUSION The obtaining of pUC19rabG and pCI-neorabG plasmids has laid a solid foundation for expression and DNA immunization.

目的 构建含狂犬病毒 3a G株糖蛋白的重组质粒p CI- neorab G,为进一步表达及 DNA免疫做准备 .方法 从感染了狂犬病毒 3a G株的鼠脑组织中提取总 RNA进行 RT-PCR,扩增出编码糖蛋白的基因片段 ,重组入 p UC19克隆载体 .再将 p UC19rab G中的糖蛋白基因片段亚克隆入 p CI- neo真核表达载体 .结果 从 3a G株基因组中扩增出特异的糖蛋白基因片段 ,克隆成功 p UC19rab G;经亚克隆 ,筛选鉴定获得了 p CI- neorab G 重组质粒 .结论 构建成功狂犬病毒p U C19rab G重组质粒 ,亚克隆成功 p CI- neorab G重组质粒 ,为下一步表达及 DNA免疫奠定了基础

Objective To investigate the immune response of mice primed by DNA particle bombing and boosted by immunization with replication-defective adenoviral recombinant bearing rabies glycoprotein gene. Methods The full-length glycoprotein gene of rabies virus CVS-N2c was inserted into plasmid pcDNA3.1. COS-7 cells were transfected with the recombinant plasmid pcDNA3.1/CVS-N2c GP and the expression of GP was determined by indirect immune fluorescent test. Mice were immunized with a heterogeneous prime-booster...

Objective To investigate the immune response of mice primed by DNA particle bombing and boosted by immunization with replication-defective adenoviral recombinant bearing rabies glycoprotein gene. Methods The full-length glycoprotein gene of rabies virus CVS-N2c was inserted into plasmid pcDNA3.1. COS-7 cells were transfected with the recombinant plasmid pcDNA3.1/CVS-N2c GP and the expression of GP was determined by indirect immune fluorescent test. Mice were immunized with a heterogeneous prime-booster regime: First, DNA particle bombing vaccination was conducted twice by helium gas drived gene gun at a interval of two weeks. Then, replication defective adenoviral recombinant was used for booster at fifteenth weeks. The level of rabies virus specific antibody was measured by ELISA and the neutralizing antibody was titrated by a rapid fluorescent focus inhibition test (RFFIT). Results Indirect immune fluorescent test indicated that the glycoprotein was successfully expressed on the surface of COS-7 cells. Low level titre of specific antibody was induced by DNA immunization. RFFIT results showed that the neutralizing antibody reached 0.66 IU/ml after secondary DNA immunization and dramatic rising of neutralizing antibody was observed when the mice was boosted with 2×10 6 PFU recombinant replication defective adenovirus, the mean titre reached 90 IU/ml at seventeenth weeks. Conclusion Combined immunization of DNA vaccine and replication-defective recombinant adenovirus bearing rabies CVS-N2c glycoprotein gene induced potential immune response against rabies virus.

目的 研究联合运用狂犬病毒糖蛋白基因重组腺病毒与同一抗原核酸疫苗对小鼠的免疫效果。方法 构建狂犬病毒糖蛋白 (GP)的真核表达质粒pcDNA3.1 CVS N2cGP作为核酸疫苗 ,瞬时转染COS 7细胞 ,间接免疫荧光试验检测pcDNA3.1 CVS N2cGP的表达 ;以基因枪方法进行初次免疫接种和首次加强免疫 ,以表达同一抗原的复制缺陷型重组腺病毒通过鼻腔接种进行第二次加强免疫 ;ELISA试验检测血清狂犬病毒特异性IgG抗体 ,快速荧光灶抑制试验 (RFFIT)检测狂犬病毒中和抗体。结果 间接免疫荧光试验表明pcDNA3.1 CVS N2cGP能有效地表达GP于转染的细胞膜上 ,ELISA试验表明小鼠接受基因枪核酸免疫后 ,仅诱导产生低水平的特异性抗体 ,而用重组腺病毒进行加强免疫后 ,特异性抗体水平显著提高。结论 联合运用狂犬病毒糖蛋白基因重组腺病毒与同种抗原核酸疫苗可克服它们单独使用时各自的缺点 ,能有效地诱导小鼠产生抗狂犬病毒特异免疫。

A plasmid pWS 4 expressed vaccinia virus of glycoprotein gene of vaccinia rabies virus was recombined homologically with wild type of vaccinia virus Tiantan strain (vaccinia virus). After purified by plague, recombinant vaccinia virus (recombinant virus) expressing glycoprotein gene of rabies virus was obtained. And its DNA dot blot showed strong positive signal, cell membrane and cell plasma in indirect immunofluorescence testing also showed strong positive fluorescent reaction. Western blot analysis...

A plasmid pWS 4 expressed vaccinia virus of glycoprotein gene of vaccinia rabies virus was recombined homologically with wild type of vaccinia virus Tiantan strain (vaccinia virus). After purified by plague, recombinant vaccinia virus (recombinant virus) expressing glycoprotein gene of rabies virus was obtained. And its DNA dot blot showed strong positive signal, cell membrane and cell plasma in indirect immunofluorescence testing also showed strong positive fluorescent reaction. Western blot analysis showed a band of non fusion glycoprotein of rabies virus only at 64ku. Titer of induced neutralized antibodies in mice and dogs against rabies reached 2430 and >696 in the 28th day. Protection rate reached up to 80% 14 days after initially immunized with 50~100LD\-\{50\} rabies virus CVS strain into mice from intracerebral challenge. And its pathogenicity was remarkably reduced. Virus purity, virus titer, hemagglutination titer, protein expression, antibody activity and protectiveness showed no differences after successive transfer from 11th generation through 30th generation, indicated that the recombinant virus has good genetic stability.\;

狂犬病毒糖蛋白基因的痘苗病毒表达质粒 pWS 4在鸡胚细胞内与野生型痘苗病毒天坛株 (痘苗病毒 )同源重组 ,经蚀斑纯化 ,获得表达狂犬病毒糖蛋白基因的重组痘苗病毒 (重组病毒 )。该重组病毒DNAdotblot显示强阳性信号 ,间接免疫荧光试验胞膜及胞浆均见到强阳性荧光反应 ,Westernblot分析仅在 6 4ku处呈现一条狂犬病毒非融合性糖蛋白带。免疫小鼠和狗 ,第 2 8d抗狂犬病毒中和抗体滴度分别达 2 4 30和 >6 96。初免后 14d用 5 0~ 10 0LD50 狂犬病毒CVS株对小鼠脑内攻击 ,保护率达 80 %以上。致病性明显减弱。从第 11代起连续传至 30代 ,病毒纯度、病毒滴度、血凝滴度、蛋白的表达、抗原活性和保护性均无差异 ,说明该重组病毒有良好的遗传稳定性。

 
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