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pig retinal
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  猪视网膜
     Protection of zinc against pig retinal ganglion cells' damage induced by microwave
     锌对微波致猪视网膜神经节细胞损伤的防护作用
短句来源
     To determine the morphological variation in the primary cultured pig retinal ganglion cells induced by microwave and the protection of VE can supply some experiment foundation for study of effect of microwave and its protection.
     为探讨微波对体外培养的猪视网膜神经节细胞形态的影响及维生素 E(VE)的防护作用 ,为微波致视网膜损伤的研究提供一定的实验依据。
短句来源
     AIM To determine the lipid peroxidation damage in the primary cultured pig retinal ganglion cells induced by microwave and cells' tolerance to microwave in order to provide some experiment foundation for study of mechanism of microwave damage and its protection.
     目的 探讨微波对体外培养的猪视网膜神经节细胞的脂质过氧化损伤作用及耐受剂量 ,为进一步研究微波的眼底损伤机制及其防护提供一定的实验依据 .
短句来源
     AIM To investigate the protective effect of zincon the microwave induced lipid peroxide damage to in vitro cultured pig retinal ganglion cells and the cells' tolerance to microwave.
     目的 以锌作为防护因素研究微波对体外培养的猪视网膜神经节细胞的脂质过氧化损伤作用及耐受剂量 .
短句来源
     Pig retinal ganglion cells in culture
     猪视网膜神经节细胞的培养及超微结构
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  “pig retinal”译为未确定词的双语例句
     Laser induced Intracellular Free Calcium Changes in Cultured Pig Retinal Pigment Epithelial Cells
     激光损伤致猪视网膜色素上皮细胞中游离钙变化的实验研究
短句来源
     Zinc protective effects on pig retinal pigment epithelial cell damage of lipid peroxide induced by 2450MHz microwave
     2450MHz微波诱导猪视网膜色素上皮细胞脂质过氧化损伤及锌的防护效应
短句来源
     Culture of pig retinal pigment epithelial cell in vitro
     猪视网膜色素上皮细胞的体外培养
短句来源
     To observe the lipid peroxide damage in the cultured pig retinal pigment epithelial cell(RPE) induced by microwave and the protective effects by zinc (Zn). RPE cells were divided into 3 groups in terms of microwave intensity(the group of 10, 20 and 30mW·cm -2 )and each group was exposed to microwave for 1 hour.
     为观察微波对体外培养的猪视网膜色素上皮细胞的脂质过氧化损伤作用及锌的生物防护作用 ,体外培养猪视网膜色素上皮细胞 ,微波按强度分为 3组 (10、2 0、30 m W· cm- 2 )辐照 1h。
短句来源
     AIM:To determine the protection of zinc on lipid peroxidation damage in the primary cultured pig retinal ganglion cells induced by microwave can provide some experiment foundation for study of mechanism of microwave damage and its protection.
     目的 :探讨微量元素锌对微波致视网膜神经节细胞的脂质过氧化损伤的防护作用 ,为进一步研究微波损伤机理及其防护提供一定的实验依据。
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  相似匹配句对
     Pig retinal ganglion cells in culture
     猪视网膜神经节细胞的培养及超微结构
短句来源
     Quantitative study of normal retinal structure in guinea pig
     豚鼠视网膜正常结构的定量研究
短句来源
     Pig's CleaningTechnology
     清管器(Pig)清洗技术
短句来源
     Retinal Detachment
     视网膜脱离
短句来源
     The Year of The Pig
     丁亥猪福
短句来源
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  pig retinal
We have examined the transport of the corresponding antigens of four such MAbs in guinea pig retinal ganglion-cell axons.
      
Glutamate receptor function in vitro was assessed in pig retinal cell cultures by patch clamp recording.
      
The effect of the glial toxin α-aminoadipic acid (AAA) upon theNa+/glutamate cotransporter of acutely isolated guinea pig retinal glial cells was studied using the whole-cell voltage-clamp technique.
      
Density-dependent growth regulation of pig retinal pigment epithelial cells in vitro
      
The possible occurrence of cannabinoid (CB) receptors was studied on superfused guinea-pig retinal discs preincubated with [3H]dopamine or [3H]noradrenaline.
      
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The soluble antigen was isolated from pig retinal extract by precipitation with ammonium sulfate followed by molecular sieve. 50 μg S antigen with equal volume complete Freund's adjuvant was injected into double hind foodpads of guinea pigs. Two weeks later, experimental autoimmune uveitis developed. Its pathologic feature is that a great number of lymphocytes infiltrated into chroid which was thickened gradually from anterior to posterior. Inflammatory variation was relative to disease phase. Ultrastructure...

The soluble antigen was isolated from pig retinal extract by precipitation with ammonium sulfate followed by molecular sieve. 50 μg S antigen with equal volume complete Freund's adjuvant was injected into double hind foodpads of guinea pigs. Two weeks later, experimental autoimmune uveitis developed. Its pathologic feature is that a great number of lymphocytes infiltrated into chroid which was thickened gradually from anterior to posterior. Inflammatory variation was relative to disease phase. Ultrastructure pathology was characterized by swelling and necrosis in the inner and outer segments of photorecepter cells. This result suggests that S antigen has significant effect on inducing experimental autoimmune uveitis and the outer layer of retina is the sensitive target organ of S antigen.

本实验利用饱和硫酸铵盐析和葡聚糖凝胶过滤层析,从猪视网膜中提取了S抗原。50 μg S抗原伴等量完全佛氏佐剂,注射于豚鼠后足掌肉垫皮下。两周后,诱发了中度至严重的葡萄膜视网膜炎。其病理特征是:脉络膜内有大量淋巴细胞浸润;由前向后,逐渐增厚。炎症变化与病程有关。超微结构特征性改变是视网膜感光细胞内、外节水肿,变性,坏死。提示:S抗原有强烈诱发自身免疫性葡萄膜视网膜炎的作用;外层网膜是较敏感的靶器官。

The determination of lipid peroxide damage in the primary cultured pig retinal ganglion cells induced by microwave can provide some experience on the effect of microwave and the protection from its damage. Retinal ganglion cells were cultured in vitro and exposed to different intensities or time of microwave,and cultured for another 48 hours.The content of superoxide dismutase (SOD) and malondialdehyde (MDA) was detected.Results showed that the content of MDA was increased and SOD decreased after...

The determination of lipid peroxide damage in the primary cultured pig retinal ganglion cells induced by microwave can provide some experience on the effect of microwave and the protection from its damage. Retinal ganglion cells were cultured in vitro and exposed to different intensities or time of microwave,and cultured for another 48 hours.The content of superoxide dismutase (SOD) and malondialdehyde (MDA) was detected.Results showed that the content of MDA was increased and SOD decreased after radiation.After 48 hours in culture,the content of MDA recovered, and SOD increased.It is concluded that microwave induced the lipid peroxide damage in primary cultured retinal ganglion cells.These damages were recoverable in certain range of indensity or time.

目的:探讨微波对体外培养的猪视网膜神经节细胞的脂质过氧化损伤作用及耐受剂量,可为进一步研究微波的眼底损伤机理及其防护提供一定的实验依据。方法:体外培养猪视网膜神经节细胞,2450MHz连续波以不同强度和时间进行辐照,然后继续培养48小时,测定超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果:微波辐照后,细胞MDA含量明显增高,SOD降低,经过48小时再培养,MDA含量有所恢复,SOD含量增高。结论是:微波可引起视网膜神经节细胞脂质过氧化损伤,在一定剂量范围内为可逆性损伤。

AIM To investigate the protective effect of zincon the microwave induced lipid peroxide damage to in vitro cultured pig retinal ganglion cells and the cells' tolerance to microwave. METHODS Pig retinal ganglion cells were cultured in vitro and divided into 3 groups of different radiation intensities (control group, 30 mW·cm -2 group, 60 mW·cm -2 group) and 2 more radiated groups with different dosages of zinc added. The activity of intracellular superoxide dismutase (SOD) and...

AIM To investigate the protective effect of zincon the microwave induced lipid peroxide damage to in vitro cultured pig retinal ganglion cells and the cells' tolerance to microwave. METHODS Pig retinal ganglion cells were cultured in vitro and divided into 3 groups of different radiation intensities (control group, 30 mW·cm -2 group, 60 mW·cm -2 group) and 2 more radiated groups with different dosages of zinc added. The activity of intracellular superoxide dismutase (SOD) and the content of malondia ldehyde (MDA) of each group were assayed after they had been radiated for 1 h in a shielded room by 2450 MHz continuous wave physiotherapy machine. 0 and 72 h after radiation, the morphological variation of cells was observed by optical microscopy and the activity of intracellular SOD and the MDA content were determined.RESULTS After radiation, some cells congregated and their axons disappeared. The content of MDA increased significantly compared with that of the control group and whereas activity of SOD decreased. No obvious change was discovered in the zinc added groups by microscopy. The content of MDA recovered and activity of SOD in the zinc added groups increased compared with that of the radiated groups without zinc added. With cells cultured for 72 h after radiation, the content of MDA of the 30 mW·cm -2 group decreased and there was no significant difference between the MDA content of the 60 mW·cm -2 group and that of the control. MDA decreased whereas SOD increased in zinc added groups after the 72 h culture, compared with that in the radiation groups. CONCLUSION The results of this study indicate that microwave can induce lipid peroxide damage to in vitro cultured retinal ganglion cells and that Zinc can improve antioxidative capacity of cells, and to some extent can remedy microwave induced damage of lipid peroxidation to retinal ganglion cells. Besides, the effect of zinc is related to its action time.

目的 以锌作为防护因素研究微波对体外培养的猪视网膜神经节细胞的脂质过氧化损伤作用及耐受剂量 .方法 体外培养猪视网膜神经节细胞 ,按微波辐照强度不同分为对照组 ,30 m W· cm- 2 组 ,6 0 m W· cm- 2 组及各辐照剂量加锌组 ,微波理疗机于微波屏蔽室内辐照 1h,分别于辐照后 0和 72 h观察细胞形态变化 ,测定超氧化物歧化酶 (SOD)和丙二醛 (MDA)含量 .结果 微波辐照后 ,细胞有聚集现象 ,部分细胞轴突消失 ,细胞 MDA含量明显增高 ,SO D降低 ,加锌各组光镜下细胞形态变化不明显 ,MDA含量较未加锌组有所恢复 ,SOD活性也增高 ;细胞辐照后继续培养 72 h,30 m W·cm- 2 组 MDA含量降低 ,6 0 m W· cm- 2 组 MDA与辐照组无明显差别 ,加锌后再继续培养 72 h,则两组细胞 MDA均比辐照后降低 ,SOD活性增强 .结论 微波可引起视网膜神经节细胞脂质过氧化损伤 ,锌可提高细胞的抗氧化能力 ,在一定程度上减轻微波对视网膜神经节细胞的过氧化损伤 ,其作用效果与作用时间有关 .

 
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