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race strategy
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  竞赛策略
     Fudenberg's Model and the Patent Race Strategy of Chinese Enterprise
     弗得伯格模型与我国企业专利竞赛策略
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  “race strategy”译为未确定词的双语例句
     A novel 1886bp full-length sesquiterpene synthase (ASES) cDNA was cloned from a high-yield Artemisia annua strain 001 by RACE strategy.
     用RACE方法从青蒿高产株系001中克隆了一个新的1886bp的全长倍半萜合酶cDNA。
短句来源
     For the first time a 1539bp squalene synthase (ASQS) cDNA was cloned from a high-yield Artemisia annua strain 001 by RACE strategy.
     用RACE方法首次从青蒿中克隆了一个1539 bp全长鲨烯合酶cDNA。
短句来源
     and applied for patent, the apply number is 03105029.8.A Na+/H+ antiport full length cDNA was cloned from Tetragonia tetragonioides by RT-PCR and RACE strategy, and named TtNHX1 The cDNA is 2199bp in length including an open reading frame of 1665bp, 91bp 5' UTR, 413bp 3' UTR and 31bp Oligo d(T) tail.
     利用RT-PCR及RACE方法成功地从番杏中克隆Na~+/H~+逆向转运蛋白基因的全长cDNA,将其命名为TtNHX1。 该cDNA全长2199bp,包括1665bp的开放读码框(open reading frame,ORF),91个碱基的5′非翻译区(non translaed region,NTR),413个碱基的3,非翻译区和31个碱基的多聚腺苷酸尾。
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     Three nAChR subunits (Agα1, Agα2 and Agβ1) were cloned from Aphis gossypii with RT-PCR and RACE strategy. Both 5′ and 3′ cDNA ends of 3 subunits were successfully determined with 5′ and 3′-RACE techniques.
     采用RACE技术,成功地从棉蚜中克隆了3个nAChR亚基,其中2个为α亚型, 1个为β亚型,分别命名为Agα1、Agα2和Agβ1。
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     One cDNA whose corresponding mRNA was preferentially accumulated in rice florets was isolated by PCR-mediated RNA subtraction hybridization and RACE strategy. This gene, termed RA68 encoded a protein with proline- and threonine- rich motif.
     第一部分利用减法杂交和RACEs从水稻中克隆了一个编码含有脯氨酸和苏氨酸丰富结构域多肽的cDNA,其相应的基因被命名为RA68。
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  相似匹配句对
     Strategy on Velocity Distribution in Short Race
     短跑全程速度战术特征分析
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     Fudenberg's Model and the Patent Race Strategy of Chinese Enterprise
     弗得伯格模型与我国企业专利竞赛策略
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     WIN BY STRATEGY
     战略制胜
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     On Pricing Strategy
     透视经营者定价
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  race strategy
A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences.
      
A degenerate 3' RACE strategy resulted in the identification of fifteen novel P450s from an alkaloid-resistant species of Drosophila.
      
A 2 149 bp full length phytoene desaturase (PDS) cDNA was first cloned from saffron (Crocus sativus L.) stigma using RT-PCR technique and a rapid amplification of cDNA end (RACE) strategy.
      
We then used the RACE strategy to obtain the entire sequence of the channel.
      
With an additional 5 RACE strategy, the complete sequence encoding the signal peptide and 5untranslated region was deduced.
      
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A 1 886 bp full_length sesquiterpene synthase (AaSES) cDNA was cloned from a high_yield Artemisia annua L. strain 001 by a rapid amplification of cDNA end (RACE) strategy. AaSES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi _cedrol synthase from A. annua , 48% identical to amorpha_4, 11_diene synthase from A. annua , 39% identical to the 5_ epi _aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. muticus , 41%...

A 1 886 bp full_length sesquiterpene synthase (AaSES) cDNA was cloned from a high_yield Artemisia annua L. strain 001 by a rapid amplification of cDNA end (RACE) strategy. AaSES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi _cedrol synthase from A. annua , 48% identical to amorpha_4, 11_diene synthase from A. annua , 39% identical to the 5_ epi _aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. muticus , 41% identical to the δ_cadinene synthase from cotton. The coding region of the cDNA was inserted into a procaryotic expression vector pET_30a and overexpressed in E. coli BL21(DE3). The cyclase proteins extracted from bacterial culture were found largely in an insoluble protein fraction. AaSES expresses in leaves, stems and flowers, not in roots as indicated by Northern blotting analysis.

用RACE方法从青蒿 (ArtemisiaannuaL .)高产株系 0 0 1中克隆了一个新的 1886bp的全长倍半萜合酶cDNA。克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为 39%、38%和 4 1% ;与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC12 5的一致性为5 0 %、4 8%和 5 9%。cDNA编码区序列被克隆进原核表达载体pET_30a,并在大肠杆菌 (Escherichiacoli)BL2 1(DE3)中诱导表达 ,但过量表达的蛋白主要是以不溶性蛋白形式存在。Northernblotting分析表明此基因在茎、叶和花中表达 ,在根中没有表达。

A complete cDNA of potato Phytophthora infestans-induced hypersensitive response-related protein gene ( POTHR- f) was cloned using rapid amplification of cDNA ends (RACE) strategy according to a fragment sequence which we had cloned using suppression subtractive hybridization (SSH) technique. The potato POTHR-1 gene encodes a protein of 225 amino acids, which shares 81% identity with tobacco hin1 gene-enoded protein (harpin-induced protein). Southern blot revealed that there are two to three copies...

A complete cDNA of potato Phytophthora infestans-induced hypersensitive response-related protein gene ( POTHR- f) was cloned using rapid amplification of cDNA ends (RACE) strategy according to a fragment sequence which we had cloned using suppression subtractive hybridization (SSH) technique. The potato POTHR-1 gene encodes a protein of 225 amino acids, which shares 81% identity with tobacco hin1 gene-enoded protein (harpin-induced protein). Southern blot revealed that there are two to three copies of POTHR-1 in potato genome. The POTHR-1 gene expression in potato leaves showed that its transcripts accumulated remarkably in leaves after 36 h inoculation with P. infestans. Mechanical wounding and jasmonic acid (JA) could induce the POTHR-1 gene expression and osmotic stress just induce a slight accumulation of POTHR-1 gene mRNA, while salicylic acid (SA) had no detectable function on the induction accumulation of POTHR-1 gene transcripts. The potato POTHR-1 gene may preferentially associate with hypersensitive response (HR) or biotic cell death during interaction between host and pathogen.

利用RACE和重叠延伸相结合的方法,从经晚疫病菌接种诱导的马铃薯水平抗性材料叶片中克隆了一个POTHR-1基因(potato Phvtophthora infestans-induced hypersensitive response related protein gene)的全长cDNA。序列分析表明,该基因编码225个氨基酸,与烟草harpin诱导蛋白基因hinl有很高的同源性(编码区核苷酸和氨基酸序列分别为83%和81%)。Southern杂交结果显示在马铃薯基因组中有2~3个拷贝。对其诱导表达模式研究表明:晚疫病病原菌接种36h后,该基因表达迅速增加;机械伤害及茉莉酸(JA)处理能够诱导表达;渗透胁迫(NaCl浸泡)能够诱导其微弱表达;但水杨酸(SA)不能诱导表达。该基因可能和病原与寄主互作时寄主产生过敏反应及细胞生理性死亡有关。

Using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification ofcDNA ends (RACE) strategies, two chalcone synthase (CHS) cDNAs were cloned from developing seeds ofblue-grained wheat, both of the deduced peptides contain 394 amino acids, and share 98.9% of amino acidsequence identity and the nucleotide sequences have the identity of 96.0%, and one flavonoid 3'5'-hydroxylase (F3 '5 'H) 3'-end cDNA was isolated. Four CHS genomic DNAs were cloned from Thinopyrumponticum (Podp.)...

Using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification ofcDNA ends (RACE) strategies, two chalcone synthase (CHS) cDNAs were cloned from developing seeds ofblue-grained wheat, both of the deduced peptides contain 394 amino acids, and share 98.9% of amino acidsequence identity and the nucleotide sequences have the identity of 96.0%, and one flavonoid 3'5'-hydroxylase (F3 '5 'H) 3'-end cDNA was isolated. Four CHS genomic DNAs were cloned from Thinopyrumponticum (Podp.) Z. W. Liu et R. R.-C. Wang (ThpCHS.tg), blue-grained wheat (TaCHS.bg), white-grainedoffspring of light blue-grained wheat (TaCHS.wg) and Chinese Spring (2n=42)(TaCHS.csg), respectively.Although these four genomic DNAs were isolated from different materials, they are very high homologousand each has one intron. The difference of the four CHS genomic DNAs mainly exists in intron. ThroughDNA alignment we found that one CHS cDNA (TaCHS.t1) came from one of the parents, Th. ponticum, theother one (TaCHS.w1) had the identity of 100% with white grain parent. This indicated that CHS genes fromtwo parents expressed at the same developing stage in blue-grained wheat. Southern blotting analysisshowed that they have at least four copies in wheat, the copy numbers in different color grains are notsignificantly different, but they are different from that of Th. ponticum. CHS in blue-grained wheat belongsto a CHS multifamily. Reverse Northern analysis indicated that the CHS expressed strongly in thedeveloping blue-grained seeds at early stage (15 d after flowering, DAF), but F3 '5 'H and dihydroflavonol 4-reductase (DFR) transcripts accumulated less than that of CHS at early stage. However, at the laterdeveloping stage (21 DAF), F3 '5'H and DFR transcripts accumulated more than that of CHS, thetranscripts of CHS could hardly be detected. The expression order of the three genes is the same as theorder of the biosynthetic steps in anthocyanin biosynthesis. At the same time, CHS genes cloned fromseeds have not been detected in leaves of blue-grained wheat, but F3 '5 'H and DFR expressed strongly inleaves. This showed that the expression of CHS genes cloned by us had tissue specificity. RT-PCRindicated that the transcripts of F3 '5'H accumulated a lot in the developing seeds of blue- and white-grained wheats at 21 DAF, but the transcripts of CHS and DFR accumulated in the blue-grained wheat morethan those of white-grained wheat and Chinese Spring at the same developing stage. Therefore, weproposed that anthocyanin biosynthetic pathway existed in blue-grained wheat and the expression of thesecondary structure genes in anthocyanin biosynthetic pathway was coordinately regulated by regulatorygene(s) during the period of blue pigment formation.

采用RT-PCR和RACE方法从蓝粒小麦(TriticumaestivumL.×Thinopyrumponticum(Podp.)Z.W.LiuetR.R.-C.Wang)发育种子中克隆到两个查尔酮合酶基因(TaCHS.t1,TaCHS.w1),分别编码394个氨基酸,二者核苷酸和氨基酸序列的同源性分别为96.0%和98.9%;而且克隆到一个类黄酮3'5'-羟化酶基因(F3'5'H)3'-末端。分别从长穗偃麦草(Thinopyrumponticum(Podp.)Z.W.LiuetR.R.-C.Wang)、蓝粒小麦、白粒小麦和中国春基因组中分离到查尔酮合酶基因(CHS)的全长序列(ThpCHS.tg,TaCHS.bg,TaCHS.wg,TaCHS.csg),它们的核苷酸序列之间具有很高的同源性,且均含有一个内含子(intron),序列差异主要在内含子。通过DNA序列比较,发现一个CHS与亲本之一的长穗偃麦草基因组同源性达100%,一个CHS与另一白粒亲本基因组同源性达99%,表明来自于父、母本的CHS在蓝粒发育的种子中均表达。Southern杂交结果表明CHS在小麦中的拷贝数至少有4个,不同颜色的蓝粒小麦、白粒...

采用RT-PCR和RACE方法从蓝粒小麦(TriticumaestivumL.×Thinopyrumponticum(Podp.)Z.W.LiuetR.R.-C.Wang)发育种子中克隆到两个查尔酮合酶基因(TaCHS.t1,TaCHS.w1),分别编码394个氨基酸,二者核苷酸和氨基酸序列的同源性分别为96.0%和98.9%;而且克隆到一个类黄酮3'5'-羟化酶基因(F3'5'H)3'-末端。分别从长穗偃麦草(Thinopyrumponticum(Podp.)Z.W.LiuetR.R.-C.Wang)、蓝粒小麦、白粒小麦和中国春基因组中分离到查尔酮合酶基因(CHS)的全长序列(ThpCHS.tg,TaCHS.bg,TaCHS.wg,TaCHS.csg),它们的核苷酸序列之间具有很高的同源性,且均含有一个内含子(intron),序列差异主要在内含子。通过DNA序列比较,发现一个CHS与亲本之一的长穗偃麦草基因组同源性达100%,一个CHS与另一白粒亲本基因组同源性达99%,表明来自于父、母本的CHS在蓝粒发育的种子中均表达。Southern杂交结果表明CHS在小麦中的拷贝数至少有4个,不同颜色的蓝粒小麦、白粒小麦间拷贝数基本一致,但都与长穗偃麦草有差异,根据以上结果初步判定CHS在蓝粒小麦中属于一个多基因家族。Re-verseNorthern分析表明CHS在开花后15d发育的蓝粒小麦中具有较强的表达;花后21dF3'5'H和DFR的转录本积累显著高于CHS,基本上检测不到CHS的表达。这三个基因在蓝粒小麦中的表?

 
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