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green monkey
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     Molecular Cloning of Highly Reiterated Sequence AGMr (HindⅢ)-1in Vero Cell Line of African Green Monkey and Its Preliminary Application
     非洲绿猴肾细胞株AGMr(HindⅢ)-1高度重复顺序的克隆及初步应用
短句来源
     Methods: The cDNA of African green monkey's LR was amplified from total RNA of Vero-E6 cells and was ligated into pGEM-T Easy plasmid.
     方法:采用RT-PCR从Vero-E6细胞总RNA中扩增非洲绿猴LR的cDNA序列,克隆到pGEM-TEasy载体上;
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     SARS-CoV Receptor ACE2 from African Green Monkey Kidney Cells, cDNA Cloning, Expression and Identification of Receptor Function Domain
     非洲绿猴肾细胞中SARS冠状病毒受体血管紧张素转换酶2cDNA的克隆、表达及功能区的鉴定
短句来源
     HCV can not bind the CD81 of African Green Monkey(AGM). Four amino acids(163, 186, 188, 196) locating in EC2 of CD8I are different between human and AGM.
     非洲绿猴(AGM)的CD81不与HCV结合,而它和人的CD81有四个氨基酸(163,186,188,196)不同,位于EC2环,对应于CD81基因的第6、7外显子。
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     Its identi ty wi th the MT-II of green monkey was 41% and that with the human MT-II was 35%.
     与哺乳动物绿猴MT-Ⅱ的同源性达到41%,与人类MT-Ⅱ的同源性为35%。
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  “green monkey”译为未确定词的双语例句
     Methods pVAX1-GRA8 was purified and transfected into Africa green monkey kindey vero cell lines,expression of GRA8 in vero cell lines were detected by RT-PCR and immunoblot with rabbits sera infected with Toxoplasma gondii.
     方法:大量提取纯化重组真核表达质粒pVAX1-GRA8,瞬时转染培养的vero E-6细胞,RT-PCR和免疫印迹法检测GRA8在细胞中的表达。
短句来源
     Objective: Our aim was to purity the modifier protein of glyceraldehyde 3 phosphate dehydrogenase (G3PD) from African green monkey Vero E6 line.
     目的 :从猴的肾上皮细胞 (Vero- E6 )内提取具有促进细胞生长作用的 3-磷酸甘油醛脱氢酶 (G3PD)的修饰蛋白 (m odifier protein,MP)。
短句来源
     Methods Site-directed mutagenesis was conducted to obtain 2 mutant human XIII A recombinant plasmids, mut-PCI/FXIIIA. Normal wild type factor XIII A recombinant plasmid, wt-PCI/FXIIIA, and mut-PCI/FXIIIA, were transfected into cultured COS7 cells line, renal fibroid cell of African green monkey using Superfect reagent respectively, The expression levels of DNA, RNA and protein of human factor XIII, both wild type and mutant, were detected by PCR, RT-PCR and Western blotting.
     方法 构建正常人FXIIIA重组表达质粒 (wt PCI/FXIIIA) ,通过定点突变获得上述 2种突变的FXIIIA重组表达质粒 (mut PCI/FXIIIA) ,并分别将它们转染到COS7细胞表达 ,PCR、RT PCR和Western印迹检测转染细胞中人FXIIIA的DNA水平、RNA水平及其蛋白质的表达量。
短句来源
     This study was undertaken to compare sensitivity of buffulo green monkey(BGM) cells, Hela cells, human embryo lung(HEL) cells and A549 cells to herpes simplex virus 1. The plague forming unit(PFU) technique was used to measure the four cell lines simultaneously.
     用单纯疱疹病毒-1型(HSV-1)感染BGM细胞、Hela细胞、HEL细胞及A549细胞,利用空斑试验,比较四种细胞对HSV-1的敏感性。
短句来源
     ricin were Purified by a Sepharose 4B column and gel filtration on a Sephadex G-100. The Africa green monkey kideny cellline Vero were treated with raw Stx2e and ricin .
     方法:用硫酸铵沉淀法制备Stx2e突变体粗毒素; 经Sephrose 4B柱亲和层析和SephadexG—100凝胶过滤,分离得到ricin。
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  相似匹配句对
     Green
     绿色情思
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     Green
     颜色文化——绿色(英文)
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     THE MONKEY
     猕猴猴趣
短句来源
     The Monkey and the Crocodile
     中学视听法教学示例《猴子与鳄鱼》
短句来源
     Cloning,Expression and Purification of African Green Monkey Laminin Receptor
     非洲绿猴层黏连蛋白受体的克隆、表达及纯化
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  green monkey
The structure of the green monkey Chlorocebus aethiops heparin-binding EGF-like growth factor (HB-EGF) gene was compared with that of the corresponding human gene.
      
Escherichia coli with both P and type 1 fimbriae caused vaginal colonization in the female green monkey, while only the P-fimbriated bacteria frequently caused ascending bladder infection.
      
The HEV variants found were cultured in a continuous culture of green monkey kidney cells (4647).
      
Transactivation of progestin- and estrogen-responsive promoters by 19-nor progestins in african green monkey kidney CV1 cells
      
Cultures of fetal aoudad sheep kidney (FAK), bovine embryonic lung (BEL), and African green monkey kidney (Vero) cells were compared for differential replication of alcelaphine herpesviruses.
      
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This paper presents a transient expression of the enzyme chloramphenicol acetyl transferase (CAT) in mammalian cell. The expression of cat gene in mammalian cells permits an easy assay for promoters in eukaryotic vectors. Meanwhile the CAT assay system provides a rapid and accurate method for comparing different promoter, measuring the apparent strength of one promoter in a number of cell type, comparing transfection protocols and evaluating other parameters affect exogenous gene expression.The prototype vecter...

This paper presents a transient expression of the enzyme chloramphenicol acetyl transferase (CAT) in mammalian cell. The expression of cat gene in mammalian cells permits an easy assay for promoters in eukaryotic vectors. Meanwhile the CAT assay system provides a rapid and accurate method for comparing different promoter, measuring the apparent strength of one promoter in a number of cell type, comparing transfection protocols and evaluating other parameters affect exogenous gene expression.The prototype vecter pSV2cat utilises the SV40 promoter to direct transcription of this gene. Readily measured leveles of CAT acumulated within 48k after the introduction of pSV2cat DNA into African green monkey kidney CV1 cells. Because endogenous CAT activity is not present in CVl cells or other mammalian cells and because rapid sensitive assay, which follows the conversion of [14C]chloramphenicol to its acetylation derivatives by thin layer chro- matography for CAT activity is available. To demonstrate the usefulness of this system we constructed derivative of pSV2cat, The 551 bp Ha-ras gene promoter has been substituted for SV40 early promoter region. The pHrasCAT constructed also retained function in CAT synthesis in CV1 cells

本文介绍一种快速方便的外源基因在哺乳动物细胞中瞬间表达检测系统,即氯霉索乙酰转移酶检测系统。该项检测技术不仅对基因的调控顺序(Cis-acting elements)如启动子、增强子等检测提供了有效的工具,而且为真核基因在哺乳动物细胞表达体系中,质粒的构建及筛选提供了行之有效的手段。并介绍了该技术的全过程及其操作要点。

Two animal rotaviruse strains,Simian rotavirus SA11(serotype 3)and calf rotavirus NCDV(serotype 6)were pass,aged on BGMK cell(a africa green monkey kideny cell line)at 36℃,33℃ and 29℃ respectively Hemoagglutinin activities of the rotaviruses passaged at lower temperature decreased obviously.Infectivities of rotaviruses passaged at lower temperature in cell culture at 39℃ were lower than those of the original strains. The results of sero-neutralization test showed that after passages through 50 times,the...

Two animal rotaviruse strains,Simian rotavirus SA11(serotype 3)and calf rotavirus NCDV(serotype 6)were pass,aged on BGMK cell(a africa green monkey kideny cell line)at 36℃,33℃ and 29℃ respectively Hemoagglutinin activities of the rotaviruses passaged at lower temperature decreased obviously.Infectivities of rotaviruses passaged at lower temperature in cell culture at 39℃ were lower than those of the original strains. The results of sero-neutralization test showed that after passages through 50 times,the two strains could still be neutralized by antibody to original strains.PAGE analysis indicated that SA11 remained their genetic stability throughout the study,while the fifth segment of the genome RNA of NCDV was changed.

本文报道了动物轮状病毒(RV)SA_(11)及 NCDV 株经低温传代后生物学特性变化。这两个毒株分别属动物 RV 第3和第6血清型。本实验室用非洲缘猴肾细胞(BGMK)作为基质,将两株病毒连续传代,选择起始温度36℃,每传10代降低3℃,最终在29℃传20代,总传代数为50代。低温传代后的毒株,血凝活性明显下降,对组织培养感染性在39℃时,冷适应株低于原始株,血清中和试验表明:传代后毒株抗原性稳定,SA_(11)株具有良好的遗传稳定性。NCDV 株在传代过程中第5基因片段发生变异。

For analysing the injurious mechanismof Trichosanthin(TCS)on trophoblastcells,cytotrophoblast cells were separatedfrom placental villi in human early pergnan-ey and cultured on millipore filters coatedwith collagen in dual-environmental culturechambers.After 7—10 days culture thecells grew into confluent monolayer.A lotof multinucleated glant cells(synthetiallike)could be found under light microsco-py.Cytotrophoblast cells and the glant onewere characterized as epithelial type byindirect immuno-fluorescent staining...

For analysing the injurious mechanismof Trichosanthin(TCS)on trophoblastcells,cytotrophoblast cells were separatedfrom placental villi in human early pergnan-ey and cultured on millipore filters coatedwith collagen in dual-environmental culturechambers.After 7—10 days culture thecells grew into confluent monolayer.A lotof multinucleated glant cells(synthetiallike)could be found under light microsco-py.Cytotrophoblast cells and the glant onewere characterized as epithelial type byindirect immuno-fluorescent staining withanti-keratin.Because the procedure of se-paration of trophoblast cells is laboriousand the choriocarcinoma cells(JAR)wereas sensitive as trophoblast cells to tricho-santhin,so the choriocarcinoma cells wereused instead of the trophoblast cells in thelater experiments.The internalization and distribution ofTCS conjugated to 15nm(in diameter)gold particles were examined.Electron mi-croscopy showed that the TCS-gold parti-cles were bound to the cell membrane andentered via coated pit and then internal-ized into coated vesicles(endosomes)within30—60 minutes after treatment.Neverthe-less,a number of free TCS-gold particlesentered into the cell membrane nonspecifi-cally.In the series of 60—120 minutestreatment,the TCS-gold particles presentedin the multivesicular bodies.It is worthyto emphasize that the TCS-gold particlesentered the cytosol from the endosomes anddistributed nearby the rough endoplasmicreticulum(RER)and ribosomes within120—180 minutes.In the meanwhile,thecells showed pathological changes markedly.With the same method,human livercarcinoma cells(BE 7402),kidney cells ofAfrican green monkey(CV-1)and rat em-bryonic liver epithelial cells(LW 13)weretreated by TCS-gold particles as control.However,no particles had been found onthe cell surface or in the cytosol within60—120 munites(see Table 1).In addition,trophoblast cells were treated by BSA-goldparticles and transferrin-gold particles se-parately there still no particles could befound,(See Table 2).It is more interes-ting that TCS-Hepama-1-gold particlesinternalized into the choriocarcinoma cellswith the same manner as TCS-gold entered,and it could not be affected by pretreat-ment with Hepama-1 an hour.But,TCS-Hepama-1-gold particles bound to themicrovilli of human liver carcinoma cells,and it can be inhibited competitively bypretreatment with Hepama-1 for an hour(see Table 3).These results indicated consistentlythat trichosanthin possesses a high affinityto the membrane of trophoblast cells andchoriocarcinoma cells,and the internaliza-tion of this plant toxic protein into itstarget cells characterized by receptor-me-diated endoeytosis.The breakdown of theendosomes might be caused by acidification,and the pathway of TCS-gold particlestranslocated into the cytosol of target cellswere discussed preliminarily.

在体外培养条件下,天花粉蛋白胶体金以受体介导的内吞方式进入滋养层细胞和绒癌细胞,最终进入胞质溶胶附着在核糖体上。经相同处理的肝癌细胞,绿猴肾细胞和正常鼠胚肝细胞,金颗粒既不与这些细胞表面结合,也没有被专一内吞的现象。分别用BSA,转铁蛋白活化的胶体金处理滋养层细胞和绒癌细胞的比较观察,也证明滋养层细胞和绒癌细胞对天花粉蛋白的专一亲和性。更有趣的是:天花粉蛋白肝癌单抗胶体金结合物进入绒癌细胞的方式与天花粉蛋白的结果相同;先以旰癌单抗处理也不影响天花粉蛋白肝癌单抗结合物进入绒癌细胞。然而,对于旰癌细胞,天花粉蛋白肝癌单抗胶体金颗粒则专一地结合在细胞的微绒毛表面,这种结合因先以肝癌单抗处理而明显地被竞争抑制。这些实验结果相互印证地提供天花粉蛋白对滋养层细胞和绒癌细胞高度专一的亲和性,并证明天花粉蛋白对靶细胞的原初损伤部位是核糖体。进一步探讨天花粉蛋白在靶细胞表面结合位点的化学性质以及这种蛋白在靶细胞内的转运机制将是重要的问题。

 
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