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forming cells
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  形成细胞
    When the oral dose was 4.5g/kg in mouse the weight of spleen increased by 25.8%; the amount of antibody forming cells (AFC) increased obviously.
    小鼠口服益肝片4.5g/kg,脾脏相对重量增加25.8%,抗体形成细胞(IgM-PFC)明显增加;
短句来源
    Aim and Methods: High proliferative potential colony forming cells (HPP CFC) as one of the most primitive hematopoietic progenitors with CD34 + DR - Lin - antigens have been identified and used as effective tools for examination of functional properties of hematopoietic stem cells.
    目的和方法:高增殖潜能集落形成细胞(HPPCFC)是表达CD34+DRLin-的最早期造血祖细胞之一,它在体外的增殖分化能力可反映造血干细胞的某些特征。
短句来源
    Objective:To evaluate the changes of colony forming cells with high proliferative potential (HPP CFC) in human CD34 + cell culture after in vitro amplification.
    目的 :评价CD34 +细胞体外扩增时高增殖潜能集落形成细胞 (HPP CFC)的变化。
短句来源
    Transduction efficiency (20.5%) in the presence of bone marrow stromal cells plus cytokines(SCF+FL+IL 3) was higher than that (15.2%) without stromal cells, and the number of drug resistant colony forming cells was more in the former than in the latter.
    骨髓基质细胞 +细胞因子 (SCF +FL +IL 3)支持的基因转导效率 (2 0 .5 % )又高于单纯用该组细胞因子的转导效率 (15 .2 % ) ,并且前者形成的抗性集落形成细胞数多于后者。
短句来源
    EFFECTS OF LITHIUM ON THE PROLIFERATION OF MURINE HIGH PROLIFERATIVE POTENTIAL COLONY FORMING CELLS AND GRANULOCYTE MACROPHAGE COLONY FORMING UNIT IN VITRO
    锂对小鼠高增殖潜能集落形成细胞和粒巨噬系祖细胞体外增殖的影响
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  “forming cells”译为未确定词的双语例句
    Preliminary Study on Hog E-Rosette Forming Cells and ANAE Positive Cells
    猪E玫瑰花细胞与酯酶染色阳性细胞的初步研究
短句来源
    STUDIES ON THE HETEROGENITY OF SPLEEN COLONY FORMING CELLS
    脾结节生成细胞群的不均一性研究
短句来源
    Age-related Changes in Antibody Forming Cells (B Cells)
    B细胞产生抗体的年龄相关性变化
短句来源
    The percentage of rosette forming cells was 3.24±0.90%. Through density gradient separation, LC-enriched (Se) and LC-depleted (Sd) epidermal cell suspensions were obtained. Samples of S and Se, Sd were applied simu taneously to mixed culture with another individual's lymphocytes.
    在表皮细胞(S)悬液中,应用EAIgG与郎罕细胞结合成花环,花环形成率为3.24±0.90%,并通过密度梯度分离,得到了富有郎罕细胞的表皮细胞(Se)和缺乏郎罕细胞的表皮细胞(Sd)悬液。
短句来源
    CD 34 - cell population from cord blood MNC contained hematopoietic stem/progenitor cells and the cytokines secreted by CD 34 - cells could induce CD 34 + cells to more mature colony forming cells.
    脐血MNC中大量的CD34- 细胞含有造血干 祖细胞 ,其分泌的细胞因子有促进CD34+ 细胞向较为成熟的集落形成祖细胞分化的作用。
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  forming cells
coli cells with the constructs containing SP was accompanied by the disruption of the forming cells.
      
Over the studied period of time, Renobacter vacuolatum cells retained viability at a low temperature, whereas, at room temperature, the titer of colony-forming cells decreased by two orders of magnitude under starvation stress.
      
The GA glycopeptide with three residues of Glu(OH)-OMe at a dose of 2 mg/kg stimulated the production of antibody-forming cells in mouse spleen in comparison with the control.
      
The removal of brain in utero by decapitation of 18-day fetuses induced a fourfold increase in the number of antibody-forming cells in the liver, as compared to the unoperated fetuses.
      
After the removal of the forebrain, including hypothalamus (encephalectomy), the number of antibody-forming cells was comparable to that in unoperated fetuses.
      
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An improved plaque-forming assay for the detection of antibody-forming cells was reported in this paper.Onthe basis of Cunningham's slide cham-ber,improvements have been made inthe making of the chambers and thecounting of the plaques.Cover glassstripes in place of double sided self-adhesive tape were put between twoslides.The edges were sealed withparaffin (Fig.1),The chambers thusformed were filled up with a mixture,including spleen cells,SRBC,comple-ments,etc.On counting,a cover glasswith counting...

An improved plaque-forming assay for the detection of antibody-forming cells was reported in this paper.Onthe basis of Cunningham's slide cham-ber,improvements have been made inthe making of the chambers and thecounting of the plaques.Cover glassstripes in place of double sided self-adhesive tape were put between twoslides.The edges were sealed withparaffin (Fig.1),The chambers thusformed were filled up with a mixture,including spleen cells,SRBC,comple-ments,etc.On counting,a cover glasswith counting grid was put on thesurface of the chamber.According tothe area of the cover glass,the heightof the chamber,and the concentrationof spleen cells used,the number ofPFC in spleens can be calculated.Through the study of the kineticsof anti-DNP antibody-forming cells ofrats,it was shown that the methoddescribed here was simple and reliable.Methods of coupling hapten to SRBCand some influences of complementsand anti-IgG antiserum in this exper-iment were discussed.

本文报道了一种改良的检出抗体生成细胞的溶血空斑技术。在Cunningham 玻片小室法基础上,对玻片小室的制作,空斑的计数等方面进行了改进。通过对大鼠抗DNP 半抗原的抗体生成细胞的动力学检测,表明本文所述方法是简便可行的。此外对半抗原与SRBC 的连结和影响实验的一些因素——补体、抗TgG 抗血清等进行了探讨。

The spleen cells taken from Balb/ cJ mice 3 days after immunization with PDRBC were fused with NS-1 myelo-ma cells by two different procedures in the presence of PEG.The hybridcells secreting the specific antibody were selected out in the HAT medium by means of the spot test of the culture fluid.(Table 1).The antibody secre-ting hybrid cells were then cloned by either...

The spleen cells taken from Balb/ cJ mice 3 days after immunization with PDRBC were fused with NS-1 myelo-ma cells by two different procedures in the presence of PEG.The hybridcells secreting the specific antibody were selected out in the HAT medium by means of the spot test of the culture fluid.(Table 1).The antibody secre-ting hybrid cells were then cloned by either limiting dilution or micromani-pulation methods and a cell line thus obtained was designated as CBH-1.After ten months of culture in vitro up to 69 generations,the ability of this cell line to secret specific monoclonal antibody to PDRBC was not decreased. The CBH-1 cells were also ino-culated into the peritoneal cavity of Balb/cJ or Balb/cCr mice and could be kept in these mice for at least four transplantation generations.The ability of three different agents for inducing the ascitic fluid was compared.As a result,pristane was found to be superior to liquid paraffin and Arlacel A. Attempt was also made to cultureCBH-1 in serum-free medium instead of the conventional medium containing 20% newborn calf serum (NCS).When the serum-free medium was provided with 5γ transferrin per ml,the CBH-1 cells could be maintained just as well as that in DMEM+20% NCS within 15 days (Table 2) The proportion of plaque forming cells after the first cloning was found to be 97.5% and this was increased to 99.8% after second cloning (Table 3).This result implies that the cell popula-tion of the CBH-1 clone was very ho-mogeneous with respect to the secre-tion of the specific antibody to PDR-BC.

以北京鸭红血球为抗原免疫Balb/cJ小鼠,三天后取脾细胞与小鼠NS-1骨髓瘤细胞在PEG作用下,用两种方法进行细胞融合。只有淋巴细胞杂交瘤细胞能在一种选择性培液——HAT液中生长,具有分泌抗体活性的杂交瘤细胞,借助其上清液的斑点试验加以选择。对斑点试验阳性的杂交瘤细胞进行克隆化和扩大培养,得到能专一分泌抗北京鸭红血球单克隆抗体的淋巴细胞杂交瘤,定名为CBH-1。在体外经过69代培养,历时十月余,分泌能力仍未见衰减。体内可传4—5代,腹水中可检出大量分泌抗体。用降植烷、Arlacel A和石蜡油诱发动物的腹水,其量以降植烷最高、石蜡油次之。使用国产石蜡油作为诱发腹水试验似具有一定实用价值。加5γ转铁蛋白至每毫升不含牛血清的DMEM液中,在15天的培养期内,结果至少与加20%小牛血清的培液的结果相似。首次克隆化后的CBH-1细胞中,空斑形成细胞达97.5%,再次克隆化的细胞中,空斑形成细胞达99.8%,证明是均一的细胞群落。本文还就杂交瘤制备过程及细胞稳定性问题进行了讨论。

The purpose of this paper is to study kinetics and specificity of stable E rosette forming cells (Es-EFC) in typhoid infection. A total of 16 typhoid patients (10 children and 6 adults) were included in this study. Among these, 12 had blood taken twice (on day of admission and at discharge), the remaining 4 cases and a control group of 10 normal individuals only once. The heparinized blood specimens were incubated in presence of soluble antigen of S. typhi for 7 days in 5% CO2 incubator and were assayed...

The purpose of this paper is to study kinetics and specificity of stable E rosette forming cells (Es-EFC) in typhoid infection. A total of 16 typhoid patients (10 children and 6 adults) were included in this study. Among these, 12 had blood taken twice (on day of admission and at discharge), the remaining 4 cases and a control group of 10 normal individuals only once. The heparinized blood specimens were incubated in presence of soluble antigen of S. typhi for 7 days in 5% CO2 incubator and were assayed for Et-RFC and Es-RFC both before and at the termination of incubation. During the course of 7 days cultivation of 4 blood specimens, aliquots were removed on alternate days for examination of cell morphology and viability and for enumeration of Et-RFC and Es-RFC. Another 7 blood specimens were cultivated in presence of soluble antigen of E. coli. or S. typhi. so as to investigate the question of specificity. The results obtained revealed the f ollowing:(1) The rise in number of Es-RFC during typhoid infection was specific in nature (2) Under stimulation by specific antigen, both in vivo and in vitro, increase of Es-RFC occured quite early (as evident from in vitro experiments) and declined to normal level shortly after subsidence of clinical symptoms, suggesting the rise in Es-RFC might reflect the presence of high level of specific antigens (bacteremia, viremia, or toxemia) in circulation, and (3) Source of newly formed Es-RFC did not seem to result from cellular multiplication but rather due to activation of effector cells in presence of specific antigen, accompanied by marked increase of E receptors on cell surface.

本文的目的是观察伤寒感染中Es-RFC%的动态变化及其特异性。病人共16例。检测项目包括血液与伤寒抗原共育7天前后观察Et-和Es-RFC%。4份血标本用于检查T细胞形态、存活力和Et与Es-RFC%的动态变动。另7份血液用于观察Es-RFC的特异性。结果如下:(1)伤寒感染中Es-RFC的增多具特异性,(2)在伤寒抗原刺激下Es-RFC升高的出现相当早,病愈后消失也较快,提示ES-RFC的增多与血流中存在高水平特异性抗原有关,和(3)在特异性抗原刺激下ES-RFC数量的增多是致敏淋巴细胞表面E-受体大量形成所致。

 
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