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forming cells
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  形成细胞
    Objective:To investigate the colony formation capacity and P16 gene mRNA expression of high proliferative potential colony forming cells (HPP-CFC) derived from bone marrow hematopoietic cells of psoriatic patients,and probe into the relationship between the colony formation and P16 promoter mRNA expression.
    目的:研究银屑病患者骨髓高增殖潜能集落形成细胞(HPP-CFC)的集落形成能力及P16基因mRNA表达,探讨银屑病患者骨髓造血干细胞体外增殖活性及原因。
短句来源
    the bone marrow mononuclear cells were cultured. in methycellulose semi-solid culture medium with SCF,GM-CSF,IL-3 and IL-6 for 14 days, and then high–proliferative potential colony–forming cells(HPP-CFC) were counted. The HPP-CFC were collected and its genomic DNA was isolated .
    方法:密度梯度离心法分离银屑病患者及正常对照骨髓单个核细胞,培养于含SCF+GM-CSF+IL-3+IL-6细胞因子组合的甲基纤维素半固体培养基,培养14d时计数高增殖潜能集落形成细胞(HPP-CFC)集落,然后收集集落。
短句来源
    Methods Marrow mononuclear cells were isolated from bone marrow of psoriatic patients and normal controls by density gradient centrifugalization,and colony forming assays of high proliferative potential colony forming cells(HPP-CFCs),granulocyte-macrophage colony-forming units(CFUs-GM) and erythroid colony-forming units(CFUs-E) were performed in vitro in methylcellulose semi-solid culture medium in the presence of cytokines.
    方法采用密度梯度离心法分离银屑病患者及正常对照组骨髓单一核细胞,然后在含有甲基纤维素的半固体细胞因子培养体系中进行体外高增殖潜能集落形成细胞(HPP-CFC)、粒-巨噬细胞系集落形成单位(CFU-GM)及红系集落形成单位(CFU-E)集落形成实验,培养一定时期后计数集落。
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    Mutation frequency in 106 colony forming cells was increased from 20.4 ± 6.7 at 20 mJ/cm2 to 97.7 ± 7.1 under 60 mJ/cm2 of UVB irradiation. The expression level of ATP mRNA was down-regulated after UV irradiation.
    在每106个突变克隆细胞中突变频率由20mJ/cm2的(20.4±6.7)个增加到60mJ/cm2的(97.7±7.1)个。
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  forming cells
coli cells with the constructs containing SP was accompanied by the disruption of the forming cells.
      
Over the studied period of time, Renobacter vacuolatum cells retained viability at a low temperature, whereas, at room temperature, the titer of colony-forming cells decreased by two orders of magnitude under starvation stress.
      
The GA glycopeptide with three residues of Glu(OH)-OMe at a dose of 2 mg/kg stimulated the production of antibody-forming cells in mouse spleen in comparison with the control.
      
The removal of brain in utero by decapitation of 18-day fetuses induced a fourfold increase in the number of antibody-forming cells in the liver, as compared to the unoperated fetuses.
      
After the removal of the forebrain, including hypothalamus (encephalectomy), the number of antibody-forming cells was comparable to that in unoperated fetuses.
      
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Objective To investigate the effects of ultraviolet irradiation on the morphology, cell proli-feration, mutation frequency and level of expression of ATP mRNA in human fibroblasts. Methods The infant foreskin fibroblasts were cultured and irradiated with different doses of ultraviolet A or B (UVA or UVB). Light microscopy was used for observing the morphologic change and cell counting of cell proliferation, HPRT-mutagenesis assay for the mutation detection, and RT-PCR for the expression of ATP mRNA. Results...

Objective To investigate the effects of ultraviolet irradiation on the morphology, cell proli-feration, mutation frequency and level of expression of ATP mRNA in human fibroblasts. Methods The infant foreskin fibroblasts were cultured and irradiated with different doses of ultraviolet A or B (UVA or UVB). Light microscopy was used for observing the morphologic change and cell counting of cell proliferation, HPRT-mutagenesis assay for the mutation detection, and RT-PCR for the expression of ATP mRNA. Results Compared with the control group, the irradiated cultured fibroblasts were damaged, cell growth was retarded (MTT assay showed 2.4 fold increase in the control group but only 0.2 fold in the UVB group 72 h after irradiation). Mutation frequency in 106 colony forming cells was increased from 20.4 ± 6.7 at 20 mJ/cm2 to 97.7 ± 7.1 under 60 mJ/cm2 of UVB irradiation. The expression level of ATP mRNA was down-regulated after UV irradiation. There was an increase of mutation frequency which was UVB dose-dependenct, and decrease of ATP mRNA expression which was dose- and time-dependent. Conclusions After UV irradiation of the cultured fibroblasts, the cell damage and growth inhibition are induced, and the mutation frequency is increased in a UVB dose-depen-dent maaner. The expression of ATP mRNA shows down-regulation in both dose- and time-dependent manner.

目的研究紫外线(UV)照射对皮肤成纤维细胞形态学、生长增殖状态、突变频率和ATPmRNA表达水平的影响。方法采用一定剂量的中长波紫外线照射培养的新生儿包皮成纤维细胞。通过细胞计数记录细胞增殖,光学显微镜观察形态学变化,次黄嘌呤磷酸核糖转移酶突变实验检测突变频率,RT-PCR法检测ATPmRNA表达水平。结果成纤维细胞经UVB照射后,形态受损,细胞增殖减缓,照射72h后细胞活性只增加了约0.2倍,而非照射组增加约2.4倍。在每106个突变克隆细胞中突变频率由20mJ/cm2的(20.4±6.7)个增加到60mJ/cm2的(97.7±7.1)个。ATPmRNA表达水平随照射剂量及照射时间增加而呈下调变化。结论UV照射可使人成纤维细胞ATPmRNA表达水平呈剂量及时间依赖性下调变化,在能量消耗条件下成纤维细胞形态异常,生长受抑,突变频率与照射剂量的增加成正相关。

Objective To study the influence of culture supernatant of psoriatic peripheral blood mononuclear cells (PBMCs) on the colony-forming of bone marrow-derived hematopoietic stem cells and progenitor cells. Methods Bone marrow-derived mononuclear cells were separated by density gradient centrifugation. Methylcellulose semi-solid culture medium was used to culture the bone marrow mononuclear cells in culture systems. The PBMC culture supernatant from psoriatic patients or normal controls were added to the culture,...

Objective To study the influence of culture supernatant of psoriatic peripheral blood mononuclear cells (PBMCs) on the colony-forming of bone marrow-derived hematopoietic stem cells and progenitor cells. Methods Bone marrow-derived mononuclear cells were separated by density gradient centrifugation. Methylcellulose semi-solid culture medium was used to culture the bone marrow mononuclear cells in culture systems. The PBMC culture supernatant from psoriatic patients or normal controls were added to the culture, to observe their influence on the marrow-derived high proliferative potential colony forming cells (HPP-CFCs), erythroid and granulocyte-macrophage colony forming units (CFUs-E and CFUs-GM) of bone marrow hematopoietic stem/progenitor cells from healthy individuals. Results The values of HPP-CFCs, CFUs-E and CFUs-GM were significantly lower in the bone marrow cells stimulated by the supernatant of cultured psoriatic PBMCs than those stimulated by the supernatant of cultured normal PBMCs and those in the spontaneous proliferation group (P < 0.05, P < 0.01); however, no significant difference of those values was found between the group stimulated by supernatant of cultured normal PBMCs and the spontaneous proliferation group (P > 0.05). Conclusions Psoriatic PBMCs have specific biological activity and can inhibit the colony forming of bone marrow hematopoietic cells from healthy individuals.

目的研究银屑病患者外周血单一核细胞(PBMC)的培养上清液对骨髓造血干细胞、祖细胞集落形成的影响。方法密度梯度离心法分离骨髓单一核细胞,采用甲基纤维素半固体集落培养法研究银屑病患者及正常人PBMC培养上清液对骨髓高增殖潜能集落形成细胞、红系集落形成单位及粒细胞-巨噬细胞系集落形成单位集落形成的影响。结果银屑病患者PBMC培养上清液作用后,骨髓高增殖潜能集落形成细胞、红系集落形成单位及巨噬细胞系集落形成单位集落形成数较自然增殖组及正常人PBMC培养上清液组显著减少(P<0.05,P<0.01);而正常人PBMC培养上清液作用组与自然增殖组差异无统计学意义(P>0.05)。结论银屑病患者PBMC培养上清液对骨髓造血干细胞、祖细胞集落形成可能有影响。

Objective:To investigate the colony formation capacity and P16 gene mRNA expression of high proliferative potential colony forming cells (HPP-CFC) derived from bone marrow hematopoietic cells of psoriatic patients,and probe into the relationship between the colony formation and P16 promoter mRNA expression. Methods:Bone marrow-derived mononuclear cells from the psoriatics and normal controls were purified by density gradient centrifugation. The bone marrow mononuclear cells were cultured...

Objective:To investigate the colony formation capacity and P16 gene mRNA expression of high proliferative potential colony forming cells (HPP-CFC) derived from bone marrow hematopoietic cells of psoriatic patients,and probe into the relationship between the colony formation and P16 promoter mRNA expression. Methods:Bone marrow-derived mononuclear cells from the psoriatics and normal controls were purified by density gradient centrifugation. The bone marrow mononuclear cells were cultured in methylcellulose semi-solid culture medium with SCF,GM-CSF,IL-3 and IL-6 for 14 days. The colony cells were collected and the mRNA expression of P16 gene in colony cells was detected by using reverse transcription-polymerase chain reaction (RT-PCR). Results:In methylcellulose semi-solid culture system, the number and size of the colonies of HPP-CFC of psoriatic bone marrow were significantly less than those of the normal control with higher positive frequency of P16 gene mRNA expression compared with the normal control. Conclusion:The higher positive frequency of P16 gene mRNA expression in HPP-CFC may play a role in reducing HPP-CFC colony-forming capability of psoriatic hematopoietic cells.

目的:研究银屑病患者骨髓高增殖潜能集落形成细胞(HPP-CFC)的集落形成能力及P16基因mRNA表达,探讨银屑病患者骨髓造血干细胞体外增殖活性及原因。方法:密度梯度离心法分离银屑病患者及正常对照骨髓单个核细胞,培养于含人干细胞因子(SCF)、人粒-巨噬细胞系集落剌激因子(GM-CSF)、白介素(IL)-3、IL-6细胞因子组合的甲基纤维素半固体培养基,培养14d时计数HPP-CFC集落,然后收集集落。提取纯化集落细胞的总RNA,应用反转录(RT)-PCR方法检测HPP-CFC集落细胞的P16基因mRNA的表达。结果:①在甲基纤维素半固体培养基中,银屑病患者骨髓HPP-CFC集落数显著低于正常对照组,且集落形态较小;②银屑病患者骨髓HPP-CFC集落细胞的P16基因mRNA表达高于正常对照组,差异有统计学意义。结论:银屑病患者骨髓HPP-CFC集落形成能力降低;银屑病患者骨髓HPP-CFC的P16基因mRNA表达阳性率增高,推测P16基因可能参与了银屑病患者骨髓造血干细胞体外增殖活性异常的发生。

 
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