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clamping injury
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  夹伤
     Eighty adult SD rats were divided into transfected SCs treating group (group A),untransfected SCs treating group (group B),DMEM treating group (group C) and operating control group (group D),and 20 rats in every group. The right eye of every rat was applied to establish optic nerve clamping injury model,and the left eye of every rat in group D was applied to blank group (group E).
     80只成年SD大鼠随机分为转染有BDNF基因的SCs治疗组(A组)、正常SCs治疗组(B组)、DMEM治疗组(C组)和手术对照组(D组),每组20只,每只右眼建立视神经夹伤模型,D组大鼠左眼作为空白对照组(E组)。
短句来源
     Retinal ganglial cells (RGCs) of all rats were labeled with fluorogold (FG) in the 7th day before clamping injury.
     夹伤前7d进行荧光金(fluorogold,FG)逆行标记视网膜神经节细胞(retinalganglialcells,RGCs)。
短句来源
     Except of group D and E,10 μL of corresponding treatment liquid was instantly injected into the vitreous of injuryed eyes respectively after clamping injury.
     夹伤后即刻向A、B、C组伤眼玻璃体腔内注入相应液体各10μL。
短句来源
     The ratio of P1 wave amplitude in group A showed downtrend along with time,but it was evidently higher than other groups on the 14th,21th and 28th day after clamping injury (P<0.05).
     A组的P1波幅比随观察时间延长呈下降趋势,但在夹伤后第14d、21d、28d时仍明显高于其他各组(P<0.05)。
短句来源
     Conclusion By administering intraocularly,the cultured SCs which were transfected with human BDNF gene can promote the repair axons of RGCs,increase the survival rate of RGCs and dramatically promote the function of the optic nerve after clamping injury.
     结论视神经夹伤后,玻璃体腔内注射转染有BDNF基因的SCs能够促进RGCs轴突的修复,提高RGCs存活率,保护视神经功能,对视神经损伤修复具有明显的保护作用。
短句来源
  “clamping injury”译为未确定词的双语例句
     Changes of nNOS immuno-positive neuron numbers of rat's spinal ganglion and spinal cord following radial nerve clamping injury
     大鼠桡神经钳夹伤后背根节和脊髓nNOS免疫阳性神经元的变化
短句来源
     On the 7th,14th,21th and 28th day after clamping injury, flash visual evoked potentials (FVEP) tests at various intervals and number of RGCs counting were performed.
     分别在伤后第7d、14d、21d、28d时进行闪光视觉诱发电位(flashvisualevokedpotentials,FVEP)检测和全视网膜铺片、记数RGCs。
短句来源
  相似匹配句对
     Injury;
     外伤;
短句来源
     Lung Injury Induced by Aortic Cross-clamping and Protection of Propofol
     主动脉阻断引起的肺损伤和丙泊酚的保护作用
短句来源
     In the injury theory of R.
     AS的发病机制尚不明确,R.
短句来源
     Methods: IIR injury was induced by clamping and unclamping of superior mesenteric artery.
     方法:通过肠系膜上动脉钳闭——松开造成猕猴肠缺血再灌注损伤;
短句来源
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Objective To study the protective effects of the cultured Schwann cells (SCs),which origined from Sprague-Dawley (SD) rats and transfected with human brain-derived neurotrophic factor (BDNF) gene mediated by recombinant adenovirus in vitro,on repair of optic nerve injury.Methods Human BDNF gene was transfected into the cultured SCs in vitro,and then the expression of BDNF in the cell-cultured supernatants was tested by enzyma-linked immumosorbent assay (ELISA).Eighty adult SD rats were divided into transfected...

Objective To study the protective effects of the cultured Schwann cells (SCs),which origined from Sprague-Dawley (SD) rats and transfected with human brain-derived neurotrophic factor (BDNF) gene mediated by recombinant adenovirus in vitro,on repair of optic nerve injury.Methods Human BDNF gene was transfected into the cultured SCs in vitro,and then the expression of BDNF in the cell-cultured supernatants was tested by enzyma-linked immumosorbent assay (ELISA).Eighty adult SD rats were divided into transfected SCs treating group (group A),untransfected SCs treating group (group B),DMEM treating group (group C) and operating control group (group D),and 20 rats in every group.The right eye of every rat was applied to establish optic nerve clamping injury model,and the left eye of every rat in group D was applied to blank group (group E).Retinal ganglial cells (RGCs) of all rats were labeled with fluorogold (FG) in the 7th day before clamping injury.Except of group D and E,10 μL of corresponding treatment liquid was instantly injected into the vitreous of injuryed eyes respectively after clamping injury.On the 7th,14th,21th and 28th day after clamping injury, flash visual evoked potentials (FVEP) tests at various intervals and number of RGCs counting were performed. Results The quantity of expression of BDNF protein in transfected SCs evidently increased than untransfected SCs.The ratio of P1 wave amplitude in group A showed downtrend along with time,but it was evidently higher than other groups on the 14th,21th and 28th day after clamping injury (P<0.05).On the 21th and 28th day,number of RGCs in group A were (1 591±82)·mm -2 and (1 516±91)·mm -2,which evidently higher than other groups except of group E (P<0.01).Conclusion By administering intraocularly,the cultured SCs which were transfected with human BDNF gene can promote the repair axons of RGCs,increase the survival rate of RGCs and dramatically promote the function of the optic nerve after clamping injury.

目的研究经腺病毒介导,转染有人脑源性神经营养因子(brain-derivedneu-rotrophicfactor,BDNF)基因的SD大鼠雪旺细胞(Schwanncells,SCs)对成年SD大鼠视神经夹伤后修复的保护作用。方法将人BDNF基因转染到体外培养的SCs内,采用酶联免疫反应(enzyma-linkedimmumosorbentassay,ELISA)检测培养液上清中BDNF的表达量。80只成年SD大鼠随机分为转染有BDNF基因的SCs治疗组(A组)、正常SCs治疗组(B组)、DMEM治疗组(C组)和手术对照组(D组),每组20只,每只右眼建立视神经夹伤模型,D组大鼠左眼作为空白对照组(E组)。夹伤前7d进行荧光金(fluorogold,FG)逆行标记视网膜神经节细胞(retinalganglialcells,RGCs)。夹伤后即刻向A、B、C组伤眼玻璃体腔内注入相应液体各10μL。分别在伤后第7d、14d、21d、28d时进行闪光视觉诱发电位(flashvisualevokedpotentials,FVEP)检测和全视网膜铺片、记数RGCs。结果转染有BDNF基因的SCs的BDNF表达量明显高于...

目的研究经腺病毒介导,转染有人脑源性神经营养因子(brain-derivedneu-rotrophicfactor,BDNF)基因的SD大鼠雪旺细胞(Schwanncells,SCs)对成年SD大鼠视神经夹伤后修复的保护作用。方法将人BDNF基因转染到体外培养的SCs内,采用酶联免疫反应(enzyma-linkedimmumosorbentassay,ELISA)检测培养液上清中BDNF的表达量。80只成年SD大鼠随机分为转染有BDNF基因的SCs治疗组(A组)、正常SCs治疗组(B组)、DMEM治疗组(C组)和手术对照组(D组),每组20只,每只右眼建立视神经夹伤模型,D组大鼠左眼作为空白对照组(E组)。夹伤前7d进行荧光金(fluorogold,FG)逆行标记视网膜神经节细胞(retinalganglialcells,RGCs)。夹伤后即刻向A、B、C组伤眼玻璃体腔内注入相应液体各10μL。分别在伤后第7d、14d、21d、28d时进行闪光视觉诱发电位(flashvisualevokedpotentials,FVEP)检测和全视网膜铺片、记数RGCs。结果转染有BDNF基因的SCs的BDNF表达量明显高于正常SCs。A组的P1波幅比随观察时间延长呈下降趋势,但在夹伤后第14d、21d、28d时仍明显高于其他各组(P<0.05)。夹伤后第21d和第28d时,A组RGCs数分别为(1591±82)·mm-2、(1516±91)·mm-2,明显高于除E组外的其他各组(P<0·01)。结论视神经夹伤后,玻璃体腔内注射转染有BDNF基因的SCs能够促进RGCs轴突的修复,提高RGCs存活率,保护视神经功能,对视神经损伤修复具有明显的保护作用。

Objective To study different types of iatrogenic injury to the cervical segment of recurrent laryngeal nerve (RLN) in rabbits. Methods Twenty New Zealand rabbits were randomly divided into four groups according to different types of injury to the recurrent laryngeal nerve. Four common types of iatrogenic injury in cervical segment of RLN in thyroid gland surgery (TGS), i.e stretching, ligation, clamping, and electrocautery, were reproduced in the animals. The compound muscle action potential (CMAP) was recorded...

Objective To study different types of iatrogenic injury to the cervical segment of recurrent laryngeal nerve (RLN) in rabbits. Methods Twenty New Zealand rabbits were randomly divided into four groups according to different types of injury to the recurrent laryngeal nerve. Four common types of iatrogenic injury in cervical segment of RLN in thyroid gland surgery (TGS), i.e stretching, ligation, clamping, and electrocautery, were reproduced in the animals. The compound muscle action potential (CMAP) was recorded by means of nerve integrity monitor (NEM) with bipolar electrodes inserted into the relevant muscles. The minimal stimulus current intensity threshold (MSCIT) of evoked electromyography (EMG) in laryngeal muscles was obtained after injury of RLN. The exposed RLN before injury was used as self control. Results The average MSCIT of RLNs in rabbits before injury was 0.05mA. The average MSCIT of damaged groups (stretch, ligation, clamping and electrocautery group) was 0.24mA, 1.04mA, ≥3.0mA and 2.81mA, respectively. There was no significant difference in MSCIT between clamping injury and control, also between clamping and electrocautery (P>0.05). But the diffence was significant in other groups (P<0.05). Conclusion Clamping and electrocautery injury to the nerve gives rise to obvious impairment of the function of RLN (P<0.05). As there is no significant difference in MSCIT between stretching group and control, it seems that. RNL can resist stretching to some extent.

目的研究不同类型医源性损伤对家兔颈段喉返神经(RLN)功能的影响。方法将20只新西兰白兔随机分为4组(牵拉组、结扎组、电凝组、钳夹组),模拟临床甲状腺手术中可能发生的颈段RLN常见损伤,建立4种医源性RLN损伤的动物模型,应用运动神经功能监护仪经插入式双极电极采集复合肌肉动作电位(CMAP),获取不同类型损伤后诱发喉肌电图的最小刺激电流强度阈值(MSCIT)。采用自身对照方法,与自身损伤前结果比较。结果新西兰兔RLN损伤前的平均MSCIT为0·05mA,损伤后各组(牵拉组、结扎组、电凝组、钳夹组)的平均MSCIT分别为0·24mA、1·04mA、≥3·0mA和2·81mA,牵拉组与对照组比较,钳夹组与电凝组比较,无显著差异(P>0·05),其余各组间有显著差异(P<0·05)。结论电凝和钳夹对神经功能的影响最为显著,结扎和牵拉的影响相对较小,RLN具有一定的抗牵拉作用。

 
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