助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   osteogenesis capability 的翻译结果: 查询用时:0.197秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

osteogenesis capability
相关语句
  成骨性能
     Objective: To study the osteogenesis capability of prefabricated-mineralized inducement scaffold material by reconstructing the large-scale chest-like defect in the mandible cornu of the experimental goat, and the application value of the material in the bone tissue engineering.
     目的:应用无机诱导因子复合性支架材料修复山羊的下颌骨角部大型箱状缺损,研究该材料的成骨性能,探讨其在骨组织工程中的应用价值。
短句来源
  “osteogenesis capability”译为未确定词的双语例句
     The Construction of Two Kinds of Artificial Bone and the Comparison of Their Osteogenesis Capability in vivo
     两种人工骨的构建及其在体内诱导成骨能力的比较
短句来源
     Construction of Three Kinds of Membrane Guided Bone Regeneration and Comparison of Their Osteogenesis Capability in Vivo
     三种诱导骨再生膜的构建及其诱导成骨能力的比较
短句来源
     Objectives To compare the neovascularization and osteogenesis capability of rhBMP-2/DFDBA complex and DFDBA at various sites in rats.
     目的 从血管再生的角度,比较rhBMP-2/DFDBA复合材料及单纯DFDBA材料在血管+肌,肌,皮下和脂肪组织四种部位异位植入后的成骨能力及血管再生能力。
短句来源
     Conclusion Since cryopreservation does not affect the growth and osteogenesis capability of BMSCs on DBM,the cryopreserved BMSCs can be used as a cell source in bone tissue engineering.
     结论低温保存对人BMSCs在DBM上的体外增殖、粘附及成骨能力影响差异无显著性意义,低温保存的人BMSCs可作为组织工程骨的种子细胞。
短句来源
  相似匹配句对
     After induced by osteogenesis supplement, BMSCs exhibited osteogeneric capability.
     传代细胞经成骨添加剂诱导后,具有较强的碱性磷酸酶活性,并能表达Ⅰ型胶原蛋白,呈现成骨细胞活性;
短句来源
     On the Capability in Innovation
     论创新能力
短句来源
     NAT has the capability.
     本文重点研究利用NAT和IPsec技术进行防火墙设计。
短句来源
     The Construction of Two Kinds of Artificial Bone and the Comparison of Their Osteogenesis Capability in vivo
     两种人工骨的构建及其在体内诱导成骨能力的比较
短句来源
     The Distraction Osteogenesis for Jawbone
     颌骨牵引成骨技术
短句来源
查询“osteogenesis capability”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


Objective To seek the best way of isolating and culturing bone marrow mesenchymal stem cells (BMMSCs).Histomorphology,ultrastructure,kinetics,expression of ALP and,calcified deposition in vitro were also studied.Methods Bone marrow was collected from femur and tibia of 2 month old male SD rats and seeded in cell culture flask.Their morphology and ultrastructure were analyzed by phase contrast microscope and transmission electron microscope (TEM).Alkaline phosphatase histochemical staining was performed with...

Objective To seek the best way of isolating and culturing bone marrow mesenchymal stem cells (BMMSCs).Histomorphology,ultrastructure,kinetics,expression of ALP and,calcified deposition in vitro were also studied.Methods Bone marrow was collected from femur and tibia of 2 month old male SD rats and seeded in cell culture flask.Their morphology and ultrastructure were analyzed by phase contrast microscope and transmission electron microscope (TEM).Alkaline phosphatase histochemical staining was performed with Gomarri method.Calcified deposition was checked with modified VonKossa method.Results BMMSCs had spindle shape,with irregular processes on cell surface.Large number of rough surfaced endoplasmic reticuli and mitochondria were observed in cells through TEM.Cell doubling time was 72 hours.Induced by culture medium containing dexamethasone,β-glycerphosphate sodium and ascorbic acid,BMMSCs could express ALP obviously.Calcified deposition,which was one characteristics of osteoblast,could also be seen in vitro.Conclusion BMMSCs culture system is established.Through induction,they can differntiate to cells with osteogenesis capability,and may be used to provide cells for bone tissue engineering.

目的 建立大鼠骨髓间质干细胞 (bonemarrowmesenchymalstemcells ,BMMSCs)体外培养体系。对大白鼠BMMSCs体外培养的条件、供体大白鼠鼠龄等进行了研究。对体外培养的大白鼠BMMSCs的形态学、增殖动力学、成骨的表面标记及体外钙化能力进行了系列研究。方法 取 2月龄SD大鼠胫骨和股骨骨髓进行体外培养。取第二代BMMSCs,绘制生长曲线并计算群体倍增时间。对经成骨诱导剂诱导的BMMSCs的ALP表达及体外钙沉积进行了研究。结果 细胞呈长梭形或多角形 ,细胞表面有不规则的细胞突起。透射电镜可见胞浆有大量的粗面内质网及线粒体 ,细胞表面可见突起。细胞生长曲线呈S形 ,群体倍增时间为 72小时。经诱导分化的BMMSCs可见碱性磷酸酶的表达。vonKossa染色可见散在深染的钙沉积斑块。结论 来源于年轻大鼠股骨骨髓的间质干细胞经体外培养并经诱导后可分化为具有部分成骨特性的细胞 ,可以为体内骨组织工程提供组织细胞。

Objective: To study the osteogenesis capability of prefabricated-mineralized inducement scaffold material by reconstructing the large-scale chest-like defect in the mandible cornu of the experimental goat, and the application value of the material in the bone tissue engineering. Methods: 15 goats were operated on both sides of mandible cornu to prepare the large-scale chest-like defects of 30 mm×25 mm×10 mm, with prefabricated-mineralized inducement scaffold material put into the left side(group A), and...

Objective: To study the osteogenesis capability of prefabricated-mineralized inducement scaffold material by reconstructing the large-scale chest-like defect in the mandible cornu of the experimental goat, and the application value of the material in the bone tissue engineering. Methods: 15 goats were operated on both sides of mandible cornu to prepare the large-scale chest-like defects of 30 mm×25 mm×10 mm, with prefabricated-mineralized inducement scaffold material put into the left side(group A), and blank control on the right side (group B). These goats were divided into three groups according to the three time points of 4, 8, 12 weeks. After the operation, the animals were executed 4, 8, 12 weeks respectively. The defect restoration areas and the material-bone interface samples were taken to observe tissue dynamics. Results: 4~12 weeks later, bone defects of group A had the process of progressing osteogenesis, calcification and bone marrow formation. The bone defects of group B were fiber connective tissue, whose inferior margins were immature chondrocyte attachment. Conclusion: Prefabricated-mineralized inducement scaffold material, with quite good bioactivity and bone reconstructed ability, has the feasibility to clinical application.

目的:应用无机诱导因子复合性支架材料修复山羊的下颌骨角部大型箱状缺损,研究该材料的成骨性能,探讨其在骨组织工程中的应用价值。方法:山羊15只,按4、8、12周3个时间点分为3组,于双侧下颌角手术制备30mm×25mm×10mm的大型箱状缺损,采用自身配对设计,左侧置入支架材料(A组),右侧空白对照(B组)。术后4、8、12周分别处死动物,取缺损修复区和材料—骨界面标本,观察组织动态学。结果:!4~12周A组有进行性成骨、钙化、骨髓形成的过程,而B组骨缺损内为未充满的纤维结缔组织,下缘有不成熟的软骨细胞附着。结论:支架材料有相当良好的生物活性;修复骨缺损显示优良的修复效果;具有临床应用的可行性。

Objective To investigate the effects of cryopreservation on the growth and osteogenesis capa- bility of human bone marrow stromal cells(BMSCs)on demineralized bene matrix(DBM).Methods Bone marrow aspirates were obtained from the lilac crests of three donors.The BMSCs were isolated from the bone marrow by density gradient centrifugation.Cells of passage 3 were cryopreserved in liquid nitrogen for 24 hours,and then re- covered.The non-cryopreserved BMSCs were used as the control,The cryopreserved and control BMSCs...

Objective To investigate the effects of cryopreservation on the growth and osteogenesis capa- bility of human bone marrow stromal cells(BMSCs)on demineralized bene matrix(DBM).Methods Bone marrow aspirates were obtained from the lilac crests of three donors.The BMSCs were isolated from the bone marrow by density gradient centrifugation.Cells of passage 3 were cryopreserved in liquid nitrogen for 24 hours,and then re- covered.The non-cryopreserved BMSCs were used as the control,The cryopreserved and control BMSCs were cul- tured in osteogenic media,collected and labeled with Dil to be seeded onto the DBM when cells were confluent.The percentage of BMSCs adhered to the DMB was detected.The cell morphology and matrices secreted by BMSCs on the DBM were observed by the inverted phase-contrasted microscope,fluorescence microscope and scanning electron microscope(SEM).The growth and viability of BMSCs on the DBM were determined using the modified MTT ashy. The osteogenesis ability of BMSCs on the DBM was determined by assessment of the alkaline phosphatase(ALP) activity and osteocalcin(OCN)content.Results The percentages of the cryopreserved and control cells adhered to DBM were(97.25±1.17)% and(97.00±1.09)% respectively.The cells adhered well to the DBM and grew rapidly.Large amounts of matrices on the DBM were observed by the light microscope and SEM.The cells embedded in the matrices could be observed by fluorescence microscope.There were no significant differences in the assay values of MTT,ALP and OCN between the cryopreserved and control BMSCs on the DBM.Conclusion Since cryopreservation does not affect the growth and osteogenesis capability of BMSCs on DBM,the cryopreserved BMSCs can be used as a cell source in bone tissue engineering.

目的研究低温保存对人骨髓基质干细胞(BMSCs)在脱钙骨(DBM)上生长特性及成骨能力的影响。方法取3例志愿者骨髓(3~5mL),密度梯度离心、差速贴壁法获得BMSCs。第3代BMSCs在-196℃下保存24h,37℃复苏,测定细胞成活率。低温保存前、后的BMSCs分别用成骨诱导液诱导培养,至90%融合时,收集细胞接种在DBM支架上,并测定细胞在DBM上的粘附率。DiI荧光染料标记BMSCs,例置相差显微镜、荧光显微镜和SEM观察低温保存前、后的BMSCs在DBM上的生长及基质分泌情况,MTT法测定细胞在DBM上的增殖活性。通过测定碱性磷酸酶(ALP)活性和骨钙素(OCN)含量观察细胞在DBM上的成骨能力。结果复苏细胞的存活率为(90.24±0.02)%。低温保存前、后的BMSCs在DBM上的粘附率分别为(97.25±1.17)%和(97.00±1.09)%。倒置相差显微镜及SEM观察显示低温保存前、后的BMSCs在DBM上粘附、生长良好,有大量细胞外基质分泌、沉积。低温保存前、后的BMSCs在DBM上MTT吸光度值和ALP活性的检测结果差异无显著性意义(P>0.05);体外培养12d时,MTT吸光度值及AL...

目的研究低温保存对人骨髓基质干细胞(BMSCs)在脱钙骨(DBM)上生长特性及成骨能力的影响。方法取3例志愿者骨髓(3~5mL),密度梯度离心、差速贴壁法获得BMSCs。第3代BMSCs在-196℃下保存24h,37℃复苏,测定细胞成活率。低温保存前、后的BMSCs分别用成骨诱导液诱导培养,至90%融合时,收集细胞接种在DBM支架上,并测定细胞在DBM上的粘附率。DiI荧光染料标记BMSCs,例置相差显微镜、荧光显微镜和SEM观察低温保存前、后的BMSCs在DBM上的生长及基质分泌情况,MTT法测定细胞在DBM上的增殖活性。通过测定碱性磷酸酶(ALP)活性和骨钙素(OCN)含量观察细胞在DBM上的成骨能力。结果复苏细胞的存活率为(90.24±0.02)%。低温保存前、后的BMSCs在DBM上的粘附率分别为(97.25±1.17)%和(97.00±1.09)%。倒置相差显微镜及SEM观察显示低温保存前、后的BMSCs在DBM上粘附、生长良好,有大量细胞外基质分泌、沉积。低温保存前、后的BMSCs在DBM上MTT吸光度值和ALP活性的检测结果差异无显著性意义(P>0.05);体外培养12d时,MTT吸光度值及ALP活性同时达到峰值。低温保存前、后的BMSCs在DBM上分泌OCN的量差异无显著性意义(P>0.05),OCN含量随着培养时间延长而不断上升,在观察期(16d)内未出现平台期。结论低温保存对人BMSCs在DBM上的体外增殖、粘附及成骨能力影响差异无显著性意义,低温保存的人BMSCs可作为组织工程骨的种子细胞。

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关osteogenesis capability的内容
在知识搜索中查有关osteogenesis capability的内容
在数字搜索中查有关osteogenesis capability的内容
在概念知识元中查有关osteogenesis capability的内容
在学术趋势中查有关osteogenesis capability的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社