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polyclonal serum
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  多克隆血清
     The results showed that the fusion protein was about 48 kDa and it had special combination activity with anti-LCDV polyclonal serum.
     结果表明,该融合蛋白分子量约48kD,可与抗LCDV多克隆血清特异反应。
短句来源
     The results showed that the fusion protein was about 70 kD and it had special combination activity with anti-LCDV polyclonal serum.
     结果表明,该融合蛋白分子量约70kD,可与抗LCDV多克隆血清特异反应。
短句来源
     Detection of western blot using ant-AmPLA2 polyclonal serum of rabbit as the first antibody showed that a special blot with the some characteristics as the native AmPLA2 was revealed among the expression products. This was considered as the evidence for successful expression of AmPLA2 in E. coli.
     用兔抗AmPLA2多克隆血清为第一抗体作Westernblot检测,表达产物显示与天然AmPLA2同样的特异性印迹,证实AmPLA2基因已在大肠杆菌中得到表达.
短句来源
  “polyclonal serum”译为未确定词的双语例句
     Western blot analysis using anti-AmPLA_2 polyclonal serum confirmed the expressed protein was a fusion protein of AmPLA_2. The protein extracts of AmPLA_2 showed an enzymatic activity of about 6.13 μmol·min~ -1 ·mg~ -1 for hydrolyzing egg yolk substrate.
     Westernblot印迹显示,融合表达产物能与意大利蜜蜂蜂毒AmPLA2抗血清发生免疫反应。 生物活性测定显示,含表达产物的细胞蛋白粗提物对底物蛋黄的酶活力约为6·13μmol·min-1·mg-1。
短句来源
     5. We also used the washed inclusion body to immunolize rabbit to prepare polyclonal serum of GST-STS . Western blotting shows that the serum (diluted to 1:3000) had specific antigen-antibody recognition to the renatured GST-STS fusion protein .
     4. GST-STS 融合蛋白主要以包涵体形式表达,通过高浓度变性剂溶解包涵体和低浓度变性剂梯度透析对该包涵体进行了复性。
短句来源
     Detection of western blot using ant-European honeybee (Apis mellifera) phospholipase A2 (AmPLA2) polyclonal serum as the first antibody showed that the expression products appeared a special blot same as the native AmPLA2.The result demonstrated that the AcPLA2 peptide had been expressed in E. coli and the AcPLA2 has the similar antigenicity as the AmPLA2.
     用意大利蜜蜂蜂毒磷脂酶A2(AmPLA2)纯品制备的兔源多克隆抗体为一抗作Western blot,表达产物显示类似于天然纯AmPLA2的特异性印迹,证实AcPLA2基因已在大肠杆菌中得到表达。
短句来源
     In the haemagglutination test, the recombinant VLPs, like the native RHDV, also agglutinated human blood type O erythrocytes and could be inhibited by the anti_RHDV polyclonal serum.
     该病毒样颗粒与兔抗RHDV高免血清作用后可被凝集成团 ,表明该VLPs与天然RHDV在抗原性上也极为相似。
短句来源
     The (His)6-tagged GGNBP1 was purified by Ni2+-charged HiTrap Chelating HP column, then coupled to a NHS-activated HiTrap column as an antigen column to immunoaffinity-purify specific anti-GGNBP1 antibody from the polyclonal serum.
     镍离子金属螯合柱纯化表达的GGNBP1蛋白,与NHS活化基团交联,制备GGNBP1抗体亲和层析柱,纯化GGNBP1多抗。
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  相似匹配句对
     The serum C.
     ELISA法检测血清C .
短句来源
     Results The polyclonal antibodies were examined in the animals' serum.
     结果 在动物血清中检测到多克隆抗体。
短句来源
     Preparation of the polyclonal antibodies (PcAb) from rabbit serum
     制备兔多抗血清
短句来源
     The serum Vit.
     观察治疗前后ALT、胆红素 (Bil)、血清Vit.
短句来源
     Production of Polyclonal Antibody of NFLX
     诺氟沙星多克隆抗体的制备
短句来源
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  polyclonal serum
However, anti-gD monoclonal antibody and anti-PRV polyclonal serum failed to neutralize gD-expressing recombinants.
      
Anti-PRV gB and gC antibodies as well as anti-PRV polyclonal serum neutralized BHV-1 recombinants which express PRV gB and gC.
      
Expressed σC fusion protein in Escherichia coli BL21 strain could be detected by Western blotting under duck anti-reovirus polyclonal serum.
      
Expression analysis of the chosen clone was confirmed by western blotting with F protein specific polyclonal serum.
      
Immunoelectronmicroscopical studies revealed the presence of ANF in the ASGs of pigs and cattle, whereas anti-ANF polyclonal serum failed to detect any significative reaction in lapine ASGs.
      
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A fluorometric assay was used to confirm enzyme activity in chitinase gene transferred tobacco.Chitinase gene activity in transgene tobacco is higher than that in untransgene counterparts.Confocal laser scanning microscopy of foliar sections from a chitinase expressing Nicotiana tabacum Xanthinc embedded in (butylmethyl methacrylate) and stained with a polyclonal serum raised against the chitinase/fluorconjugated secondary(IgG) antibody detection system showed that chitinase was largely restricted...

A fluorometric assay was used to confirm enzyme activity in chitinase gene transferred tobacco.Chitinase gene activity in transgene tobacco is higher than that in untransgene counterparts.Confocal laser scanning microscopy of foliar sections from a chitinase expressing Nicotiana tabacum Xanthinc embedded in (butylmethyl methacrylate) and stained with a polyclonal serum raised against the chitinase/fluorconjugated secondary(IgG) antibody detection system showed that chitinase was largely restricted to the vascular tissue,with a few accumulating in chloroplasts.Among the transgenic tobacco T2 plants inoculated with Colletotrichum nicotianae Averna,several lines displayed resistance to the fungus.

采用荧光定量法对转几丁质酶基因烟草T2植株的几丁质酶活性进行了测定,结果表明,转基因烟草株系的几丁质酶活性明显高于未转基因烟草,同时发现,pH对转基因烟草中的几丁质酶活性有一定的影响。对转基因烟草接种烟草炭疽病菌进行抗病性鉴定。

Paralichthys olivaceus gilled cells FG-9307 was infected by lymphocystis disease virus. The total cell RNA was extracted from it by using Trizol Reagent. LCDV major capsid protein 0.6kb gene fragment was obtained by RT-PCR. Then the prokaryotic expression vector pGEX-4Tl-LCDV0. 6 was constructed.. After inducing expressed the fusion protein by IPTG, Western blotting was used to identify the activity of the protein. The results showed that the fusion protein was about 48 kDa and it had special combination activity...

Paralichthys olivaceus gilled cells FG-9307 was infected by lymphocystis disease virus. The total cell RNA was extracted from it by using Trizol Reagent. LCDV major capsid protein 0.6kb gene fragment was obtained by RT-PCR. Then the prokaryotic expression vector pGEX-4Tl-LCDV0. 6 was constructed.. After inducing expressed the fusion protein by IPTG, Western blotting was used to identify the activity of the protein. The results showed that the fusion protein was about 48 kDa and it had special combination activity with anti-LCDV polyclonal serum. It established foundation to research the genetic engineering vaccine of LCDV.

淋巴囊肿病病毒感染牙鲆鳃细胞系FG-9307,提取细胞总RNA,采用RT-PCR法成功获得了淋巴囊肿病病毒主要衣壳蛋白0.6kb基因片段。构建其原核表达载体,IPTG诱导表达。结果表明,该融合蛋白分子量约48kD,可与抗LCDV多克隆血清特异反应。为LCDV基因工程疫苗的研制奠定了实验基础。

The venomous phospholipase A2 (AmPLA2) coding reading region of Italian honeybee (Apis mellifera), which was found to be composed of 405 bp, was inserted into the expression vector pETblue-1. Then the recombinant vector was introduced into Escherichia coli Tuner (DE3) placⅠfor expression. Analysis of the result of SDS-PAGE showed that the expression products contained a band of protein of about 15 kD. Detection of western blot using ant-AmPLA2 polyclonal serum of rabbit as the first antibody showed...

The venomous phospholipase A2 (AmPLA2) coding reading region of Italian honeybee (Apis mellifera), which was found to be composed of 405 bp, was inserted into the expression vector pETblue-1. Then the recombinant vector was introduced into Escherichia coli Tuner (DE3) placⅠfor expression. Analysis of the result of SDS-PAGE showed that the expression products contained a band of protein of about 15 kD. Detection of western blot using ant-AmPLA2 polyclonal serum of rabbit as the first antibody showed that a special blot with the some characteristics as the native AmPLA2 was revealed among the expression products. This was considered as the evidence for successful expression of AmPLA2 in E. coli.

将意大利蜜蜂蜂毒磷脂酶A2(AmPLA2)成熟肽编码区基因(405bp)插入表达载体pETBlue-1,在大肠杆菌Tuner(DE3)plac 中诱导表达,经SDS-PAGE电泳检测,表达产物分子量为15kD,约占细菌总蛋白的4.3%;用兔抗AmPLA2多克隆血清为第一抗体作Westernblot检测,表达产物显示与天然AmPLA2同样的特异性印迹,证实AmPLA2基因已在大肠杆菌中得到表达.

 
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