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immunological binding
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  “immunological binding”译为未确定词的双语例句
     coli BL21 Star. After induction with IPTG,the expressed Bla g2 protein was purified through metal(Ni~(2+)) chelating affinity chromatography. Western blotting was used to determine the immunological binding activity of the recombinant allergen.
     挑取重组子,用NdeⅠ和NotⅡ双酶切后将目的片段亚克隆入pET24a(+),经IPTG诱导表达后,通过Ni2+亲和层析柱对重组变应原进行纯化,并采用Western-blot检测其免疫学结合活性。
短句来源
  相似匹配句对
     protein binding;
     蛋白结合;
短句来源
     THE IMMUNOLOGICAL PROPERTY OF CALCIUM BINDING PROTEIN CaBP_(72)
     钙结合蛋白CaBP_(72)免疫特性的研究
短句来源
     THE IMMUNOLOGICAL ACTIVITY AND RECEPTOR BINDING ABILITY OF DESHEPTAPEPTIDE (B24-30) INSULIN
     去B链羧端七肽胰岛素的免疫活性及受体结合能力
短句来源
     Binding of TNFs with OPG
     人OPG与肿瘤坏死因子族蛋白的结合研究
短句来源
     Immunological adjuvant and chitosan
     免疫佐剂与壳聚糖
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  immunological binding
Since the amount of antitoxin bound to muscle or brain was greatly increased by prior incubation of the sections with toxin it follows that this is an example of specific immunological binding.
      
False-positive results may be observed due to non-immunological binding of proteins or substrate reaction products.
      
False-positive results may be seen due to non-immunological binding of proteins or substrate reaction products.
      
False positive results may be seen because of non immunological binding of proteins or substrate reaction products.
      
False positive results may be seen due to non-immunological binding of proteins or substrate reaction products.
      


A new fluorescent method for testing the binding capacities of different cells was established. The target cells were labeled with non - toxic vital fluorescence dye,Calcein AM, then cocultured with human monocytes activated by rhIFN - 7 and IPS for 4hrs. The binding capacity was assayed with Cyto Fluor 2300 before and after removing unbinding target cells. The results showed that activated human monocytes could bind actively to the target cells especially tumorigenic cells. The expression of cellular adhesion...

A new fluorescent method for testing the binding capacities of different cells was established. The target cells were labeled with non - toxic vital fluorescence dye,Calcein AM, then cocultured with human monocytes activated by rhIFN - 7 and IPS for 4hrs. The binding capacity was assayed with Cyto Fluor 2300 before and after removing unbinding target cells. The results showed that activated human monocytes could bind actively to the target cells especially tumorigenic cells. The expression of cellular adhesion moleculars (CAMs),especially CD 11/CD 18 on the human monocytes was dramatically increased after activated by LPS and rhIFN - γ. Blocking of CAMs with monoclonal antibodies would inhibit the binding capacities between the activated monocytes and tumorigenic target cells. The inhibitor of protein kinases, H - 7, also showed some inhibition to the binding capacities of these two kinds of cells. The binding capacities were also increased when target cells were pretreated with rhIFN - γ and LPS. These results indicated that integrins and their ligands are of very important functions in the immunological binding and recognition.

本文报道用无毒性的活体荧光染料—钙黄绿素 AM标记的肿瘤细胞与人单核细胞共培养4小时,用Cyto Fluo 2300测定细胞粘附能力的荧光检测技术.结果表明IFN-γ和LPS激活的人单核细胞能迅速地与悬浮生长的人肿瘤细胞、B淋巴母细胞及淋巴细胞粘附.激活的人单核细胞表达更多的CD11a、CD11b、CD11c和CD18,对肿瘤细胞有更多的粘附性.抗体封闭及蛋白激酶抑制物处理细胞,粘附能力均受到明显的抑制.IFN-γ和LPS处理肿瘤细胞也能明显增加两种细胞间的粘附能力.上述结果提示人单核细胞粘附能力的增加与整合素及肿瘤细胞所表达的相关配体增加有关.

Aim To detect the biological activity of the home made recombinant human angiogenin and its monoclonal antibody(mAb). Methods Gel scanning was used to analyze the purity of home made rhANG. ELISA and chicken CAM assay were used to detect the specificity and biological activity of ANG and anti ANG mAb, respectively. Immunobinding activities of home made rhANG and the standard rhANG were compared by routine ELISA. Results The purity of home made rhANG reached 96% and could induce the angiogenesis. The anti...

Aim To detect the biological activity of the home made recombinant human angiogenin and its monoclonal antibody(mAb). Methods Gel scanning was used to analyze the purity of home made rhANG. ELISA and chicken CAM assay were used to detect the specificity and biological activity of ANG and anti ANG mAb, respectively. Immunobinding activities of home made rhANG and the standard rhANG were compared by routine ELISA. Results The purity of home made rhANG reached 96% and could induce the angiogenesis. The anti ANG mAb showed high specificity and inhibited effectively the proliferation of blood vessels induced by angiogenin. The home made and standard ANG, both products possessed good immunological binding activity. Conclusion The home made rhANG and its mAb can be used for further basic and clinical research.

目的鉴定自制重组人血管生长素(rhANG)及其单克隆抗体(mAb)。方法采用凝胶扫描法分析rhANG的纯度;ELISA等方法检测抗rhANGmAb的特异性,鸡胚绒毛尿囊膜实验检测rhANG及其mAb的生物学活性,并与商品化标准品的免疫结合性进行对比研究。结果rhANG的纯度达96%,具有促进血管生长的生物学活性;抗ANGmAb具有很好的特异性和抑制血管生长的活性;而且二者较标准品均具有良好的免疫结合活性。结论该rhANG及其mAb可用于进一步的实验和临床应用研究。

Objective: Our purpose was to study the concentration of soluble tumor necrosis factor receptor Ⅰ(sTNFR Ⅰ) in the serum of patients with acute tuberculosis. Methods: The levels of soluble TNFR Ⅰ in serum were measured in 11 patients with acute tuberculosis. Concentration of sTNFR Ⅰ were measured by using enzyme linked immunological binding assay. Results: In patients with acute tuberculosis, especially severe tuberculosis, serum levels of sTNFR Ⅰ increased significantly (P<0.01), compared with...

Objective: Our purpose was to study the concentration of soluble tumor necrosis factor receptor Ⅰ(sTNFR Ⅰ) in the serum of patients with acute tuberculosis. Methods: The levels of soluble TNFR Ⅰ in serum were measured in 11 patients with acute tuberculosis. Concentration of sTNFR Ⅰ were measured by using enzyme linked immunological binding assay. Results: In patients with acute tuberculosis, especially severe tuberculosis, serum levels of sTNFR Ⅰ increased significantly (P<0.01), compared with those of control. After antituberculosis therapy, serum levels of sTNFR Ⅰin patients with acute tuberculosis decreased by various degrees. Conclusion: Measurements of sTNFR Ⅰ in serum of patients with acute tuberculosis could be an important index in the judgement of the outcome of the disease.

目的 :探讨活动性结核患者血清可溶性 TNF受体 - (s TNFR- )水平的变化。方法 :用双抗体夹心EL ISA法检测血清 s TNFR- 水平。结果 :活动性结核 ,特别是播散性结核患者血清 s TNFR- 水平较正常对照组明显增高 (P <0 .0 1) ;随诊观察抗结核治疗后 ,患者血清 s TNFR- 水平有不同程度下降。结论 :监测血清 s TNFR- 水平可作为判断活动性结核患者病情转归的重要指标

 
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