The authors had proved that one fast-reacting sulfhydryl group also participated in the binding of GST-π with GSH , so that at least 4 different groups were the binding groups of each subunit. The specific modification of amino group by TNBS was also verified and the ionic linkages between GST-π and GSH were proposed.
A review desci bes the preparation of polymers with binding groups in the cavities of original template molecules throngh an imprinting procedure of the template for separation of organic compounds and optical resolution of racemates Selectivity in separation is also discussed.
Results Compared with sham operated rats, the mean blood pressure, cardiac weight, levers of smooth muscle actin and MAPK expression in the abdominal aorta binding groups were all significantly increased ( P <0.01).
The body weight was very significantly reduced in the three acupuncture groups as compared with the medication and binding groups (P<0.01). In the acupuncture groups,the period of quick weight reduction occurred in 1-7 sessions of treatment,with an average decrease of 6.87 g each time,and the period of slow weight reduction occurred in 8-32 sessions of treatment,with an average decrease of 0.81 g each time.
Detection of cation-binding groups of opioid receptors using correlation analysis
Three x-ray structures of class A β-lactamases (native, mutant, and with substrate) show the individual binding groups at the active site change their accessible volumes depending on substrate binding and mutant form.
The biosorption of Sr2+ was optimal at neutral and high pHs, but lower at acidic pH, due to a competition between Sr2+ and H+ ions on the cation binding groups of Azolla.
Pre-loading of the metal-binding groups with K+ increased the removal of heavy metals from solutionby the Azolla biofilter.
Ca2+ and Mg2+, bound with a higher affinity to theAzolla cation-binding groups, were exchanged during the secondary phaseof Sr2+ binding.
The particularity of crosslinking of ethylene - propylene (EPDM)elastomer has been studied using torque - time curve (at 150℃),equilibrium modulus and swelling, physico - property tests and infraredspectra (MIRIR). The torque of EPDM increases linearly with continually heatingat 150℃ after obtained equilibrium density of chemical crosslinkingwith sulfur, accelereators or DCP. Therefore, the optimum crosslinkingtime can not be obtained with torque - time curve (Viscosmeter), butit can be obtained with equilibrium...
The particularity of crosslinking of ethylene - propylene (EPDM)elastomer has been studied using torque - time curve (at 150℃),equilibrium modulus and swelling, physico - property tests and infraredspectra (MIRIR). The torque of EPDM increases linearly with continually heatingat 150℃ after obtained equilibrium density of chemical crosslinkingwith sulfur, accelereators or DCP. Therefore, the optimum crosslinkingtime can not be obtained with torque - time curve (Viscosmeter), butit can be obtained with equilibrium modulus or equilibrium swelling. This particularity of crosslinking of EPDM is produced by thecontinually chemical reactions of residual free sulfur, accelerators orDCP in EPDM, after formation of chemical crosslinking, and thesereactions produced the bound groups (such as - C=S or [R-O]~-)of polymer chain. The impact resilience of EPDM is independente of crosslinking,while it is characteristic of macromolecular entangement and itsreaction with fillers.
A review desci bes the preparation of polymers with binding groups in the cavities of original template molecules throngh an imprinting procedure of the template for separation of organic compounds and optical resolution of racemates Selectivity in separation is also discussed.
By using l-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC) ,2,4,6-trinitrobenzylsulfonic acid and diacetyl for the chemical modification of carboxyl,amino and guanido group of human glutathione S-transferase (GST-π) respectively to study the kinetics for inactivation of enzyme revealed that the corresponding moles of inhibitor needed for fully inactivation of the enzyme were 1.0, 1.08, 0.98/enzyme subunit, suggusting that only one mole of carboxyl, amino and quanido group for each participated in the active...
By using l-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC) ,2,4,6-trinitrobenzylsulfonic acid and diacetyl for the chemical modification of carboxyl,amino and guanido group of human glutathione S-transferase (GST-π) respectively to study the kinetics for inactivation of enzyme revealed that the corresponding moles of inhibitor needed for fully inactivation of the enzyme were 1.0, 1.08, 0.98/enzyme subunit, suggusting that only one mole of carboxyl, amino and quanido group for each participated in the active center of GST-π subunit. The substrate and its analogs, glutatione, S-hexylglutathione and S-octylglutathione, protected GST-π from modification by the above inhibitors and the rate constant of pseudo-first order reaction k1 was remarkably decreased in the presence of these protectors, indicting that carboxyl, amino and guanido groups were involved in the GSH binding site of GST-π. The authors had proved that one fast-reacting sulfhydryl group also participated in the binding of GST-π with GSH , so that at least 4 different groups were the binding groups of each subunit. The specific modification of amino group by TNBS was also verified and the ionic linkages between GST-π and GSH were proposed.
binding groups
结合基团
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