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suspension cultivation
相关语句
  悬浮培养
     Establishment and Suspension Cultivation of Silkworm Bombyx mori Cell Line BmN ZJ S
     家蚕悬浮培养细胞系的建立及悬浮培养
短句来源
  “suspension cultivation”译为未确定词的双语例句
     High Density Suspension Cultivation of Taxus chinensis Cells
     中国红豆杉悬浮细胞高密度培养
短句来源
     PANAX QUINQUEFOLIUM L. CELL SUSPENSION CULTIVATION IN AIR LIFT REACTOR
     气升式反应器中的西洋参细胞培养
短句来源
     EFFECTS OF SHEAR STRESS ON SUSPENSION CULTIVATION OF PLANT CELLS
     剪切力对植物细胞悬浮培养的影响
短句来源
     In the experiments, the medium for grape callus induction was screened and that for grape cell suspension cultivation was developed. Based on the comparison of several kinds of protocols, Fatima Ragueh's protocol was modified for grape UNA isolation.
     试验中,筛选出了一种适宜的葡萄愈伤组织诱导培养基和建立了一种适宜的葡萄悬浮细胞培养培养基,并在比较多种葡萄RNA提取方法的基础上,优化了Fatima Ragueh的方法。
短句来源
     Biodegradability for PLA/PHBHHx blends was investigated in detail by soil suspension cultivation and scanning electron microscopy (SEM).
     通过土壤悬浊培养降解法和扫描电子显微镜(SEM)分析对共混材料的生物降解性能进行了研究。
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  相似匹配句对
     The Cultivation of Suspension Cell of Syringa Pubescens
     小叶丁香的悬浮细胞培养研究
短句来源
     EFFECTS OF SHEAR STRESS ON SUSPENSION CULTIVATION OF PLANT CELLS
     剪切力对植物细胞悬浮培养的影响
短句来源
     Cultivation and Purification
     教化与净化
短句来源
     Berrytomatoes and its Cultivation
     樱桃番茄及其栽培技术
短句来源
     Suspension Conveyer
     悬挂输送机
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This study was performed to observe the erythrocytic schizogony of P. vivax under several culture conditions in vitro. Five experimental groups included: 1) static cultivation in candle jar; 2) static cultivation in candle jar with candle relightened; 3) static cultivation under low oxygen tension (5-10%); 4) deep suspension cultivation in test tubes with cotton plunger; 5) deep suspension cultivation in closed test tubes with screw cap. Two different isolates of P. vivax collected from Jingshan...

This study was performed to observe the erythrocytic schizogony of P. vivax under several culture conditions in vitro. Five experimental groups included: 1) static cultivation in candle jar; 2) static cultivation in candle jar with candle relightened; 3) static cultivation under low oxygen tension (5-10%); 4) deep suspension cultivation in test tubes with cotton plunger; 5) deep suspension cultivation in closed test tubes with screw cap. Two different isolates of P. vivax collected from Jingshan County and Zaoyang County were used. Cultivation was initiated with two methods, i.e. direct inoculation from fresh patient blood with malaria parasites and retrieval cultivation from freezing malaria parasite blood. The suspension cultivation in test tube with cotton plunger could not support the schizogony of P. vivax, while other groups could at least complete two schizogony cycles. The best result was obtained with static cultivation under low oxygen tension, the growth of parasites appeared to be more normal. The results showed that cultivation of P. vivax under a low oxygen concentration of 5-10% is preferred and the selection of isolates of P. vivax might be important in in vitro cultivation.

在培养液成分基本不变的情况下,观察几种体外培养条件的间日疟原虫红内裂体增殖.实验组为:烛缸静止培养,重复点烛烛缸静止培养,低氧静止培养,两种试管深层悬浮培养。引种用了两个虫株,起始培养采用冷冻虫血复苏和现症疟疾病人新鲜虫血直接引种。除棉塞试管深层悬浮组不能支持裂体增殖外,其他组均至少不同程度地完成两个裂殖周期;在低氧下,原虫发育更正常。观察发现,间日疟原虫分离株在体外培养中是重要的。

The release of free-living vegetative cells were successfully achieved by enzymolysis of Porphyra haitanensis fronds.The fronds with good configuration, such as color and length,were selected from field net in Xiangshan,Zhejiang Province. The blades were dried in darkness at room temperature and stored at - 20 ℃ .Before experiment, the fronds were taken out and immerged into seawater for two or four days to make cell retrieve activity. After enzymolysis, single somatic cells were picked out into 96 well microplates...

The release of free-living vegetative cells were successfully achieved by enzymolysis of Porphyra haitanensis fronds.The fronds with good configuration, such as color and length,were selected from field net in Xiangshan,Zhejiang Province. The blades were dried in darkness at room temperature and stored at - 20 ℃ .Before experiment, the fronds were taken out and immerged into seawater for two or four days to make cell retrieve activity. After enzymolysis, single somatic cells were picked out into 96 well microplates under invert microscope, with the help of capillary tube. Then single cell was cultivated at 20 ℃ , 12L-12D and light density 1500 - 2 000 lx. The medium was refreshed after 3 to 5 d.The development of somatic cells was observed continuously and described as follows:in the procedure of cultivation,majority of cells died only except a small portion of the somatic cell which can develop into conchocelis thallus, blade and calluse-like cell group.Conchocelis thalli can be formed through two routes:in the first case, single somatic cell produced pustutes from some parts of the cell,then these pustutes elongated and produced forks, finally grew into conchocelis thalli. In this case,some cells only produced pustutes from the parts where pigments concentrated, and the others produced pustutes too,but cytoplasm color change couldn' t be observed under microscope. In the second case, somatic cell formed callus-like cell group primarily. After cultivated for some time,the cal-lus-like cell guoup disintegrated and released spore-like cells. These spores developed into conchocelis thalli, while a few spore-like cells germinated into small blades. But some cell.groups didn't break,just formed conchocelis thalli from the live cells in the cell-group. In order to distinguish the development of somatic cell from sexual cell (carpogonium and carpospore) , single marginal cells of female blade were cultivated as the method as somatic cell cultivation. The results showed that it was effective to differentiate somatic cell and carpospore by the size and germinating fashion. It was important for the establishment of pure line diploid conchocelis.After three months or more, these conchocelis thalli produced from single cell,as pure lines, were cultivated largely through suspension cultivation method to multiplied, and further cultured the same as the tra-ditional mariculture. Comparing with un-selected line, the pure lines showed some superior traits, including the output of blades and other economic characters.On the point of pure line establishment,from haploid blade cell to diploid conchocelis thallus, the origin genetic material was reduplicated directly.So this cultivation method gives a guarantee to descendant blades to possess high similarity in genetic background. This technique has two conceivable advantages which are preventing the loss of desired economic characteristics and shortening the cultivating time compared with traditional pure line establishemt method.

用海螺酶酶解挑选出的具有优良性状的坛紫菜(Porphyra haitanensis)叶状体制备游离的营养细胞,在显微镜下选取单个细胞放入96孔板中进行隔离培养。一部分细胞通过2种发育途径形成了丝状体:一种途径是单细胞生长一段时间产生突起后发育形成丝状体;另一种途径是单细胞先形成愈伤组织,愈伤组织解体放散出类似"孢子"的细胞,"孢子"再发育形成丝状体。将获得的丝状体扩大培养作为纯系并下海养殖。同普通品系相比,选育的品系在产量和质量上表现出一定的优势。在纯系培育方面,由单倍的叶状体细胞发育形成的二倍丝状体,遗传物质一步纯合,后代叶状体个体遗传性状高度一致,可以保证优良经济性状不丢失。这种紫菜纯系培育方法大幅度缩短了纯系培育周期。

Poly(lactic acid)/poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PLA/PHBHHx) blends were prepared by melt mixing method. Mechanical properties of the blends were investigated by tensile mechanical test. Biodegradability for PLA/PHBHHx blends was investigated in detail by soil suspension cultivation and scanning electron microscopy (SEM). Experimental results indicated that increasing PHBHHx content led to decrease in tensile strength and modulus, and yet significantly increase the rate of biodegradation...

Poly(lactic acid)/poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PLA/PHBHHx) blends were prepared by melt mixing method. Mechanical properties of the blends were investigated by tensile mechanical test. Biodegradability for PLA/PHBHHx blends was investigated in detail by soil suspension cultivation and scanning electron microscopy (SEM). Experimental results indicated that increasing PHBHHx content led to decrease in tensile strength and modulus, and yet significantly increase the rate of biodegradation of the blends. But the rate of weight-loss of PLA/PHBHHx blends (20/80) was faster than that of pure PHBHHx. PLA/PHBHHx blends, especially the blends containing 20/80 (wt/wt), possess a good combination of mechanical properties and biodegradation rate.

通过熔融共混法制备了聚乳酸/微生物产β-羟基丁酸酯与β-羟基己酸共聚物的共混物(PLA/PHBHHx)。采用拉伸力学试验研究了共混物的力学性能。通过土壤悬浊培养降解法和扫描电子显微镜(SEM)分析对共混材料的生物降解性能进行了研究。实验结果表明,随着PHBHHx含量的增加,共混物的拉伸强度和杨氏模量降低,而生物降解速率却显著提高。但是,在175h之前,重量组成比为20/80的共混物降解速率比纯PHBHHx还要快。综合分析表明,共混材料PLA/PHBHHx的重量比为20/80时,具有优良的力学性能和生物降解性。

 
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