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antifungal metabolites
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  抗菌代谢物
     The pooI deleted mutant did not evidently affect production of antifungal metabolites, such as 2,4-DAPG, HCN and siderophores, but significantly affected biofilm formation, colonization on wheat rhizosphere and adaption of environment.
     通过对pcoI基因的缺失突变和互补分析发现,pcoI至少合成3种QS信号,而且QS系统与抗菌代谢物2,4-DAPG,HCN和嗜铁素的产生无显著关系,而与biofilm的形成,小麦根部的定殖,环境适应性密切相系。 温室生测表明,pcoI突变体对小麦全蚀病和番茄青枯病的防效显著低于野生型。
短句来源
     fluorescens CHA0. Further research revealed that thegacS_knock_out mutant was unable to produce antifungal metabolites, such as 2,4_DAPG, HCN and proteinase.
     与野生菌株2P2 4相比,gacS基因的缺失突变体完全丧失产生抗菌代谢物2 ,4_二乙酰基间苯三酚、氢氰酸、蛋白酶的能力。
短句来源
  活性代谢产物
     During the course of the investigation of the antifungal metabolites against Peronophythora litchii from the fermentation broth of Streptomyces sp SC120, an antibiotics that exhibited the cytotoxicity to CNE 2 was isolated By spectral (UV, IR, HRMS, 1H NMR, 13 C NMR, 1H 1H COSY, HMQC and HMBC) analyses, its structure was identified as pimprinine The cytotoxicity of pimprinine to CNE 2 was reported for the first time
     在研究链霉菌Streptomycessp SC1 2 0抗荔枝霜疫霉菌活性代谢产物的过程中 ,分离得到一对人鼻咽癌细胞具有细胞毒作用的抗生素。 通过UV、IR、HR MS、 1H NMR、13C NMR、DEPT、1H 1HCOSY、HMQC、HMBC等波谱分析 ,鉴定其结构与Pimprinine相同。
短句来源
     Objective To investigate the antibacterial and antifungal metabolites from the fermentation broth of Streptomyces sp.M095 and classify the strain M095.Methods The structure was evaluated by spectral(ESI-MS,EI-MS,~(1)H-NMR,~(13)C-NMR and APTNMR) analysis.
     目的研究胶州湾海洋链霉菌M095抑菌活性代谢产物及菌株M095的分类。 方法采用ESI-MS,EI-MS,1H-NMR,13C-NMR和APTNMR等波谱分析进行结构测定;
短句来源
  “antifungal metabolites”译为未确定词的双语例句
     Antifungal metabolites of a Streptomyces sp.SC120 antagonistic to Peronophythora litchii
     荔枝霜疫霉拮抗菌株链霉菌sp.SC120抗真菌活性代谢物的研究
短句来源
     Antifungal Metabolites of Endophytic Fungus A10 in Celastrus angulatu
     苦皮藤内生真菌A10代谢产物的杀菌活性
短句来源
     Antifungal Metabolites from a Marine-derived Bacillus subtilis Bs-1: Production and Partial Characterization
     海洋枯草芽孢杆菌Bs-1产生多种抗真菌活性物质
短句来源
     The further research revealed that the knock-out mutant of gacS gene was unable to produce antifungal metabolites such as 2,4-DAPG, HCN and proteinase. It also lost the ability to inhibit the growth of phytopathogens in Petri dish and to control the development of wheat take-all in greenhouse.
     对gacS基因进行突变和基因互补,通过功能互补证明GacS严格控制2,4-DAPG等次生抗菌物质和QS信号的产生,并与生防能力呈显著正相关。
短句来源
     The study of antibiotic-producing condition o f Bs-1 demonstrated that the growth of Bs-1 in PDB is very rapid and the antibio tic production was detected at the time point of 6 h. Surprisingly, Bs-1 did n ot produce any antifungal metabolites in artificial sea water medium, LB, YPD an d BPY.
     对Bs_1产生抗生素条件的研究表明 ,Bs_1在多种培养基中生长迅速 ,但只在PDB (马铃薯葡萄糖液体 )中产生抗真菌活性物质 ;
短句来源
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  相似匹配句对
     Marine Fungi and Their Antifungal Bioactive Metabolites
     海洋真菌及其抗真菌生物活性物质
     Antifungal Metabolites of Endophytic Fungus A10 in Celastrus angulatu
     苦皮藤内生真菌A10代谢产物的杀菌活性
短句来源
     The Metabolites of Quinocetone in Pigs
     喹烯酮在猪体内的代谢物研究
短句来源
     4 metabolites were identified.
     氧化和水解是瑞芬太尼的主要代谢反应,瑞芬太尼在人体中形成4种代谢物。
短句来源
     Advances in antifungal agents
     抗真菌药物的研究进展
短句来源
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  antifungal metabolites
Characterization of Antifungal Metabolites Produced by Penicillium Species Isolated from Seeds of Picea glehnii
      
The results of this study indicated the role of chitinase and β-1,3-glucanase in the inhibition of R.?solani, however, antifungal metabolites of a non-enzymatic nature were responsible for inhibition of P.?capsici.
      
Determining the Suitability of Lactobacilli Antifungal Metabolites for Inhibiting Mould Growth
      
Production and Chemical Characterization of Antifungal Metabolites From Micromonosporasp.
      
harzianum, while the?antifungal metabolites of T.?harzianum completely prevented the linear growth of B.
      
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During the course of the investigation of the antifungal metabolites against Peronophythora litchii from the fermentation broth of Streptomyces sp SC120, an antibiotics that exhibited the cytotoxicity to CNE 2 was isolated By spectral (UV, IR, HRMS, 1H NMR, 13 C NMR, 1H 1H COSY, HMQC and HMBC) analyses, its structure was identified as pimprinine The cytotoxicity of pimprinine to CNE 2 was reported for the first time

在研究链霉菌Streptomycessp SC1 2 0抗荔枝霜疫霉菌活性代谢产物的过程中 ,分离得到一对人鼻咽癌细胞具有细胞毒作用的抗生素。通过UV、IR、HR MS、 1H NMR、13C NMR、DEPT、1H 1HCOSY、HMQC、HMBC等波谱分析 ,鉴定其结构与Pimprinine相同。首次报道Pimprinine对人鼻咽癌细胞具有细胞毒作用

The antifungal substances secreted from a marine-derived Bacillus subtilis Bs-1 was studied in the present short report. Bs-1 was isolated from sea water samples of Clear Water Bay of Hong Kong and the PDB culture broth extract of Bs-1 can strongly inhibit the growth of y east-like fungi and G+ bacteria. The study of antibiotic-producing condition o f Bs-1 demonstrated that the growth of Bs-1 in PDB is very rapid and the antibio tic production was detected at the time point of 6 h. Surprisingly,...

The antifungal substances secreted from a marine-derived Bacillus subtilis Bs-1 was studied in the present short report. Bs-1 was isolated from sea water samples of Clear Water Bay of Hong Kong and the PDB culture broth extract of Bs-1 can strongly inhibit the growth of y east-like fungi and G+ bacteria. The study of antibiotic-producing condition o f Bs-1 demonstrated that the growth of Bs-1 in PDB is very rapid and the antibio tic production was detected at the time point of 6 h. Surprisingly, Bs-1 did n ot produce any antifungal metabolites in artificial sea water medium, LB, YPD an d BPY. The activity of the crude extract was very stable after had been heated t o 121 ℃ for 15 min, and pH values from 2 to 10 for 24 h. A procedure was perfor med to purify the antifungal metabolites using orderly adsorption, DEAE-Sephadex A-25 ion-exchange chromatography and HPLC. The result showed that Bs-1 can at l east produce three kinds of antifungal component in PDB. The structure of purifi ed component Ⅱ was preliminary characterized. The results demonstrated that thi s component contained 2 conjugated bonds but did not contain any amino acid re sidue, and was resistance to detergent and digestion of proteinase K, which is d ifferent from the known metabolites from other strains of B. subtilis, sugge sting that it probably is a novel antibiotic.

从香港清水湾海域中分离到一株能产生抗酵母样真菌和革兰氏阳性菌抗生素的枯草芽孢杆菌菌株Bs_1。对Bs_1产生抗生素条件的研究表明 ,Bs_1在多种培养基中生长迅速 ,但只在PDB (马铃薯葡萄糖液体 )中产生抗真菌活性物质 ;活性物质粗提液具有良好的耐热性和酸碱度 ,12 1℃加热 15min及在pH 2和pH 10处理 2 4h仍有相当高的活性 ;对Bs_1产抗生素的营养条件和影响的理化因子进行了分析 ;最后用吸附、萃取、离子交换层析和RP_HPLC等技术对此菌的抗菌代谢产物进行纯化 ,结果发现Bs_1在PDB培养基中发酵时至少产生 3种亲水性的抗真菌活性化合物 ,三者在C18反相层析柱上的保留时间差别不大 ,对HPLC纯化后的其中一种化合物结构的初步分析表明 ,该化合物含有共轭双键但无任何氨基酸残基 ,与目前已知的枯草芽孢杆菌产生的抗生素都不相同 ,很可能是一种新的非肽类抗真菌化合物。

Pseudomonasfluorescens 2P24, a biocontrol agent for soil_borne diseases has been isolated from the wheat take_all decline soil, and was characterized with efficient production of antifungal compounds. In this study, thegacSgene was cloned by PCR from theP. fluorescens 2P24 genomic library. Nucleotide sequencing indicated that thegacS gene contains 2754 bp, and is predicted to encode a peptide of 917 amino acids with molecular mass of 101 kD. The deduced amino acid sequence shares 91% identity to that of the...

Pseudomonasfluorescens 2P24, a biocontrol agent for soil_borne diseases has been isolated from the wheat take_all decline soil, and was characterized with efficient production of antifungal compounds. In this study, thegacSgene was cloned by PCR from theP. fluorescens 2P24 genomic library. Nucleotide sequencing indicated that thegacS gene contains 2754 bp, and is predicted to encode a peptide of 917 amino acids with molecular mass of 101 kD. The deduced amino acid sequence shares 91% identity to that of the sensor kinase GacS ofP. chlororaphis and 89% identity to GacS ofP. fluorescens CHA0. Further research revealed that thegacS_knock_out mutant was unable to produce antifungal metabolites, such as 2,4_DAPG, HCN and proteinase. Moreover, it lost the ability to inhibit the growth ofGaeumannomyces graminis in Petri dish and to control the development of wheat take_all disease in greenhouse. The complemented strain containing a plasmid_bearinggacS gene could restore all of the lost phenotypes. The results indicated that GacS in an important regulatory factor and directly controls the synthesis of key biocontrol factors and the biocontrol efficacy ofP. fluorescens 2P24.

荧光假单胞杆菌2P2 4菌株分离自小麦全蚀病自然衰退土壤,可产生多种次生抗菌物质,对一些作物土传病害具有较好的防治能力。通过PCR介导的方法从荧光假单胞杆菌2P2 4的基因组文库中克隆到调控基因gacS。序列分析发现,该基因长度为2 75 4bp ,编码917个氨基酸的肽链。此肽链与Pseudomonaschlororaphis双因子组分之一的感受激酶GacS相似性达91% ,与P .fluorescensCHA0的GacS相似性为89%。与野生菌株2P2 4相比,gacS基因的缺失突变体完全丧失产生抗菌代谢物2 ,4_二乙酰基间苯三酚、氢氰酸、蛋白酶的能力。拮抗试验中,gacS缺失突变体丧失对小麦全蚀病菌的拮抗作用,温室生物测定显示gacS的缺失突变体对小麦全蚀病的生防能力大幅下降。但是gacS基因的互补突变体能够恢复产生抗菌次生代谢物的能力,且重新获得拮抗能力和生防能力。由此证明GacS是生防菌株2P2 4中一个控制生防因子并影响生防效果的重要调控元件。

 
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