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similarity alignment
相关语句
  相似性比较
     Similarity alignment showed that the fragment is 89% identical at amino acid level to the corresponding region of the known An. gambiae EST sequence, as well as 63% identical to that of both the fruitfly and human sequence.
     相似性比较显示该编码序列在氨基酸水平与已知的冈比亚按蚊EST序列对应部位的同源性为 89% ,与果蝇和人类的同源性均为 63%。
短句来源
     Sequence similarity alignment of the 255 universal ESTs containing SSRs against database in GenBank indicated that 63 ESTs (30%) were associated with protein destination, and the rest were involved in metabolism, transcription, cellular development, defense system and other pathways related.
     序列相似性比较发现 ,2 5 5个通用EST SSRs标记所对应的基因 80 %功能已知 ,其中 30 %与蛋白质定位相关 ,其余 70 %参与细胞代谢、转录调控、细胞发育、防御体系及其他所涉及的生理生化过程。
短句来源
  相似性比较发现
     Sequence similarity alignment of the 255 universal ESTs containing SSRs against database in GenBank indicated that 63 ESTs (30%) were associated with protein destination, and the rest were involved in metabolism, transcription, cellular development, defense system and other pathways related.
     序列相似性比较发现 ,2 5 5个通用EST SSRs标记所对应的基因 80 %功能已知 ,其中 30 %与蛋白质定位相关 ,其余 70 %参与细胞代谢、转录调控、细胞发育、防御体系及其他所涉及的生理生化过程。
短句来源
  “similarity alignment”译为未确定词的双语例句
     Reassortment was not found between CMV and PSV in CMV-CA, CS and PSV-Mi by similarity alignment, though PSV and CMV were found in the same infecting area.
     上述分析均证实我国花生CMV-CA和CS两个毒力不同的株系均属于CMV-IB亚组。
短句来源
     Methods: Through similarity alignment of the conservative transmembrane domain of human MCP1 receptor, mouse MIP1α receptor and rat IL8 receptor, the degenerate oligonucleotide homology primers were designed and a cDNA fragment was amplified by RTPCR. After a new gene sequence of chemokine receptor supergenefamily was identified by GenBank, the new gene full sequence was cloned by a Marathon PCR of the specific primers.
     方法:将β-趋化因子受体小鼠MIP-1αR,人MCP-1R和大鼠IL-8R跨膜区中高度保守的氨基酸序列中的核苷酸进行相似序列对比后,设计出同源引物,再用RT-PCR扩增出DNA片段,经基因库检索为该超基因家族中的新基因序列后设计特异引物,用MarathonPCR扩增出该新基因。
短句来源
     Similarity alignment showed that the products of the cloned cDNA were very similar to those of insect esterases.
     经blastx检索genebank,发现此cDNA产物与其它昆虫抗性酯酶基因有高度的相似性。
短句来源
  相似匹配句对
     STRUCTURAL ALIGNMENT IN SIMILARITY
     类似性结构组合的实验研究
短句来源
     Alignment-based biological sequences similarity analysis
     基于对齐的生物序列相似性分析
短句来源
     On the Similarity in Drawing
     论绘画中的“相似性”
短句来源
     RECURSIVE SIMILARITY
     递归相似
短句来源
     Alignment for CSR
     CSR的准直安装
短句来源
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  similarity alignment
Based on the similarity alignment with its closest homologs, the students also confirmed or reassigned the start codon for each gene.
      


Objective:Using homology primers to clone βchemokine receptor supergenefamily from lower level RNA expression. Methods: Through similarity alignment of the conservative transmembrane domain of human MCP1 receptor, mouse MIP1α receptor and rat IL8 receptor, the degenerate oligonucleotide homology primers were designed and a cDNA fragment was amplified by RTPCR. After a new gene sequence of chemokine receptor supergenefamily was identified by GenBank, the new gene full sequence was cloned by a Marathon...

Objective:Using homology primers to clone βchemokine receptor supergenefamily from lower level RNA expression. Methods: Through similarity alignment of the conservative transmembrane domain of human MCP1 receptor, mouse MIP1α receptor and rat IL8 receptor, the degenerate oligonucleotide homology primers were designed and a cDNA fragment was amplified by RTPCR. After a new gene sequence of chemokine receptor supergenefamily was identified by GenBank, the new gene full sequence was cloned by a Marathon PCR of the specific primers. Results: Mouse CCR5 fulllength cDNA with 2 888 bp was cloned, including open reading frame 1 065 oligonucleotides and coding 355 amino acids. This gene was a novel member of the βchemokine receptor supergenefamily confirmed by sequence analysis, ligand binding assay and GenBank retrieval. Conclusion: Using homology primers, the novel member of the supergenefamily can be captured. This method is a new cloning strategy and has extensive significance in the molecular cloning of a new gene.

目的:用同源引物克隆RNA低丰度表达的小鼠β-趋化因子受体。方法:将β-趋化因子受体小鼠MIP-1αR,人MCP-1R和大鼠IL-8R跨膜区中高度保守的氨基酸序列中的核苷酸进行相似序列对比后,设计出同源引物,再用RT-PCR扩增出DNA片段,经基因库检索为该超基因家族中的新基因序列后设计特异引物,用MarathonPCR扩增出该新基因。结果:成功克隆出小鼠β-趋化因子受体5(CCR5)cDNA全基因序列,序列全长2888bp,开放阅读框1065个核苷酸,编码355个氨基酸。该序列经序列分析、配体结合试验及国际基因库检索证实为该超基因家族中的一个新基因成员。结论:该克隆策略的引物设计思路较为新颖,能有效捕获超基因家族中的新基因。该克隆方法敏感、高效,对基因家族新基因的克隆具有广泛的意义

It is a normal strategy to clone protein family cDNA using the degeneracy of the code to design PCR primers from protein multiple\|sequence alignments. With the tools of blastp, blockmaker, CodeHop, SwissProt,and SpTrEMBL, degenerate primers were designed for cloning of the insect esterase cDNA. Using one pair of primers, a partial cDNA was obtained from the diamondback moth by RT\|PCR. Similarity alignment showed that the products of the cloned cDNA were very similar to those of insect esterases....

It is a normal strategy to clone protein family cDNA using the degeneracy of the code to design PCR primers from protein multiple\|sequence alignments. With the tools of blastp, blockmaker, CodeHop, SwissProt,and SpTrEMBL, degenerate primers were designed for cloning of the insect esterase cDNA. Using one pair of primers, a partial cDNA was obtained from the diamondback moth by RT\|PCR. Similarity alignment showed that the products of the cloned cDNA were very similar to those of insect esterases. The results indicated that these degenerate primers designed by this software could be used to obtaining specific RT\|PCR product.

利用遗传密码简并性 ,针对特定的氨基酸序列设计简并引物 ,是克隆蛋白质家族cDNA的常规方法。文章介绍了利用blastp ,blockmaker,CodeHop ,SwissProt,SpTrEMBL等网络工具及数据库设计昆虫抗性酯酶的简并引物。用这对引物从抗有机磷杀虫剂的小菜蛾Plutellaxylostella中克隆了 1段cDNA。经blastx检索genebank,发现此cDNA产物与其它昆虫抗性酯酶基因有高度的相似性。研究表明 ,程序化设计的简并引物可信性强 ,阳性率高 ,能迅速得到满意结果

The isolation and study of genes that are differentially expressed in malaria infected mosquitoes is important for the elucidation of basic molecular mechanisms underlying vector parasite interactions. When screening against a previously established cDNAs pool representing specifically expressed genes in the mosquito Anopheles stephensi infected by Plasmodium yoelii, it was found that one of these encodes a protein with extensive sequence similarity to the Drosophila melanogaster ubiquitin...

The isolation and study of genes that are differentially expressed in malaria infected mosquitoes is important for the elucidation of basic molecular mechanisms underlying vector parasite interactions. When screening against a previously established cDNAs pool representing specifically expressed genes in the mosquito Anopheles stephensi infected by Plasmodium yoelii, it was found that one of these encodes a protein with extensive sequence similarity to the Drosophila melanogaster ubiquitin C terminal hydrolase(UCTH). Similarity alignment showed that the fragment is 89% identical at amino acid level to the corresponding region of the known An. gambiae EST sequence, as well as 63% identical to that of both the fruitfly and human sequence. Virtual Northern blot expression dynamics of the gene indicated that it was up regulated significantly in the mosquito at least 1 7 days post infection, consistent with the critical transition stages of midgut invasion and relocation of sporozoites from the oocysts to the salivary glands during parasite development. Rather little is known about the role of the ubiquitin pathway in the activation of the mosquito innate immune system. The results indicate that the gene is related to malaria infection in mosquito. The cloning and expression profile analysis of As UCTH enables us to make predictions as to the roles it may play during malaria infection.

分离和研究疟疾感染蚊的差异表达基因 ,对阐明媒介与疟原虫之间相互作用及其分子机制尤为重要。利用已建立的斯氏按蚊感染约氏疟原虫的差减cDNA库的进行表达筛选 ,发现表达增高基因中有一个编码与黑腹果蝇泛素羧端水解酶高度同源蛋白的序列。相似性比较显示该编码序列在氨基酸水平与已知的冈比亚按蚊EST序列对应部位的同源性为 89% ,与果蝇和人类的同源性均为 63%。模拟Northern印迹的表达动态分析提示 ,感染后至少 1~ 7天内该基因在蚊体内的表达显著增高 ,与疟原虫发育动合子穿越蚊中肠壁和子孢子从卵囊向蚊眼涎腺移行等关键阶段相一致。目前对有关蚊天然免疫系统激活的泛素途径所知甚少 ,现有结果提示该基因与疟原虫感染相关 ,它的克隆和表达分析有可能推测其在疟原虫感染中所起的作用

 
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