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binding motif
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  结合基序
     FBL-3 is a transplantable Friend virus-induced leukemia of B6 (H-2b) origin,which can induce the CTL against FBL-3 by immunizing B6 mice with atenuated FBL-3.Seven peptides were predicted as candidates of FBL-3 antigenic peptides in the light of H-2Db specific peptide binding motif and gag gene sequence of Friend virus.
     FBL-3是B6小鼠(H-2~b)源由Friend病毒诱导的小鼠白血病细胞,灭活FBL-3免疫B6小鼠可诱导FBL-3特异CTL产生,从而建立FBL-3特异的免疫应答,保护小鼠免受FBL-3的致瘤性。 因此,实验中根据H-2D~b多肽结合基序和Friend病毒gag基因序列,预测出7个可能编码FBL-3抗原多肽的片段。
短句来源
     The analysis of the special protein motif of ORF shown that the Zinc finger motif can be found in 2 ORFs, Leucine Zipper motif can be found in 3 ORFs, the NTP binding motif can be found in 2 ORFs, and the nuclear localization signal motif can't be found in 14 ORFs. ORF S13 has alignment protein by BLAST in NCBI database.
     ORF转译产物的特性基序分析显示 :有 2个ORFs可检出锌指基序 ,3个ORFs可检出亮氨酸拉链基序 ,2个ORFs可检出NTP结合基序 ,未检出核定位信号基序。
短句来源
     Methods: Two HER2/neu peptides compatible with H 2K d binding motif as tumor rejection antigens were synthesized. The ability of inducing peptide specific CTLs and suppressing the tumor growth in BALB/c mice immunized by HER2/neu peptide was analyzed.
     方法 :利用合成的2个具有小鼠H 2Kd 分子结合基序的HER2 /neu肽作为肿瘤排斥抗原 ,在体外刺激小鼠脾淋巴细胞以及皮下免疫小鼠 ,观察淋巴细胞的增殖能力、CTL的杀伤活性以及HER2 /neu肽的抑瘤作用。
短句来源
     A "Finding epitope"programme with the function of searching short peptides was written and a computer scoring programme deduced from the HLA-A2 specific peptide binding motif was developed.
     本文设计了一个具有查找HLA分子结合多肽功能的“Findingepitope”程序,并针对HLA-A2分子特异的多肽结合基序(peptidebindingmotif)建立了一个计算机评分系统。
短句来源
     Methods The HLA typing was determined serologically in two chronic HCVpatients. Batal on this, the HLA restricted CTL epitopes were predicted by HLA binding motif.
     方法血清学法检测了2例慢性HCV感染者的HLA分型,并以HLA结合基序为依据,预测了HLA限制的CTL表位。
短句来源
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  “binding motif”译为未确定词的双语例句
     The LGBP contains a potential N-glycosylation site (294-296), a potential polysaccharide binding motif (313-330), a glucan binding motif (341-350).
     LGBP 有一个 N-糖基化位点(294-296)、一个脂多糖结合位点(313-330)及一个葡聚糖结合位点(341-350)。
短句来源
     It encodes a BTB/POZ domain at the N-terminus and a PCNA binding motif (QTKV-EFP) at the C-terminus.
     该蛋白的N端具有一个BTB/POZ结合域和C端具有一个PCNA的保守结合域(QTKV-EFP)。
短句来源
     To study the optimal induction of HBV antigen specific T cells (HBV CTLs),the PBMC isolated from healthy donors blood were costimulated with the synthetic peptides derived from HBcAg (PepⅠ:amino acid 18 ̄27 and PepⅡ:aa 141 ̄151),which were equal to the published HLA A2 binding motif (CTL epitope).
     根据乙肝病毒核心抗原(HBcAg)的MHC-Ⅰ类分子结合关键序列合成抗原性多肽2段(PepⅠaa:18~27及PepⅡaa:141~151),分别与转铁蛋白(Tf)、抗CD3的单克隆抗体(CD3McAb)和IL-2联合体外诱导乙肝病毒抗原特异性细胞毒T细胞(HBV-CTLs),动态观察其增殖状态和细胞毒活性。
短句来源
     However, both the DREB1A and DREB2A proteins contain a typical EREBP/AP2 binding motif. They all belong to EREBP/AP2-type protein family.
     DREB1蛋白和DREB2蛋白之间,在整体的氨基酸序列上没有同源性,但这两个转录因子都含有一个十分保守的EREBP/AP2结构域,属于EREBP/AP2类蛋白家族。
短句来源
     Sex determining genes Mab-3 of C. elegans and Doublesex of Drosophila, human Doublesex related gene DMRT1 and medaka sex-determining gene DMRT1Y/DMY contain a common DNA binding motif called DM(Doublesex and Mab-3)domain, both of which regulate similar aspects of sexual development.
     已经发现果蝇Doublesex、线虫Mab-3、青DMRT1Y/DMY和人类DMRT1等性别决定与分化基因均含有一个具有DNA结合能力的保守基序--DM结构域,对性别决定和性别分化具有调控功能.
短句来源
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  相似匹配句对
     This gene has a conserved ATP binding motif.
     该基因具有典型的ATP结合模体GAGDQG。
短句来源
     CTL epitopes were predicted using MHC binding motif.
     根据基序( motif) 及 H L A 分型资料预测该区段中的 C T L 抗原表位。
短句来源
     Analysis on Motif
     作为结构形式的母题分析——语言批评方法论之二
短句来源
     On the motif of district
     小区主题的探讨
短句来源
     protein binding;
     蛋白结合;
短句来源
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  binding motif
The 14-3-3-binding motif Lys-Lys-Glu-Ser-His-Pro (371-376) was detected in the HvNHX2 amino acid sequence, which is suggestive of possible involvement of the 14-3-3 proteins in the regulation of HvNHX2 function.
      
Next, H+-pump activity was reduced through microinjection of a phosphorylated peptide matching the canonical 14-3-3 binding motif RSTpSTP in the acid cell region.
      
Next, H+-pump activity was reduced through microinjection of a phosphorylated peptide matching the canonical 14-3-3 binding motif RSTpSTP in the acid cell region.
      
The 14-3-3-binding motif Lys-Lys-Glu-Ser-His-Pro (371-376) was detected in the HvNHX2 amino acid sequence, which is suggestive of possible involvement of the 14-3-3 proteins in the regulation of HvNHX2 function.
      
Comparison of bacterial and phage display peptide libraries in search of target-binding motif
      
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n contrast to many sequence-specific DNA binding proteins,for which DNA binding motifs such as thehelix-turn-helix,zinc-finger and leucinezipper have been well understood,a quite new mode of DNA bindingis identified as the S(T)PXX motif After proposing the role of a SPXXXGXP motif of peptides in DNA bind-ing process,we show here that the motif specified above prefers to recognize and bind to DNA with muchlower sequence specificity,using DNA cellulose AC. The novel results...

n contrast to many sequence-specific DNA binding proteins,for which DNA binding motifs such as thehelix-turn-helix,zinc-finger and leucinezipper have been well understood,a quite new mode of DNA bindingis identified as the S(T)PXX motif After proposing the role of a SPXXXGXP motif of peptides in DNA bind-ing process,we show here that the motif specified above prefers to recognize and bind to DNA with muchlower sequence specificity,using DNA cellulose AC. The novel results show that HPAc method is valuablefor producing accurate,highly reproducible features in elucidating the structural and molecular mechanisms ofnon-specific protein-DNA interaction.

高效亲和色谱研究含有特定结构域肽与DNA的结合张若蘅,杨栩,李崇熙,徐筱杰(北京大学化学系北京100871)1前言在脱氧核糖核酸(DNA)结合蛋白质中,除了螺旋-转折。螺旋、锌指和亮氨酸拉链三种主要结构域之外 ̄[1],近年来,一类含有β-转折结构的S...

Genes are regulated by the DNA-binding proteins which bind to the specificsites of the DNA,Based on the crystallographic and molecular biology study,the DNA-proteininteractions are elucidated.DNA-binding motifs such as“helix-turn-helix”,“zinc-finger” and“leucine-zipper”are discussed。

DNA结合蛋白通过与特定位点的DNA结合可进行基因功能的调控。根据蛋白质结晶学和分子生物学研究了蛋白质-DNA之间的相互作用。对几种DNA结合域,如“螺旋-转折-螺旋”、“亮氨酸拉链”、“锌指”进行了讨论

CD8+ T cells play an important role in the immunologic control of intracellular pathogens,particularly viruses,Leishmania are obligate intracellular parasites of macrophages in the mammalian host,and previous studies using deletion of CD8+ cells by administration of mAb to infected animals have suggested a protective role for these cells.Two complementary approaches were used to define more carefully the role of CD8+ cells in leishmaniasis.In BALB/c mice susceptible to Leishmania major(L. major)infection, targeted...

CD8+ T cells play an important role in the immunologic control of intracellular pathogens,particularly viruses,Leishmania are obligate intracellular parasites of macrophages in the mammalian host,and previous studies using deletion of CD8+ cells by administration of mAb to infected animals have suggested a protective role for these cells.Two complementary approaches were used to define more carefully the role of CD8+ cells in leishmaniasis.In BALB/c mice susceptible to Leishmania major(L. major)infection, targeted activation of CD8+ cells was attempted by immunization with nonapeptides derived from the conserved major outer surface protein of the organism,8p63, that contained the consensus binding motif for MHC class 1 H-2kd molecules.Two of the nonapeptides induced CTL activity in subsequently infected BALB/c mice that could be elicited against p815 cells pulsed either with peptide or lysates of L.major.purified CD8+ T cells from immunized mice had elevated levels of IFN-γ mRNA transcripts as compared to unimmunized mice.Despite evidence for activation of CD8+ cells,none of the mice immunized with nine different peptides alone or in combination were protected from progressive disease. In a second series of experiments, β2-microglobulin deficient mice that lack CD8+ cells were infected with L. major and the course of infection monitored.These mice cured disease as'rapidly as β2-m +/- and +/+ littermates,and cure was associated with comparable levels of IFN-Y mRNA in the draining lymph node population. Neither of these approaches was able to confirm a substantive role for CD8+ T cells in the primary protective response to L.major.

TARGETEDACTIVATIONOFCD_(8)~(+)CELLSANDINFECTIONOFβ_2-MICROGLOBULIN-DEFICIENTMICE FAILTOCONFIRMAPRIMARYPROTECTIVEROLEFORCD(8)~(+...

 
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