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restriction fragment length polymorphism
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     Restriction Fragment Length Polymorphism in Rice
     水稻DNA限制性片段长度多态性的初步研究
短句来源
     he restriction fragment length polymorphism( RFLP ) of
     采用限制性酶切片段长度多态性分析法,检测在胃癌高发区庄河检出的19例胃癌高系家族成员胃蛋白酶原C基因多态性,发现三种常见片段及一种稀有片段。
短句来源
     The mutations were confirmed by restriction fragment length polymorphism(RFLP).
     针对所发现的突变以DdeⅠ内切酶行限制性片段长度多态性(RFLP)分析。
短句来源
     The mutation was confirmed by restriction fragment length polymorphism (RFLP).
     针对所发现的突变,以限制性内切酶片段长度多态性(RFLP)分析加以验证。
短句来源
     Typing of TTV genomes by restriction fragment length Polymorphism
     TTV基因酶切分型研究
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  restriction fragment length polymorphism
A polymerase chain reaction-restriction fragment length polymorphism analysis was used to discriminate isolates of Bursaphelenchus xylophilus and B.
      
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to genotype GNB3 C825T polymorphism in 354 hypertensive (HT) and 384 normotensive (NT) Uygur subjects.
      
Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis for 13 restriction sites, including BamHI, EcoRV, Sau3AI (one site each), KpnI (two sites), HaeIII (three sites), and RsaI (five sites) were used.
      
A restriction fragment length polymorphism analysis of the IGS2 rDNA region reveals two different AluI profiles, one of which corresponds to Kl.
      
Terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes was used to monitor the changes in the composition of the population of methanogens in enrichment cultures under high and low hydrogen concentrations.
      
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By means of analysing Hind Ⅲ restriction fragments length polymorphism on ,human zeta-globin gene mapping,genotypes of a nondeletion patient and his family were detected,prenatal diagnosis for the fetus at risk for the same disease was successfully made within the first three months of pregnancy The fetus'genotype is (aa/aaT).

利用ζ珠蛋白基因的限制性内切酶HindⅢ特异片段长度多态性分析,鉴别了一个非缺失型HbH病患儿及其父母的单体型,并对该家系正在妊娠中的重型HbH危险胎儿进行了早期产前诊断,确定其基因型为(αα/αα~T)。

Haemophilia A is a commonly inherited disease of blood coagulation. The disorder is caused by deficiency in gene for factor Ⅷ and inherited as an X-linked trait. By use of cloned factor Ⅷ DNA fragment as probe, Bcl Ⅰ restriction fragment length polymorphism (RFLP) located within the factor Ⅷ gene was studied, with which prenatal diagnosis of a case at high risk of haemophilia A was made successfully.

甲型血友病是一种最为常见的遗传性凝血疾病,是由于凝血因子Ⅷ(FⅧ)基因缺陷所致。以克隆的 FⅧ DNA片段为探针对中国人FⅧ基因内的Bcl Ⅰ多态性及其在甲型血友病基因探测中的应用进行研究。利用这一多态性为遗传标志,成功地进行了1例甲型血友病高危胎儿的产前基因诊断。

Restriction fragment length polymorphism (RFLP) of peripheral leucocytes DNA was studied with Southern blot analysis in IDDM paients and controls. IDDM is reportedly associated positively with HLA-DR3 and-DR9, and negatively with DR2 in Chinese.Using HLA-DQβ cDNA probe,an EcoRI 2.2Kb fragment associated with DR2 was found in controls (r = 0.78, P = 0.000001)but not in IDDM patients, with significant difference in frequency(p = 0.02)between these two groups. The frequency of EcoRI 3.0 Kb and BamHI...

Restriction fragment length polymorphism (RFLP) of peripheral leucocytes DNA was studied with Southern blot analysis in IDDM paients and controls. IDDM is reportedly associated positively with HLA-DR3 and-DR9, and negatively with DR2 in Chinese.Using HLA-DQβ cDNA probe,an EcoRI 2.2Kb fragment associated with DR2 was found in controls (r = 0.78, P = 0.000001)but not in IDDM patients, with significant difference in frequency(p = 0.02)between these two groups. The frequency of EcoRI 3.0 Kb and BamHI 3.3 kb fragments are decreased in patient group (p = 0.0032, and p = 0.0018), which has not yet been reported in literature. RFLP analysis suggests that deletion of gene fragment(s) or gene mutations in HLA-D region may have occurred in IDDM patient.

用Southern DNA分析法,对正常人和胰岛素依赖型糖尿病(IDDM)病人外周血白细胞DNA进行限制性片段长度多态性(RFLP)研究。HLA抗原与IDDM相关,我们用HLA-DQβcDNA探针和EcoR Ⅰ、BamH Ⅰ内切酶,测得EcoR Ⅰ 2.2kb片段与正常人DR2抗原相关联(r=0.78,P=1×10~(-6),与IDDM DR2抗原无关联;此片段在DR2的正常组和IDDM组中的频率有显著差异(P=0.02)。EcoR Ⅰ 3.0kb和BamH Ⅰ 3.3kb片段在IDDM组中的频率均降低,与正常组比较其频率有显著差异(P=3.2×10~(-3)和P=1.8×10~(-3),这二片段的差异还未见报道。DNA的RFLP研究提示,IDDM病人中可能是由于基因片段的缺失或是基因结构的改变,导致经酶切后与探针杂交的结果与正常者有差异。

 
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