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at toxin
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  at毒素
     Root length determination results indicated that AT toxin can obviously restrain growth of tobacco seedling root.
     根长测定数据经DPS软件统计分析,结果表明单独施用AT毒素明显抑制烟苗根的生长,差异极显著;
短句来源
     b)Reaction of varieties to pathogen produced AT toxin and tenuazonic acid;
     2.测定各品种对两种Alternaria毒素、AT毒素和细交链格孢酮酸(TA)处理的反应;
短句来源
     AT toxin produced by a virulent strain TBA28 of tobacco brown spot pathogen Alternaria alternata induced systemic resistance being over 80% of a susceptible cultivar NC89. The resistance induced by the toxin was 20% higher than that by a hypovirulent strain TBA16 of the pathogen.
     用赤星病菌 (Alternariaalternata)毒性菌株产生的AT毒素诱导烟草 ,可使寄主获得对赤星病 80 .4 %的系统抗性 ,比病菌弱毒株TBA16孢子诱导的效果高约 2 0 %。
短句来源
     Effects of sodium compound and AT toxin on root length of tobacco seedling
     钠盐与AT毒素对烟苗根生长的影响
短句来源
     A resistant somaclone NC89 TT had been produced by the process of tissue culture and plant regeneration, in which AT toxin was used as pressuring factor. NC89 TT was reproduced by seed production.
     烟草品种NC89心叶叶碟在含AT毒素 60 %和 75%的MS培养基上经 2轮胁迫筛选和植株再生 ,得到抗病品系NC89 TT。
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  “at toxin”译为未确定词的双语例句
     Results Among 8 cell lines,KB and H-4-II-E cell lines showed significant dose-response effect at toxin concentrations greater than 18.8μg/ml with 96-hour incubation.
     结果  8种细胞株中 ,KB细胞和H - 4 -Ⅱ -E细胞在培养 96h ,毒素浓度大于 18 8μg/ml时 ,呈现明显剂量 -反应关系。
短句来源
     The genes of CHS and LOX showed constitutively feeble transcripts in untreated NC89, and were greatly enhanced in their transcript activity in NC89 plants induced by elicitor or AT toxin.
     几丁质酶 (CHT)基因在NC89中组成型表达 ,但受诱导后及在NC89 TT中表达丧失。
短句来源
  相似匹配句对
     Phytopathogenic Toxin
     植物病原毒素
短句来源
     2.toxin.
     2疫毒学说;
短句来源
     Cholera toxin
     霍乱毒素
短句来源
     HEAT-TOXIN IN APOPLEXY
     中风热毒论
短句来源
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  at toxin
The length of this lag phase depends strongly on toxin concentration, extending sharply at toxin levels lower than 60 lethal units (LU) per cell (about one-tenth of the toxin concentration necessary for saturating all surface receptors).
      
Similar values were obtained at toxin concentrations of 4 and 8 nM.
      
These synergistic interactions occurred with IL-1 and IL-6 at toxin concentrations lower than those seen with IL-8.
      
Release of MPO, another component of primary granules, also commenced predominantly at toxin levels ti1 HU/ml.
      
Reaction to AT-toxin is restricted to certain species in the genus Nicotiana.
      
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For improving tobacco resistance to brown spot through somaclonal induction under the pressure of AT-toxin of Alternaria alternata, three cultivars of flue-cured tobacco which are excellent in quality properties but susceptible to the disease in China, were selected as target materials. A native cv, ZY86 ,which is undesirable in quality but more resistant to the disease was used as reference material. The first clones of regenerants were obtained from the toxin-resistant calluses resulted from 2...

For improving tobacco resistance to brown spot through somaclonal induction under the pressure of AT-toxin of Alternaria alternata, three cultivars of flue-cured tobacco which are excellent in quality properties but susceptible to the disease in China, were selected as target materials. A native cv, ZY86 ,which is undesirable in quality but more resistant to the disease was used as reference material. The first clones of regenerants were obtained from the toxin-resistant calluses resulted from 2 selection circulations of AT-toxin stress culture. The second clones of calluses from regenerated plants of first clones showed resistance to AT-toxin at rates of 19% -80% and 14% -69%, respectively. When the toxin contents in media were 60% and 75%. The second clones of regenerated plants occurred at a 100% resistant rate in cv NC89. The best second clone of regenerated plants of NC89 was named as NC89-TT. Seedling from NC89-TT progeny gave 64% and 92% reduction of damage by AT-toxin and infection by spores of the pathogen.

从4个烤烟品种幼苗心叶叶碟产生的愈伤组织(CA)在含AT毒素的培养基上经两轮筛选,获得的抗毒素CA用于产生了抗病的一次再生植株。用来自抗病性提高50%以上的一次再生株的二代CA产生了抗病的二次再生植株,品种NC89的二次再生抗病植株被称为NC89—TT。获得种子后,NC89—TT二代苗显示出抗毒素损伤、抗病菌侵入、抗病斑扩展的能力,与NC89比,毒素引起的根和叶部病变至少晚8小时、轻64%,病菌孢子侵染率低60%以上,病斑面积减小92%。

Two distinct types resistant to brown spot were found in Beinhart1000 1 and in Chinese resistant varieties Jingyehuang and Xujin the Fourth.The resistance of 7 tobacco varieties different in the disease tolerance as determined these were:a)Resistance to invasion and spreading by Alternaria alternata (Fries)Keissler revealed by the number and size of lesions;b)Reaction of varieties to pathogen produced AT toxin and tenuazonic acid;c)Hereditary features by diallel cross analysis.Comparison of the...

Two distinct types resistant to brown spot were found in Beinhart1000 1 and in Chinese resistant varieties Jingyehuang and Xujin the Fourth.The resistance of 7 tobacco varieties different in the disease tolerance as determined these were:a)Resistance to invasion and spreading by Alternaria alternata (Fries)Keissler revealed by the number and size of lesions;b)Reaction of varieties to pathogen produced AT toxin and tenuazonic acid;c)Hereditary features by diallel cross analysis.Comparison of the resistance bearing varieties was based on these determinations.The results showed significant variation of the stated characteristics among the varieties.Brown spot resistance of the selected varieties was found horizontal and polygenic.The Beinhart1000 1 resistance was controlled by dominant genes,expressed as resistance to invasion with fewer lesions,but the average size of the lesions was large.On the other hand,resistant genes of the Chinese varieties were mostly recessive,showing tolerance to both invasion and spreading in expression.Varietal sensitivity to AT toxin and tenuazonic acid had significant correlations separately with invasion and spreading susceptibility to the fungus.Roles of the fungal toxins in pathogenesis and their use in research concerning disease resistance are discussed.The possible applicatien of the differently characterized resistance sources to tobacco breeding is proposed.

国外赤星病抗源Beinhart1000-1和国内抗病品种净叶黄及许金4号的抗病性分属两种不同的类型。本文对7个赤星病抗感程度不同的烟草品种的抗病性分别进行了以下测定:1.通过观察病斑数量和病斑大小测定抗侵入和抗扩展能力;2.测定各品种对两种Alternaria毒素、AT毒素和细交链格孢酮酸(TA)处理的反应;3.双列杂交法分析抗性遗传规律。重点对Bein-hart1000-1和净叶黄的抗性特点进行比较。结果表明:供试品种的抗性特点差异显著,为多基因控制的水平抗性。Beinhart1000-1的抗病性是由显性基因控制的,主要表现为抗侵入,病斑数少,但病斑面积却显著大于净叶黄和许金4号。国内抗病品种抗扩展的能力强,同时也较抗侵入,它们的抗性基因大部分是隐性的。各品种对AT毒素和TA的敏感程度分别与其抗病菌侵入和抗病斑扩展的能力高度相关。文中还就真菌毒素在致病过程中的作用以及它们在赤星病抗性研究中的应用做了讨论。同时,就不同抗性类型的抗源在烟草抗病育种中应用的可能性进行了分析

AT toxin produced by a virulent strain TBA28 of tobacco brown spot pathogen Alternaria alternata induced systemic resistance being over 80% of a susceptible cultivar NC89. The resistance induced by the toxin was 20% higher than that by a hypovirulent strain TBA16 of the pathogen. A resistant somaclone NC89 TT had been produced by the process of tissue culture and plant regeneration, in which AT toxin was used as pressuring factor. NC89 TT was reproduced by seed production. Expressions of...

AT toxin produced by a virulent strain TBA28 of tobacco brown spot pathogen Alternaria alternata induced systemic resistance being over 80% of a susceptible cultivar NC89. The resistance induced by the toxin was 20% higher than that by a hypovirulent strain TBA16 of the pathogen. A resistant somaclone NC89 TT had been produced by the process of tissue culture and plant regeneration, in which AT toxin was used as pressuring factor. NC89 TT was reproduced by seed production. Expressions of 5 defence genes were tested and DNA structural alternations were analyzed. Dot blots of RNAs from NC89 and NC89 TT plants either immunized by the elicitor and AT toxin or untreated were probed with cDNAs for pathogensis related (PR) protein PR 1a, chitinase (CHT), phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), and lipoxygenase (LOX). Both PAL and PR la genes did not constitutively transcript in untreated NC89, and did in the fourth generation plants of NC89 TT. While, CHT gene was inverse. Transcription of PR la gene was activated by elicitor or AT toxin, and transcription of PAL gene only by the toxin .The genes of CHS and LOX showed constitutively feeble transcripts in untreated NC89, and were greatly enhanced in their transcript activity in NC89 plants induced by elicitor or AT toxin. A consistent tendency was found between the transcription activity and product accumulation of the genes indicated by PR la electrophoresis. Genome DNA structure mutation in different tobaccos was tested by random amplified polymorphic DNA (RAPD) using 5 primers. It was suggested that the sequence rearrangement or nucleotide substitution was responsible for the constitutive expression of the defence genes and such an expression resulted in resistance enhancement.

用赤星病菌 (Alternariaalternata)毒性菌株产生的AT毒素诱导烟草 ,可使寄主获得对赤星病 80 .4 %的系统抗性 ,比病菌弱毒株TBA16孢子诱导的效果高约 2 0 %。烟草品种NC89心叶叶碟在含AT毒素 60 %和 75%的MS培养基上经 2轮胁迫筛选和植株再生 ,得到抗病品系NC89 TT。用 5种防卫基因的cDNA探针检测了不诱导、受AT毒素诱导、受病菌弱毒株激发子诱导的NC89和诱导与不诱导的NC89 TT第 4代植株 5种基因的转录活性 ,结果表明 ,苯丙氨酸氨裂解酶(PAL)基因在NC89中不能组成型表达 ,可被AT毒素诱导激活 ,但不受激发子诱导 ;病程相关蛋白(PR蛋白 )PR 1a、查尔酮合成酶 (CHS)、脂氧合酶 (LOX)基因在NC89中表现为诱导转录或诱导后转录活性增强 ;这 4种基因都能在NC89 TT中组成型表达。几丁质酶 (CHT)基因在NC89中组成型表达 ,但受诱导后及在NC89 TT中表达丧失。烟草基因组DNA的随机引物扩增 (RAPD)测定表明 ,AT毒素诱导了DNA结构的明显变化 ;与亲本NC89相比 ,NC89 TT的基因组DNA显示明显的序...

用赤星病菌 (Alternariaalternata)毒性菌株产生的AT毒素诱导烟草 ,可使寄主获得对赤星病 80 .4 %的系统抗性 ,比病菌弱毒株TBA16孢子诱导的效果高约 2 0 %。烟草品种NC89心叶叶碟在含AT毒素 60 %和 75%的MS培养基上经 2轮胁迫筛选和植株再生 ,得到抗病品系NC89 TT。用 5种防卫基因的cDNA探针检测了不诱导、受AT毒素诱导、受病菌弱毒株激发子诱导的NC89和诱导与不诱导的NC89 TT第 4代植株 5种基因的转录活性 ,结果表明 ,苯丙氨酸氨裂解酶(PAL)基因在NC89中不能组成型表达 ,可被AT毒素诱导激活 ,但不受激发子诱导 ;病程相关蛋白(PR蛋白 )PR 1a、查尔酮合成酶 (CHS)、脂氧合酶 (LOX)基因在NC89中表现为诱导转录或诱导后转录活性增强 ;这 4种基因都能在NC89 TT中组成型表达。几丁质酶 (CHT)基因在NC89中组成型表达 ,但受诱导后及在NC89 TT中表达丧失。烟草基因组DNA的随机引物扩增 (RAPD)测定表明 ,AT毒素诱导了DNA结构的明显变化 ;与亲本NC89相比 ,NC89 TT的基因组DNA显示明显的序列重排或核苷酸替换。

 
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