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   clone and sequencing 的翻译结果: 查询用时:0.194秒
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clone and sequencing
相关语句
  克隆测序
     By clone and sequencing, the sequences of 12 TDFs were obtained and 3 of them are all strongly induced and have same length and have the identity of 99%.
     经过克隆测序,最终获得了12个BABA诱导上调基因片断的序列,其中有3个差异明显的TDFs的序列长度一致,序列同源性达99%。
短句来源
     After identification of T/A clone and sequencing, the chymotrypsin gene was subcloned into the eukaryotic expression vector pIE1-3, and so the recombinant expression vector of insect cell (pIE1-3/chy) was constructed. Identifications of restriction digestion and PCR were made before the above recombinant vector was co-transfected into Aedes albopictus C6/36 cell with pIE1-neo plasmid.
     经T/A克隆测序鉴定后,亚克隆到真核表达载体pIE13,构建重组昆虫细胞表达载体pIE13/chy,经酶切和PCR鉴定后,与带有筛选标记的pIE1neo质粒共转染蚊细胞C6/36,通过G418选择培养,建立转染细胞系;
短句来源
     After identification of T/A clone and sequencing, the trypsin gene was subcloned into the eukaryotic expression vector pIE1-3, and the recombinant expression vector of insect cell (pIE1-3/try) was constructed.
     T/A克隆测序鉴定后,亚克隆到昆虫细胞表达载体pIE13,构建重组昆虫细胞表达载体pIE13/try。
短句来源
     However, which NMPs expressed differentially in EC remains unknown. . Base on this, the objective of this study was to separate and identify the differentially expressed NMPs between SHEE and SHEEC by 2-DE, MALDI-TOF- MS, RT-PCR, cDNA clone and sequencing analysis, which may contribute to further study the pathogenesis of esophageal carcinoma.
     为此,本研究联合运用双向电泳、基质辅助激光解吸附飞行时间质谱(MALDI-TOF-MS)、RT-PCR、cDNA克隆测序和生物信息学分析等技术手段对食管癌细胞差异表达核基质蛋白进行了研究,以期为进一步研究食管癌的发病机制提供新线索。
短句来源
     The coding genes of carbapenamases and the gene environments were investigate by PCR, clone, and sequencing.
     PCR扩增及克隆测序分析碳青霉烯酶基因及其周围基因环境。
  “clone and sequencing”译为未确定词的双语例句
     Clone and Sequencing of gD and gE Gene of PRV XIN-W Isolate of Pseudorabies virus
     猪伪狂犬病病毒XIN-W株gD和gE基因的克隆及序列分析
短句来源
     Clone and Sequencing of gp37 of Subgroup J Avian Leukosis Viruses in China
     J亚群禽白血病病毒gp37基因的克隆和序列分析
短句来源
     Method:The mutation and deletion of exon5,exon6 and exon8 of PTEN gene were detected by PCR, gene clone and sequencing in mucoepidermoid carcinoma of parotid gland and normal gland tissue.
     方法 :应用聚合酶链反应(PCR)及克隆、基因测序的方法来分析腮腺粘液表皮样癌及正常腺体组织中PTEN基因第 5、6、8外显子有无突变及缺失。
短句来源
     Based on molecular clone and sequencing analysis on one unique copy of nrDNA ITS region in Nothotsuga longibracteata,the structural characteristics of pseudogene were studied. Moreover,we discussed the molecular mechanism of pseudogenization.
     以长苞铁杉(Nothotsuga longibracteata)为实验材料,通过nrDNA ITS区特异拷贝的克隆及假基因化分析,在序列水平上探讨假基因化的特征,进而探讨引起多拷贝基因家族假基因化的分子机制。
短句来源
     Methods:The mutation and deletion of exon5,exon6 and exon8 of PTEN gene were detected by PCR, gene clone and sequencing in OSCC and normal oral mucous membrane.
     方法 :应用聚合酶链反应(PCR)及克隆、基因测序的方法来分析口腔颌面部鳞状细胞癌及正常黏膜组织中PTEN基因第 5、第 6及第 8外显子有无突变及缺失。
短句来源
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  相似匹配句对
     Clone
     克隆
短句来源
     The typing results were compared with the sequencing of clone.
     再从30种不明确杂合子类型中各挑2个样品进行克隆测序,结果与SSP分型结果比较。
短句来源
     The sequencing result confirms the success of e-clone.
     设计引物,进行PCR,测序结果与电子克隆结果完全一致,证明克隆成功。
短句来源
     Clone and Sequencing of Vitellogenin in Antheraea yamamai
     天蚕卵黄原蛋白cDNA的克隆及序列分析
短句来源
     Undertake TA clone with the production of PCR and sequencing.
     将PCR产物行TA克隆进行测序。
短句来源
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  clone and sequencing
Primer extension analysis suggested that cD4H-3, the longest cDNA clone, was missing 156 bp at the 5' end of the clone and sequencing of the genomic clone, gD4H-8, confirmed these results.
      


In studying the gene structure of ginsenoside β glycosidase,we have designed RT PCR primer according to the N terminal sequence of amino acid, Using CsCl density gradient centrifugation,we have extracted total RNA of sp.48?g.RT PCR was accomplished and the specific amplication band of 1.0?kb was obtained. DNA clone and sequencing are also accomplished. It is found in the Internet that there has nohomogeneity with any known gene and it may be a novel gene of ginsenoside β glycosidase.

为了研究人参皂甙 β 糖苷酶的基因结构 ,根据人参皂甙 β 糖苷酶的氨基酸N 末端序列设计了RT PCR引物 ,应用氯化铯密度梯度离心法提取了菌体总RNA ,以JFX0 1为模板进行RT PCR反应得到一条 1.0kb的基因 ,并对该基因进行了克隆测序。该测序结果与已知蛋白的基因无同源性 ,说明所得到的人参皂甙 β 糖苷酶的基因很有可能是新的基因

Objective:To investigate the role of anti-oncogene PTEN in the tumorigenesis and development of oral squamous cell carcinoma (OSCC).Methods:The mutation and deletion of exon5,exon6 and exon8 of PTEN gene were detected by PCR, gene clone and sequencing in OSCC and normal oral mucous membrane.Results:There was no mutation and deletion in exon5,exon6 and exon8 of PTEN gene in OSCC and normal oral mucous membrane after gene sequencing.Conclusions:At the level of DNA , anti-oncogene PTEN do not play...

Objective:To investigate the role of anti-oncogene PTEN in the tumorigenesis and development of oral squamous cell carcinoma (OSCC).Methods:The mutation and deletion of exon5,exon6 and exon8 of PTEN gene were detected by PCR, gene clone and sequencing in OSCC and normal oral mucous membrane.Results:There was no mutation and deletion in exon5,exon6 and exon8 of PTEN gene in OSCC and normal oral mucous membrane after gene sequencing.Conclusions:At the level of DNA , anti-oncogene PTEN do not play any roles in OSCC.

目的 :探讨抑癌基因PTEN在口腔颌面部鳞状细胞癌发生、发展过程中的作用。方法 :应用聚合酶链反应(PCR)及克隆、基因测序的方法来分析口腔颌面部鳞状细胞癌及正常黏膜组织中PTEN基因第 5、第 6及第 8外显子有无突变及缺失。结果 :通过对PTEN基因三个外显子的克隆测序 ,均未发现有突变及缺失。结论 :在DNA水平抑癌基因PTEN在口腔鳞状细胞癌的发生过程中不起作用

Objective:To investigate the role of anti-oncogene PTEN in the tumorigenesis and development of mucoepidermoid carcinoma of parotid gland.Method:The mutation and deletion of exon5,exon6 and exon8 of PTEN gene were detected by PCR, gene clone and sequencing in mucoepidermoid carcinoma of parotid gland and normal gland tissue.Result:There was no mutation and deletion in exon5,exon6 and exon8 of PTEN gene in mucoepidermoid carcinoma of parotid gland and normal gland tissue after geoe sequencing.Conclusion:At...

Objective:To investigate the role of anti-oncogene PTEN in the tumorigenesis and development of mucoepidermoid carcinoma of parotid gland.Method:The mutation and deletion of exon5,exon6 and exon8 of PTEN gene were detected by PCR, gene clone and sequencing in mucoepidermoid carcinoma of parotid gland and normal gland tissue.Result:There was no mutation and deletion in exon5,exon6 and exon8 of PTEN gene in mucoepidermoid carcinoma of parotid gland and normal gland tissue after geoe sequencing.Conclusion:At the level of DNA , anti-oncogene PTEN do not play any roles in mucoepidermoid carcinoma of parotid gland.

目的 :探讨抑癌基因PTEN在腮腺粘液表皮样癌发生、发展过程中的作用。方法 :应用聚合酶链反应(PCR)及克隆、基因测序的方法来分析腮腺粘液表皮样癌及正常腺体组织中PTEN基因第 5、6、8外显子有无突变及缺失。结果 :通过对PTEN基因 3个外显子的克隆测序 ,均未发现有突变及缺失。结论 :初步推断 ,在DNA水平抑癌基因PTEN在腮腺粘液表皮样癌的发生过程中不起作用

 
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