助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   cultural human 在 眼科与耳鼻咽喉科 分类中 的翻译结果: 查询用时:0.451秒
图标索引 在分类学科中查询
所有学科
眼科与耳鼻咽喉科
更多类别查询

图标索引 历史查询
 

cultural human
相关语句
  培养人
    Construction and expression of eukaryotic fluorescent expressing vector of human MRG15 gene in cultural human lens epithelial cells
    人MRG15基因荧光真核表达载体的构建及其在培养人晶状体上皮细胞的表达
短句来源
    Objective To clone the coding sequence of human MRG15 (MORF4-related gene on chromosome 15), construct and express its eukaryotic fluorescent expressing vector in cultural human lens epithelial cells, for the investigation of the relationship between MRG15 and age related cataract (ARC).
    目的克隆人MRG15(MORF4-related-gene on chromosome15)的编码基因,构建荧光表达载体,并检测其在培养人晶状体上皮细胞中的表达,为研究MRG15与年龄相关性白内障(age related cataract,ARC)的相关性奠定基础。
短句来源
    Conclusion Human MRG15 coding region, cloned from anterior lens capsule of ARC,was successfully constructed into pEGFP-N2,and can be expressed in cultural human lens epithelial cells.
    结论克隆了人MRG15的编码基因,成功构建了荧光真核表达载体MRG15-pEGP-N2,并可在培养人晶状体上皮细胞中表达。
短句来源
    Results Human MRG15 was successfully amplified from anterior lens capsule of ARC,and expected fragment was digested from the recombined vector, MRG15-pEGFP-N2. It was observed that MRG15 was localized within the nucleus of cultural human lens epithelial cells 24 hours after transfection.
    重组质粒MRG15-pEGP-N2经酶切后产生与理论预期长度相符的片段。 脂质体法转染后24h,观察到其主要在培养人晶状体上皮细胞的细胞核中表达。
短句来源
  “cultural human”译为未确定词的双语例句
    Objective:To investigate the cytotoxicity of cobra venom factor(CVF),immunoconjugate(BAC5-CVF),and direct lytic fator(DLF)on cultural human nasopharyngeal tumor cell line(CNE2)and the synergism of DLF on antitumor drug cytotoxicity.
    目的 :探讨纯化的眼镜蛇毒因子 (CVF) ,CVF和单抗交联物 (BAC5 -CVF)和直接溶解因子 (DLF)对人鼻咽癌细胞株的毒性及DLF和抗癌药的相互作用。
短句来源
    Cytotoxicity of cobra venom lytic factors on cultural human nasopha ryngeal tumor cell line and its synergism with antitumor drugs
    眼镜蛇毒溶解因子对人鼻咽癌细胞株的毒性及其与抗癌药的协同作用
短句来源
    We firstly report an experimental study on the sensitivity of primary cultural human trigeminal ganglionic cells infected by herpes simplex virus type l(HSV-l) in different time-points.
    首次报告了人三叉神经节Trigeminal Ganglian(TG)三种原代培养细胞在体外对单纯疱疹病毒1型(HSV-1)Mckrae株感染后的不同时相的敏感性实验研究。
短句来源
查询“cultural human”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  cultural human
BOOK REVIEW: LIVES ACROSS CULTURES: CROSS-CULTURAL HUMAN DEVELOPMENT.
      
Objective: To explore the effects of matrine on HCCR1 and HCCR2 expression in cultural human hepatocellular carcinomas (HCC) cells at the level of gene and protein.
      
Effect of matrine on expression of HCCR1 and HCCR2 proteins in cultural human hepatocellular carcinomas cells
      
The article also makes links to broader issues such as the racial, ethnic, linguistic and cultural human rights instruments, as well as to the important linkage to international poverty law.
      
They will examine the basic assumptions and issues of cross-cultural human relations.
      
更多          


We firstly report an experimental study on the sensitivity of primary cultural human trigeminal ganglionic cells infected by herpes simplex virus type l(HSV-l) in different time-points. The results indicate that the neuron of human trigeminal ganglia is the most sensitive cell type, the antigen of HSV-1 replicates rapidly within the cytoplasm and cell nucleus, the fibroblasts and glia cells are infected partly by the virus. Therefore, perhaps the neuron is the main harboring cell type. Eye Science...

We firstly report an experimental study on the sensitivity of primary cultural human trigeminal ganglionic cells infected by herpes simplex virus type l(HSV-l) in different time-points. The results indicate that the neuron of human trigeminal ganglia is the most sensitive cell type, the antigen of HSV-1 replicates rapidly within the cytoplasm and cell nucleus, the fibroblasts and glia cells are infected partly by the virus. Therefore, perhaps the neuron is the main harboring cell type. Eye Science

首次报告了人三叉神经节Trigeminal Ganglian(TG)三种原代培养细胞在体外对单纯疱疹病毒1型(HSV-1)Mckrae株感染后的不同时相的敏感性实验研究。结果表明人TG中的神经节细胞对HSV-1感染最敏感,HSV—1抗原可以在其胞浆及胞核中迅速出现。成纤维细胞和胶质细胞也有部分感染现象。这种结果提示TG中的神经节细胞很可能是HSV-1主要的潜伏感染细胞。

Objective:To investigate the cytotoxicity of cobra venom factor(CVF),immunoconjugate(BAC5-CVF),and direct lytic fator(DLF)on cultural human nasopharyngeal tumor cell line(CNE2)and the synergism of DLF on antitumor drug cytotoxicity. Methods:The cytotoxicity was detected using MTT method or typanblue exclusion test,the enhancement was confirmed with the combination of DLF in the level inducing 20%growth inhibition and antitumor drugs in the level inducing 50%growth inhibition of CNE2 cells. Results:No any...

Objective:To investigate the cytotoxicity of cobra venom factor(CVF),immunoconjugate(BAC5-CVF),and direct lytic fator(DLF)on cultural human nasopharyngeal tumor cell line(CNE2)and the synergism of DLF on antitumor drug cytotoxicity. Methods:The cytotoxicity was detected using MTT method or typanblue exclusion test,the enhancement was confirmed with the combination of DLF in the level inducing 20%growth inhibition and antitumor drugs in the level inducing 50%growth inhibition of CNE2 cells. Results:No any cytoxicity of CVF itself on CNE2 cells was found,but BAC5-CVF can induce cytotoxicity on the same cell line in dose dependent manner,the IC50 is 3 07×10 -7 mol/L.DLF can enhance cytotoxicity of conventional antitumor drugs including VLB,MTX,ADM,5-FU,and BLM,but antagonize that of HN2. Conclusions:Both BAC5-CVF and DLF have cytotoxicity on CNE2 cells,and DLF can enhance cytotoxicity of the conventional antitumor drugs on cultural CNE2 cells except NH2.

目的 :探讨纯化的眼镜蛇毒因子 (CVF) ,CVF和单抗交联物 (BAC5 -CVF)和直接溶解因子 (DLF)对人鼻咽癌细胞株的毒性及DLF和抗癌药的相互作用。方法 :用MTT法或台盼蓝拒染法了解合并用药的细胞毒作用。结果 :CVF本身对CNE2细胞并无细胞毒性 ;BAC5 -CVF对CNE2细胞有剂量依赖性杀伤作用 ,其IC5 0为 3 0 7× 10 -7mol/L ;引起 2 2 0 3 %抑制效应的DLE与长春碱 (VLB)、甲氨蝶呤 (MTX)、阿霉素 (ADM)、氟尿嘧啶 ( 5 -FU)和平阳霉素 (BLM)有协同作用 ,但能拮抗氮芥 (HN2 )作用。结论 :CVF不引起CNE2细胞毒性 ,但BAC5 -CVF和DLE对CNE2细胞有毒性作用 ;除能拮抗氮芥外 ,DLE和其它抗癌药对人鼻咽癌细胞株细胞毒性有协同作用

In order to isolate and screem tissue specific genes of human nasopharynx and new tumor suppressor genes of nasopharyngeal carcinoma(NPC),a directional cDNA library from human embryo nasopharyngeal epithelia was constructed by SMART(switching mechanism at 5′ end of RNA transcript) technique. The total RNA and mRNA were separated from primary cultural human embryo nasopharyngeal epithelia and the frist strand cDNA was synthesized through reverse transcription by a modified oligo(dT) primer(contained ...

In order to isolate and screem tissue specific genes of human nasopharynx and new tumor suppressor genes of nasopharyngeal carcinoma(NPC),a directional cDNA library from human embryo nasopharyngeal epithelia was constructed by SMART(switching mechanism at 5′ end of RNA transcript) technique. The total RNA and mRNA were separated from primary cultural human embryo nasopharyngeal epithelia and the frist strand cDNA was synthesized through reverse transcription by a modified oligo(dT) primer(contained sfi ⅠB site) while the SMART oligonucleotide (contained sfi ⅠA site) was utilized as a template so that the first strand cDNA could be extended over the 5′end of mRNA. The double strand cDNA was amplified by LD PCR(long distance PCR) with the above two primers and then digested by sfi Ⅰ(ⅠA & ⅠB) restriction enzyme.After cDNA size fractionation through CHROMASPIN column,the double strand cDNA was ligated into the sfi Ⅰ digested λTripIEx2 vector and then the recombinant DNA was packaged in vitro . The unamplified human embryo nasopharynx cDNA library consists of 1 0×10 6 independent clones in which the percentage of recombinant clones is about 96%.The titer of the amplified cDNA library is 7 8×10 9 pfu/ml and the average exogenous inserts of the recombinants is 1 2 kb.The full length cDNA of NAG4, a candidate tumor suppressor gene related with nasopharyngeal carcinoma, was amplified in the cDNA library by PCR. These results show that the human embryo nasopharynx cDNA library has an excellent quality and lays solid foundation for screening and cloning new tumor suppressor genes of nasopharyngeal carcinoma and tissue specific genes of human nasopharynx.

为了进一步分离人鼻咽组织特异性表达基因和鼻咽癌特异相关基因 ,采用SMART (switchingmechanismat 5′endofRNAtranscript)技术 ,构建了人胚鼻咽上皮cDNA文库 .从原代培养的人胚鼻咽上皮分离总RNA并纯化mRNA ,利用经修饰的oligo (dT)引物 (含sfiⅠB酶切位点 )合成cDNA第一链 ,同时根据真核生物mRNA5′端帽子结构特点 ,利用SMART核苷酸 (含sfiⅠA酶切位点 )作为cDNA第一链在mRNA 5′端延伸出去的模板 ,进而以此序列为引物利用LD PCR (long distance PCR)合成双链cDNA ,双链cDNA经sfiⅠ (ⅠA和ⅠB)酶切和过柱分级分离后 ,克隆入经sfiⅠ酶切的λTrip1EX2载体后经体外包装而成cDNA文库 .结果表明 ,原始人胚鼻咽上皮cDNA文库获得 1 0× 10 6个重组子 ,重组率达 96 % .文库扩增后 ,滴度达 7 8× 10 9pfu ml,插入cDNA平均长度为 1 2kb ,用PCR从该文库扩增出本实验室新克隆的鼻咽癌相关基因NAG4的全长cDNA .构建的人胚鼻咽上皮cDNA文库...

为了进一步分离人鼻咽组织特异性表达基因和鼻咽癌特异相关基因 ,采用SMART (switchingmechanismat 5′endofRNAtranscript)技术 ,构建了人胚鼻咽上皮cDNA文库 .从原代培养的人胚鼻咽上皮分离总RNA并纯化mRNA ,利用经修饰的oligo (dT)引物 (含sfiⅠB酶切位点 )合成cDNA第一链 ,同时根据真核生物mRNA5′端帽子结构特点 ,利用SMART核苷酸 (含sfiⅠA酶切位点 )作为cDNA第一链在mRNA 5′端延伸出去的模板 ,进而以此序列为引物利用LD PCR (long distance PCR)合成双链cDNA ,双链cDNA经sfiⅠ (ⅠA和ⅠB)酶切和过柱分级分离后 ,克隆入经sfiⅠ酶切的λTrip1EX2载体后经体外包装而成cDNA文库 .结果表明 ,原始人胚鼻咽上皮cDNA文库获得 1 0× 10 6个重组子 ,重组率达 96 % .文库扩增后 ,滴度达 7 8× 10 9pfu ml,插入cDNA平均长度为 1 2kb ,用PCR从该文库扩增出本实验室新克隆的鼻咽癌相关基因NAG4的全长cDNA .构建的人胚鼻咽上皮cDNA文库具有良好的质量 ,该cDNA文库为进一步筛选、克隆鼻咽癌抑癌基因及鼻咽组织特异性表达基因奠定了基础

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关cultural human的内容
在知识搜索中查有关cultural human的内容
在数字搜索中查有关cultural human的内容
在概念知识元中查有关cultural human的内容
在学术趋势中查有关cultural human的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社