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tf protein
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  tf蛋白
     VEGF and TF protein level in tumor tissue were detected by Western blot assay.
     应用Western blot方法检查肿瘤组织VEGF和TF蛋白表达。
短句来源
     Results showed that TF protein content and mRNA level were increased when ECV304 exposed to mmLDL(40mg/L)for 4 h.
     结果显示 ,4 0mg LmmLDL作用ECV30 4 4h ,能使TF蛋白含量及mRNA水平显著增加 ,并迅速升高 [Ca2 + ]i。
短句来源
     TF protein content was measured by ELISA assay. The TF mRNA was detected with RT PCR method. [Ca 2+ ] i in single ECV304 cell was observed with Laser Confocal Microscope.
     采用ELISA法检测细胞内TF蛋白含量 ,用RT PCR法检测细胞内TFmRNA水平 ,用激光共聚焦显微镜观察 [Ca2 + ]i 的变化。
短句来源
     Methods Platelets were isolated with Sepharose 2B gel column. ELISA was employed to detect the TF protein content in the lysates of washed platelets,procoagulant activity of platelet-associated TF was measured with one stage clotting time assay.
     方法采用Sepharose2B凝胶柱分离纯化血小板,ELISA法测定洗涤血小板破碎液TF蛋白含量;
短句来源
     RESULTS: Coincubation with macrophage membrane (cell ratio 1∶1) for 6 hours resulted in a 10.9-fold increase ( P< 0 01) in ECs procoagulant activity (PCA) as assayed by 1-step recalcification clotting time of intact cells. A significant increase in TF protein expression was demonstrated by Western blot.
     结果 :与巨噬细胞膜共孵育 6h后 ,内皮细胞促凝活性增强 1 0 9倍(P <0 0 1 ) ,TF蛋白的表达亦显著增强。
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  因子蛋白
     10 -6mol/L angiotensin Ⅱ induced TF protein levels and mRNA expression apparently, while co-incubation with 17β-estradiol and α-zearalanol might reverse this change.
     结果发现 :10 -7mol L 17β E2 及α ZAL均可明显降低HUVECs组织因子蛋白含量及mRNA的表达 ,二者的作用类似。
短句来源
     Meth ods The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antis ense TFc DNA were transfected into LOVO cells by lipofactamine 2000. TF protein expression in the transfected cells was detected by Western blot.
     方法利用构建有正义/反义组织因子cDNA(TFcDNA)的真核表达载体pcDNA3.1/Zeo,以脂质体法转染LOVO细胞,采用Western印迹分析检测转染成功LOVO细胞的组织因子蛋白表达水平。
短句来源
  “tf protein”译为未确定词的双语例句
     After treatment by mmLDL, [Ca 2+ ] i in single ECV304 increased distinctly, which was inhibited by ginkgolides B. The increase of TF protein level and mRNA expression in HUVECs was induced by mmLDL, and the PKC route and [Ca 2+ ] i may be important in the concern mechanisms.
     以上结果提示 ,mmLDL可诱导ECV30 4TF表达 ,Ca2 + 与PKC参与mmLDL诱导ECV30 4TF基因表达过程 ,并且银杏内酯B可抑制mmLDL的此种效应
短句来源
     The results showed that the expression of PCA, TF protein and its activity in PBMNC increased significantly after being stimulated by rhIL-6(P<0.01).
     结果表明:rhIL6培养PBMNC的PCA、TF表达及活性均较对照组显著增加(P<0.01);
短句来源
     This effect was associated with reduction of TF protein expression and activity by rhIL-10(P<0.01).
     在500ng/LrhIL10的作用下,rhIL6诱导的PBMNC的PCA、TF表达和活性均显著降低(P<0.01)。
短句来源
     Results: Stimulation of HUVECs with 10-6mol/L Ang Ⅱ under basal conditions resulted in a drastic increase in TF mRNA and TF protein. Application of 17β-estradiol at physiological level (10-9mol/L) significantly attenuated 10-6 mol/L Ang Ⅱ-induced increase of TF expression at both mRNA and protein level, while with increasing dose, its inhibition of TF expression weakened gradually.
     结果 10-6mol/L Ang Ⅱ对HUVECs有明显的损伤作用,内皮细胞TF mRNA表达和蛋白合成均显著增加,10-9mol/L17β-E2明显抑制Ang Ⅱ对内皮细胞TF mRNA表达和蛋白合成的刺激作用,但随着雌激素浓度的增加,抑制作用逐渐减弱;
短句来源
     In ASODN group,VEGF protein expression was lower,but TF protein expression was higher.
     ASODN组肿瘤组织中VEGF蛋白表达较弱,而TF表达较强。
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  tf protein
In all species, the alternative transcript does not contain the signal peptide sequence and possibly encode for a Tf protein devoid of signal peptide showing that this phenomenon is not restricted to human gene.
      
This nucleotide substitution causes a nonconservative amino acid change from glutamate to lysine in amino acid residue 375 of the TF protein.
      
By an isoelectric focusing analysis, we found that the patient and his father shared a variant TF protein with an abnormal isoelectric point.
      
TF mRNA was detected in some foamy macrophages within the thrombus, TF protein was localized to the rough endoplasmic reticulum and plasma membrane of many macrophages, some fibroblasts, and a few endocardial cells.
      
In rats with NBTE, TF protein was present in foamy macrophages and spindle stromal cells and focally present in the extracellular matrix.
      
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To study the effects of mmLDL and ginkgolides B on the expression of protein content and mRNA lever of tissue factor and intracellular free calcium concentration ([Ca 2+ ] i ) in ECV304 endothelial cells. TF protein content was measured by ELISA assay. The TF mRNA was detected with RT PCR method. [Ca 2+ ] i in single ECV304 cell was observed with Laser Confocal Microscope. Results showed that TF protein content and mRNA level were increased when ECV304 exposed to mmLDL(40mg/L)for...

To study the effects of mmLDL and ginkgolides B on the expression of protein content and mRNA lever of tissue factor and intracellular free calcium concentration ([Ca 2+ ] i ) in ECV304 endothelial cells. TF protein content was measured by ELISA assay. The TF mRNA was detected with RT PCR method. [Ca 2+ ] i in single ECV304 cell was observed with Laser Confocal Microscope. Results showed that TF protein content and mRNA level were increased when ECV304 exposed to mmLDL(40mg/L)for 4 h. Staurosporine(400nmol/L), the protein kinase C (PKC) inhibitor, and ginkgolides B(40mg/L) markedly inhibited TF protein content and mRNA expression induced by mmLDL. After treatment by mmLDL, [Ca 2+ ] i in single ECV304 increased distinctly, which was inhibited by ginkgolides B. The increase of TF protein level and mRNA expression in HUVECs was induced by mmLDL, and the PKC route and [Ca 2+ ] i may be important in the concern mechanisms.

为研究弱氧化修饰低密度脂蛋白 (mmLDL)对ECV30 4组织因子 (TF)蛋白含量和mRNA水平的影响、对胞浆游离钙离子 ([Ca2 + ]i)的影响 ,以及银杏内酯B等的干预作用。采用ELISA法检测细胞内TF蛋白含量 ,用RT PCR法检测细胞内TFmRNA水平 ,用激光共聚焦显微镜观察 [Ca2 + ]i 的变化。结果显示 ,4 0mg LmmLDL作用ECV30 4 4h ,能使TF蛋白含量及mRNA水平显著增加 ,并迅速升高 [Ca2 + ]i。抗氧化剂银杏内酯B及PKC抑制剂Staurosporine可拮抗mmLDL的上述诱导反应。以上结果提示 ,mmLDL可诱导ECV30 4TF表达 ,Ca2 + 与PKC参与mmLDL诱导ECV30 4TF基因表达过程 ,并且银杏内酯B可抑制mmLDL的此种效应

Adopting the vertical plate, unsuccessful polypropyleneamide gel electuophoresis method, exam the diversity of black feather strain Tulufan chicken's Tf protein can be seperated to 6~9 tape, controlled by A, B, C, these three common dominant genes, it's usual expression are AB and AC, and have the difference of male or female, which are richer in Tf genes storage than local wild chicken \;

用垂直板不连续聚丙烯酰胺凝胶电泳法测定了黑羽品系吐鲁番鸡血清转铁蛋白的多态性。结果表明 :黑羽品系吐鲁番鸡转铁蛋白可以分离出 6~ 9条带 ,受 A,B,C三个共显性等位基因的控制 ,常见表型 AB和 AC型 ,并且雄性与雌性之间有差异。比当地野生型石鸡血清转铁蛋白基因储备丰富

Objective :To analyze the role of tissue factor (TF) in the in vitro invasive ability of human colon carcinoma cells (HT 29). Methods: The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT 29 cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western blot. In vitro Matrigel invasion assays were used to show the invasive ability of HT 29 cells with sense/antisense TFcDNA transfection. Results:...

Objective :To analyze the role of tissue factor (TF) in the in vitro invasive ability of human colon carcinoma cells (HT 29). Methods: The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT 29 cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western blot. In vitro Matrigel invasion assays were used to show the invasive ability of HT 29 cells with sense/antisense TFcDNA transfection. Results: HT 29 cells with sense TFcDNA transfection showed increased TF expression and invasive ability compared with the cells without transfection, but HT 29 cells with antisense TFcDNA transfection got the contrary change. Conclusion: TF can increase the invasive ability of HT 29 cells in vitro.

目的 :分析组织因子表达对人类大肠癌细胞 (HT 2 9细胞 )体外侵袭能力的影响。方法 :利用构建有正义/反义组织因子cDNA(TFcDNA)的真核表达载体 pcDNA3.1 /Zeo ,以脂质体法转染HT 2 9细胞 ,经验证转染成功的HT 2 9细胞采用Western印迹分析检测组织因子的蛋白表达水平并进行基质胶 (Matrigel)侵袭实验观察细胞体外侵袭能力的变化。结果 :转染了正义TFcDNA的HT 2 9细胞的组织因子表达水平及侵袭能力较未转染的HT 2 9细胞升高 ;转染了反义TFcDNA的HT 2 9细胞的组织因子表达水平及侵袭能力较未转染的HT 2 9细胞相比降低。结论 :组织因子可以增加人类大肠癌细胞的体外侵袭能力

 
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