According to the antibody of 65kD chemotropic factor obtained previously,screening library was proceeded using it with the method of ABC,some clones were obtained. The length of one insert was 1 1kb.
5 Out of approximately 120000 plaques, ten positive cDNA clones were isolated from a Fe-deficiency root cDNA expression library of Malus xiaojinensis by screening library for three times using TaMRE-BP cDNA as a probe, then two representative clones were 5' sequenced.
Gene library of strain A12was constructed and an alpha amylase gene amyA1was cloned though screening library of strain A12.Gene amyA1was sequenced and expressed in Escherichia coli JM109(DE3).
The study was undertaken to isolate Fe (II)- transporter cDNA and related binding protein cDNA under Fe-deficiency stress from a Fe-deficiency root cDNA expression library of Malus xiaojinensis by screening library using maize Fe (II)- transporter cDNA and wheat TaMRE-BP cDNA as a probe.
本试验以苹果属小金海棠(Malus xiaojinensis Cheng et Jiang)为试材，利用玉米Fe(Ⅱ)转运蛋白基因片段以及小麦金属反应元件结合蛋白TaMRE-BP cDNA为探针，筛选小金海棠缺铁根cDNA表达文库，目的在于克隆苹果铁高效基因型小金海棠的Fe(Ⅱ)转运蛋白基因以及与缺铁胁迫相关的结合蛋白基因。 主要结果如下：
The main results as follows:1 Out of approximately 120000 plaques, four positive cDNA clones encoding Fe (II)- transporter proteins, designated pFTl -. pFT2, pFT3 and pFT4 respectively, were isolated from a Fe-deficiency root cDNA expression library of Malus xiaojinensis by screening library for three times using Fe (II)-transporter of maize as a probe, then two representative clones (pTFl and pTF2) were sequenced.
Structure-based virtual screening today is basically organized as a sequential process where the molecules of a screening library are evaluated for instance with respect to their fit with a biological target.
In the second scenario, ADME filters are enforced on the entire virtual screening library, prior to performing the docking step.
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If this observation holds true, it further supports the notion that it is a poor strategy to exclude similar compounds from a screening library.
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Evaluation and selection of chemical compounds for a global screening library.
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In order to obtain the cDNA clone of neurotrophic and chemotropic factors produced in injured peripheral nerve tissues, rat sciatic nerves,that have been injured for 15 days in vivo were homogenized followed by purification of mRNA.Then synthesized cDNA was linked to λ gt11 vector and packaged in vitro.The inserting length of cDNA was 0 5 0 8kb.The capacity of cDNA library was 1 5×10 6 with 96 7% of recombinant rate.The titer of library was about 1 4×10 12 pfu/ml.The cDNA of NGF and CNTF was a...
Through screening library with either immobilized or coated enzymes, we selected diseaserelated myristoyltransferase inhibitor phages from phage display random peptide library. After highaffinity bound phages were obtained, they were subjected to in vitro NMT inhibition assay to identify inhibitor phages. The results of DNA sequencing, peptide sequence deducing and sequence aligning of the 16 inhibitor phages suggested that the sequences of inhibitor peptides obtained by the two separa...
Genomic total DNA of swine was digested with enzyme AluⅠand HaeⅢ,which recognition site were 4 bases. The fragments, ranging from 250～600 bp, were ligated to pBluescript KS +, and a fragment-fractionated swine genomic DNA library was constructed. By using oligonucleotides (TG) 10 probe to screening library, 14 positive clones were identified. Two of 14 positive clones were sequenced, and both contain (TG) n microsatellites.
screening library
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