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cotton blue
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  棉蓝
     1. Four stain methods, zinc-chlor-iodide, cotton blue lactophenol, cotton blue and MTT, were evaluated in its efficiency on detection of pathogen mycelia and oospora specifically.
     1.采用I_2-ZnCl_2染色、苯胺蓝染色、棉蓝乳酚油染色、MTT染色等四种染色法检测不同时期玉米病组织及病田中病株种子、正常株种子中的带菌状况、带菌部位、病菌的存活能力。
短句来源
     Sixteen wheat varieties including high,resistant,semi-resistant andsusceptible were obtained from our disease nursery to examine the haus-toria of powdery mildew in relation to resistance by the rapid stainingmethod(Wolf and Fri(?) ,1981)and lactophenol cotton blue solution aswell.
     利用特种蛋白质染料考马斯亮蓝(Coomassie Brilliant Blue R—250)和棉蓝乳酚液快速撕表皮染色法,镜捡16个4种不同抗病类型小麦品种,观察不同抗病性品种与白粉病菌吸器的关系。
短句来源
     Some problems were settled in making permanent slide for predacious nematode fungi by slide cultivation and cotton blue stain.
     采用玻片培养———棉蓝染色法解决了食线虫菌物永久玻片制作中存在的一些问题 ;
短句来源
  棉花蓝
     Researches on Cotton Blue Disease in Vietnam
     越南棉花蓝病害研究进展(英文)
短句来源
  棉兰
     Besides the morphological differentiation of the two organism, rapid detection may be used by treating the conidia with 80% hydrochloric acid and staining with alcohol solutions of 1% brilliant green, 0.1% cotton blue or 0.2% safranine T.
     两菌除从形态学上进行区分外,快速鉴定法可用80%盐酸处理两菌的分生孢子,再用1%亮绿,或0.1%棉兰,或0.2%碱性藏红3种染料的酒精溶液处理。
短句来源
  相似匹配句对
     BLUE
     蓝色
短句来源
     Blue
     蓝蓝
短句来源
     Researches on Cotton Blue Disease in Vietnam
     越南棉花蓝病害研究进展(英文)
短句来源
     Blue Cotton Print in Tianmen, Hubei Province
     湖北天门的蓝印花布
短句来源
     And with the cotton roots,the G.
     G.
短句来源
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  cotton blue
Based on our results we propose that cotton blue disease is associated with a virus with the putative name Cotton leafroll dwarf virus (CLRDV).
      
The observed symptoms and mode of transmission have prompted researchers to speculate that cotton blue disease could be attributed to a member of the family Luteoviridae, but there was no molecular evidence supporting this hypothesis.
      
Cotton blue disease is an aphid-transmitted cotton disease described in Brazil in 1962 as Vein Mosaic "var.
      
Molecular characterization of a virus from the family Luteoviridae associated with cotton blue disease
      
Pollen viability has been investigated in 20 blackberry species using 3 methods, (1) cotton blue, (2) TTC, and (3) germination in a sucrose solution.
      
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Seedlings of Sorghum was prepared by cutting off the roots andstripping off the outer coleoptile at the 3-4 leaf stage.The materialwas placed directly into boiling lactophenol containing 0.1% trypan-blue(or cotton blue)about 20 minutes,until it became soft.The treatedtissue was mounted in rows on a glass slide and covered with anotherslide with pressure so that it could be readily observed undermicroscope. The results showed that the tissue was stained blue, thehead smut mycelium if present was stained...

Seedlings of Sorghum was prepared by cutting off the roots andstripping off the outer coleoptile at the 3-4 leaf stage.The materialwas placed directly into boiling lactophenol containing 0.1% trypan-blue(or cotton blue)about 20 minutes,until it became soft.The treatedtissue was mounted in rows on a glass slide and covered with anotherslide with pressure so that it could be readily observed undermicroscope. The results showed that the tissue was stained blue, thehead smut mycelium if present was stained deep blue and themycelium usually formed short sectional knot-shaped branches.Numerous hyphae were in the base of the meristem of the growingtip, if the seedlings were infected.The percentage of the seedlinginfection was coincidental with that of mature plants infected.The-refore it was considered that this method was useful for the screeningof fungicides for controlling this disease and also for forcasting thedevelopment of the disease in fields. In the present paper the courseof infection of the fungus was traced.In the time before the differen-tiation of the growing top, the hyphae were limited in the meristem.After the differentiation of the floral bud, the hyphae migrated fromthe meristem into the panicle,The period of infection started in thetime of seed germination and ended at the seedlings about 1.5 cm long.The infection was mainly through the coleoptile and the smut hyphaewere present in almost all tissues.

取高粱幼苗切去根部,削去外部叶鞘,露出生长锥,放在乳酚油锥蓝染剂(或棉蓝里)煮染20分钟左右,至材料软化为止,取出夹在两片载玻片里压开,在低倍显微镜下观察,病株生长锥和心叶细胞组织染成浅蓝色,丝黑穗菌菌丝染成深蓝色,呈一段段瘤节状分枝,易于识别。据两年田间对比试验结果,本法预测田间发病率比较准确、简易、快速,适用于室内药剂筛选和田间预测药剂效果。田间高粱出苗后,分期取病株以徒手切片染色观察,侵染菌丝先集中于生长锥基部,后移向生长点里,花芽分化后移进花穗里。成株的高粱病株的每节组织里,均有丝黑穗菌丝存在。丝黑穗菌从高粱幼芽芽鞘侵入,侵入时期从种子发茅到芽长1.5厘米。在高粱幼苗生长锥里的散黑穗菌和坚黑穗菌的菌丝与丝黑穗菌菌丝,在形态上不易区别。

The fungous pathogens of rice blast disease and crab grass blast disease are close members in the same genus, Pyricularia. P. oryzae Cavara is usualy parasitic on rice and P. grisea (Cke. ) Sace. on other cereals and grasses. Besides the morphological differentiation of the two organism, rapid detection may be used by treating the conidia with 80% hydrochloric acid and staining with alcohol solutions of 1% brilliant green, 0.1% cotton blue or 0.2% safranine T. After staining, there will be plasmolysis...

The fungous pathogens of rice blast disease and crab grass blast disease are close members in the same genus, Pyricularia. P. oryzae Cavara is usualy parasitic on rice and P. grisea (Cke. ) Sace. on other cereals and grasses. Besides the morphological differentiation of the two organism, rapid detection may be used by treating the conidia with 80% hydrochloric acid and staining with alcohol solutions of 1% brilliant green, 0.1% cotton blue or 0.2% safranine T. After staining, there will be plasmolysis of conidial cells in P. oryzae but no such reaction in P. grisea. P. grisea may came panicle blast of rice, but it can not infect rice leaves.

稻瘟病菌(Pyricularia oryzae Cavara)与杂草马唐瘟菌(P.grisea [(Cke.)Sacc.]均包含在Pyricularia属内。两菌除从形态学上进行区分外,快速鉴定法可用80%盐酸处理两菌的分生孢子,再用1%亮绿,或0.1%棉兰,或0.2%碱性藏红3种染料的酒精溶液处理。在稻瘟病菌分生孢子的细胞中,明显地出现原生质向细胞中央浓缩的质壁分离现象,而马唐瘟菌则无明显反应。马唐瘟菌可以引致水稻穗颈瘟,却很少侵染叶片产生叶瘟。

Sixteen wheat varieties including high,resistant,semi-resistant andsusceptible were obtained from our disease nursery to examine the haus-toria of powdery mildew in relation to resistance by the rapid stainingmethod(Wolf and Fri(?),1981)and lactophenol cotton blue solution aswell.The results clearly pointed out that resistance in wheat to be infec-ted by mildew could be conferred as follows:(1)delay the early haus-storia development,(2)increase of haustorial rate rather slow,(3)reduc-tion the numbers and...

Sixteen wheat varieties including high,resistant,semi-resistant andsusceptible were obtained from our disease nursery to examine the haus-toria of powdery mildew in relation to resistance by the rapid stainingmethod(Wolf and Fri(?),1981)and lactophenol cotton blue solution aswell.The results clearly pointed out that resistance in wheat to be infec-ted by mildew could be conferred as follows:(1)delay the early haus-storia development,(2)increase of haustorial rate rather slow,(3)reduc-tion the numbers and final size of haustoria,and(4)distortion and di-sintegration of haustria more quickly.It was also shown that more haustriahappened in the seelingsand juvenile plants than in the old plants.The present experimental results probably could be used to detectsome resistant materials early for breeding program.

利用特种蛋白质染料考马斯亮蓝(Coomassie Brilliant Blue R—250)和棉蓝乳酚液快速撕表皮染色法,镜捡16个4种不同抗病类型小麦品种,观察不同抗病性品种与白粉病菌吸器的关系。得出抗病品种有下列特点:①抗病类型延迟吸器的形成;②吸器数目增加缓慢;③吸器数量少而小;④吸器消衰快。小麦苗期的吸器数量比成株多。利用快速染色法可以提供早期选择抗病和淘汰感病材料之用。

 
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