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chain reation
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  链反应
     Methods: DR4,DR5,DcR1 and DcR2 mRNA expression and its level were assessed in 23 cases of normal liver tissue,40 cases of para-liver cancer tissuse and 40 cases of hepatocellular carcinoma tissue with semi-quantitative reverse transcription polymerase chain reation (RT-PCR) procedure.
     方法:应用半定量逆转录多聚酶链反应(RT-PCR)方法检测40例人肝细胞癌组织、相应癌旁肝组织、23例正常人肝组织中DR4、DR5、DcR1、DcR2的mRNA表达率及表达水平。
短句来源
     HCMV-DNA in human whey was determined by polymerase chain reation (PCR).
     多聚酶链反应(PCR)技术检测乳清HCMV-DNA。
短句来源
  “chain reation”译为未确定词的双语例句
     Methods Made rat actue hepatice failure by injecting of D-galactosamine,the expressions of toll like receptor 4 and CD14 mRNA were detected by reverse transcriptase polymerase chain reation at 6,12,24,48,72,120,148 hours point.
     方法用D-氨基半乳糖剂量造成大鼠急性肝衰竭模型,用RT-PCR方法检测toll样受体,CD14 mRNA在D-氨基半乳糖介导的大鼠急性肝衰竭6、12、24、48、72、120和148 h的表达。
短句来源
     Methods: Expressions of PTTG and c-myc gene mRNA were detected in bone marrow mononuclear cell (BMMNC) from 34 patients with CML(16 chronic phase, 11 accelerated phase and 7 blast crisis) and 10 controls by reverse transcriptase-polymerase chain reation (RT-PCR).
     方法:采用RT-PCR方法检测34例CML患者(慢性期16例,加速期11例,急变期7例)及10例对照者骨髓单个核细胞(BMMNC)中PTTG和c-myc基因的表达。
短句来源
     Methods: Reverse transcription- polymerase chain reation (RT-PCR) technique, was used to clone hLMP-1 and hBMP-2 from human placenta tissue and plasmid with hBMP-2. Digestion snd ligations with eukaryotic expression plasmid pIRES2-EGFP, pIRES2-EGFP-LMP1 and pIRES2-EGFP-BMP2 recombinants were constructed.
     方法:采用RT-PCR的方法分别从人的胎盘组织和含有人BMP-2的重组质粒中,克隆LMP-1和BMP-2,与真核表达质粒pIRES2-EGFP酶切连接,构建pIRES2-EGFP-LMP1及pIRES2-EGFP-BMP2重组质粒。
短句来源
     Detection of bcr/abl Fusion Gene in 22 Chronic Granulocytic Leukemia Patients by Reverse Transcriptase Polymerse Chain Reation
     筑巢式PCR检测22例慢性粒细胞白血病患者bcr/abl融合基因结果分析
短句来源
     Methods:The reverse transcription polymerase chain reation(RT-PCR) and Western blot were employed to measure the expression of LPA-1,LPA-2,and LPA-3 receptor mRNA and LPA-2 and LPA-3 receptor protein expression in human epithelial ovarian neoplasms.
     方法:采用半定量RT-PCR和Western blot检测LPA1、LPA2和LPA3mRNA以及LPA2和LPA3蛋白在人卵巢上皮性肿瘤组织中的表达水平。
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  相似匹配句对
     Chain of Love
     爱的涟漪
短句来源
     The Chain Method
     关于连锁法
短句来源
     The Study on Sex Determination of Bovine Embryos by Polymerase Chain Reation (PCR)
     牛早期胚胎性别鉴定的PCR方法的研究
短句来源
     HCMV-DNA in human whey was determined by polymerase chain reation (PCR).
     多聚酶链反应(PCR)技术检测乳清HCMV-DNA。
短句来源
     reation time, 2h;
     反应时间2h;
短句来源
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A total number of 110 cases of early pregnant women' s urine specinens and their embryos'chorionic Villous samples(CVS)were screened for HCMV DNA by polymerase chain reation .Therates of positivity for the mother's urine specimens and for their embryos's CVs were 15.5%(17/110)and 7.3%(8/110)aspectively.Thus the rate of positive HCMV intrauterine infectionamong the positive mothers was 41.2%. Statistic analysis showed that among early pregnantwomen the intiauterine infforon was closely correlated with the...

A total number of 110 cases of early pregnant women' s urine specinens and their embryos'chorionic Villous samples(CVS)were screened for HCMV DNA by polymerase chain reation .Therates of positivity for the mother's urine specimens and for their embryos's CVs were 15.5%(17/110)and 7.3%(8/110)aspectively.Thus the rate of positive HCMV intrauterine infectionamong the positive mothers was 41.2%. Statistic analysis showed that among early pregnantwomen the intiauterine infforon was closely correlated with the positive urine specirnens for HCMV(P<0.01).This suggested that excreling HCMV in the urine nught be an indication of genelalactlve infection of the mother.The difference of HCMV positve embcyos'CVS between whosemothers urihe were also positive and those their urine were negetive was statistically significant(P<0.01).These results revealed that the embryo is at high risk of HCMV intrauterineinfection when his mother has had a general active infection.It was suggested that theurine specimens of all pregnant women should be screened,HCMV by PCR if possible.

用聚合酶链反应(PCR)技术检测110例早孕妇女尿液及其胚胎绒毛的HCMVDNA。结果孕母尿液与胚胎绒毛的HCMvDNA阳性率分别为15.5%和7.3%,宫内感染率为4l2%,孕母尿液与胚胎绒毛的HCMV阳性数密切相关(P<0.01),提示孕母尿路排毒系全身性活动性感染;孕母尿液HCMV阳性与阴性者其胚胎绒毛HCMV阳性数具显著差异(P<0.01),表明孕母尿液阳性者较阴性者处于垂直感染胚胎的高危状态,建议采用PCR法普查早孕妇女尿液HCMVDNA,并对阳性者严密监视胎儿发育情况,酌情采取相应措施。

Polymerase Chain Reation(PCR)was induced to indentify human papillomaviruses (HPVs) from 70 paraffin-embedded specimens of condyloma accuminatum (CA)and papillomas.The results showed that the positive rate of HPV-DNA was 70.0%(49/70),The Mulfi-infection rate was 53.1%in all the positive specimens.The major epidemic type of HPV in condyloma accuminatum and papillomas were HPV6 and/or HPV11 in Shantou district.

本文应用聚合酶链反应检测了70例尖锐湿疣、乳头瘤石蜡包理组织中的人乳头瘤病毒DNA,结果表明:HPV-DNA的检出率为70.0%(49/70),多重感染占阳性例数的53.1%。HPV6和(或)HPV11为本地区尖锐湿疣、乳头瘤组织中HPV感染的主要型别。

HCV NS5b cDNA from two HCV infected individuals were sequenced with polymerase-chain-reation (PCR)-directed sequencing method. Compared with HCV-J, 91. 84% and 92. 36 % nucleotides homology, 91. 84% and 95. 79% homology with HC-C2,were found in HC114 and HC-C11 respectively. A three nucleotide deletion occurred in HC-114. As the generated profiles of sequencing ladders were clear, it indicated that PCR-directed sequencing method worked best in showing larger range variations as well as individual...

HCV NS5b cDNA from two HCV infected individuals were sequenced with polymerase-chain-reation (PCR)-directed sequencing method. Compared with HCV-J, 91. 84% and 92. 36 % nucleotides homology, 91. 84% and 95. 79% homology with HC-C2,were found in HC114 and HC-C11 respectively. A three nucleotide deletion occurred in HC-114. As the generated profiles of sequencing ladders were clear, it indicated that PCR-directed sequencing method worked best in showing larger range variations as well as individual base variation.

用PCR分析HCV-RNANS5部分序列变异魏来,王宇,陈红松,陶其敏(北京医科大学人民医院肝病研究所,北京100044)对丙型肝炎病毒(HCV)cDNA的序列分析是验证HCV基因分型,确定新的型与亚型的基础 ̄[1].经典的序列分析方法需经繁琐的分子...

 
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