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matrix gene
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  基质基因
     SELECTIVE DOWN-REGULATION OF EXTRACELLULAR MATRIX GENE EXPRESSION BY BONE MARROW DERIVED STEM CELLTRANSPLANTATION INTO INFARCTED MYOCARDIUM IN RATS
     骨髓基质干细胞移植选择下调大鼠心肌梗死细胞外基质基因表达
短句来源
     Conclusion Anterior disc displacement leads to alteration of extracellurar matrix gene expression in the condylar chondrocyte, which means the start of remodeling.
     结论 关节盘前移位后髁突软骨的基质基因表达发生有序、协调的变化 ,这种变化意味着适应性改建的启动。
短句来源
  “matrix gene”译为未确定词的双语例句
     The Cartilage Matrix Gene Expression in Rabbit TMJ Posterior Attachment Following Disc Displacement
     兔颞下颌关节盘前移位后后附着内软骨基质基因的表达
     A pair of primers and a probe were designed according to the sequences of the Matrix gene of North American type PPRSV, and a real-time RT-PCR assay was established to detect this virus.
     参照美洲型PRRSV M基因序列,设计引物和探针,建立荧光RT-PCR检测方法,用于该型病毒的检测。
短句来源
     These samples cDNAs contained multiple fragments of AIV including the hemagglutinin gene and matrix gene.
     样品 cDNAs是一个包括禽流感病毒HA和M基因的混合物。
短句来源
     The type of AIV were detected with the matrix gene,and the subtypes of AIV were differentiated with hemagglutinin gene.
     依据M基因鉴别型,依据HA基因鉴别亚型。
短句来源
     A real-time RT-PCR assay for the detection of North American type porcine reproductive and respiratory syndrome virus (PPRSV) was developed using a pair of primers derived from the published sequences of the Matrix gene of PPRSV. This assay could detect 1 TCID50 of the virus with a high specificity.
     参照美洲型猪繁殖与呼吸综合征病毒(PRRSV)M基因序列,设计引物和探针,并建立其荧光RT-PCR检测方法,以检测严重危害我国养猪业的此型PRRSV。 该法能检测到1 TCID50的PRRSV。
短句来源
  相似匹配句对
     Expression of matrix metalloproteinase-14 gene in endometriosis
     子宫内膜异位症中基质金属蛋白酶-14基因的表达
短句来源
     Study on matrix metalloproteinase-3 protein and its gene in atherosclerosis
     基质金属蛋白酶-3蛋白及基因在动脉硬化血管中表达意义的研究
短句来源
     Matrix Polymerization
     聚合新技术—模板(MatriX)聚合(Ⅰ)
短句来源
     Normalization of Matrix
     矩阵的正规化
短句来源
     Rescue Gene
     抢救植物基因
短句来源
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  matrix gene
The nucleoprotein and matrix gene had highest nucleotide sequence similarity to the H6 subtypes A/Duck/Hong Kong/3096/99 (H6N2) and A/WDk/ST/1737/2000 (H6N8), respectively.
      
Of the several primer pairs tested, two sets of primers derived from the matrix gene of APV were able to specifically detect the viral RNA of APV.
      
Multiple pairs of specific primers were designed to amplify a portion of the phosphoprotein gene, the matrix gene, and the glycoprotein gene of SVCV genogroup Id (corresponding to nucleotides 2174-4942 of GenBank accession NC_002803).
      
Genomic Analysis of Matrix Gene and Antigenic Studies of its Gene Product (M1) of a Swine Influenza Virus (H1N1) Causing Chronic
      
The production of versican by monocytes in the infarct area represents a novel finding of the expression of an extracellular matrix gene by monocytes in the infarcted heart.
      
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Objective To establish a rapid, specific and effective technique for differentiating types A and B of influenza viruses in clinical isolates. Methods Two primer sets were designed according to the nucleotide sequences of Matrix genes of influenza A and B. First strand of viral cDNA was synthesized from viral RNA by using Oliogo d (T) 18 . PCR was performed with a mixture of the two primer sets specific for influenza types A and B, respectively. Amplified products were visualized in 1.2 % agarose...

Objective To establish a rapid, specific and effective technique for differentiating types A and B of influenza viruses in clinical isolates. Methods Two primer sets were designed according to the nucleotide sequences of Matrix genes of influenza A and B. First strand of viral cDNA was synthesized from viral RNA by using Oliogo d (T) 18 . PCR was performed with a mixture of the two primer sets specific for influenza types A and B, respectively. Amplified products were visualized in 1.2 % agarose gel containing ethidium bromide. Types A and B were differentiated by the sizes of the products (506bp for type A and 240bp for type B). Results Twenty nine isolates from clinical specimens which have been typed by hemagglutination inhibition (HAI) were identified by the method described above and the results of identified types of influenza viruses were consistent with those obtained with the HAI test and sequencing of the HA genes. Conclusion The RT PCR technique described here is simple, convenient, specific and therefore can be used for detection and identification of influenza viruses A and B in clinical isolates. It can provide a useful alternative to existing methods of influenza identification and offer the basis for clinical diagnosis, prevention and timely specific treatment.

目的 建立快速、特异、有效的鉴别甲、乙型流感病毒的方法。方法 根据编码甲、乙型流感病毒膜蛋白M基因的核苷酸序列设计两对引物 ,将这两对引物用于同一逆转录 聚合酶链反应(RT PCR) ,甲、乙型毒株的扩增产物分别为 5 0 6bp和 2 40bp ,根据这些产物在 1.2 %的琼脂糖凝胶电泳上的大小 ,即可鉴别所测毒株的型别。结果 用这一方法对我国 2 4株甲型和 5株乙型流感病毒分离株进行型别鉴定 ,分型结果与血凝抑制试验和核苷酸测定结果完全相符。结论 用上述根据M基因序列设计的RT PCR方法鉴别甲、乙型流感病毒具有快速、简便、特异等特点 ,适用于流感病毒型别鉴定 ,可为临床诊断和及时制定防治措施提供依据

Objective To investigate lung carcinogenesis associated genes in human lung squamous cell carcinoma and malignant transformation of human bronchial epithelial cells induced by chemical carcinogens with cDNA microarray. Methods The gene expression patterns were detected in all specimens by cDNA microarray which representing 4 096 different human genes. The differences in gene expression among 6 cases of human lung squamous cell carcinoma tissues and 6 normal lung tissues were analyzed....

Objective To investigate lung carcinogenesis associated genes in human lung squamous cell carcinoma and malignant transformation of human bronchial epithelial cells induced by chemical carcinogens with cDNA microarray. Methods The gene expression patterns were detected in all specimens by cDNA microarray which representing 4 096 different human genes. The differences in gene expression among 6 cases of human lung squamous cell carcinoma tissues and 6 normal lung tissues were analyzed. The different gene expression patterns between the normal human bronchial epithelial cell lines (16HBE) and the malignant transformation of human bronchial epithelial cells induced by Benzo(a)pyrene metabolite BPDE (anti-Benzo(a)pyrene diol-epoxide,BPDE) and crystalline nickel sulfide were also studied by that method. The similar changed genes among those gene expression patterns were identified as lung carcinogenesis associated genes. Results Among the 4096 genes of cDNA microarrays, there were 171 genes expressed differently among lung cancer tissues and normal lungs, 143 genes expressed differently between BPDE transformed cells and normal 16HBE cell lines, 151 genes differed between nickel sulfide transformed cells and normal 16HBE cell lines. By comparing the gene expression profiles, there were 89 similar changed genes which might be associated with human lung carcinogenesis, 39 of which were up regulated: 6 oncogenes, 4 cell cycle control genes, 6 cell proliferation genes, 8 metastasis genes, 3 neuroendocrine genes, 1 drug-resister gene, 1 anti-apoptosis gene, 1 oxidative gene and other 9 genes. 50 genes were down-regulated: 7 tumor suppression genes, 11 DNA repair genes, 1 antioxidant genes, 3 GST family genes, 3 cell framework genes, 2 apoptosis induced genes, 5 signal conduction genes, 5 cytokines and their receptor genes, 7 metabolization genes, 1 cell matrix genes, and other 5 genes. Conclusion cDNA microarray can be applied to study gene expression profiles effectively and to screen human lung carcinogenesis associated genes.

目的 使用基因芯片技术研究肺鳞癌及化学致癌物诱导人气管上皮细胞恶性转化的癌变相关基因。方法 应用含 4 0 96条人类全长基因的cDNA芯片分别检测 6例肺鳞癌和 6例正常肺组织的基因表达谱 ;并用上述芯片研究苯并 (a)芘代谢产物BPDE[anti Benzo(a) pyrenediol epoxide,BPDE]和结晶型硫化镍所诱导的人支气管上皮细胞恶性转化与正常的人支气管上皮细胞系 (16HBE)在基因表达谱上的差异 ;将 3类标本共同的差异表达基因确定为肺癌变的相关基因。结果 在肺鳞癌 /正常肺组织之间、BPDE诱导的恶性转化细胞 /正常 16HBE细胞之间及硫化镍诱导的恶性转化细胞 /正常 16HBE细胞之间发现差异表达基因分别为 171条、14 3条和 15 1条。通过比较 ,发现 89条与肺癌变相关的共同基因 ,其中表达显著增加的基因 39条 ,它们是 :癌基因 6条 ;细胞周期相关基因 4条 ;细胞增殖基因 6条 ;肿瘤转移基因 8条 ;神经内分泌基因 3条 ;耐药基因 1条 ;凋亡抑制基因 1条 ;氧化基因 1条 ;其他基因 9条。表达显著下降的基因 5 0条 ,它们是 :抑癌基因 7条 ;...

目的 使用基因芯片技术研究肺鳞癌及化学致癌物诱导人气管上皮细胞恶性转化的癌变相关基因。方法 应用含 4 0 96条人类全长基因的cDNA芯片分别检测 6例肺鳞癌和 6例正常肺组织的基因表达谱 ;并用上述芯片研究苯并 (a)芘代谢产物BPDE[anti Benzo(a) pyrenediol epoxide,BPDE]和结晶型硫化镍所诱导的人支气管上皮细胞恶性转化与正常的人支气管上皮细胞系 (16HBE)在基因表达谱上的差异 ;将 3类标本共同的差异表达基因确定为肺癌变的相关基因。结果 在肺鳞癌 /正常肺组织之间、BPDE诱导的恶性转化细胞 /正常 16HBE细胞之间及硫化镍诱导的恶性转化细胞 /正常 16HBE细胞之间发现差异表达基因分别为 171条、14 3条和 15 1条。通过比较 ,发现 89条与肺癌变相关的共同基因 ,其中表达显著增加的基因 39条 ,它们是 :癌基因 6条 ;细胞周期相关基因 4条 ;细胞增殖基因 6条 ;肿瘤转移基因 8条 ;神经内分泌基因 3条 ;耐药基因 1条 ;凋亡抑制基因 1条 ;氧化基因 1条 ;其他基因 9条。表达显著下降的基因 5 0条 ,它们是 :抑癌基因 7条 ;DNA修复基因 11条 ;抗氧化基因 1条 ;GST基因家族 3条 ;细胞骨架基因 3条 ;凋亡诱导基因 2条 ;信号传导基因 5条 ;细胞因子及其受体基因 5条 ;细胞代谢基因 7条 ,细胞外基质基因 1条 ;?

The invasion and metastasis of malignant tumor cells are important factors causing the death of cancer patients. The proteolytic activity of proteinases to most of the extracellular matrix macromolecules is closely correlated with the invasion and metastasis of malignant tumor cells . Matrix metalloproteinases(MMP) are key proteinases involved in these processes. MMP is a type of Zn 2+ depended proteinases. MMP2 and MMP9 are the unique types of proteinase that hydrolyze the bone structure of excellulary...

The invasion and metastasis of malignant tumor cells are important factors causing the death of cancer patients. The proteolytic activity of proteinases to most of the extracellular matrix macromolecules is closely correlated with the invasion and metastasis of malignant tumor cells . Matrix metalloproteinases(MMP) are key proteinases involved in these processes. MMP is a type of Zn 2+ depended proteinases. MMP2 and MMP9 are the unique types of proteinase that hydrolyze the bone structure of excellulary matrix (type IV collagen). So they are particularly correlated with leukemia cells infiltration and metastasis. This review aims to introduce the function of MMP and the regulation of matrix gene expression, as well as their roles in leukemia cell invasion and metastasis. A new strategy that MMP may be a therapeutic target in the treatment of leukemia is particularly introduced.

恶性肿瘤细胞的侵袭和转移是造成肿瘤病人死亡的重要原因 ,肿瘤细胞发生侵袭和转移与蛋白水解酶水解胞外基质密切相关 ,锌离子依赖性的基质金属蛋白酶 (matrixmetalloproteinases,MMP)中的MMP2和MMP9是参与此过程的两个关键性蛋白酶。本文就MMP结构、生理调控、在白血病发生和转移中的作用及针对此靶位点开展白血病治疗等方面的研究进展进行综述

 
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