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protein crosslink     
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  蛋白质交联
     Conclusion \ High dose arsenic has damage effects on marrow cell's DNA of mice in vivo and induce DNA\|protein crosslink at same time. Lower dose arsenic can affect the sensitivity of DNA to other carcinogen.
     【结论】 高剂量的砷在小鼠体内对骨髓细胞DNA有损伤作用 ,并可诱发DNA 蛋白质交联 ,而低剂量的砷可能影响DNA对其他诱变剂的敏感性
短句来源
     Objective To clarify the value of DNA Protein crosslink(DPC) and its detection assay in dermal lesion monitoring of arsenism patients due to coal burning.
     目的 探讨 DNA-蛋白质交联 (DPC)在监测砷中毒遗传损伤及皮肤病变的发生发展中的应用价值。
短句来源
     Methods Subjects were divided into 5 groups with pathological examination. And DNA Protein crosslink was detected with 125 I postlabelling assay.
     方法 依据皮肤病理组织学检查结果对砷接触者进行分组 ,并采用 1 2 5I-后标记法测定其 DNA-蛋白质交联状况。
短句来源
     Conclusions As a kind of sensitive molecular biomarker,simple,and rapid detection method 125 I postlabelling assay, DNA Protein crosslink can be used in spot dynamic monitor in arsenism area to find people at high risk. So it has practical value in prevention of arsenic induced dermal carcinoma.
     结论  DNA-蛋白质交联是一敏感的分子遗传损伤标记 ,其检测方法 1 2 5I-后标记法简便、快速 ,在砷中毒的现场监测中可以早期发现癌变高危对象及采取针对性处理 ,对防止砷性皮肤癌的发生有实际应用价值
短句来源
  蛋白质交联
     Conclusion \ High dose arsenic has damage effects on marrow cell's DNA of mice in vivo and induce DNA\|protein crosslink at same time. Lower dose arsenic can affect the sensitivity of DNA to other carcinogen.
     【结论】 高剂量的砷在小鼠体内对骨髓细胞DNA有损伤作用 ,并可诱发DNA 蛋白质交联 ,而低剂量的砷可能影响DNA对其他诱变剂的敏感性
短句来源
     Objective To clarify the value of DNA Protein crosslink(DPC) and its detection assay in dermal lesion monitoring of arsenism patients due to coal burning.
     目的 探讨 DNA-蛋白质交联 (DPC)在监测砷中毒遗传损伤及皮肤病变的发生发展中的应用价值。
短句来源
     Methods Subjects were divided into 5 groups with pathological examination. And DNA Protein crosslink was detected with 125 I postlabelling assay.
     方法 依据皮肤病理组织学检查结果对砷接触者进行分组 ,并采用 1 2 5I-后标记法测定其 DNA-蛋白质交联状况。
短句来源
     Conclusions As a kind of sensitive molecular biomarker,simple,and rapid detection method 125 I postlabelling assay, DNA Protein crosslink can be used in spot dynamic monitor in arsenism area to find people at high risk. So it has practical value in prevention of arsenic induced dermal carcinoma.
     结论  DNA-蛋白质交联是一敏感的分子遗传损伤标记 ,其检测方法 1 2 5I-后标记法简便、快速 ,在砷中毒的现场监测中可以早期发现癌变高危对象及采取针对性处理 ,对防止砷性皮肤癌的发生有实际应用价值
短句来源
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  protein crosslink
Pentosidine is a pentose-derived protein crosslink, which forms from glycated proteins in the presence of oxygen.
      
From protein-protein crosslink data and the cryo-EM map of the S.cerevisiae small ribosomal subunit, we suggest that Rps19p is localized in close vicinity to the Nep1p 18S rRNA binding site.
      
Further studies in this population are needed to confirm the possible association between the high levels of DNA-Protein crosslink and Cr exposure.
      
The mean DNA-Protein crosslink level in the lymphocytes of the exposed group was 1.3±0.5% (SD), whereas the unexposed group had 0.8±0.4% (p>amp;lt;0.001), after adjustment for age, gender, race, smoking, and weight.
      
Since one of the major lesions induced in intact cells by chromate is the DNA-Protein crosslink, we have used this lesion as a biomarker of biological effect of chromium (Cr) exposure.
      


On the basis of Kohn's alkaline elution technique,a method which could detect DNA interstrand crosslink and DNA-protein crosslink simutaneously had been established.The parameter of Crosslink Degree suggested in this paper could quantitate the two kinds of DNA crosslinks.The existence of DNA-protein crosslinks was verified.

本方法以DNA单链断裂的检测为基础,在背景γ射线照射下进行DNA交联检测。所建方法与Kohn氏原法相比,洗脱时间大为缩短,实验所用主要材料都能立足国内。本文引入“交联度”这个参数,能同时相对定量地表示DNA总交联、DNA-蛋白质交联和DNA链间交联。此外还从DNA、蛋白质两方面确证了DNA-蛋白质交联的存在。

AIM: To study the mechanism of cisplatin interaction with DNA, and the attenuating effects of 6,7-dimethoxycoumarin ( DMOC ) on crosslink. METHODS: Primary cultured rabbit kidney proximal tubular cells (PTC) were established. DNA interstrand crosslink was assayed with ethidium bromide binding and DNA-protein crosslink with 125I-postlabelling. PTC were incubated with cisplatin for 24 h. DMOC was preincubated with PTC for 24 h, and cisplatin (26 μmol·L-1) was added into culture and incubated for another...

AIM: To study the mechanism of cisplatin interaction with DNA, and the attenuating effects of 6,7-dimethoxycoumarin ( DMOC ) on crosslink. METHODS: Primary cultured rabbit kidney proximal tubular cells (PTC) were established. DNA interstrand crosslink was assayed with ethidium bromide binding and DNA-protein crosslink with 125I-postlabelling. PTC were incubated with cisplatin for 24 h. DMOC was preincubated with PTC for 24 h, and cisplatin (26 μmol·L-1) was added into culture and incubated for another 24 h. RESULTS: Cisplatin induced formation of DNA interstrand crosslink (13, 26, 52, and 78 μmol·L-1) and DNA-protein crosslink (26, 52, and 78μmol·L-1) (P<0.01). DNA interstrand crosslink in DMOC (0.4, 4, and 8 mg · L-1) and DNA-protein crosslink in DMOC (4,8 mg·L-1) were less than those in cisplatin group (26 μmol·L-1), respectively ( P < 0.01). CONCLUSION: The mechanisms of cisplatin interaction with DNA in PTC were DNA interstrand crosslink and DNA-protein crosslink, and DMOC attenuated these effects in vitro.

目的:研究顺铂与肾近端小管DNA的作用机制,和茵陈素的干预作用,方法:原代培养兔肾近端小管细胞(PTC).溴乙锭荧光测DNA链间交联,~(125)Ⅰ标记测DNA-蛋白交联,顺铂与PTC保温24h.茵陈素与PTC提前24 h保温后,加入顺铂26μmol·L~(-1)再保温24 h,结果:顺铂13到78 μmol·L~(-1)和26到78 μmol·L~(-1)可使PTC形成DNA链间交联和DNA-蛋白交联,茵陈素(0.4,4,8 mg·L~(-1))和(4,8 mg·L~(-1))组,DNA链间交联和DNA-蛋白交联分别低于顺铂(26μmol·L~(-1)),结论:顺铂导致肾近端小管形成DNA链间交联和DNA-蛋白交联,茵陈素减弱这两种作用。

Objective To study the damage effect and its mechanism of Arsenic on DNA of cells in vivo. Methods \ The DNA break in bone marrow cells from the mice which have drunk the water containing As\-2O\-3 for 6 months were analyzed with Single cell gel electrophoresis. Results \ The length of DNA migration and the rate of DNA migration of the bone marrow cells increased remarkably at high dose(12\^5 mg/L)group( P <0\^05) and there were no significant difference between medium,low dos(2\^5,0\^5 mg/L) group...

Objective To study the damage effect and its mechanism of Arsenic on DNA of cells in vivo. Methods \ The DNA break in bone marrow cells from the mice which have drunk the water containing As\-2O\-3 for 6 months were analyzed with Single cell gel electrophoresis. Results \ The length of DNA migration and the rate of DNA migration of the bone marrow cells increased remarkably at high dose(12\^5 mg/L)group( P <0\^05) and there were no significant difference between medium,low dos(2\^5,0\^5 mg/L) group and control group.Cyclophosphamide 20 mg/kg which do not induce DNA break was intraperitoneally injected to 6 mice each group,the length of DNA migration and the rate of DNA migration of the bone marrow cells were significantly higher than those of both control group which were injected cyclophosphamide only and same As\-2O 3 dose group which were not injected cyclophosphamide.The lysed cells were also treated with proteinase K, the values were higher than those which were not treated with proteinase K( P <0\^05). Conclusion \ High dose arsenic has damage effects on marrow cell's DNA of mice in vivo and induce DNA\|protein crosslink at same time.Lower dose arsenic can affect the sensitivity of DNA to other carcinogen.

 【目的】 了解砷对体内细胞DNA的损伤作用及其机制。【方法】 在小鼠饮水中加入As2 O3,喂养 6个月 ,采用单细胞凝胶电泳技术对骨髓细胞DNA断裂进行了分析。【结果】 高剂量组 ( 12 5mg/L)小鼠骨髓细胞DNA的迁移度和迁移率显著高于阴性对照组 ,P <0 0 5 ;中、低剂量组 ( 2 5、0 5mg/L)小鼠骨髓细胞DNA的迁移度和迁移率与阴性对照组无显著差异。各剂量组另选 6只小鼠 ,以不引起明显DNA断裂剂量 ( 2 0mg/kg)的环磷酰胺进行腹腔注射 ,骨髓细胞DNA的迁移度和迁移率显著高于接受 2 0mg/kg环磷酰胺或As3O3单独作用的小鼠 (P <0 0 5 )。用蛋白酶K处理裂解后的细胞高剂量组的迁移度与迁移率显著高于未处理的细胞 (P <0 0 5 )。【结论】 高剂量的砷在小鼠体内对骨髓细胞DNA有损伤作用 ,并可诱发DNA 蛋白质交联 ,而低剂量的砷可能影响DNA对其他诱变剂的敏感性

 
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