助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   corticosterone binding 的翻译结果: 查询用时:0.208秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

corticosterone binding
相关语句
  皮质酮结合
     The presence of low-affinity [3H]corticosterone binding sites in plasma membrane isolated from rat liver was determined.
     分离大鼠肝细胞质膜级分中存在低亲和力的[~3H]皮质酮结合位点。
短句来源
  “corticosterone binding”译为未确定词的双语例句
     Displacement experiments showed that when RU38486 concentration reached 100~280-fold that of [3H]corticosterone, it began to inhibit [3H]corticosterone binding, while low concentration of RU38486 had no inhibitory effect.
     置换实验显示低浓度的RU38486对[~3H]皮质酮的结合无抑制效应,当浓度达到[~3H]皮质酮的100~280倍时,才开始抑制[~3H]皮质酮的结合。
短句来源
  相似匹配句对
     protein binding;
     蛋白结合;
短句来源
     Plasma corticosterone (B) concentration was determined by competitive protein-binding radioas- say.
     用竞争性蛋白结合分析法测定了血浆中皮质酮(B)的浓度。
短句来源
     Plasma corticosterone(CC) concentration was determined by competitive protein-binding assay.
     同时检测血浆皮质酮含量(CC)。
短句来源
     The binding energy of element C and N in C.
     采用X光电子能谱仪测出了3种形式的C.
短句来源
     Corticosterone is one of the adrenal hormones.
     皮质酮是肾上腺皮质激素之一。
短句来源
查询“corticosterone binding”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  corticosterone binding
Perfusion of rats with saline eliminated most liver and kidney corticosterone binding but did not affect CB in WAT.
      
Corticosterone binding (CB) capacity was determined in visceral and subcutaneous white adipose tissue (WAT), as well as in plasma of lean Zucker rats.
      
Competitive binding studies with analogue steroids indicated that corticosterone binding to kidney plasma membrane is hormone-specific.
      
From autoradiographic studies we suggest these corticosterone binding sites are located in the whole adrenal cortex.
      
Corticosterone binding was found to be time-dependent, saturable and reversible, and was reduced by more than 70% when membranes were pretreated with proteases.
      
更多          


The presence of low-affinity [3H]corticosterone binding sites in plasma membrane isolated from rat liver was determined. The kinetic parameters of this binding estimated by LIGAND program were Kd=4.11±0.57umol/L and Bmax=68.87±4.64pmol/mg protein. The affinities of five steroids for the membrane binding sites in competition with the binding of [3H]corticosterone were of the following or der: progesterones=dexamethasone>RU26988>RU38486>17B~estradiol. Displacement experiments showed...

The presence of low-affinity [3H]corticosterone binding sites in plasma membrane isolated from rat liver was determined. The kinetic parameters of this binding estimated by LIGAND program were Kd=4.11±0.57umol/L and Bmax=68.87±4.64pmol/mg protein. The affinities of five steroids for the membrane binding sites in competition with the binding of [3H]corticosterone were of the following or der: progesterones=dexamethasone>RU26988>RU38486>17B~estradiol. Displacement experiments showed that when RU38486 concentration reached 100~280-fold that of [3H]corticosterone, it began to inhibit [3H]corticosterone binding, while low concentration of RU38486 had no inhibitory effect.

分离大鼠肝细胞质膜级分中存在低亲和力的[~3H]皮质酮结合位点。利用LIGAND程序计算该结合的参数为:Kd=4.11±0.57μmol/L,B_(max)=68.87±4.64pmol/mg蛋白。5种甾体取代皮质酮特异性结合的能力依次为:孕酮≈地塞米松>RU26988>RU38486>17β雌二醇。置换实验显示低浓度的RU38486对[~3H]皮质酮的结合无抑制效应,当浓度达到[~3H]皮质酮的100~280倍时,才开始抑制[~3H]皮质酮的结合。

By the method of radioligand binding assay,the [3H]corticosterone binding to synaptic plasma membranes (SPM), which was isolated from porcine cerebrum cortex, was estimated, to which the binding was specific and saturable. The saturation binding curve was a S-shaped one,and a convex curve could be seen in Scatchard plot of the specific binding data. After mathematical treatment with computer, the maximal binding capacity (Bmax) was calculated to be 331. 8±36. 9 fmol/mg protein,...

By the method of radioligand binding assay,the [3H]corticosterone binding to synaptic plasma membranes (SPM), which was isolated from porcine cerebrum cortex, was estimated, to which the binding was specific and saturable. The saturation binding curve was a S-shaped one,and a convex curve could be seen in Scatchard plot of the specific binding data. After mathematical treatment with computer, the maximal binding capacity (Bmax) was calculated to be 331. 8±36. 9 fmol/mg protein, and the apparent equilibrium dissociation constant (Kd), to be 185. 0±62. 7nmol/L. The competition replacement experiments carried out with one-point binding assay,revealed that the glucocorticoid membrane binding sites(GCMBS) on SPM had relative steroid binding specificity. The relative affinity was corticosterone>progesterone≈17 beta-estradiol≈aldosterone > testosterone.

利用放射配体结合测定法,测得猪大脑皮层突触质膜上存在有皮质酮特异结合位点,该皮质酮膜结合位点的最大结合量(Bmax)为331.8±36.9fmol/mg蛋白,平衡解离常数(Kd)为185.0±62.7nmol/L。竞争取代实验表明,它具有较高的甾体结合特异性,按亲和力大小排列为:皮质酮>孕酮>17β-雌二醇≈醛固酮>睾酮。

To investigate the nongenomic effect of the glucocorticoid corticosterone on nicotinic acetylcholine receptor (nAChR) in PC12 cells. METHODS; The acetylcholine (ACh)-induced current was measured on nerve growth factor differentiated PC12 cells using whole-cell patch-clamp techniques. RESULTS: The ACh-induced current (IAch) proved to be generated through neuronal nAChR. When ACh (30 μmol/L) was applied simultaneously with corticosterone (0.1 - 10μmol/L) , the decay of IAch was faster with slight inhibition...

To investigate the nongenomic effect of the glucocorticoid corticosterone on nicotinic acetylcholine receptor (nAChR) in PC12 cells. METHODS; The acetylcholine (ACh)-induced current was measured on nerve growth factor differentiated PC12 cells using whole-cell patch-clamp techniques. RESULTS: The ACh-induced current (IAch) proved to be generated through neuronal nAChR. When ACh (30 μmol/L) was applied simultaneously with corticosterone (0.1 - 10μmol/L) , the decay of IAch was faster with slight inhibition on the peak current amplitude. Pretreating PC12 cells with corticosterone augmented the inhibition on the peak IAchand did not alter receptor desensitization. Bovine serum albumin-conjugated corticosterone (0.1 - 10 /μmol/L) had the inhibition similar to corticosterone. The rapid effect induced by corticosterone was reversible, concentration-dependent, and voltage-independent. CONCLUSION: Corticosterone has rapid inhibitory effect on IAch which is mediated by a nongenomic mechanism. It indicates that corticosterone binds to the specific site on the outer cell membrane, probably on the neuronal nicotinic receptor-coupled channel, and inhibits the IAch in a noncompetitive manner, thus controlling the immediate catecholamine release from the sympathetic cells.

目的:研究糖皮质激素皮质酮对PC12细胞烟碱受体的非基因组作用.方法:采用全细胞膜片箝技术记录神经生长因子分化的PC12细胞上的乙酰胆碱(ACh)诱发电流(I_(ACh)).结果:PC12细胞上I_(ACh)是由烟碱受体引起的.同时给予ACh(3Oμmol/L)和皮质酮(0.1-10μmol/L)加速I_(ACh)的衰减且轻微抑制I_(ACh)峰值.用相同浓度的皮质酮预处理细胞可加强对I_(ACh)峰值的抑制率而不影响受体失敏速率.牛血清白蛋白耦联的皮质酮(0.1-10μmol/L)对I_(ACh)有类似于皮质酮的快速抑制作用.此快速抑制作用呈可逆性、浓度依赖性和非电压依赖性.结论:皮质酮可快速抑制PC12细胞I_(ACh),已是由非基因组机制介导的.皮质酮结合于膜外的特异结合位点,很可能直接作用于神经元烟碱受体,以一种非竞争方式抑制I_(ACh),从而调控交感神经元的儿茶酚胺释放.

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关corticosterone binding的内容
在知识搜索中查有关corticosterone binding的内容
在数字搜索中查有关corticosterone binding的内容
在概念知识元中查有关corticosterone binding的内容
在学术趋势中查有关corticosterone binding的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社