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hybridization combined
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  杂交组织化学
     Methods Situ hybridization combined with computer image analysis was used to exame the expression, volume density (Vv) and numerical density (Nv) of the SSmRNA positive cells in three groups of model rats, ie the regular electric and irregular flashing light stimulating group (group A), the regular electric and regular flashing light stimulating group (group B) and the only irregular flashing light stimulating group (group C).
     方法 采用心理应激大鼠模型A、B、C三组, 以原位杂交组织化学和微机图像分析技术, 检测大鼠心理应激后胃窦粘膜生长抑素mRNA 阳性反应细胞的表达强度和细胞体积密度(Vv) 、单位体积数密度(Nv) 的变化。
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  “hybridization combined”译为未确定词的双语例句
     The mutation of mismatch repair genes is also reported to be related to this process. We selected four genes which have significant different expression in cancer tissue compared to normal tissue from differentially expressed 86 genes by methods of suppression subtractive hybridization combined with cDNA microarray. They are gene of heat shock lOkDa protein 1 (HSPE1 HSP10) , SRY-box9 (SOX9), polymeric immunoglobulin receptor ( PIGR) and eukaryotic translation elongation factor 1 delta , EEF1 —delta.
     我们利用抑制性消减杂交结合cDNA芯片技术,筛选出86个差异表达的基因片段,选择其中四个基因,分别为heat shock 10kDa protein 1(HSPE1 HSP10)基因、SRY-box9(SOX9)基因、polymeric immunoglobulin receptor(PIGR)和真核细胞延长因子—1 delta(eukaryotic translation elongation factor1 delta,EEF1—delta)基因。
短句来源
     Methods By means of in situ hybridization combined with the micro-image analysis to investigate the changes of tumor necrosis factor-α mRNA(TNF-α mRNA) expression and the inhibitory effect of the antibody against NGF in lower respiratory tract and viscerosensory afferent system of experimental asthmatic guinea pigs.
     方法 应用原位杂交方法结合显微图像分析 ,研究实验性哮喘豚鼠下呼吸道和内脏感觉传入部位肿瘤坏死因子 αmRNA(TNF αmRNA)表达的变化以及NGF抗体对其的影响。
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     Methods The multiple PCR and in situ hybridization combined with the pathohistological study were used for the detection of HPV DNA in 100 cases of esophageal carcinomas and 49 cases lung cancers.
     方法 用 PCR法和原位杂交法检测 1 0 0例食管鳞癌和 49例肺鳞癌组织中的 HPV DNA并结合病理组织学研究。
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     METHODS: The quantitative method of immuno-cytochemistry and in situ hybridization combined with cytokine bioassay were used to detect the expression of endogenous OFQ and ORLl and the production of interleukin-1 (IL-1) and tumor necrosis factor-α ( TNF-α) from peritoneal macrophage.
     方法:采用免疫组织化学,原位杂交,及细胞因子的生物活性检测技术,定量分析内源性孤啡肽及其受体在中枢神经系统的表达及腹腔巨噬细胞分泌IL-1及TNF-α的能力.
短句来源
     In situ hybridization combined with immunohistochemistry were employed to explore whether the PrRP receptor mRNA co-localize with GnRH.
     通过原位杂交结合免疫组织化学的方法,检测下丘脑GnRH阳性神经元(纤维)与PrRP受体mRNA是否共存;
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  相似匹配句对
     Combined wiih S.D.
     运用灰色局势决策与S.
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     B.Chao has been combined as Q.
     B.Chao新组合为一变型;
短句来源
     INDIRECT HYBRIDIZATION
     间接核酸杂交方法的建立
短句来源
     northern hybridization.
     Northern杂交。
短句来源
     Detection and identification of fungi with clinical significance by PCR combined with oligonucleotide probe hybridization
     PCR结合寡核苷酸探针杂交检测临床常见真菌的实验研究
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  hybridization combined
A one-day double-labelling technique for tissue specimens: Immunogold-silver staining forin situ hybridization combined with alk
      
In situ hybridization combined with banding showed that ferret Chromosome (Chr) 1 = cat A2p/C2, Chr 2 = F2/C1q, and Chr 3 = A2q/D2.
      
In situ hybridization combined with immunocytochemistry for IAPP, revealed labelling of the IAPP-immunoreactive cells.
      
A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described.
      
In situ hybridization combined with anti-CT immunocytochemistry showed a low expression of α2 mRNA on differentiated C cells and thyrocytes, but an overexpression on immunonegative spontaneous MTC and induced intrathyroid tumours.
      
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This paper reports the breeding procedure of the new soybean culti-var, Heinong26, its utilization in production after releasing, and its economical benifits. The reasons of its rapid expansion is, in addition to that it wins the welcame of the farmer with its good properties, a timely miltiplication is also important.Basing upon the role played by Heinong26 in production and its morphological characteristics, the objectives of soybean breeding in the south-central area of Heilovngjang proince are d scussed.Results...

This paper reports the breeding procedure of the new soybean culti-var, Heinong26, its utilization in production after releasing, and its economical benifits. The reasons of its rapid expansion is, in addition to that it wins the welcame of the farmer with its good properties, a timely miltiplication is also important.Basing upon the role played by Heinong26 in production and its morphological characteristics, the objectives of soybean breeding in the south-central area of Heilovngjang proince are d scussed.Results show that the method of hybridization combined with radiation is comparatively more effective in soybean breeding at present.Jn order to shorten the time needed for developing a soybean cultiva still further, a modified method of improving the handling of hybrid generations in Hainan island is suggested.

本文报导了大豆新品种“黑农26”的选育过程,推广后的应用情况和经济效益。分析该品种推广快的原因,除品种本身的优良特性受农民欢迎外,及时抓紧种子繁殖也是重要的一环。 根据“黑农26”在生产上所起的作用及其形态特征,讨论了我省中南部地区大豆育种的近期目标。杂交与辐射相结合是当前比较有效的一种育种途径。对在海南岛繁殖后代,缩短育种年限的方法,提出改进意见。

Biotin-labeled probes were enzymatically prepared from allele specific 19mer oligonucleotides by the use of biotin-11-dUTP and terminal deoxynucleotidyl transferase (TdT) . Only one biotin molecule was transfered to the 3' end of a 19mer. the specificity of biotinylated probe was comparable to 32P labeled one and their hybrization sensitivity was corresponding to about 2-3pg of the related sequence in target DNA.A DNA sample from white blood cells of HbS heterozygote was amplified by polymerase chain reaction...

Biotin-labeled probes were enzymatically prepared from allele specific 19mer oligonucleotides by the use of biotin-11-dUTP and terminal deoxynucleotidyl transferase (TdT) . Only one biotin molecule was transfered to the 3' end of a 19mer. the specificity of biotinylated probe was comparable to 32P labeled one and their hybrization sensitivity was corresponding to about 2-3pg of the related sequence in target DNA.A DNA sample from white blood cells of HbS heterozygote was amplified by polymerase chain reaction (PCR) . The amplified fragments were detected by the biotinylated 19mer probes (β6S and β6A) on dot blots, using the 32P-labeled 19mer probes as control. The hybridization signal was developed in the heterozygote DNA both with normal and mutant probes whereas the signal for the normal β-globin gene DNA was detected only with the β6A probe It showed that the biotinylated oligonucleotide probe hybridization combined with PCR would be able to detect some genetic diseases through a simple, accurate and nonradioactive dot blotting technique.

用末端转移酶催化生物素核苷酸底物(Biotin-ll-dUTP)共价连接在合成的寡核苷酸3’羟基末端,从而合成了两种寡核苷酸探针(β~T_(41-42)及β~A_(41-42))。用它们分别与克隆化扩增的正常和突变的β—珠蛋白基因片段杂变。结果表明该探针都具有与~(32)P探针相似的特异性,其杂交的灵敏度为2—3pg(特异序列)。进而将探测HbS基因的正常和异常两种寡核苷酸19聚体(β~A_6和β~S_6)用~(32)P和生物素分别标记;将HbS杂合子病人的白细胞DNA经聚合酶链反应(PCR)法扩增,并以含正常β—珠蛋白基因的DNA片段作对照,与两种探针分别进行斑点杂交。所得结果完全一致;Hbs杂合子DNA对正常和异常探针都显出杂交信号,而正常DNA只与β~A探针显杂交信号。

n oligonucleotide probe directed against small RNAs encoded by EBV gene was used to detect EBV genome by in situ hybridization combined with immunophenotype analysis. The results showed that(1) in 18 of 19 cases(94.7%)atypical lymphoid cells(ALC)expressed T-cell differentiation antigen,15 cases were CD3 positive,9 cases were UCHL1 positive,6 cases were both CD3 and UCHL1 positive,no ALC gave positive reaction to B-cell and histiocvte markers.(2)15 of 19 cases gave positive reaction to EBER fsmall RNAs...

n oligonucleotide probe directed against small RNAs encoded by EBV gene was used to detect EBV genome by in situ hybridization combined with immunophenotype analysis. The results showed that(1) in 18 of 19 cases(94.7%)atypical lymphoid cells(ALC)expressed T-cell differentiation antigen,15 cases were CD3 positive,9 cases were UCHL1 positive,6 cases were both CD3 and UCHL1 positive,no ALC gave positive reaction to B-cell and histiocvte markers.(2)15 of 19 cases gave positive reaction to EBER fsmall RNAs encoded by EBV gene)by in situ hybridization analysis and A LC were reactive to T-cell markers in all 15 cases but one. The resuIts of this study suggest that EBV infection is also present in midline malignant reticulosis, whlch is characterized by active T-cell proliferation. The role and effect of EBV infection in pathogenesis of lymphoproliferative idsorders was discussed.

使用了一个针对EB病毒编码的小分子RNA(EBER)的寡核苷酸探针对19例中线恶性网织细胞增多症病变组织进行了原位杂交检测,并配合免疫表型分析。结果为:(1)本组病例中18/19例(94.7%)的病变组织中的异形淋巴样细胞表达T细胞分化抗原,CD3阳性15例,UCHL1阳性9例,其中二者均阳性6例,但各例中的异形淋巴样细胞均未表达B细胞及组织细胞分化抗原;(2)EBER原位杂交检侧的阳性数高(15/19例,78.9%),而其中14例的异形淋巴样细胞表达了T细胞分化抗原。结果表明:EB病毒感染也存在于以T淋巴细胞的活跃增殖为突出表现的中线恶网病变组织中。还对EB病毒感染在有关的淋巴增生性疾病发病学中的地位和作用等问题进行了讨论。

 
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