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target rna
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  靶rna
     The 32 P labled caspase 3 transcript being target RNA, cleavage reaction in vitro showed that pM1 GS716 and pM1 GS337 were active, the extent of pM1 GS716 cleavage was 93%.
     3 2 P标记的caspase 3基因片段体外转录物作为靶RNA ,体外切割实验表明 ,pM1 GS716和pM1 GS337均有切割活性 ,其中pM1 GS716的切割效率可达到 93% .
短句来源
     Computer analysis of the interaction of ribozyme and target RNA
     抗HPV16E7-ribozyme和靶RNA相互作用的计算机分析
短句来源
     Methods The fragments of the second exons of pro alpha 1Ⅰand Ⅲcollagen genes were cloned in-to the plasmids pT-Ⅰand pT-Ⅲand labeled with 32 P during transcription to obtain the target RNA.
     方法将含α1Ⅰ型及Ⅲ型前胶原基因第2外显子片段的重组质粒(pT-Ⅰ、pT-Ⅲ),经体外32P标记转录后形成产物靶RNA
短句来源
     After in vitro transcription, the cleavage reaction was shown to have cut off 79.3% target RNA in 1h.
     经体外转录出核酶RZ1及靶RNA后,体外切割反应1小时,79.3%的靶RNA即被切开。
短句来源
     Conclusions The anti-PDGFR- β ribozyme can be expressed stably in HSCs, cleave the target RNA effectively, inhibit HSCs proliferation and collagen synthesis, and induce HSC apoptosis.
     结论 抗PDGFR-β核酶的真核表达载体可在细胞内稳定表达,能有效切割靶RNA,抑制HSC增殖及胶原合成,并诱导其凋亡。
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  “target rna”译为未确定词的双语例句
     Result: A single and prospective band of CYP1A1, CYP1A2, CYP2B1/B2, CYP2C11, CYP2E1, CYP4A1 and cyclophilin was observed after polymerase chain reaction (PCR) when the reactive system of reverse transcription (RT) had no target RNA, which confirmed the competi tior had a specific capacity to bind to the CYP or cyclophilin primer.
     结果:反转录反应体系不含目的RNA时,用参考模板竞争RNA反转录后用CYP和看家基因Cyclophilin引物进行PCR,CYP1A1,CYP1A2,CYP281/B2,CYP2C11,CYP2E1,CYP3A1,CYP4A1,Cyclophilin均出现了其预期的特异性片段,无其他非特异性带出现,表明参考模板竞争RNA对CYP和看家基因Cyclophilin引物具有特异性竞争能力。
短句来源
     It showed that only Rz464 could cleave target RNA exclusively, then it was cloned into the pIRES2-EGFP vector for intracellular analyses (pIRES2-EGFP-Rz464, pRz464). Stable transfectants of Raji cell by pRz464 were detected for classical MHCⅡ (HLA-DR, -DP, -DQ) expression by flow cytometry, and the mRNA of CⅡTA was detected by RT-PCR.
     将切割作用明显的Rz464 亚克隆入真核表达载体pIRES2 EGFP,成为pIRES2 EGFP Rz464(pRz464),将pRz464稳定转染Raji细胞株,流式细胞术检测Raji细胞表面MHCⅡ类抗原(HLA DR、DP、DQ)的表达,并用逆转录聚合酶链反应分析Raji细胞CⅡTA mRNA的表达。
短句来源
     Results A 198 bp GPI-PLD competitive RNA was constructed and prepared. The competitive RNA could compete well with the target RNA in the RT-PCR reaction.
     结果 :构建和制备了长度为 198bp的GPI PLD竞争性RNA模板 ,该模板与靶模板呈明显的竞争性RT PCR反应 ;
短句来源
     GS was designed to the target the mRNA encoding the major DNA polymerase of human cytomegalovirus(HCMV) for degradation. Covalent attachment to the 3′ end of M1 RNA of the guide sequence to DNA polymerase mRNA(M1GS-T7) results in very efficient cleavge of the target RNA in vitro.
     针对人巨细胞病毒HCMV(humancytomegalovirus)DNA聚合酶mRNA序列设计GS ,共价结合到大肠杆菌来源M1RNA中 ,构建成M1GS T7核酶。
短句来源
     Method Hammerhead ribozyme against CⅡTA recognizing at 134, 218 and 464 sites (Rz134, Rz218, Rz464 respectively) and CⅡTA target RNA were constructed, then cloned into the pGEM-T vector respectively. The recombinant ribozymes and their CⅡTA target RNA were incu -bated in cell-free conditions.
     方法 设计并克隆针对MHCⅡ类分子转录激活因子(CⅡTA)第134、218、464位点的3 个核酶片段(Rz134、Rz218、Rz464)及其相应的CⅡTA靶基因,分别插入pGEM T载体,进行细胞外切割活性筛选。
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  相似匹配句对
     N TARGET
     命中目标
短句来源
     Progress of RNA Target Sites Screening
     mRNA靶点筛选方法研究进展
短句来源
     Progress on RNA as Small Molecular Drug Target
     小分子药靶——RNA药靶研究进展
短句来源
     RNA editing
     RNA编辑
短句来源
     RNA interference
     RNA干涉技术
短句来源
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  target rna
Both ribozymes were first assayed on a synthetic 16 bases target RNA and found to catalytically and efficiently cleave the substrate in a sequence specific way.
      
In this chapter we describe methodologies to first analyze the ribozyme catalytic activity in vitro by studying cleavage of target RNA in vitro.
      
The behavior of class II mutants requires that exogenous 5FU is specifically excluded from the site of synthesis of the target RNA involved in petite mutagenesis, while 5FC has access to it.
      
In several systems, silencing has been found to spread from the dsRNA inducer sequence into upstream or downstream regions of the target RNA, a phenomenon termed transitive silencing.
      
The target RNA species may be the products of transgenes, endogenous plant genes or viral RNAs.
      
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ammerhead ribozyme recognizes and cleaves target RNA by base pairing to sequences of both sides ofcleavage site on target RNA. The effect of the interaction between ribozyme and forget RNA on catulyticefficiency was studied by changing the length of base pairs and the property of double strands in the baseP8iring region. Results revealed that shortening the length of base pairs and changing property in basepairing region caused the decrease of melting temperature and optimal temperature., that...

ammerhead ribozyme recognizes and cleaves target RNA by base pairing to sequences of both sides ofcleavage site on target RNA. The effect of the interaction between ribozyme and forget RNA on catulyticefficiency was studied by changing the length of base pairs and the property of double strands in the baseP8iring region. Results revealed that shortening the length of base pairs and changing property in basepairing region caused the decrease of melting temperature and optimal temperature., that km valueincreased gradually as temperature went up and increased greatly as temperature was close to or higher thanTin, and that heat increased as temperature went up while the increase rate of heat slowed down and finallydecreased.

锤头结构Ribozyme(Rz)通过与靶RNA上切卢、二狈帕5核普酸序列的碱基配对识别和切割靶RNA。通过改变Ribozyme与靶RNA之间碱基配对的长度以及性质,研究了Ribozyme与靶RNA相互作用对于它的切割反应的影响。结果表明:(1)缩短碱基配对长度和改变碱基配对的性质,使Ritiozyme与底物的熔卢、(Tm)降低,同时反应最适温度(Topt)也降低。(2)动力学分析表明,反应的Km值随温度升高而增加,当温度接近或高于Ribozyme与底物的熔点时,Km值增加大大加快。(3)反应的Kcat也随温度升高而增加,但Kcat增加的速度继而逐渐变慢,最后Kcat下降。

We have analyzed in vitro cleavage reactions of anti-HPV16 E7-ribozyme by computer program.The results demonstrate that secondary structure and energy changes of ribozyme and target RNA affectthe cleavage activity of ribozyme. According to the hypothetical energy profile for hammerhead ribozyme,we also analyze the interaction of this ribozyme and target RNA in vaccinia-expression system and stable-expression system. It is implicated that we can conduct the design of ribozyme and its application in...

We have analyzed in vitro cleavage reactions of anti-HPV16 E7-ribozyme by computer program.The results demonstrate that secondary structure and energy changes of ribozyme and target RNA affectthe cleavage activity of ribozyme. According to the hypothetical energy profile for hammerhead ribozyme,we also analyze the interaction of this ribozyme and target RNA in vaccinia-expression system and stable-expression system. It is implicated that we can conduct the design of ribozyme and its application in vivo bymeans of computer analysis.

应用计算机分析了抗HPV16E7-ribozyme的体外切割反应结果,发现ribozyme和靶RNA的二级结构及其能量变化能影响ribozyme的切割活性。根据锤头结构ribozyme的能量变化模型,对抗HPV16E7-ribozyme和靶RNA在痘苗表达体系及稳定表达体系中的相互作用,进行计算机模拟分析。结果表明,计算机分析ribozyme和靶RNA在细胞内的相互作用,可以指导ribozyme的设计和在体内的应用研究。

Based on the previous studies with the antisense RNA techique that the inhibitory effect on HBV gene expression came from the 5'-end and non-coding region of the P gene, a hammerhead ribozyme (RCP) was synthesized according to the ribozyme structural model published by Haseliff and Gerlach. This ribozyme targeted at the 5'-end of the P gene at 2360 site (GUC) on HBV ayw genome. It was found that RCP successfully cleaved its target RNA (340 nucleotide nt) and produced 2 specific degradation fragments....

Based on the previous studies with the antisense RNA techique that the inhibitory effect on HBV gene expression came from the 5'-end and non-coding region of the P gene, a hammerhead ribozyme (RCP) was synthesized according to the ribozyme structural model published by Haseliff and Gerlach. This ribozyme targeted at the 5'-end of the P gene at 2360 site (GUC) on HBV ayw genome. It was found that RCP successfully cleaved its target RNA (340 nucleotide nt) and produced 2 specific degradation fragments. This finding provides a basis for the further study of its blocking effect on HBV gene expression and replication in vivo.

Effectsofhammerheadribozyme(RCP)onHBVPgeneinvitroGanLixia(甘立霞),WangPing(王平),ZhuXihua(朱锡华),DongYanlin(董燕麟),HouYunde(侯云德)(Depar...

 
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