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target rna
相关语句
  靶rna
    Computer analysis of the interaction of ribozyme and target RNA
    抗HPV16E7-ribozyme和靶RNA相互作用的计算机分析
短句来源
    We have analyzed in vitro cleavage reactions of anti-HPV16 E7-ribozyme by computer program. The results demonstrate that secondary structure and energy changes of ribozyme and target RNA affectthe cleavage activity of ribozyme.
    应用计算机分析了抗HPV16E7-ribozyme的体外切割反应结果,发现ribozyme和靶RNA的二级结构及其能量变化能影响ribozyme的切割活性。
短句来源
    Methods The fragments of the second exons of pro alpha 1Ⅰand Ⅲcollagen genes were cloned in-to the plasmids pT-Ⅰand pT-Ⅲand labeled with 32 P during transcription to obtain the target RNA.
    方法将含α1Ⅰ型及Ⅲ型前胶原基因第2外显子片段的重组质粒(pT-Ⅰ、pT-Ⅲ),经体外32P标记转录后形成产物靶RNA
短句来源
    PART I Selection of Binding Sites and Design of Specific DZ against Both Subgroups of RSVThree main basic factors should be considered for DZ cleavage site selection against the target RNA.
    靶RNA中DZ切割位点的选择主要考虑三个因素。
短句来源
    According to the hypothetical energy profile for hammerhead ribozyme,we also analyze the interaction of this ribozyme and target RNA in vaccinia-expression system and stable-expression system.
    根据锤头结构ribozyme的能量变化模型,对抗HPV16E7-ribozyme和靶RNA在痘苗表达体系及稳定表达体系中的相互作用,进行计算机模拟分析。
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  “target rna”译为未确定词的双语例句
    Conclusion: NHE 1 hammerhead ribozyme can cleave the target RNA specifically, reduce the expression of NHE 1 mRNA, induce intracellular acidosis and consequently prohibit the proliferation of PASMCs.
    结论 :NHE 1特异性锤头状核酶可对NHE 1mRNA进行特异性切割 ,减少其表达 ,从而诱导细胞酸化 ,抑制肺动脉平滑肌细胞增殖
短句来源
    Method Hammerhead ribozyme against CⅡTA recognizing at 134, 218 and 464 sites (Rz134, Rz218, Rz464 respectively) and CⅡTA target RNA were constructed, then cloned into the pGEM-T vector respectively. The recombinant ribozymes and their CⅡTA target RNA were incu -bated in cell-free conditions.
    方法 设计并克隆针对MHCⅡ类分子转录激活因子(CⅡTA)第134、218、464位点的3 个核酶片段(Rz134、Rz218、Rz464)及其相应的CⅡTA靶基因,分别插入pGEM T载体,进行细胞外切割活性筛选。
短句来源
    It showed that only Rz464 could cleave target RNA exclusively, then it was cloned into the pIRES2-EGFP vector for intracellular analyses (pIRES2-EGFP-Rz464, pRz464). Stable transfectants of Raji cell by pRz464 were detected for classical MHCⅡ (HLA-DR, -DP, -DQ) expression by flow cytometry, and the mRNA of CⅡTA was detected by RT-PCR.
    将切割作用明显的Rz464 亚克隆入真核表达载体pIRES2 EGFP,成为pIRES2 EGFP Rz464(pRz464),将pRz464稳定转染Raji细胞株,流式细胞术检测Raji细胞表面MHCⅡ类抗原(HLA DR、DP、DQ)的表达,并用逆转录聚合酶链反应分析Raji细胞CⅡTA mRNA的表达。
短句来源
    Among the family of catalytic DNAs, those with the highly-conserved motif of 5'-GGCTAGCTACAACGA-3', which acts as the active center and contributes to the ability of cleaving target RNA site-specifically, have caught the great attention of scientists for their potential usage as the tools of gene modulation or therapy.
    其中,活性中心为5'-GGCTAGCTACAACGA-3',能特异性剪切RNA分子的脱氧核酶在基因调控中的潜在应用价值尤为引人注目。
短句来源
    Ribozyme is a kind of small RNA molecule with catalytic function that can bind to specific target RNA via formation of base pairs and then cleave it at the specific site.
    核酶是一种具有催化作用的小RNA分子,能与特异性RNA以碱基配对方式结合并使其在特定核苷酸部位发生裂解。 在所有核酶形式中,锤头样核酶是最小最有特异性的,被研究得也最多。
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  target rna
Both ribozymes were first assayed on a synthetic 16 bases target RNA and found to catalytically and efficiently cleave the substrate in a sequence specific way.
      
In this chapter we describe methodologies to first analyze the ribozyme catalytic activity in vitro by studying cleavage of target RNA in vitro.
      
The behavior of class II mutants requires that exogenous 5FU is specifically excluded from the site of synthesis of the target RNA involved in petite mutagenesis, while 5FC has access to it.
      
In several systems, silencing has been found to spread from the dsRNA inducer sequence into upstream or downstream regions of the target RNA, a phenomenon termed transitive silencing.
      
The target RNA species may be the products of transgenes, endogenous plant genes or viral RNAs.
      
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We have analyzed in vitro cleavage reactions of anti-HPV16 E7-ribozyme by computer program.The results demonstrate that secondary structure and energy changes of ribozyme and target RNA affectthe cleavage activity of ribozyme. According to the hypothetical energy profile for hammerhead ribozyme,we also analyze the interaction of this ribozyme and target RNA in vaccinia-expression system and stable-expression system. It is implicated that we can conduct the design of ribozyme and its application in...

We have analyzed in vitro cleavage reactions of anti-HPV16 E7-ribozyme by computer program.The results demonstrate that secondary structure and energy changes of ribozyme and target RNA affectthe cleavage activity of ribozyme. According to the hypothetical energy profile for hammerhead ribozyme,we also analyze the interaction of this ribozyme and target RNA in vaccinia-expression system and stable-expression system. It is implicated that we can conduct the design of ribozyme and its application in vivo bymeans of computer analysis.

应用计算机分析了抗HPV16E7-ribozyme的体外切割反应结果,发现ribozyme和靶RNA的二级结构及其能量变化能影响ribozyme的切割活性。根据锤头结构ribozyme的能量变化模型,对抗HPV16E7-ribozyme和靶RNA在痘苗表达体系及稳定表达体系中的相互作用,进行计算机模拟分析。结果表明,计算机分析ribozyme和靶RNA在细胞内的相互作用,可以指导ribozyme的设计和在体内的应用研究。

Based on the previous studies with the antisense RNA techique that the inhibitory effect on HBV gene expression came from the 5'-end and non-coding region of the P gene, a hammerhead ribozyme (RCP) was synthesized according to the ribozyme structural model published by Haseliff and Gerlach. This ribozyme targeted at the 5'-end of the P gene at 2360 site (GUC) on HBV ayw genome. It was found that RCP successfully cleaved its target RNA (340 nucleotide nt) and produced 2 specific degradation fragments....

Based on the previous studies with the antisense RNA techique that the inhibitory effect on HBV gene expression came from the 5'-end and non-coding region of the P gene, a hammerhead ribozyme (RCP) was synthesized according to the ribozyme structural model published by Haseliff and Gerlach. This ribozyme targeted at the 5'-end of the P gene at 2360 site (GUC) on HBV ayw genome. It was found that RCP successfully cleaved its target RNA (340 nucleotide nt) and produced 2 specific degradation fragments. This finding provides a basis for the further study of its blocking effect on HBV gene expression and replication in vivo.

Effectsofhammerheadribozyme(RCP)onHBVPgeneinvitroGanLixia(甘立霞),WangPing(王平),ZhuXihua(朱锡华),DongYanlin(董燕麟),HouYunde(侯云德)(Depar...

AIM: To detect the cleavage activity of single andconnected ribozymes. METHODS: Three single ribozymesand a specific connected ribozyme (Rz 123 ) containing three ribozymes against hepatitis B virus core gene were designedwith computer, Then constructed transctiptional vector ofthree single ribozymes and transctiptional vector (pGEMRz123 ) containing connected ribozymes (Rz123 ) and 5'. 3'self-splicing ribozymes. Observed the cleavage activity ofthree single and connected ribozymes on target RNA. RESULTS:...

AIM: To detect the cleavage activity of single andconnected ribozymes. METHODS: Three single ribozymesand a specific connected ribozyme (Rz 123 ) containing three ribozymes against hepatitis B virus core gene were designedwith computer, Then constructed transctiptional vector ofthree single ribozymes and transctiptional vector (pGEMRz123 ) containing connected ribozymes (Rz123 ) and 5'. 3'self-splicing ribozymes. Observed the cleavage activity ofthree single and connected ribozymes on target RNA. RESULTS: Three single antiviral ribozymes can cleave its targetRNA efficiently,and connected ribozyme was released properly from the transcript of pGEMRz123, following cleavage by5' and 3 cis-ribozymes. The result also demonstrated thatthree ribozymes of , connected ribozyme can not only cleave itstarget site respectively, but also worked cooperatively. theconnected ribozyme increased cleavage efficiency than the single ribozyme. CONCLUSION: Three single and connected ribozymes with computer design can cleave RNA of hepatitis Bvirus core gene. The conected ribozyme can cleave targetRNA combingly.

目的:探讨单一及多位点核酶对乙型肝炎病毒靶RNA的切割作用.方法:利用计算机辅助设计针对乙型肝炎病毒C基因的三个单一核酶及特异联合型核酶,构建三个核酶各自及三个核酶串联的自剪切转录载体(pGEMRz123),观察单一及串联核酶(Rz123)对靶RNA的切割作用.结果:构建的串联核酶自剪切转录载体体外转录后,在顺式核酶发生自剪切后可将目的核酶正确地释放出来,计算机设计的单一核酶体外可剪切靶RNA;串联核酶中的单一核酶不仅可单独作用,而且可联合切割,提高了单一核酶的剪切效率.结论:抗乙型肝炎病毒特异联合型核酶体外可联合切割靶RNA.

 
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