助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   rhoptry protein 的翻译结果: 查询用时:0.154秒
图标索引 在分类学科中查询
所有学科
基础医学
更多类别查询

图标索引 历史查询
 

rhoptry protein
相关语句
  棒状体蛋白
     Study on the Invasion-related Genes and 235kDa Rhoptry Protein in the Sporozoite of Plasmodium Yoelii
     约氏疟原虫子孢子侵入相关基因分析及235kDa棒状体蛋白研究
短句来源
     CLONING AND EXPRESSION OF GENES ENCODING THE RHOPTRY PROTEIN ANTIGEN 2 AND THE MAJOR SURFACE ANTIGEN 1 OF TOXOPLASMA GONDII
     编码刚地弓形虫棒状体蛋白2与主要表面抗原1基因的克隆和表达(英文)
短句来源
     Cloning and Expression of the Fused Gene of Rhoptry Protein ROP2 and Major Surface Protein P30 from Toxoplasma gondii
     弓形虫棒状体蛋白2和膜表面蛋白1融合基因的克隆与表达
短句来源
     Rhoptry protein 1 (ROP1) from Toxoplasma gondii was an important molecular related with the host cell penetrating.
     弓形虫棒状体蛋白 1(ROP1)是与虫体侵入相关的重要分子。
短句来源
     Construction of a recombinant plasmid harbouring the rhoptry protein 1 gene of Toxoplasma gondii and preliminary observations on DNA immunity
     弓形虫侵入相关分子棒状体蛋白1基因重组质粒的克隆及其DNA免疫研究(英文)
短句来源
更多       
  “rhoptry protein”译为未确定词的双语例句
     In vitro amplification of Toxoplasma gondii rhoptry protein 2 gene and construction of its eu- karyotic expression plasmid
     弓形虫ROP2基因的体外扩增及真核表达重组质粒的构建
短句来源
     EXPRESSION OF RHOPTRY PROTEIN 1 RELATED WITH PENETRATED ENHANCING FACTOR FROM TOXOPLASMA GONDII IN HEPG-2 CELLS
     弓形虫侵入相关分子ROP1在HepG-2细胞中的表达
短句来源
     Cloning and analysis of nucleotide sequences encoding rhoptry protein2 of Toxoplasma gondii
     弓形虫自然弱毒株棒状蛋白2基因克隆及序列分析
短句来源
     VACCINATING MICE WITH EUKARYOTIC EXPRESSION RECOMBINANT PLASMID DNA CODING RHOPTRY PROTEIN 1 GENE FROM TOXOPLASMA GONDII V.Constructing Eukaryotic Expression Recombinant Plasmid Containing Interferon Gamma Gene and Its Effects as a Genetic A
     含弓形虫ROP1基因真核表达重组质粒DNA免疫小鼠的研究Ⅴ.IFN-γ基因真核表达重组质粒的构建及其在DNA免疫中基因佐剂的作用
短句来源
     Objective To construct the eukaryotic expression recombinant plasmid, pcIFN γ, as a genetic adjuvant and observe the immune responses elicited by pcDNA3 rhoptry protein 1 (pc ROP1) combined with pcIFN γ against Toxoplasma gondii (T gondii) infection in mice Methods A fragment of the IFN γ gene was directly inserted into the pcDNA3 plasmid and identified by two restriction endonucleases digestion pcIFN and pcROP1 DNA was injected into the left leg muscle of mice at a dosage of 100?
     目的 构建IFN γ基因真核表达重组质粒作为基因佐剂 ,观察其与pcDNA3 ROP1(pc ROP1)重组质粒DNA共同免疫小鼠所诱导抗弓形虫免疫应答。 方法 将IFN γ基因片段定向插入真核表达载体pcDNA3,双酶切鉴定 ,获得pcIFN重组子 ;
短句来源
更多       
  相似匹配句对
     protein.
     protein。
短句来源
     ,protein etc.
     、蛋白质等控制其质量。
短句来源
     Cloning and analysis of nucleotide sequences encoding rhoptry protein2 of Toxoplasma gondii
     弓形虫自然弱毒株棒状蛋白2基因克隆及序列分析
短句来源
     Study on the Invasion-related Genes and 235kDa Rhoptry Protein in the Sporozoite of Plasmodium Yoelii
     约氏疟原虫子孢子侵入相关基因分析及235kDa棒状体蛋白研究
短句来源
查询“rhoptry protein”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  rhoptry protein
The extracted DNA was subsequently amplified using specific primers derived from Theileria heat shock protein hsp70, Theileria lestoquardi ms1-2 gene, Babesia rhoptry protein gene and piroplasms hyper variable region V4 of 18S rRNA gene.
      
Western blotting and immunofluorescence showed that the 22-kDa rhoptry protein is expressed in schizonts and merozoites but not in sporozoites.
      
A merozoite-specific 22-kDa rhoptry protein of the coccidium Eimeria nieschulzi (Sporozoa, Coccidia) is exocytosed in the parasi
      
RAMA appears to be an unusual rhoptry protein with roles in parasite invasion as well as rhoptry biogenesis.
      


Aim Cloning the gene coding rhoptry protein 1(ROP1)from Toxoplasma gondii ZS2 isolate to prepare for expression and DNA vaccinating with the recombinant plasmid,pcDNA3 ROP1.Methods Amplifying gene fragment coding ROP1 from the genomic DNA of T.gondii ZS2 isolate by means of polymerase chain reaction (PCR),the gene was inserted into cloning vector,pUC18.The inserted ROP1 gene was recombined with pcDNA3 eukaryotic expression vector by digesting with restrictive enzymes and linking reactions.The positive...

Aim Cloning the gene coding rhoptry protein 1(ROP1)from Toxoplasma gondii ZS2 isolate to prepare for expression and DNA vaccinating with the recombinant plasmid,pcDNA3 ROP1.Methods Amplifying gene fragment coding ROP1 from the genomic DNA of T.gondii ZS2 isolate by means of polymerase chain reaction (PCR),the gene was inserted into cloning vector,pUC18.The inserted ROP1 gene was recombined with pcDNA3 eukaryotic expression vector by digesting with restrictive enzymes and linking reactions.The positive colon was screed on LB plates containing ampicillin and identified by restrictive enzyme digestion and PCR amplification.Results The specific gene fragment ROP1 was amplified from genomic DNA of ZS2 T.gondii islate;Constructed pUC18 ROP1 and pcDNA3 ROP1 were constructed successfully and the further research will be carried out.

目的构建弓形虫ZS2株pcDNA3-ROP1真核表达重组质粒,为进一步表达及DNA免疫做准备。方法用PCR技术从弓形虫ZS2分离株的基因组DNA中扩增编码棒状体蛋白1(ROP1)的基因片段,重组入pUC18克隆载体。将pUC18-ROP1中的ROP1外源基因片段经酶切、连接等反应,亚克隆入pcDNA3真核表达载体,再经含氨苄LB培养基筛选,酶切、PCR鉴定。结果从ZS2株基因组DNA中扩增出特异的ROP1基因片段,克隆成功pUC18-ROP1;经亚克隆,筛选鉴定获得了pcDNA-ROP1重组质粒。结论构建成功弓形虫pUC18-ROP1重组质粒,亚克隆成功pcDNA-ROP1重组质粒,为下一步研究奠定了基础

Aim Construction of recombinant plasmid contained a gene encoding rhoptry protein 1 (ROP1) of Toxoplasma gondii and expression in E coli Methods Injecting mice with RH strain tachyzoites then harvesting infected ascites of mice,purifying tachyzoites and preparing genomic DNA One pair of primers were designed according to the sequence of ROP1 Using PCR technique,a fragment of ROP1 gene was obtained by amplification of genomic DNA of tachyzoites By cloning target gene into a high level expression...

Aim Construction of recombinant plasmid contained a gene encoding rhoptry protein 1 (ROP1) of Toxoplasma gondii and expression in E coli Methods Injecting mice with RH strain tachyzoites then harvesting infected ascites of mice,purifying tachyzoites and preparing genomic DNA One pair of primers were designed according to the sequence of ROP1 Using PCR technique,a fragment of ROP1 gene was obtained by amplification of genomic DNA of tachyzoites By cloning target gene into a high level expression vector,pBV220,a recombinant pBV220 ROP1 was constructed Transferring pBV220 ROP1 into E coli DH5 α,the nonfusion recombinant protein was expressed by temperature eliciting and was analyzed by means of SDS PAGE and Western blot Results The size of the amplification ROP1 gene fragment was in accordance with the expected one matelly inditing the recombinant pBV220 ROP1 was successfully constructed The results of SDS PAGE and Western blot revealed that the molecular weight of recombinant protein was approximately 43kD and can be specifically recognized by rabbit antiserum of Toxoplasma Conclusion The gene encoding ROP1 was amplified from genomic DNA of Toxoplasma and pBV220 ROP1 recombinant was successfully constructed The recombinant ROP1 protein was expressed in E coli and will be used to carry out further study in pathology and immunology of T gondii

目的构建弓形虫棒状体蛋白(ROP1)基因重组质粒并在E.coli中表达。方法用RH株接种小鼠,收集腹水,纯化速殖子,抽提基因组DNA;据ROP1基因序列设计合成一对引物,将上、下游引物分别引入EcoRI,BamHI酶切位点,用PCR技术从RH株基因组DNA中扩增编码ROP1的基因片段,插入pBV220质粒,转化大肠杆菌DH5α感受态细胞,于氨苄阳性LB培养平板上筛选阳性克隆,酶切鉴定;经温度诱导在E.coli中表达,SDS-PAGE及免疫印迹分析。结果ROP1基因体外扩增产物大小与预期值相符,约756bp;构建成功pBV220-ROP1重组质粒;SDS-PAGE、免疫印迹显示特异蛋白条带的分子量约43kD,表达产量约占菌体蛋白13.23%。结论从弓形虫基因组DNA中获取ROP1基因,并成功构建pBV220-ROP1重组质粒,诱导表达ROP1非融合蛋白,为进一步分离纯化、用于对弓形虫侵入机制及免疫特性的研究做好准备。

Rhoptry protein 1 (ROP1) from Toxoplasma gondii was an important molecular related with the host cell penetrating. To prepare for further study, the eukaryotic expression recombinant pcDNA3 ROP1 was introduced into mammalian cells, HepG 2, using lipsome mediated transfection. The transfected cell clones were selected by G418 culturing,and the cells and their cultured fluid were tested by SDS PAGE and Western blot. The results showed that figure of SDS PAGE and Western blot revealed that a...

Rhoptry protein 1 (ROP1) from Toxoplasma gondii was an important molecular related with the host cell penetrating. To prepare for further study, the eukaryotic expression recombinant pcDNA3 ROP1 was introduced into mammalian cells, HepG 2, using lipsome mediated transfection. The transfected cell clones were selected by G418 culturing,and the cells and their cultured fluid were tested by SDS PAGE and Western blot. The results showed that figure of SDS PAGE and Western blot revealed that a band about 35-40 kDa in the sample of pcROP1/HepG2 cell lysis could be recognized by rabbit antiserum of T. gondii . pcROP1/HEPG 2, and a stable expression system in vitro has been established and the antigenicity of the recombinant protein has been confirmed.

弓形虫棒状体蛋白 1(ROP1)是与虫体侵入相关的重要分子。将含编码 ROP1蛋白基因的真核表达重组质粒 pc DNA3转染 Hep G- 2细胞 ,为进一步研究做准备。用脂质体介导的真核细胞转染法 ,G418筛选阳性克隆 ,SDS-PAGE及 Western- blot分析鉴定表达产物。结果显示 ,在所筛选的克隆化生长的细胞裂解液样品电泳图谱中 ,有一大小约 35~ 40 k Da的特异电泳条带 ,该条带可被弓形虫免疫兔血清识别。本研究建立了体外稳定表达 ROP1的细胞系 ,证实了表达产物的免疫原性

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关rhoptry protein的内容
在知识搜索中查有关rhoptry protein的内容
在数字搜索中查有关rhoptry protein的内容
在概念知识元中查有关rhoptry protein的内容
在学术趋势中查有关rhoptry protein的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社