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signal transductors
相关语句
  信号传递成份
     EXPRESSION OF INTERLEUKIN 6 SIGNAL TRANSDUCTORS IN PATIENTS WITH DIFFUSE PROLIFERATIVE LUPUS GLOMERULONEPHRITIS
     IL-6信号传递成份在弥漫性增生性狼疮性肾炎肾小球中的表达
短句来源
  “signal transductors”译为未确定词的双语例句
     The effect of thymosin alpha 1 on expression profiles of signal transductors involving anti-infection in human lymphocytes
     胸腺肽α_1对人淋巴细胞抗感染相关的信号转导分子的表达
短句来源
     Objective To study the effect of CpG containing oligodeoxynucleotide(CpG-ODN) on the phenotypes,function and expression of signal transductors and activators of transcription(STAT) and suppressors of cell signaling(SOCS) of peripheral blood dendritic cells(DC) in patients with chronic hepatitis B(CHB).
     目的研究CpG寡核苷酸(CpG-ODN)对慢性乙肝患者(CHB)外周血树突细胞(DC)表型和功能、细胞因子信号传导分子(STAT)及其抑制因子(SOCS)表达的影响。
短句来源
     Objective To study the effect of thymosin alpha 1 on expression profiles of signal transductors involving anti-infection in human peripheral blood lymphocytes.
     目的 用基因微矩阵技术研究胸腺肽α1作用于人外周血淋巴细胞后 ,细胞内与抗感染相关的信号转导分子的基因表达谱变化。
短句来源
  相似匹配句对
     Signal Attenuators
     信号衰减器
短句来源
     transmission of signal;
     信号的传递 ;
短句来源
     EXPRESSION OF INTERLEUKIN 6 SIGNAL TRANSDUCTORS IN PATIENTS WITH DIFFUSE PROLIFERATIVE LUPUS GLOMERULONEPHRITIS
     IL-6信号传递成份在弥漫性增生性狼疮性肾炎肾小球中的表达
短句来源
     The effect of thymosin alpha 1 on expression profiles of signal transductors involving anti-infection in human lymphocytes
     胸腺肽α_1对人淋巴细胞抗感染相关的信号转导分子的表达
短句来源
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Objective To study the effect of thymosin alpha 1 on expression profiles of signal transductors involving anti-infection in human peripheral blood lymphocytes. Methods Human peripheral blood lymphocytes isolated from three health donors were cultured with PHA(1 μg/ml) for 48 hours and then divided into experimental group and control group. The experimental group was stimulated by Tα_1 (0.1 μg/ml) additionally. Thereafter, the total RNA were extracted by TRIzol TM and further purified for mRNA. The...

Objective To study the effect of thymosin alpha 1 on expression profiles of signal transductors involving anti-infection in human peripheral blood lymphocytes. Methods Human peripheral blood lymphocytes isolated from three health donors were cultured with PHA(1 μg/ml) for 48 hours and then divided into experimental group and control group. The experimental group was stimulated by Tα_1 (0.1 μg/ml) additionally. Thereafter, the total RNA were extracted by TRIzol TM and further purified for mRNA. The fluorescent cDNA probes were prepared by labeling experimental group mRNA and control mRNA with Cy5-dUTP and Cy3-dUTP respectively through reverse transcription. The microarray was then hybridized against the cDNA probe mixture and the fluorescent signals were laser scanned and analyzed. Results According screening standard, there were 61 genes differentially expressed after stimulation with Tα_1 with the Cy5/Cy3 ratio >2 or <0.5. Among those genes with functions related to anti-infection, CAML,PI3-K,TSP2,HM74,PIAS,PTP,PSTP genes were up-regulated, MEK2,MAP3K,Lok genes were down-regulated. Conclusions The anti-infection function of Tα_1 were related to these up-regulated and down-regulated genes.

目的 用基因微矩阵技术研究胸腺肽α1作用于人外周血淋巴细胞后 ,细胞内与抗感染相关的信号转导分子的基因表达谱变化。方法 取正常外周血 6 0 0ml分离淋巴细胞 ,实验组加Tα1(0 .1μg/ml)刺激 ,对照组不加Tα1。培养 4 8h后 ,收获细胞、抽提细胞总RNA并纯化mRNA。逆转录反应将对照组和实验组的cDNA分别标记上荧光分子Cy3和Cy5 ,与信号转导表达谱基因微矩阵杂交后激光扫描、图像分析。结果 筛选出 6 1种Cy5 /Cy3比值大于 2或小于 0 .5的差异表达基因 ,其中与感染相关的有CAML ,PI3 K ,TSP2 ,HM 74 ,PIAS ,PTP ,PSTP等 7种基因表达上调 ,MEK2 ,MAP3K ,Lok等 3种基因表达发生下调。结论 Tα1的直接抗感染作用与这些信号转导分子的基因表达变化有关。

Objective To study the effect of CpG containing oligodeoxynucleotide(CpG-ODN) on the phenotypes,function and expression of signal transductors and activators of transcription(STAT) and suppressors of cell signaling(SOCS) of peripheral blood dendritic cells(DC) in patients with chronic hepatitis B(CHB).Methods Monocytes isolated from peripheral blood of CHB and healthy volunteers were cultured and induced by granulocyte-monocyte colony stimulating factor(GM-CSF) and interleukin-4(IL-4).Then,CpG-ODN or TNF-α...

Objective To study the effect of CpG containing oligodeoxynucleotide(CpG-ODN) on the phenotypes,function and expression of signal transductors and activators of transcription(STAT) and suppressors of cell signaling(SOCS) of peripheral blood dendritic cells(DC) in patients with chronic hepatitis B(CHB).Methods Monocytes isolated from peripheral blood of CHB and healthy volunteers were cultured and induced by granulocyte-monocyte colony stimulating factor(GM-CSF) and interleukin-4(IL-4).Then,CpG-ODN or TNF-α were added to stimulate the immatured DC,their effects on the phenotypes and function of DC were evaluated.(The expression of) signaling molecules of STAT1,3,4,5,6 and SOCS1,3 in DC were detected by Western blotting.Results The(expression) rates of HLA-DR of DC in CHB with the treatment of CpG-ODN or TNF-α were obviously increased;Both the IL-12p70 expression and stimulating capacity of DC to allogenic T lymphocytes were improved significantly in CpG-ODN group and TNF-α group(P<0.01 and 0.05,respectively);However neither CpG-ODN nor TNF-α could improve the expression of CD1a.Both CpG-ODN and TNF-α enhanced the expression of intracellular(STAT1,4,6 and SOCS1),3 in DC of CHB,but not affect the expression of STAT3,5.Conclusions CpG-ODN,like TNF-α,has remarkable stimulatory effect on the impaired phenotype and function of peripheral blood DC in patients with chronic hepatitis B and the mechanism is potentially explained by regulating the expression of signal molecules of STAT1,4,6 and SOCS1,3 in DC.

目的研究CpG寡核苷酸(CpG-ODN)对慢性乙肝患者(CHB)外周血树突细胞(DC)表型和功能、细胞因子信号传导分子(STAT)及其抑制因子(SOCS)表达的影响。方法以细胞因子GM-CSF、IL-4自CHB和健康者外周血单核细胞诱导扩增DC,以CpG-ODN或TNF-α刺激DC,评价其对DC表型和功能的影响;应用W estern印迹法检测DC胞质STAT1、3、4、5、6以及SOCS1、3蛋白的表达。结果TNF-α、CpG-ODN能明显增强CHB患者DC的HLA-DR和IL-12 p70表达以及T细胞促增殖能力,但不能增强CD1 a的表达;两者能不同程度增强DC胞质STAT1、4、6和SOCS1、3的表达,但不影响STAT3、5的表达。结论CpG-ODN与TNF-α一样可能通过调节DC胞质信号分子STAT1、4、6及SOCS1、3的表达促进CHB外周血DC分化、成熟及其抗原递呈功能。

ObjectiveTo further study the effect of Janus kinase-signal transductors and activation of transcription(Jak-STAT) signal pathway in VSMCs proliferation induced by Ang Ⅱ. MethodsVSMCs were cultured and identified.Cells were activated with Ang Ⅱ at different times and verify its effect on VSMC proliferation by the method of bromodeoxyuridine incorporation.We extracted total protein and detected the activation and expression of nuclear factor-κB(NF-κB) and measured phosphorylation of Jak-STAT by immunoprecipitation,...

ObjectiveTo further study the effect of Janus kinase-signal transductors and activation of transcription(Jak-STAT) signal pathway in VSMCs proliferation induced by Ang Ⅱ. MethodsVSMCs were cultured and identified.Cells were activated with Ang Ⅱ at different times and verify its effect on VSMC proliferation by the method of bromodeoxyuridine incorporation.We extracted total protein and detected the activation and expression of nuclear factor-κB(NF-κB) and measured phosphorylation of Jak-STAT by immunoprecipitation, Western blot and immunofluorescence after cell disruption.Meanwhile we established negative group and different treatment groups.ResultsAng Ⅱ caused significant increases in BrdU incorporation during 6-30 h of cells proliferation with the peak value at 6 and 12 h.The OD value of in 490 nm was higher in Ang Ⅱ groups (0.590±0.029) than AG490+Ang Ⅱ groups(0.381±0.019), PDTC+Ang Ⅱ groups(0.481±0.024), losartan+Ang Ⅱ groups (0.519±0.026) and serum free groups(0.30±0.015),P<0.01.Western blotting showed that expression of NF-κB and phosphorylation Jak-STAT molecules in VSMCs induced by the Ang Ⅱ reached to peak value at 5, 15, 60 min respectively.Meanwhile Ang Ⅱ induced a time-dependent reversible translocation of STAT1 and NF-κB protein from cytoplasm to nuclei by immunofluorescence.The level of STAT1 in nuclei which was stimulated by Ang Ⅱ for 15 min was higher than that for 60 min stimulation (P<0.05) and controls (P<0.01).ConclusionWe have identified that Ang Ⅱ could improve proliferation of VSMCs and expression of NF-κB and phosphorylation Jak-STAT and change with time gradient in VSMCs.Our studies therefore suggested that Jak-STAT and NF-κB signal transduction pathways were activated and participated in the mechanism of VSMCs proliferation induced by Ang Ⅱ.

目的进一步探讨JakSTAT信号途径在血管紧张素Ⅱ介导的主动脉平滑肌细胞增殖效应的作用。方法培养大鼠主动脉平滑肌细胞并行免疫组织化学鉴定,用血管紧张素Ⅱ以不同时间梯度刺激传代培养的大鼠主动脉平滑肌细胞,采用BrdU法测定细胞增殖,裂解细胞后提取细胞总蛋白,分别经免疫共沉淀、Western印迹和细胞免疫荧光化学等方法分析胞内NFκB和Jak/STAT信号分子激活及表达的情况,行不同组间并与阴性对照组做对比。结果在6~30h的平滑肌细胞增殖实验中,6、12h时间段增值最明显;其中12h时AngⅡ组490nm处吸光度值(0.590±0.029)显著高于AG490+AngⅡ组(0.381±0.019),PDTC+AngⅡ组(0.481±0.024),氯沙坦+AngⅡ组(0.519±0.026)和SerumFree组(0.30±0.02),P<0.01。Western印迹显示AngⅡ组中NFκB及磷酸化的Jak2、STAT1分别在5、15、60min表达明显达高峰;免疫荧光则表明NFκB及STAT1分子随时间梯度可逆的由胞浆转至胞核,在AngⅡ刺激15min组中STAT1表达水平比对照组(P<0.01)及刺激60min...

目的进一步探讨JakSTAT信号途径在血管紧张素Ⅱ介导的主动脉平滑肌细胞增殖效应的作用。方法培养大鼠主动脉平滑肌细胞并行免疫组织化学鉴定,用血管紧张素Ⅱ以不同时间梯度刺激传代培养的大鼠主动脉平滑肌细胞,采用BrdU法测定细胞增殖,裂解细胞后提取细胞总蛋白,分别经免疫共沉淀、Western印迹和细胞免疫荧光化学等方法分析胞内NFκB和Jak/STAT信号分子激活及表达的情况,行不同组间并与阴性对照组做对比。结果在6~30h的平滑肌细胞增殖实验中,6、12h时间段增值最明显;其中12h时AngⅡ组490nm处吸光度值(0.590±0.029)显著高于AG490+AngⅡ组(0.381±0.019),PDTC+AngⅡ组(0.481±0.024),氯沙坦+AngⅡ组(0.519±0.026)和SerumFree组(0.30±0.02),P<0.01。Western印迹显示AngⅡ组中NFκB及磷酸化的Jak2、STAT1分别在5、15、60min表达明显达高峰;免疫荧光则表明NFκB及STAT1分子随时间梯度可逆的由胞浆转至胞核,在AngⅡ刺激15min组中STAT1表达水平比对照组(P<0.01)及刺激60min高(P<0.05)。结论AngⅡ能导致VSMC增殖,同时NFκB和磷酸化的Jak2,STAT1表达增加并随刺激时间梯度改变。因此可说明在NFκB和Jak/STAT信号通路激活参与了血管紧张素Ⅱ导致的主动脉平滑肌细胞增殖作用。

 
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