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gene functional research
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  基因功能研究
     It was developed recently and would be a very potential technology on gene functional research because of its discrepancy, efficiency and universality.
     以其特异性、高效性和广泛性的优势,已经逐渐成为一项极具潜力的基因功能研究技术。
短句来源
     Ac- cording to recent references and the products of microarray in China and abroad, the microarray technology and its applications in gene functional research, classification of diseases, gene diagnosis and drug discovery screen are reviewed.
     根据近几年文献结合国内DNA微阵列产品综述微阵技术及其在基因功能研究,疾病分型,基因诊断以及新药筛选中的研究应用进展。
短句来源
     BACKGROUND &OBJECTIVE: RNA interference (RNAi) is a new gene block ing technology that silences target gene at post-transcription level induced by the small interference RNA (siRNA). RNAi has been demonstrated great prospect i n gene functional research and gene therapy areas.
     背景与目的:RNA干扰(RNAinterference,RNAi)是由双链RNA介导的、在转录后mRNA水平关闭相应基因表达的新基因阻断技术,在基因功能研究、基因治疗方面已显示出巨大的前景。
短句来源
     RNA interference(RNAi)is a new gene blocking technology that silences target gene at post-transcription level induced by the small interference RNA(siRNA). RNAi has been demonstrated great prospect in gene functional research and gene therapy areas.
     RNA干扰(RNAi)是由双链RNA介导,在转录后mRNA水平关闭相应基因表达的新基因阻断技术,在基因功能研究、基因治疗方面已显示出巨大的前景。
短句来源
  研究基因功能
     RNAi can down-regulate gene expression in cell, which not only be a powerful tool in gene functional research, but also provides a new technique for gene therapy.
     RNA干扰可以简单、有效、特异地下调细胞中基因的表达,它不但是研究基因功能的一种有力工具,而且为特异性基因治疗提供了新的技术手段。
短句来源
  相似匹配句对
     Functional Redundancy of Gene.
     基因的功能冗余
短句来源
     Functional Research of LASS2 Gene
     人源性长寿保障基因LASS2的功能研究
短句来源
     Functional Analysis of the Baculovirus Chitinase Gene
     杆状病毒几丁质酶基因功能分析
短句来源
     Progress in the Functional Gene Research of Medicinal Plants
     药用植物功能基因
短句来源
     On Gene Patenting
     基因的专利问题
短句来源
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DNA microarray technology is a new biological technique that developing in a nearly years. Ac- cording to recent references and the products of microarray in China and abroad, the microarray technology and its applications in gene functional research, classification of diseases, gene diagnosis and drug discovery screen are reviewed.

DNA微阵列技术(DNA microarray technology)是近几年发展起来的应用 DNA微阵列进行基因功能研究的新的生物技术。根据近几年文献结合国内DNA微阵列产品综述微阵技术及其在基因功能研究,疾病分型,基因诊断以及新药筛选中的研究应用进展。

BACKGROUND &OBJECTIVE: RNA interference (RNAi) is a new gene block ing technology that silences target gene at post-transcription level induced by the small interference RNA (siRNA). RNAi has been demonstrated great prospect i n gene functional research and gene therapy areas. Nowadays, RNAi has been repor ted to be used to inhibit the expression of endogenous genes including cyclophil in, GAPDH, p53, and c-myc; and there were some progresses in the therapy of the diseases caused by AIDS and...

BACKGROUND &OBJECTIVE: RNA interference (RNAi) is a new gene block ing technology that silences target gene at post-transcription level induced by the small interference RNA (siRNA). RNAi has been demonstrated great prospect i n gene functional research and gene therapy areas. Nowadays, RNAi has been repor ted to be used to inhibit the expression of endogenous genes including cyclophil in, GAPDH, p53, and c-myc; and there were some progresses in the therapy of the diseases caused by AIDS and hepatitis viruses with RNAi. However, hTERT gene, w hich was highly expressed in hepatocellular carcinoma and other malignant neopla sm, has not been researched by RNAi. In present research, we utilized RNAi to in hibit hTERT gene expression in vitro and in vivo, investigated the feasibility a nd specificity of gene therapy for hepatocellular carcinoma. METHODS: Small inte rference RNAs homologous to hTERT gene were designed,pTZU6+1-shRNA-hTERT vect or was constructed and transfected into hepatocellular carcinoma SMMC-7721 cell s and transplanted SMMC-7721 tumor in nude mice to induce RNAi. The changes of hTERT gene expression and tumor cell proliferation in both siRNA treatment group s and control group were determined by flow cytometry, reverse transcription pol ymerase chain reaction (RT-PCR), immunochemistry in vitro and in vivo. RESULTS: The expression of hTERT had been obviously inhibited by RNAi in vitro. The inhi bition rate of cell growth was 37.5%after pTZU6+1-shRNA-hTERT vector was tra nsfected to hepatocellular carcinoma SMMC-7721 cells; the phase of cell cycle i ndicated the reduction of S phase, while G1/G0 phase increased. The mRNA level o f hTERT decreased from 99.4%to 53.1%, its protein expression reduced from 86.3 %to 46.6%. The tumor size reduced after treated with pTZU6+1-shRNA-hTERT ve ctor in vivo; hTERT mRNA level decreased from 99.1%to 76.2%, and its protein e xpression decreased from 87.2%to 61.8%in siRNA treatment group. In contrast, t here were no changes in control groups in vitro and in vivo. CONCLUSION: RNAi in hibits the hTERT gene expression and proliferation of hepatocellular carcinoma S MMC-7721 cells with specificity, and is a possible new approach for neoplasm ge ne therapy.

背景与目的:RNA干扰(RNAinterference,RNAi)是由双链RNA介导的、在转录后mRNA水平关闭相应基因表达的新基因阻断技术,在基因功能研究、基因治疗方面已显示出巨大的前景。目前,利用RNAi已抑制了包括cyclophilin、GAPDH、p53、c-myc在内的多个内源基因的表达。同时在艾滋病、病毒性肝炎等的治疗研究中也已取得一定进展。但对肝癌等恶性肿瘤中高表达的hTERT基因,国内外还未见相关研究报道。本研究利用RNAi技术,在体内外抑制hTERT基因表达,探讨RNAi对肝癌治疗的可行性。方法:设计干扰hTERT基因的小片段RNA,构建重组表达质粒pTZU6+1-shRNA-hTERT并导入肝癌SMMC7721细胞株和裸鼠移植瘤,在体内外诱导RNAi,采用流式细胞检测技术、RT-PCR法、免疫组化等同时检测RNAi治疗组和对照组hTERT基因表达及细胞增殖变化。结果:体外细胞实验显示,重组质粒pTZU6+1-shRNA-hTERT导入肝癌SMMC7721细胞株3~7天后,肝癌细胞生长抑制率达37.5%;细胞周期相分布发生显著变化,S期细胞明显减少,G1/G0期细胞显著增加;hTERT的mR...

背景与目的:RNA干扰(RNAinterference,RNAi)是由双链RNA介导的、在转录后mRNA水平关闭相应基因表达的新基因阻断技术,在基因功能研究、基因治疗方面已显示出巨大的前景。目前,利用RNAi已抑制了包括cyclophilin、GAPDH、p53、c-myc在内的多个内源基因的表达。同时在艾滋病、病毒性肝炎等的治疗研究中也已取得一定进展。但对肝癌等恶性肿瘤中高表达的hTERT基因,国内外还未见相关研究报道。本研究利用RNAi技术,在体内外抑制hTERT基因表达,探讨RNAi对肝癌治疗的可行性。方法:设计干扰hTERT基因的小片段RNA,构建重组表达质粒pTZU6+1-shRNA-hTERT并导入肝癌SMMC7721细胞株和裸鼠移植瘤,在体内外诱导RNAi,采用流式细胞检测技术、RT-PCR法、免疫组化等同时检测RNAi治疗组和对照组hTERT基因表达及细胞增殖变化。结果:体外细胞实验显示,重组质粒pTZU6+1-shRNA-hTERT导入肝癌SMMC7721细胞株3~7天后,肝癌细胞生长抑制率达37.5%;细胞周期相分布发生显著变化,S期细胞明显减少,G1/G0期细胞显著增加;hTERT的mRNA表达由99.4%下调到53.1%,hTERT蛋白表达由86.3%下调到46.6%。裸鼠体内实验结果显示,质粒pTZU6+1-shRNA-hTERT注射裸鼠皮下移植瘤7天后,瘤体积明显缩小,hTERT的mRNA表达由99.1%下调到76.2%,hTERT蛋白表达由87.2%

RNA interference (RNAi) is a post-transcriptional gene silencing induced by introduction of double-stranded RNA (dsRNA). RNAi can down-regulate gene expression in cell, which not only be a powerful tool in gene functional research, but also provides a new technique for gene therapy. This review introduces the enzymes, proteins, triggers, and regulators associated with mechanisms of RNAi, as well as the applications of RNAi in biology.

RNA干扰(RNAinterference,RNAi)是由双链RNA(doublestrandedRNA,dsRNA)引发的转录后基因沉默机制(posttranscriptionalgenesilencing,PTGS)[1]。RNA干扰可以简单、有效、特异地下调细胞中基因的表达,它不但是研究基因功能的一种有力工具,而且为特异性基因治疗提供了新的技术手段。本文介绍了目前RNA干扰作用机制,与之相关的酶、蛋白、“扳机点”和调节因子,及RNA干扰在生物学方面的应用。

 
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