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male protein
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  male蛋白抗原
     It was shown that both DC and MΦ can phagocytose, process rBCG·MalE, and present T cell epitopes of MalE protein to specific T hybridoma FBU·B11 using in vitro antigen presentation assay. Following infection of mice, DC and MΦ were purified and the presence of immunogenic peptide MHC class Ⅱ complexes could be detected ex vivo on sorted DC, but not on purified MΦ.
     体外抗原提呈试验结果显示 ,DC和MΦ能吞噬、加工rBCG·MalE并向T细胞杂交瘤FBU·B1 1提呈MalE蛋白抗原表位 ,而在体内试验中 ,从rBCG·MalE免疫小鼠脾脏中纯化的DC ,可检测到MalE表位MHCⅡ复合物 ,而纯化的MΦs却测不到。
短句来源
  “male protein”译为未确定词的双语例句
     Identification of T cell epitopes of MalE protein expressed by recombinant Mycobacterium bovis BCG
     重组卡介苗表达的MalE蛋白T细胞表位的鉴定
短句来源
     To address these questions, the rBCG expressingMalE protein of Escherichia coil (rBCG ?MalE) was used as modelstrain to study the mechanisms by which MalE protein is presentedby major histocompatibility complex class I (MHC I )molecules toT cells, to define the functional diversity of T cell epitopes ofexpressed MalE from rBCG, and to demonstrate the characteristicsand regulation mechanisms of Th responses initiated by rBCGMalE.
     为此,本研究以表达大肠杆菌MalE蛋白的rBCG(rBCG·MalE)为模型,试图阐明rBCG与抗原提呈细胞相互作用的规律、rBCG表达产物抗原表位多样性、rBCG和BCG诱导的T细胞应答规律与调控因素。
短句来源
     In order to determine the characteristics of T cell immune responses induced by recombinant Mycobacterium bovis BCG(rBCG)expressing MalE protein of Escherichia coli (rBCG·MalE),the splenocytes from immunized mice were treated to deplete CD4 + or CD8 + T cells by an immunomagnetic selection,then these cells were used to measure the IFN_γ of IL_2 responses to MalE protein or its peptides in enzyme linked immunospot (ELISPOT)assay.
     应用免疫磁性分离技术去除免疫小鼠脾脏细胞中CD4+或CD8+T细胞后 ,在酶联免疫斑点试验中证实表达大肠杆菌MalE蛋白的重组卡介苗 (rBCG·MalE)诱导的T细胞应答是CD4+T细胞依赖的。
短句来源
     The results clearly indicated that T cell immune responses induced by rBCG·MalE were CD4 + T cell_dependent. The results of analyzing T cell responses to MalE protein or PPD in different time points ofter rBCG·MalE immunization showed that initial Thl responses to rBCG or BCG were modulated during the course of infection to become a mixed Th1/Th2 responses.
     对MalE、PPD特异T细胞应答的动态分析结果表明 ,rBCG·MalE、BCG诱导的特异CD4+T细胞应答存在Th1/Th2平衡转换现象 ,即起始阶段为Th1应答 ,一段时间后出现Th2应答 ,并逐步形成Th1/Th2混合应答。
短句来源
     Four T cell epitopes -of MalEprotein presented on the surface of those DCs pulsed with rBCGMalE and purified MalE protein were recognized by CD4~ Thybridomas specific for MalE peptides p68? 2, p100? 14, p151?
     表位作图显示,BCG表达的MalE未形成新的抗原表位或使隐蔽表位暴露出来,并进一步证实MalE p68—82多肽表位是MalE H—2~d限制性主要T细胞表位,而p100—114、p151—165和p277—291多肽表位为次要表位。
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  相似匹配句对
     protein.
     protein。
短句来源
     male;
     男性;
短句来源
     PROTEIN REQUIREMENTS OF CHINESE MALE ADULTS
     我国成年男子蛋白质需要量的研究
短句来源
     The Inhibitor of Apoptosis Protein Survivin and Male Infertility.
     细胞凋亡因子survivin与男性不育症
短句来源
     Male Affairs
     男·性·事
短句来源
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  male protein
The male protein is expressed throughout feeding and it is present in nymphs and larvae.
      


In order to determine the characteristics of T cell immune responses induced by recombinant Mycobacterium bovis BCG(rBCG)expressing MalE protein of Escherichia coli (rBCG·MalE),the splenocytes from immunized mice were treated to deplete CD4 + or CD8 + T cells by an immunomagnetic selection,then these cells were used to measure the IFN_γ of IL_2 responses to MalE protein or its peptides in enzyme linked immunospot (ELISPOT)assay.The results clearly indicated that T cell immune responses...

In order to determine the characteristics of T cell immune responses induced by recombinant Mycobacterium bovis BCG(rBCG)expressing MalE protein of Escherichia coli (rBCG·MalE),the splenocytes from immunized mice were treated to deplete CD4 + or CD8 + T cells by an immunomagnetic selection,then these cells were used to measure the IFN_γ of IL_2 responses to MalE protein or its peptides in enzyme linked immunospot (ELISPOT)assay.The results clearly indicated that T cell immune responses induced by rBCG·MalE were CD4 + T cell_dependent.The results of analyzing T cell responses to MalE protein or PPD in different time points ofter rBCG·MalE immunization showed that initial Thl responses to rBCG or BCG were modulated during the course of infection to become a mixed Th1/Th2 responses.These results revealed new mechanisms in T cell immune responese against mycobacteria,and further showed that BCG is an effective live vector to express and deliver foreign antigens to host immune system.

应用免疫磁性分离技术去除免疫小鼠脾脏细胞中CD4+或CD8+T细胞后 ,在酶联免疫斑点试验中证实表达大肠杆菌MalE蛋白的重组卡介苗 (rBCG·MalE)诱导的T细胞应答是CD4+T细胞依赖的。对MalE、PPD特异T细胞应答的动态分析结果表明 ,rBCG·MalE、BCG诱导的特异CD4+T细胞应答存在Th1/Th2平衡转换现象 ,即起始阶段为Th1应答 ,一段时间后出现Th2应答 ,并逐步形成Th1/Th2混合应答。这些结果 ,为分枝杆菌T细胞应答规律提供了新的认识 ,同时 ,亦表明BCG是优良的外源抗原表达与运送载体。

Objective To identify T cell MalE epitopes protein by recombinant Mycobacterium bovis BCG(rBCG MalE). Methods The epitope repertoire of expressed MalE was analyzed in vitro by antigen presentation assay using dendritic cells as APC pulsed with rBCG.MalE, BCG wt or purified MalE protein, and further analyzed in vivo or in vitro using T cell proliferation assay, IFN-γ-ELISPOT and epitope mapping from immunized mice. Results rBCG MalE functionally expressed all 4...

Objective To identify T cell MalE epitopes protein by recombinant Mycobacterium bovis BCG(rBCG MalE). Methods The epitope repertoire of expressed MalE was analyzed in vitro by antigen presentation assay using dendritic cells as APC pulsed with rBCG.MalE, BCG wt or purified MalE protein, and further analyzed in vivo or in vitro using T cell proliferation assay, IFN-γ-ELISPOT and epitope mapping from immunized mice. Results rBCG MalE functionally expressed all 4 H-2 d restricted T cell epitopes of native MalE. Conclusion The p68-82 of expressed MalE was immunodominant epitope and the others were subdominant epitopes.

目的 鉴定重组卡介苗表达的MalE蛋白T细胞表位。方法 用抗原提呈试验、T细胞增殖试验、ELISPOT试验和表位作图测试重组MalE蛋白的T细胞表位。结果 重组BCG MalE功能性表达了MalE蛋白的 4种H 2 d 限制性T细胞表位。结论 在 4种表位中 ,p6 8~ 82是重组MalE蛋白的主要T细胞表位 ,而其余 3个为次要表位

The infection and antigen presenting cell functions of dendritic cells (DC) with recombinant BCG expressing MalE protein (rBCG·MalE ) from Escherichia coli were investigated. In both in vitro and in vivo assays, DC can capture rBCG·MalE. 1%~2% of the DC population was infected in vivo by the labeled rBCG·MalE through FACS analysis, a similar percentage of MΦ population was also found to be infected. It was shown that both DC and MΦ can phagocytose, process rBCG·MalE, and present...

The infection and antigen presenting cell functions of dendritic cells (DC) with recombinant BCG expressing MalE protein (rBCG·MalE ) from Escherichia coli were investigated. In both in vitro and in vivo assays, DC can capture rBCG·MalE. 1%~2% of the DC population was infected in vivo by the labeled rBCG·MalE through FACS analysis, a similar percentage of MΦ population was also found to be infected. It was shown that both DC and MΦ can phagocytose, process rBCG·MalE, and present T cell epitopes of MalE protein to specific T hybridoma FBU·B11 using in vitro antigen presentation assay. Following infection of mice, DC and MΦ were purified and the presence of immunogenic peptide MHC class Ⅱ complexes could be detected ex vivo on sorted DC, but not on purified MΦ. These results suggest that DC play a pivotal role on the initiation of protective immune responses against mycobacteria.

在体内外试验中 ,树突细胞 (DC)均能摄取表达大肠杆菌麦芽糖结合蛋白 (MalE)的重组卡介苗 (rBCG·MalE)。应用流式细胞术测得荧光标记的rBCG·MalE在体内感染DC的水平在 1 % 2 % ,与感染巨噬细胞 (MΦ)的水平相当。体外抗原提呈试验结果显示 ,DC和MΦ能吞噬、加工rBCG·MalE并向T细胞杂交瘤FBU·B1 1提呈MalE蛋白抗原表位 ,而在体内试验中 ,从rBCG·MalE免疫小鼠脾脏中纯化的DC ,可检测到MalE表位MHCⅡ复合物 ,而纯化的MΦs却测不到。这表明DC在起始抗分枝杆菌的免疫保护中发挥关键作用。

 
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