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covalently binding
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  共价结合
     【Methods】The magnetic beads covalently binding epithelial cell adhesion molecule (EpiCAM) antibody (Ab) can enrich the tumor cells in peripheral blood expressing EpiCAM , combined with the method of fluorescentimmunocytochemistry to detect the tumor cells expressing cytokeratin 8/18 (stained with green fluorescence) .
     【方法】通过与磁珠共价结合的表皮细胞黏附分子(EpCAM)抗体富集外周血中表达EpCAM的癌细胞,结合免疫荧光化学法检测细胞角蛋白(CK)8/18阳性的癌细胞(呈绿荧光)。
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  “covalently binding”译为未确定词的双语例句
     Compound 1 consists of one-dimensioinal zig-zag chains, which are constructed from [SiMoVIioMoV2O40(VIVO)2]2~ polyoxoanion clusters covalently binding to {Ni(phen)2} fragments through {VO} groups.
     化合物2和3是同构体。 在化合物2中,[SiMo_8~ⅥMo_4~ⅤO_(40)(V~ⅣO)_2]~(4-)多阴离子作为四齿配体,通过{VO}和{MoO}基团上的O原子与{Co(phen)_2}配合物片段共价连接形成二维层状结构。
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     were potential conjugating sites which had higher activities ,and that PEG whose molecular weight is 5000 doltans by proper linker could specifically bind to ε-amino group of lysines, which resulted in the conjugates whose molecular weight of is over 64KD. Moreover , PEG5000 with linker covalently binding to lysine residues of bHb could not influence the structure of oxygen binding in bHb/pHb.
     结果表明,bHb与pHb与二聚体中分别有12个和13个赖氨酸残基的氨基具有较高反应活性,可以作为进行修饰部位,与5KD的PEG-Linker形成的共轭物将大于64KD,但不影响血红蛋白的携氧功能。
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     The optimum pH for covalently binding the enzyme was 7.2 in 0.1 M tris buffer. The relative activity of the immobilized alkaline phosphatase was about 70%. The alkaline phosphatase complex was stable at 15℃ for 45 days.
     反应最适pH为7.2,不溶酶的相对活力为70%左右,以潮湿状态保存于15℃,45天内活力不变。
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     Immobilization technology for the enzyme electrode fabrication includes absorption,composite,crosslinking,covalently binding,entrapment methods and so on.
     对酶电极制备中酶的各种固定化方法的特点、优势进行了探讨 ;
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  相似匹配句对
     protein binding;
     蛋白结合;
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     On Binding And Layout of Periodicals
     新与美的交融——浅谈期刊的装帧设计
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     Immobilization technology for the enzyme electrode fabrication includes absorption,composite,crosslinking,covalently binding,entrapment methods and so on.
     对酶电极制备中酶的各种固定化方法的特点、优势进行了探讨 ;
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     Mechanistically, it covalently modified reduced cysteine62 of p50, thusblocking the binding of NF-κB oligonucleotide to nuclear proteins.
     分子机制研究结果表明,Andro 能够共价修饰处于还原状态的p50 蛋白上第62 位半胱氨酸,由于该氨基酸位于结合特定DNA 的关键部位,所以Andro 的共价结合阻断了NF-κB 二聚体与特定DNA 序列的结合。
短句来源
     protein was covalently linked with carbohydrate by O-glycosyllinkage.
     糖与蛋白的肽链之间的连接点类型是O-糖肽键。
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  covalently binding
Chromatographic studies were performed to measure myelin basic protein (MBP) interactions by covalently binding a number of different proteins to Sepharose and passing radioactive bovine MBP over these columns.
      
SUMO (small ubiquitin-related modifier) modulates protein structure and function by covalently binding to the lysine side chains of the target proteins.
      
A link between the FePc and the carbon was achieved by covalently binding imidazol to the carbon surface and then letting the FePc co-ordinate to the free nitrogen of the imidazol molecule.
      
An immobilized, biospecific ligand was prepared by covalently binding the cells to glutaraldehyde activated amino-Sepharose followed by stabilization of the cells with glutaraldehyde.
      
A novel magnetic nano-adsorbent was prepared by covalently binding polyacrylic acid (PAA) on Fe3O4 superparamagnetic nanoparticles (13.2 nm) via carbodiimide activation.
      
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Immobilized alkaline phosphatase has been prepared by using ABSE-cellulose as the basic material. The amino group of the ABSE-cellulose was first converted to the isothiocyano group by treatment with thiophosgene in dioxane and was then bound with alkaline phosphatase to obtain the immobilized enzyme. The optimum pH for covalently binding the enzyme was 7.2 in 0.1 M tris buffer. The relative activity of the immobilized alkaline phosphatase was about 70%. The alkaline phosphatase complex was stable at 15℃...

Immobilized alkaline phosphatase has been prepared by using ABSE-cellulose as the basic material. The amino group of the ABSE-cellulose was first converted to the isothiocyano group by treatment with thiophosgene in dioxane and was then bound with alkaline phosphatase to obtain the immobilized enzyme. The optimum pH for covalently binding the enzyme was 7.2 in 0.1 M tris buffer. The relative activity of the immobilized alkaline phosphatase was about 70%. The alkaline phosphatase complex was stable at 15℃ for 45 days. The amount of the alkaline phosphatase bound to the diazotized ABSE-cellulose and the relative activity of immobilized enzyme formed were almost identical with those bound to the p-isothiocyanobenzenesulphonylethyl cellulose. ABSE-Sephadex G-200 was converted to the isothiocyanate derivative in the same manner as the ABSE-cellulose and was used to bind the alkaline phosphatase.

以ABSE-纤维素为原料,用硫光气的二氧六环溶液处理,使氨基转变为异硫氰基,然后与碱性磷酸单酯酶键合。反应最适pH为7.2,不溶酶的相对活力为70%左右,以潮湿状态保存于15℃,45天内活力不变。与对-重氮基苯磺酰乙基纤维素为载体作比较,从键合酶量、相对活力来看均无显著区别。用葡聚糖凝胶G200作支持物,以相同的方法制成含异硫氰基的载体,与碱性磷酸单酯酶键合后,键合酶量和相对活力均与对-异硫氰基苯磺酰乙基纤维素-碱性磷酸单酯酶相同。

m-Carboxyphenyl-dimethylethylammonium iodide, an aoetylcholinesterase reversible inhibitor, was covalently bound to Sepharose-4B. After affinity chromatography with the above gel, the speoifio activity of the aoetylcholinesterase extracted from pig brain caudata nucleus by 0.5% sodium cholate increased 1024 folds, with 32% recovery of activity. The purified preparation shows a single precipitated line in immunodiffusion and shows four bands by enzyme staining in polyacrylamide gel eleotrophoresis. The...

m-Carboxyphenyl-dimethylethylammonium iodide, an aoetylcholinesterase reversible inhibitor, was covalently bound to Sepharose-4B. After affinity chromatography with the above gel, the speoifio activity of the aoetylcholinesterase extracted from pig brain caudata nucleus by 0.5% sodium cholate increased 1024 folds, with 32% recovery of activity. The purified preparation shows a single precipitated line in immunodiffusion and shows four bands by enzyme staining in polyacrylamide gel eleotrophoresis. The Miohaelis constant against acetylthiocholine is 8.9 ×10~(-5)M.

将乙酰胆硷酯酶的可逆抑制剂(间-羧苯二甲基碘乙基季铵盐)共价连接到Sepharose 4B上,用0.5%胆酸钠抽提的猪脑尾核乙酰胆硷酯酶经上述凝胶亲和层析,比活提高1024倍,得率为32%。纯化制剂的琼脂免疫双扩散呈单一的沉淀线,聚丙烯酰胺凝胶电泳分离出四条酶活力区带,对硫代乙酰胆硷的米氏常数为:8.9×10~(-5)M。

m-Carboxyphenyl-dimethylethylammonium iodide, an acetylcholinesterase rever-sible inhibitor, was covalently bound to Sepharose-4B. After affinity chromatographywith the above gel, the specific activity of the acetylcholinesterase extracted from pigbrain caudata nucleus by 0.5% sodium cholate increased 1024 folds, with 32% recoveryof activity. The purified preparation shows a single precipitated line in immuno-diffusion and shows four bands by enzyme staining in polyacrylamide gel electro-phoresis. The...

m-Carboxyphenyl-dimethylethylammonium iodide, an acetylcholinesterase rever-sible inhibitor, was covalently bound to Sepharose-4B. After affinity chromatographywith the above gel, the specific activity of the acetylcholinesterase extracted from pigbrain caudata nucleus by 0.5% sodium cholate increased 1024 folds, with 32% recoveryof activity. The purified preparation shows a single precipitated line in immuno-diffusion and shows four bands by enzyme staining in polyacrylamide gel electro-phoresis. The Michaelis constant against acetylthiocholine is 8.9×10~(-5)M.

将乙酰胆硷酯酶的可逆抑制剂(间-羧苯二甲基碘乙基季铵盐)共价连接到Sepharose 4B上,用0.5%胆酸钠抽提的猪脑尾核乙酰胆硷酯酶经上述凝胶亲和层析,比活提高1024倍,得率为32%。纯化制剂的琼脂免疫双扩散呈单一的沉淀线,聚丙烯酰胺凝胶电泳分离出四条酶活力区带,对硫代乙酰胆硷的米氏常数为:8.9×10~(-5)M。

 
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