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binary expression
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  双元表达
     Construction of the binary expression vector proved correct,when it was checked by PCR and the triparental mating Agrobacterium Tumefaciens was selected by culture medium containing 50 mg/L Kanamycin,25 mg/L Streptomycin and 50 mg/L Rifampicin.
     在含50mg/L卡那霉素、25 mg/L链霉素和50 mg/L利福平的YEB培养基上筛选及对融合农杆菌质粒进行P1基因的PCR检测,证明植物双元表达载体pB inFMDV-P1构建正确。
短句来源
     Construction of a Plant Binary Expression Vector with Pti5-VP16 Gene and Studies on Transgenic Tobacco Plant
     Pti5-VP16基因植物双元表达载体的构建及其转化烟草的研究
短句来源
     Construction of the binary expression vector of transgenic plant with foot and mouth disease virus, Asia-1 type, YNBS/58 strain
     口蹄疫病毒亚洲I型YNBS/58株P12A-3C转基因植物双元表达载体的构建
短句来源
     1) Three binary expression vectors were constructed: 1) p13W4H, containing loxcDNA hairpin structure controlled by endosperm specific promoter (ESP) and hpt gene;
     1) 构建了三个用于转化的双元表达载体:A:p13W4H,含胚乳特异表达启动子、lox cDNA发夹结构及潮霉素选择标记基因hpt;
短句来源
     Cloning of foot-and-mouth disease virus structural gene P1 and construction of binary expression vector in plants
     FMDV结构基因P1的克隆与植物双元表达载体的构建
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  “binary expression”译为未确定词的双语例句
     Three plant binary expression vectors pNAR501,pNAR502 and pNAR503 were constructed which carried exon 2-exon 3,5′partial deletion exon 1 and 5′partial deletion exon 1-exon 2-exon 3 of Pib gene driven by 35S separately.
     将Pib结构基因克隆到双元载体,构建了3个植物表达载体pNAR501、pNAR502和pNAR503,这3个载体分别携带由35S驱动Pib结构基因的外显子2外显子3、5′端部分缺失外显子1和5′端部分缺失的外显子1外显子2外显子3的不同片段。
短句来源
     Construction and Transformation of the Antisense pBI121-NR-LEACS2 Binary Expression Vector into Tomato
     反义pBI121-NR-LEACS2双价表达载体的构建及其向番茄的转化
短句来源
     The plant binary expression vector of K88-ST was transformed into A grobacterium EHA 105. It provided a reliable basis for the further study on K88-ST.
     并将其转化农杆菌EHA105,为K88-ST基因的进一步研究奠定基础。
短句来源
     2.Insert the above DNA sequence into the pBI121 with CaMV35S and constructed the soybean binary expression vector pBI 121-IN.
     2.将人胰岛素A、B链基因与含有超表达组成型启动子—CaMV35S启动子的质粒载体pBI121重组构建成植物表达载体pBI121-IN。
短句来源
     Plant binary expression vector pLBI containing lysine-rich protein gene, 35S promoter, NOS terminator and NPTII gene was constructed.
     构建了植物表达载体pLBI,该载体携带 35S启动子、高赖氨酸蛋白基因 (LRP)、NPTII基因和NOS终止子 .
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  相似匹配句对
     The expression
     在交界瘤、良性瘤中无表达。
短句来源
     Expression of E.
     将阳性重组质粒转化表达受体菌E.
短句来源
     The binary expression vector was constructed by triparental mating.
     采用三亲融合法构建植物双元表达载体pB inFMDV-P1。
短句来源
     Binary Optics
     二元光学
短句来源
     Optimization of the Retrieval Algorithms by Improving Binary Tree of Query Expression
     信息检索中改进二叉树优化检索算法
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  binary expression
We constructed a binary expression vector with the Cre/lox system with a view to eliminating a marker gene from transgenic plants conveniently.
      
The coat protein (CP) gene of the potato virus Y (PVY) strain N605 has been cloned into a plant binary expression vector and introduced into the potato variety Bintje.
      
A novel binary expression vector for production of human IL-10 in Escherichia coli and Bifidobacterium longum
      
The sense and antisense 260-bp fragments were amplified and subcloned into pFGC1008 binary expression vectors.
      
To examine the function of Drosophila Rad51 (DmRad51) in cell cycle regulation and apoptosis, DmRad51 protein was overexpressed using a heat shock-inducible promoter or the UAS-GAL4 binary expression system.
      
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SacB gene from Bacillus subtilis,which encodes levansucrase,was cloned by the PCR method and then coupled to the carboxypeptidase Y vacuolar sorting signal (cpy) from Saccharomyces cerevisiach.The chimeric gene was sequenced and inserted into the plant binary expression vector (pBin438) containing NPT Ⅱ gene,then transferred into tobacco (Nicotiana tabacum var.K326) by agrobacterium mediated transformation.Following selection by kanamycin,some transformed shoots were normally rooted on MS medium...

SacB gene from Bacillus subtilis,which encodes levansucrase,was cloned by the PCR method and then coupled to the carboxypeptidase Y vacuolar sorting signal (cpy) from Saccharomyces cerevisiach.The chimeric gene was sequenced and inserted into the plant binary expression vector (pBin438) containing NPT Ⅱ gene,then transferred into tobacco (Nicotiana tabacum var.K326) by agrobacterium mediated transformation.Following selection by kanamycin,some transformed shoots were normally rooted on MS medium supplemented 1%NaCl,but the controls were not.The plantets were transferred to pots containing vermiculite and watered with hoagland’s nutrient solution added 1% NaCl and showed less growth inhibitation than controls.By PCR and Northern analysis,it was verified that the SacB gene had been integrated and transcripted in those transgenic plants.The results indicated that the SacB gene could enhance salt tolerance of transgenic plant.

采用PCR方法克隆了枯草杆菌(Bacilussubtilis)果聚糖蔗糖转移酶基因(SacB),将其与克隆自酵母(Saccharomycescerevisiae)的羧肽酶A的液泡引导信号序列连接得到嵌合基因。测序验证后,插入含NPTⅡ基因的植物双元表达载体pBin438中,经农杆菌介导转化烟草。部分经卡那霉素筛选的抗性芽能在含1%NaCl的MS培养基上正常生根,而未转化芽不能生根或根生长缓慢。转基因小苗移入盛蛭石的花盆并浇灌含1%NaCl的hoagland′s营养液,17d后,其中一些转基因烟草植株生长良好,而未转化苗出现明显萎蔫。PCR扩增及Northern分析证实SacB基因已导入转基因植株并得到转录。此结果表明SacB基因的植物基因工程可提高烟草植株的耐盐性。

Plant binary expression vector of human Interferon-γ(HIFN-γ) was constructed to study the expression of in medicinal plant. The method was as follow. The plasmid of P SWIF including interferon-γ gene was digested with pst Ⅰ and low melting point agarose was used to recover the fragment of HIFN-γ.Then the recovered fragment was cloned to the vector of P Bluescript SK(+) to form an intermediate vector of P SKIFN .The P SKIFN was digested with kpn I /xba Ⅰ and then coned to plant...

Plant binary expression vector of human Interferon-γ(HIFN-γ) was constructed to study the expression of in medicinal plant. The method was as follow. The plasmid of P SWIF including interferon-γ gene was digested with pst Ⅰ and low melting point agarose was used to recover the fragment of HIFN-γ.Then the recovered fragment was cloned to the vector of P Bluescript SK(+) to form an intermediate vector of P SKIFN .The P SKIFN was digested with kpn I /xba Ⅰ and then coned to plant expression vector of P ROK Ⅱ to form the plant binary expression vector of P ROKIFN . The P ROKIFN was identified with digestion of kpn Ⅰ / xba Ⅰ and automatic sequence system. The result of restriction analysis showed that the length of inserted fragment was about 1.3 kb and that of automatic sequence showed the sequence of inserted fragment was correct.

为研究在药用植物中表达人干扰素 (IFN) ,构建了人IFN -γ植物双元表达载体。方法是将IFN -γ基因的pstI片段插入克隆载体PBluescriptSK(+) 中 ,通过蓝白斑筛选以xbaI kpnI证实 ,以ndeI kpnI鉴定插入方向。得到中间载体PSKIFN,用xbaI kpnI双酶切PSKIFN ,回收 1.3kb片段 ,将此片段插入植物表达载体PROKII上 ,经限制性酶切分析及自动测序 ,结果证实插入片段大小及序列正确 ,构建获得成功 ,为进一步在药用植物中表达IFN -γ奠定了基础

By Agrobacterium-mediated method, the Sinningsa speciosa was transformed with binary expression vector pBIN m-gfp-ER,containing NPT gene and GFP gene. Obtained some Kanamycin(Kan)-resistant regeneratied green shoots, PCR test showed that three green shoots can amplified bands with GFP primers. Also got postive siginal in the dot blot hybridizition analysis with these green shoots. These results indicate that the foreign gene had transformed into plants genome. Observing these transgenic plants with fluorescent...

By Agrobacterium-mediated method, the Sinningsa speciosa was transformed with binary expression vector pBIN m-gfp-ER,containing NPT gene and GFP gene. Obtained some Kanamycin(Kan)-resistant regeneratied green shoots, PCR test showed that three green shoots can amplified bands with GFP primers. Also got postive siginal in the dot blot hybridizition analysis with these green shoots. These results indicate that the foreign gene had transformed into plants genome. Observing these transgenic plants with fluorescent microscope, it was found that green fluorescent can be seen in part cells of leaves and bloom.

利用农杆菌介导法 ,用含有绿色荧光蛋白基因的二元双价表达载体pBINm -gfp5 -ER转化大岩桐 ,并得到卡那霉素 (Kanamycin ,Kan)抗性再生植株 .对其进行初步PCR检测 ,结果表明 ,K2 0 0 (含Kan 2 0 0mg/L)培养基上的绿苗中有 3株PCR结果呈阳性 .对PCR阳性的植株进行了点杂交分析 ,均表现出较强的杂交信号 ,这说明外源基因已整合转入到大岩桐基因组中 .在荧光显微镜下观察转基因大岩桐 ,发现部分花、叶细胞均发出一定强度的绿色荧光

 
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