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植酸酶酶活
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  phytase activity
     Phytase producing Aspergillus Niger spore and protoplast were treated by 20W or 15W power high repetition rate, 1.06 um, 15KHz soundoptic Qmodulation Nd:YAG laser at different time, regenerated fungus was counted and the phytase activity of mutated fungus were measured after priscreen and rescreen.
     用1.06um,15KHz高重复率声光调QNd:YAG激光以20W和15W的辐照功率分别照射产植酸酶的黑曲霉孢子液和原生质体液不同时间,检定再生菌落数并经透明圈初筛和摇瓶复筛,测定诱变菌株所产植酸酶酶活
短句来源
     High active phytase producing fungs-Aspergillus niger were selected by mutiple UV mutation, the definitions of phytase activity were analysised and the measure wavelength of the enzyme was modified, the factors that influence the preparation of protoplast were investigated.
     本文以多轮紫外诱变为主线技术筛选植酸酶的黑曲霉高产菌,分析比较植酸酶酶活定义和植酸酶酶活测定方法并修正其测量波长,考查黑曲霉原生质体制备的影响因素,并在此基础上,用原生质体紫外复合诱变和原生质体融合技术筛选植酸酶的黑曲霉产生菌。
短句来源
     Nine strains A. Nigers which were preserved in lab and purchased were determined phytase activity, and screened out a high productioon strain-3928, phytase activity could get to 693μg/(g·min).
     对实验室保存和购买的 9 株黑曲霉菌株进行了产植酸酶酶活测定,筛选出一株产植酸酶较高的菌株 3928,酶活达 693 μg/(g·min)。
短句来源
     However,the ANNPRCTRL strategy,pH-Stat method,and others could not effectively increase the phytase activity.
     然而,在诱导期ANNPR-Ctrl和pH-Stat法等均不能有效地提高植酸酶酶活
短句来源
     A mutant strain was screened by phytase activity observation, which increased about 2.2 times of the original strain.
     考查突变菌种的植酸酶酶活 ,筛选到其中一个变异株比原始黑曲霉 (II)亲本株的植酸酶酶活提高了 2 .2倍。
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  “植酸酶酶活”译为未确定词的双语例句
     In this study the definition is under the conditions of 55,pH4.6,from 0.1 % phytic acid sodium salt,release 1 nmol free phosphorus in one mintiue is one enzyme activity unit and the acetone method is the suitable measure way of the research was also decided.
     确定本实验用的植酸酶酶活定义为:55℃,pH4.6的条件下,每分钟从0.1%的植酸钠溶液中释放1nmol的无机磷为一个酶活单位(u)。
短句来源
     Under the optimal conditions, phytase was accumulated and its activity could reach 2.63×10~5u/ml, which was 5.52 times as high as that of before optimizing and increased by 9.11 times than the activity of KM71 2.6×10~4u/ml reported by Yanming Han.
     发酵结束时植酸酶酶活可达2.63×10~5u/ml,是培养条件优化前酶活力的5.52倍,比国外Yanming Han报道的KM71重组子的酶活2.6×10~4u/ml提高了9.11倍。
短句来源
     niger NRRL3135 (GenBank Accession:M94550),the most highly secreting\|phytase strain,shows that the nucleotide homology is as high as 96.746%,and the amino acid homology comes up to 97.64%. The phyA of A.
     黑曲霉N2 5与产植酸酶酶活最高的天然黑曲霉标准菌株NRRL31 35的植酸酶phyA基因 (GenBankAccession :M94550 )相比较 ,其同源性为 96 746% ,编码的氨基酸序列同源性为 97 64%。
短句来源
     The lyse of the transfected DEF was used to test the activity of phytase by the ammonium molybdic method and it reached to 977 U/mL.
     钼酸胺 法测定试验样品植酸酶酶活为977 U/mL。
短句来源
     The measure wavelength was modified to 420 nm as preciseA protoplasting technique has been developed for the Aspergillus niger.
     确定本实验用的植酸酶酶活测定方法为丙酮法,测量波长修正为420nm。
短句来源
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  相似匹配句对
     semitecum was capable to produce some higher Cellulase activity than R.
     stolonifer产的高。
短句来源
     Methods SOD, POD and CAT enzymes analysis.
     方法 分析技术。
短句来源
     Effect of Supplemental Vegetable Phytase on Grown Performance and the Phytase Activity in Chyme of Chickens
     植物性植酸对蛋雏鸡生长性能的影响及体内研究
短句来源
     Production of phytase by ferment of Aspergillus ficuum and kinetic characterization of phytase
     无花果曲霉植酸发酵及动力学
短句来源
     Analysis of the Bacterial Strain Enzyme Activity Produced from the Phytase.
     植酸产生菌株的力研究
短句来源
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  phytase activity
A maximum phytase activity (468.22 U/mL) was obtained using this production medium containing 2% (v/v) Tween-20 after 72 h of fermentation at 45°C in shake-flask cultures with a rotation of 150 rpm.
      
The phytase enzyme was found to be thermostable, and the optimal temperature for phytase activity was found to be 55°C.
      
Under the optimal conditions, over 557.9?mU/ml of phytase activity was produced within 72?h of fermentation at the shake flask level.
      
This is a very high level of phytase activity produced by yeasts.
      
Phytase activity was found in fermented soybean meal, and this activity may degrade added phytate in fermented soybean meal-based diet.
      
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In this experiment,the usual ALKO Method was adopted to determine the phytase activities in sixteen kinds of plant feedstuffs most in use.The phytase aetivities, under the condition of pH 5.0, 37℃ and in 15 minutes reactiontime,were defined as the nmol amounts of inorganic phosphate which were let free from sodium phytate per minute pet gram.The results showed that the phytase activity(PU/g) ranged from 1,400 to 1,800 in wheat middlings and shorts and wheat bran; from 700 t0 800 in barely and wheat; from...

In this experiment,the usual ALKO Method was adopted to determine the phytase activities in sixteen kinds of plant feedstuffs most in use.The phytase aetivities, under the condition of pH 5.0, 37℃ and in 15 minutes reactiontime,were defined as the nmol amounts of inorganic phosphate which were let free from sodium phytate per minute pet gram.The results showed that the phytase activity(PU/g) ranged from 1,400 to 1,800 in wheat middlings and shorts and wheat bran; from 700 t0 800 in barely and wheat; from 150 to 180 in soybean,soybean meal and rapeseed meal; from 100 to 200 in feedstaffs such as corn and it's byproducts.

本试验采用通用的ALKO法测定了16种常用植物性饲料中植酸酶酶活。结果表明:次粉、麦麸酶活为最高,为1400-1800PU/g;小麦、大麦为700-800PU/g;大豆、豆饼、菜籽饼为150-180PU/g;玉米及其副产物为100-200PU/g。

The phyA encoding phytase of Aspergillus niger N25 was amplified by the polymerase chain reaction (PCR) with primers designed according to the sequences of the phyA in GenBank.The amplified fragment was cloned and sequenced.The results show that:the coding region is 1506bp in size,includes a 102bp intron,and encodes a peptide of 476 amino acid residues,in which there is a signal peptide with 19 amino acids and a mature peptide of 448 amino acids.Comparison of this sequence with the phyA of the...

The phyA encoding phytase of Aspergillus niger N25 was amplified by the polymerase chain reaction (PCR) with primers designed according to the sequences of the phyA in GenBank.The amplified fragment was cloned and sequenced.The results show that:the coding region is 1506bp in size,includes a 102bp intron,and encodes a peptide of 476 amino acid residues,in which there is a signal peptide with 19 amino acids and a mature peptide of 448 amino acids.Comparison of this sequence with the phyA of the natural A.niger NRRL3135 (GenBank Accession:M94550),the most highly secreting\|phytase strain,shows that the nucleotide homology is as high as 96.746%,and the amino acid homology comes up to 97.64%.The phyA of A.niger N25 strain in this paper is appropriate to be used to construct the phytase gene\|engineering bacteria.The phyA and its amino acid sequence have been accessed by GenBank (Accession:AF218813,AAF25481.1).

通过对黑曲霉N2 5植酸酶phyA基因PCR扩增 ,获得了一条长约 1 6kb的特异性PCR产物 ,并进行了酶切鉴定。然后在pUC1 8质粒中构建了含有目的基因片段的克隆质粒pFNP 1。DNA序列测定表明 ,目的基因片段含有植酸酶phyA基因的完整序列 ,phyA基因全长1 50 6bp,其中包含一段长 1 0 2bp的内含子 ,编码 467个氨基酸 ,5’端有一段编码 1 9个氨基酸的信号肽序列。黑曲霉N2 5与产植酸酶酶活最高的天然黑曲霉标准菌株NRRL31 35的植酸酶phyA基因 (GenBankAccession :M94550 )相比较 ,其同源性为 96 746% ,编码的氨基酸序列同源性为 97 64%。将黑曲霉N2 5植酸酶phyA基因序列及其相应的氨基酸序列在国际基因库中注册 (注册号分别为 :AF2 1 881 3,AAF2 5481 1 ) ,此基因是目前中国在国际基因库中注册的第一个植酸酶phyA基因。

Phytase producing Aspergillus Niger spore and protoplast were treated by 20W or 15W power high repetition rate, 1.06 um, 15KHz soundoptic Qmodulation Nd:YAG laser at different time, regenerated fungus was counted and the phytase activity of mutated fungus were measured after priscreen and rescreen. It showed that laser with power 20W acted on spore more than 1 min, the lethal ratio is almost 100%; laser with power 15W acted on protoplast 20s~60s, the survival ratio among 8.571%~117.14%, the forward mutate...

Phytase producing Aspergillus Niger spore and protoplast were treated by 20W or 15W power high repetition rate, 1.06 um, 15KHz soundoptic Qmodulation Nd:YAG laser at different time, regenerated fungus was counted and the phytase activity of mutated fungus were measured after priscreen and rescreen. It showed that laser with power 20W acted on spore more than 1 min, the lethal ratio is almost 100%; laser with power 15W acted on protoplast 20s~60s, the survival ratio among 8.571%~117.14%, the forward mutate ratio among 27.45%~100%, the forward mutate range is 60.69%~124.96%, the result show that protoplast of Aspergillus Niger has more durability against laser and gets better mutable effect than that of the spore. One most high phytase activity mutation fungus was screened, its phytase activity was promoted 3.75.

用1.06um,15KHz高重复率声光调QNd:YAG激光以20W和15W的辐照功率分别照射产植酸酶的黑曲霉孢子液和原生质体液不同时间,检定再生菌落数并经透明圈初筛和摇瓶复筛,测定诱变菌株所产植酸酶酶活。结果表明:20W的功率辐照黑曲霉孢子1 以上,致死率近乎100%,15W功率辐照原生质体液20"~4 ,存活率在8.571%~117.14%之间,正变率在27.45%~100%之间,正变辐度60.69%~124.96%,说明原生质体比孢子耐受激光诱变的能力强,并且诱变效果好。筛选到了一株酶活提高达3.75倍的单个变异菌株。

 
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