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人膀胱癌    
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  human bladder
    Interleukin-6 Production by Human Bladder Cell Line (T24)
    人膀胱癌T_(24)细胞系产生IL-6的条件探讨
短句来源
    Expression and Regulation of some Oncogenes in Human Bladder Cancer
    人膀胱癌中某些癌基因的研究
短句来源
    Retrovirus mediated human IL 2 gene transfer in human bladder carcinoma cells
    逆转录病毒介导人白细胞介素2基因在人膀胱癌细胞中的表达
短句来源
    PREPARATION OF THE PHAGE SINGLE CHAIN ANTIBODY OF ANTI HUMAN BLADDER CANCER
    抗人膀胱癌噬菌体单链抗体的初步制备
短句来源
    Expression and Function of TNF α cDNA mutant in human bladder cancer EJ cell lines
    TNF-α cDNA突变体在人膀胱癌细胞株EJ中的表达及作用
短句来源
更多       
  human bladder cancer
    Expression and Regulation of some Oncogenes in Human Bladder Cancer
    人膀胱癌中某些癌基因的研究
短句来源
    PREPARATION OF THE PHAGE SINGLE CHAIN ANTIBODY OF ANTI HUMAN BLADDER CANCER
    抗人膀胱癌噬菌体单链抗体的初步制备
短句来源
    Expression and Function of TNF α cDNA mutant in human bladder cancer EJ cell lines
    TNF-α cDNA突变体在人膀胱癌细胞株EJ中的表达及作用
短句来源
    This laid the foundation to develop hIL 2 gene transducted tumor vaccine of human bladder cancer.
    转hIL-2基因在人膀胱癌细胞中的高效表达,为人膀胱癌转hIL-2基因瘤苗的应用研究打下了基础。
短句来源
    Methods A TNF α cDNA mutant was selected as the target gene. The novel mutant was transduced into human bladder cancer EJ cell lines with the plasmid pcDNA 3.1(+) as the vector by the method of calcium phosphate DNA coprecipitation.
    方法以突变的TNFαcDNA为目的基因,pcDNA3.1(+)为载体,用磷酸钙沉淀法将其导入人膀胱癌细胞株EJ。
短句来源
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  human bladder carcinoma
    Retrovirus mediated human IL 2 gene transfer in human bladder carcinoma cells
    逆转录病毒介导人白细胞介素2基因在人膀胱癌细胞中的表达
短句来源
    The results showed that the anti mouse MFH 1 monoclonal antibody was able to identify specifically the mouse MFH 1 protein which was expressed in human bladder carcinoma HTB 9 cell transfected with CX MFH 1 plasmid by Western blot analysis. The myoblasts C2C12 could express the endogenous MFH 1 protein in its nucleus.
    小鼠肌胚细胞C2C12低水平地表达内源性MFH 1蛋白以及导入小鼠MFH 1cDNA的人膀胱癌细胞HTB9也表达小鼠MFH 1蛋白 ,这种蛋白质定位于细胞核中 .
短句来源
    Preparation and identification of bifunctional antibodies against human bladder carcinoma
    抗人膀胱癌双功能抗体的制备与鉴定
短句来源
    The cDNA for human G-CSF was cloned from total RNA of human bladder carcinoma 5637 cells following PCR amplification. Sequencing data proved that the cloned cDNA contained the intact coding region of human G-CSF gene which was 612 bp in length, encoding 30 amino acids of signal peptide and 174 amino acids of the mature protein.
    利用多聚酶链反应技术,从人膀胱癌细胞系5637细胞中快速扩增并克隆了人粒细胞集落刺激因子cDNA,序列分析证明,该cDNA包含人粒细胞集落刺激因子的全部编码基因,全长612bp,编码30个氨基酸的信号肽和174个氨基酸的成熟蛋白。
短句来源
    By using Liposollleunlediated transfection, eukaryotic expression vector tgCMV/hytk-IRES-gfP was transfected into COS7 cell and human bladder carcinoma cells EJ. The results of PCR and microscopy detection show that the hytk-IRES-GFP gene was successfully transferred into COS7 cell and EJ cells. There were no differences in the growth pattern or the morphology between EJ and EJ/hytk-IRES-GFP cells.
    利用脑炎心肌炎病毒 (encephalomyocarditis virus, EMCV)的内部核糖体进入位点( internal ribosome entry site, IRES),将 gfp 的cDNA与hytk真核表达载体重组,构建获得含gfp和hytk基因的重组质粒:Lipofectin介导下分别转染 COS-7细胞及人膀胱癌细胞株EJ,并检测其表达情况。
短句来源
  human bladder tumor
    AIM: To increase the expressed level of a human-mouse chimeric antibody against human bladder tumor in dihydrofo-late reductase (DHFR) defective CHO cells(CHO/DHFR) via weakening the transcription of DHFR gene in the vector.
    目的:提高抗人膀胱癌人-鼠嵌合抗体在二氢叶酸还原酶缺陷的CHO细胞株(CHO/DHFR-)中表达的产量。
短句来源
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  human bladder
TBARS, Carnitine, and Reduced Glutathione Levels in Human Bladder Carcinoma
      
Select cytocompatibility experiments (specifically adhesion and long-term growth studies) were performed on these scaffolds using human bladder smooth muscle cells (BdSMCs).
      
Construction and expression of a human-mouse chimeric antibody against human bladder cancer
      
Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer.
      
Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR.
      
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  human bladder cancer
Construction and expression of a human-mouse chimeric antibody against human bladder cancer
      
Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer.
      
Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR.
      
Conclusion: The constructed chimeric antibody was expressed successfully in eukaryotic cells, and the chimeric antibody had desired affinity against human bladder cancer cells.
      
The effects of EPI-CDMN associated with external pulsed electromagnetic fields (PEMFs) (10 mT) on killing human bladder cancer BIU-87 cells were studied by MTT assay and Annexin-V/PI double-labeled flow cytometry technique, respectively.
      
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  human bladder carcinoma
TBARS, Carnitine, and Reduced Glutathione Levels in Human Bladder Carcinoma
      
Expression of a mutant hTERT in human bladder carcinoma cell line T24 and its clinical significance
      
Apoptosis induced by ginsenoside Rg3 in a human bladder carcinoma cell line
      
Plasmids encoding each of the mutated SF-1 proteins were cotransfected with the StAR and HDL-R promoter constructs into human bladder carcinoma (HTB-9) cells in the presence or absence of dibutyryl cAMP.
      
Erythroid progenitor cells (BFUe: burst forming units-erythroid) are cultured in 1 ml of 0.35% agarose in IMDM with 30% FBS and conditioned medium from human bladder carcinoma cell line 5637 or recombinant interleukin 3.
      
更多          
  human bladder tumor
An immunoassay using a human bladder tumor tissue culture cell line (J-82) as antigen and a modified avidin-biotin-complex (ABC) method were used to examine human serum samples.
      
Cytotoxic effects of alpha- and gamma-interferon and tumor necrosis factor in human bladder tumor cell lines
      
We investigated the activity of alpha-interferon (α-IFN), gamma-interferon (γ-IFN) and tumor necrosis factor-alpha (TNF-α) in a panel of ten human bladder tumor cell lines.
      
We conclude that α-IFN and TNF are active as single agents and synergistic when used together in vitro in human bladder tumor cell lines.
      
This model more closely resembled the characteristics of human bladder tumor when compared to other bladder cancer models.
      
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A mutant of thermophilic Mastigocladus laminosus, a blue-green algae, has been obtained by mutantion of EMS ( Ethyl Methanesulfonate, Sigma ) and selection of growth on 9T24C tumor cells.Extracts of mutant promote the growth of 9T24C cells in DME/F12 medium supplemented with 0.3% FBS (Fetal Bovine Serum) . The cell number of 9T24C cells increased 3.1-folds in six days when 100 fig/ml extract was added into medium. The molecular weight of unknown active component more than 3500.

本文以EMS(乙基甲烷磺酸盐,Sigma)诱变嗜温的层理鞭枝藻(Mastigocladus laminosus),并经9T24C人膀胱癌细胞生长选择获得突变体。在0.3%FBS(胎牛血清)+DME/F12培养液中加入100μg/ml层理鞭枝藻突变体提取液可促进9T24C癌细胞数在6天中增长3.1倍,而在同样条件下野生型层理鞭枝藻的提取液则对9T24C癌细胞无明显刺激生长作用。这种未知的刺激癌细胞生长的活性物质分子量>3500。

The optimal culture condition (initial concentration, passage interval, cryostorage, etc.) of human bladder cell line (T24) was studied, on which the spontaneous secretion of high value of interleukin-6 (IL-6) by T24 cells was observed. LPS, PMA, PJV and BCG promoted the IL-6 production by 9.03, 4.69, 2.00, 1.33 folds respectively. The 72.11 folds of IL-6 production was obtained by using superinduction method with PMA, cyclohexmide and actinomycin-D.

利用IL-6分泌细胞株(人膀胱癌T_(24)细胞),建立了最佳天然IL-6产生条件和IL-6超诱生方法,IL-6分泌量最大可达15 576±1042.3μ/ml。同时对T_(24)细胞连续培养和冻存后IL-6产生水平进行了观察,表明该细胞分泌IL-6的能力十分稳定。

Using Northern and dot blot analysis, we investigated the expression of c-myc, c-fos abd erbB and regulation of oncogenes by TPA in BIU-87 cells a human bladder transitional cell carcinoma line We found expression of these oncogenes and induc-tion of these genes by TPA.We also found high expression of c-myc, c-fos, erbB, N-ras in human bladder cancer tissues.In contrast, only low level expression of N-ras was detected in normal bladder tissues.These data suggest that the expression of some oncogenes can be modulated...

Using Northern and dot blot analysis, we investigated the expression of c-myc, c-fos abd erbB and regulation of oncogenes by TPA in BIU-87 cells a human bladder transitional cell carcinoma line We found expression of these oncogenes and induc-tion of these genes by TPA.We also found high expression of c-myc, c-fos, erbB, N-ras in human bladder cancer tissues.In contrast, only low level expression of N-ras was detected in normal bladder tissues.These data suggest that the expression of some oncogenes can be modulated in a protein kinase C dependent manner and high level expression of some oncogenes are responsible for the abnormal growth in bladder carcinoma.

本实验应用Northern和斑点印渍杂交技术探测了人膀胱癌细胞株中c-myc、c-fos、erbB等癌基因的表达,以及TPA对这些癌基因表达的调控,发现BIU-87细胞有这些癌基因的表达,并能被TPA所增强,同时也发现人膀胱癌组织有c-myc、c-fos、erbB、N-ras基因的高表达。提示蛋白激酶C的激活可以诱导某些癌基因的表达。多种癌基因的表达异常可能在膀胱癌中起重要作用。

 
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