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人膀胱癌    
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  human bladder
    After treatment with these two prodrugs for 6~24 h,the growth inhibition rates on human bladder cancer EJ cells and renal tubular epithelial(HKC) cells were detected using MTT colorimetry.
    MTT比色分析法比较两种姜黄素前体药物作用6~24 h后,人膀胱癌EJ细胞及肾小管上皮HKC细胞生长抑制的差异。
短句来源
  human bladder cancer
    After treatment with these two prodrugs for 6~24 h,the growth inhibition rates on human bladder cancer EJ cells and renal tubular epithelial(HKC) cells were detected using MTT colorimetry.
    MTT比色分析法比较两种姜黄素前体药物作用6~24 h后,人膀胱癌EJ细胞及肾小管上皮HKC细胞生长抑制的差异。
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  human bladder
TBARS, Carnitine, and Reduced Glutathione Levels in Human Bladder Carcinoma
      
Select cytocompatibility experiments (specifically adhesion and long-term growth studies) were performed on these scaffolds using human bladder smooth muscle cells (BdSMCs).
      
Construction and expression of a human-mouse chimeric antibody against human bladder cancer
      
Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer.
      
Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR.
      
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  human bladder cancer
Construction and expression of a human-mouse chimeric antibody against human bladder cancer
      
Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer.
      
Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR.
      
Conclusion: The constructed chimeric antibody was expressed successfully in eukaryotic cells, and the chimeric antibody had desired affinity against human bladder cancer cells.
      
The effects of EPI-CDMN associated with external pulsed electromagnetic fields (PEMFs) (10 mT) on killing human bladder cancer BIU-87 cells were studied by MTT assay and Annexin-V/PI double-labeled flow cytometry technique, respectively.
      
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  human bladder tumor
An immunoassay using a human bladder tumor tissue culture cell line (J-82) as antigen and a modified avidin-biotin-complex (ABC) method were used to examine human serum samples.
      
Cytotoxic effects of alpha- and gamma-interferon and tumor necrosis factor in human bladder tumor cell lines
      
We investigated the activity of alpha-interferon (α-IFN), gamma-interferon (γ-IFN) and tumor necrosis factor-alpha (TNF-α) in a panel of ten human bladder tumor cell lines.
      
We conclude that α-IFN and TNF are active as single agents and synergistic when used together in vitro in human bladder tumor cell lines.
      
This model more closely resembled the characteristics of human bladder tumor when compared to other bladder cancer models.
      
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  human bladder tumor
An immunoassay using a human bladder tumor tissue culture cell line (J-82) as antigen and a modified avidin-biotin-complex (ABC) method were used to examine human serum samples.
      
Cytotoxic effects of alpha- and gamma-interferon and tumor necrosis factor in human bladder tumor cell lines
      
We investigated the activity of alpha-interferon (α-IFN), gamma-interferon (γ-IFN) and tumor necrosis factor-alpha (TNF-α) in a panel of ten human bladder tumor cell lines.
      
We conclude that α-IFN and TNF are active as single agents and synergistic when used together in vitro in human bladder tumor cell lines.
      
This model more closely resembled the characteristics of human bladder tumor when compared to other bladder cancer models.
      
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The antitumor effects of Chinese herbs “Tian Bao Zhong Ning San” were studied on cells of fibroblast lines of mouse (3T3), bladder carcinoma cell lines (EJ), throat carcinoma cell lines(IIep 2), stomach carcinoma cell lines(MGC 803), hepatic carcinoma cell lines (Q3) and lung carcinoma cell lines (Spc A 1) with cultured cells in vivo. And the decreasing toxicity effects of the prescription with normal mice and cyclophosphamide (CP) treated mice were also studied. The results showed that “Tian Bao Zhong Ning...

The antitumor effects of Chinese herbs “Tian Bao Zhong Ning San” were studied on cells of fibroblast lines of mouse (3T3), bladder carcinoma cell lines (EJ), throat carcinoma cell lines(IIep 2), stomach carcinoma cell lines(MGC 803), hepatic carcinoma cell lines (Q3) and lung carcinoma cell lines (Spc A 1) with cultured cells in vivo. And the decreasing toxicity effects of the prescription with normal mice and cyclophosphamide (CP) treated mice were also studied. The results showed that “Tian Bao Zhong Ning San” have the effets of antitumor, and cold decrease the toxicity of CP.

采用体外细胞培养法观察“天宝肿宁散”复方对人膀胱癌细胞(EJ)、人喉癌细胞(Hep2)、人低分化粘液腺胃癌细胞(MGC803)、人肝癌细胞(Q3)和人肺癌细胞(SpcA1)等肿癌细胞及小鼠成纤维细胞(3T3)的生物学作用,并观察该方剂对正常小鼠和经环磷酰胺(CP)处理小鼠的免疫机能、外周血象、造血功能及生存质量的影响。其结果显示该方剂具有一定杀伤肿瘤细胞的作用,并对环磷酰胺(CP)的毒副作用具有减毒效应。

Objective:To explore the biological effects of Tian Bao Zhong Ning San on different cell lines. Methods:Cell culture in vitro was used to investigate the effects of Chinese medicinal heabs Tian Bao Zhong Ning San on mouse fibroblast,bladder carcinoma cell lines (EJ),throat carcinoma cell lines (Hep2),stomach carcinoma cell lines (MGC803),hepatic carcinoma cell lines (Q3) and lung carcinoma cell lines (SpcA1). Results:The prescription proved to be...

Objective:To explore the biological effects of Tian Bao Zhong Ning San on different cell lines. Methods:Cell culture in vitro was used to investigate the effects of Chinese medicinal heabs Tian Bao Zhong Ning San on mouse fibroblast,bladder carcinoma cell lines (EJ),throat carcinoma cell lines (Hep2),stomach carcinoma cell lines (MGC803),hepatic carcinoma cell lines (Q3) and lung carcinoma cell lines (SpcA1). Results:The prescription proved to be effective in inhibiting the survival and protein metabolism of the cancer cells,inducing the morphalogical changes of the cultured cells. But no such effect was observed on mouse cell 3T3,suggesting that the prescription had different regulating effects on cancer cells and normal cells. Conclusion:Tian Bao Zhong Ning San had the effects of inhibiting growth of cancer cells.

目的 :观察“天宝肿宁散”方剂对小鼠成纤维细胞 ( 3T3)和人膀胱癌细胞 ( EJ)、人喉癌细胞( Hep- 2 )、人胃癌细胞 ( MGC- 80 3)、人肝癌细胞 ( Q3)以及人肺癌细胞 ( Spc- A- 1)等肿瘤细胞生物学作用。方法 :采用体外细胞培养法。结果 :显示该方剂具有抑制以上癌细胞的细胞增殖和蛋白质代谢 ,而且可致这些癌细胞的生长形态发生改变 ,但对小鼠 ( 3T3)细胞无明显抑制作用。结论 :该方剂对癌细胞和正常细胞存在不同的生物学作用

Aim To prepare the prodrugs of curcumin,which could be selectively activated in tumor cells,in order to establish a basis for further development of targeted chemotherapy for cancer.Methods Based on the molecular structure of curcumin,the N-maleoyl-L-valine-curcumin(NVC) and N-maleoyl-glycine-curcumin(NGC) were chemically synthesized and identified using IR spectroscopy.After treatment with these two prodrugs for 6~24 h,the growth inhibition rates on human bladder cancer EJ cells and renal tubular epithelial(HKC)...

Aim To prepare the prodrugs of curcumin,which could be selectively activated in tumor cells,in order to establish a basis for further development of targeted chemotherapy for cancer.Methods Based on the molecular structure of curcumin,the N-maleoyl-L-valine-curcumin(NVC) and N-maleoyl-glycine-curcumin(NGC) were chemically synthesized and identified using IR spectroscopy.After treatment with these two prodrugs for 6~24 h,the growth inhibition rates on human bladder cancer EJ cells and renal tubular epithelial(HKC) cells were detected using MTT colorimetry.Results After treatment with 20~40 μmol·L~(-1) NVC and NGC for 6~24 h,the growth inhibitory effects on EJ cells were 6.71%~65.13%%(P<0.05) and 10.96%~73.01%(P<0.05),respectively,in a dose-and time-dependent manner.When compared with the curcumin of same concentrations,the growth inhibitory effects of these two prodrugs on HKC cells were significantly decreased(P<0.01).Conclusion The two curcumin prodrugs,N-maleoyl-L-valine-curcumin and N-maleoyl-glycinecurcumin were chemically synthesized successfully and both could inhibit the growth of tumor cell in vitro.Furthermore,the two prodrugs of curcumin were less toxic in HKC cells than curcumin.

目的制备姜黄素前体药物,使其在肿瘤细胞内高选择性活化,为进一步开展肿瘤靶向性化疗奠定基础。方法以姜黄素分子结构为基础,化学合成N-马来酰-L-缬氨酸酯姜黄素、N-马来酰-甘氨酸酯姜黄素,红外光谱法进行鉴定;MTT比色分析法比较两种姜黄素前体药物作用6~24 h后,人膀胱癌EJ细胞及肾小管上皮HKC细胞生长抑制的差异。结果20~40μmol.L-1的N-马来酰-L-缬氨酸酯姜黄素、N-马来酰-甘氨酸酯姜黄素作用6~24 h后,EJ细胞生长抑制率分别为6.71%~65.13%(P<0.05)、10.96%~73.01%(P<0.05),呈浓度、时间依赖性。与同浓度姜黄素比较,两种前体药物对HKC细胞生长的抑制作用均降低(P<0.01)。结论本研究成功的合成了两种姜黄素的前体药物,N-马来酰-L-缬氨酸酯姜黄素、N-马来酰-甘氨酸酯姜黄素;二者均能体外抑制人膀胱癌EJ细胞增殖,其对人肾小管上皮HKC细胞的抑制毒性作用低于姜黄素。

 
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