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人膀胱癌    
相关语句
  human bladder
    Production of LAK cell-Inducing factor by a human bladder epithelial carcinoma cell line T24
    人膀胱癌上皮细胞株T24分泌LAK细胞诱导因子(LAK—IF)
短句来源
    Application of Electroporation in Transducing Plasmid Plasmid DNA into Human Bladder
    电穿孔质粒DNA转染人膀胱癌细胞系的应用研究
短句来源
    Retrovirus mediated human IL 2 gene transfer in human bladder carcinoma cells
    逆转录病毒介导人白细胞介素2基因在人膀胱癌细胞中的表达
短句来源
    PREPARATION OF THE PHAGE SINGLE CHAIN ANTIBODY OF ANTI HUMAN BLADDER CANCER
    抗人膀胱癌噬菌体单链抗体的初步制备
短句来源
    METHODS: Human bladder cancer cell line T24 was inoculated into the bladders of 25 BALB/c nude mice to establish orthotopic bladder cancer model.
    方法:直视下经尿道机械损伤BALB/c裸鼠膀胱粘膜,将人膀胱癌细胞T24经尿道种植于25只裸鼠膀胱,建立荷人膀胱癌原位动物模型。
短句来源
更多       
  human bladder cancer
    PREPARATION OF THE PHAGE SINGLE CHAIN ANTIBODY OF ANTI HUMAN BLADDER CANCER
    抗人膀胱癌噬菌体单链抗体的初步制备
短句来源
    METHODS: Human bladder cancer cell line T24 was inoculated into the bladders of 25 BALB/c nude mice to establish orthotopic bladder cancer model.
    方法:直视下经尿道机械损伤BALB/c裸鼠膀胱粘膜,将人膀胱癌细胞T24经尿道种植于25只裸鼠膀胱,建立荷人膀胱癌原位动物模型。
短句来源
    Establishment of Balb/c nude mice orthotopic transplantation tumor model with human bladder cancer
    荷人膀胱癌原位移植瘤Balb/c裸小鼠动物模型的建立
短句来源
    This laid the foundation to develop hIL 2 gene transducted tumor vaccine of human bladder cancer.
    转hIL-2基因在人膀胱癌细胞中的高效表达,为人膀胱癌转hIL-2基因瘤苗的应用研究打下了基础。
短句来源
    Rabbits were immunized with the second hybridoma of Ab 1 and F(ab′) 2 fragment. Antiserum, the BfAb against human bladder cancer, was purified through gel and then was identified through ELISA and immunohistochemistry.
    然后将该片段通过二次杂交制备杂交瘤 ,用其免疫新西兰白兔 ,血清经凝胶纯化后获抗人膀胱癌 Bf Ab,应用 ELISA和免疫组化方法进行鉴别。
短句来源
更多       
  human bladder cancer cell
    METHODS: Human bladder cancer cell line T24 was inoculated into the bladders of 25 BALB/c nude mice to establish orthotopic bladder cancer model.
    方法:直视下经尿道机械损伤BALB/c裸鼠膀胱粘膜,将人膀胱癌细胞T24经尿道种植于25只裸鼠膀胱,建立荷人膀胱癌原位动物模型。
短句来源
  human bladder carcinoma
    Retrovirus mediated human IL 2 gene transfer in human bladder carcinoma cells
    逆转录病毒介导人白细胞介素2基因在人膀胱癌细胞中的表达
短句来源
    Total RNA was extracted from human bladder carcinoma cell line 5637 by Guanidine isothiocynate/CsCl method. The first-strand cDNA for G-CSF was synthesized and then amplified by using GeneAmp RNA PCR system with two specific primers synthesized in this laboratory.
    人膀胱癌细胞系(5837)经常规培养至所需数量后,用异硫氰酸胍/氯化铯法提取总RNA,应用CETUS的GeneAmp RNA PCR Kit系统进行逆转录反应合成cDNA第一条链,用DNA合成仪合成G-CSF cDNA的上游和下游特异性引物,以cDNA第一条链为模板进行PCR反应,特异性扩增G-CSF cDNA。
短句来源
    Preparation and identification of bifunctional antibodies against human bladder carcinoma
    抗人膀胱癌双功能抗体的制备与鉴定
短句来源
    By using Liposollleunlediated transfection, eukaryotic expression vector tgCMV/hytk-IRES-gfP was transfected into COS7 cell and human bladder carcinoma cells EJ. The results of PCR and microscopy detection show that the hytk-IRES-GFP gene was successfully transferred into COS7 cell and EJ cells. There were no differences in the growth pattern or the morphology between EJ and EJ/hytk-IRES-GFP cells.
    利用脑炎心肌炎病毒 (encephalomyocarditis virus, EMCV)的内部核糖体进入位点( internal ribosome entry site, IRES),将 gfp 的cDNA与hytk真核表达载体重组,构建获得含gfp和hytk基因的重组质粒:Lipofectin介导下分别转染 COS-7细胞及人膀胱癌细胞株EJ,并检测其表达情况。
短句来源

 

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  human bladder
TBARS, Carnitine, and Reduced Glutathione Levels in Human Bladder Carcinoma
      
Select cytocompatibility experiments (specifically adhesion and long-term growth studies) were performed on these scaffolds using human bladder smooth muscle cells (BdSMCs).
      
Construction and expression of a human-mouse chimeric antibody against human bladder cancer
      
Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer.
      
Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR.
      
更多          
  human bladder cancer
Construction and expression of a human-mouse chimeric antibody against human bladder cancer
      
Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer.
      
Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR.
      
Conclusion: The constructed chimeric antibody was expressed successfully in eukaryotic cells, and the chimeric antibody had desired affinity against human bladder cancer cells.
      
The effects of EPI-CDMN associated with external pulsed electromagnetic fields (PEMFs) (10 mT) on killing human bladder cancer BIU-87 cells were studied by MTT assay and Annexin-V/PI double-labeled flow cytometry technique, respectively.
      
更多          
  human bladder cancer cell
Preliminary study of the in vitro growth inhibition of human bladder cancer cell line BIU-87 by arsenic trioxide
      
The growth inhibition rates of human bladder cancer cell line BIU87 by various concentrations of As2O3 were detected by using MTT method.
      
Adenovirus-mediated transfer of p53 and p16 inhibiting proliferating activity of human bladder cancer cell EJin vitro andin vivo
      
Implantation of human bladder cancer cell lines in the bladder wall of nude rats results in tumor formation, providing an excellent model to test this.
      
Effector cells in natural cytotoxicity against human bladder cancer cell lines
      
更多          
  human bladder carcinoma
TBARS, Carnitine, and Reduced Glutathione Levels in Human Bladder Carcinoma
      
Expression of a mutant hTERT in human bladder carcinoma cell line T24 and its clinical significance
      
Apoptosis induced by ginsenoside Rg3 in a human bladder carcinoma cell line
      
Plasmids encoding each of the mutated SF-1 proteins were cotransfected with the StAR and HDL-R promoter constructs into human bladder carcinoma (HTB-9) cells in the presence or absence of dibutyryl cAMP.
      
Erythroid progenitor cells (BFUe: burst forming units-erythroid) are cultured in 1 ml of 0.35% agarose in IMDM with 30% FBS and conditioned medium from human bladder carcinoma cell line 5637 or recombinant interleukin 3.
      
更多          
  其他


Marked hypercalcemia would be caused by ectodermic solid tumor remote from the bone and Without any indication of bone invasion. It suggested that certain humoral factor secreted by the tumor may contribute to the pathogenesis.We partially purified and characterized bone resorption factor (BRF) from human transitional cell carcinoma (TCC) , rat Walker 256 carcinoma and mouse 7,12 dimethyl benz (a) anthracene (DMBA) induced squamous carcinoma. Gel filtration chromatography of tumor extract demonstrates a single...

Marked hypercalcemia would be caused by ectodermic solid tumor remote from the bone and Without any indication of bone invasion. It suggested that certain humoral factor secreted by the tumor may contribute to the pathogenesis.We partially purified and characterized bone resorption factor (BRF) from human transitional cell carcinoma (TCC) , rat Walker 256 carcinoma and mouse 7,12 dimethyl benz (a) anthracene (DMBA) induced squamous carcinoma. Gel filtration chromatography of tumor extract demonstrates a single major peak of bone resorption activity (BRA, 45Ca release from calvarial bone) that elutes with an apparent MW of about 15,000 dalton. The BRA is associated with increased release of immunoreactive PGE 2 and both can be inhibited by indomethacin and boiling. In TCC and DMBA, the BRA were co-eluted with an activity of stimulating aden-ylate cyclase in rat osteosarcoma cells ROS 17/2.8. It appears that these factors are unique BRF, which are different from other known factors that can cause bone resorption.

某些源自外胚层的骨外实体瘤,它们并未浸润至骨组织,但可引起血钙显著增高,提示这些肿瘤可能分泌体液因子作用于骨导致溶骨。我们从人膀胱癌、大鼠乳腺癌(Walker 256)及7.12-Dimethyl Benz[α]anthracene诱发的小鼠鳞癌的提取液中初步分离鉴定了一种溶骨因子。肿瘤提取液经ultrogel层析,发观仅有一溶骨活性峰(~(45)Ca自乳鼠顶骨培养中的释出率),相当于表观分子量15,000道尔顿。此溶骨活性峰与PGE2生成的活性峰相平行,两者均能被Indomethacin及煮沸所抑制。在膀胱癌及鳞癌中,溶骨活性峰还与刺激大鼠成骨肉瘤细胞腺苷酸环化酶的活性相平行。实验结果表明此溶骨因子不同于其它已知能引起溶骨的因子。

The active ester of chlorambu(?) (CBL) was attached to a monoclonal antibody 5OH·19, against human colorectal cancer cell line (LOVO) to constitute a immunoconjugate CBL-5OH·19. which has a high ratio of CBL per molecule of McAb (25~30: 1) and (?)xcellent targeting activity. The results of (?)0-minute assay and 24-hour assay indicated that CBL-5OH·19 had strong in vitro killing activity to LOVO cells, which was more effective than free CBL. Meanwhile, CBL-5OH·19 was found to have no effect on human bladder tumor...

The active ester of chlorambu(?) (CBL) was attached to a monoclonal antibody 5OH·19, against human colorectal cancer cell line (LOVO) to constitute a immunoconjugate CBL-5OH·19. which has a high ratio of CBL per molecule of McAb (25~30: 1) and (?)xcellent targeting activity. The results of (?)0-minute assay and 24-hour assay indicated that CBL-5OH·19 had strong in vitro killing activity to LOVO cells, which was more effective than free CBL. Meanwhile, CBL-5OH·19 was found to have no effect on human bladder tumor cell line (E-J) and the conjugate CBLOIgG had no killing effect on both of LOVO and E-J cells. These results indicated that CBL-5OH·19was not only effective but also specific in killing the human colorectal cancer cells.

苯丁酸氮芥(CBL)的活性酯通过共价联接的方式与抗人直肠癌单克隆抗体5OH.19偶联,形成的免疫偶联物CBL一5OH.19具有较高的药物/抗体比率(25~30∶1)以及很好的导向活性。在体外30分钟试验和24小时试验中,CBL—50H.19对人直肠癌LoVo细胞均具有很强的体外杀伤活性.其效果比游离的CBL强。CBL—50H.19对人膀胱癌E—J细胞无杀伤活性;CBL与正常小鼠IgG偶联组成的免疫偶联物对LoVo细胞和E—J细胞均无杀伤作用。这表明CBL—50H.19对人直肠癌细胞不但具有较强的杀伤能力.而且具有好的杀伤特异性.

The synergistic effect of combinations of killing agents on tumor cells has long been recognized. Fodstad et al, reported that the combinations of Ricin and Adriamycin have a strong, syn ergistic effect on the L1210 leukemia cells. In this report, a novel immunotoxin containing Ricin and Adriamycin, BDI-l-Ricin-Adriamycin, was described.Ricin and Adriamycin were chemically coupled onto one monoclonal antibody molecule against human bladder cancer, BDI-1, to construct the two-killing-agent containing immunotoxin....

The synergistic effect of combinations of killing agents on tumor cells has long been recognized. Fodstad et al, reported that the combinations of Ricin and Adriamycin have a strong, syn ergistic effect on the L1210 leukemia cells. In this report, a novel immunotoxin containing Ricin and Adriamycin, BDI-l-Ricin-Adriamycin, was described.Ricin and Adriamycin were chemically coupled onto one monoclonal antibody molecule against human bladder cancer, BDI-1, to construct the two-killing-agent containing immunotoxin. The results of indirect immunofluorescence test and competiton bindig assay showed that the retention of 70% of the antibody binding activity in this immunotoxin was obtained. The in vitro cytotoxicity assay indicated that the immunotoxin in the presence of 0.1M galactose is 30,000 times more cytotoxic than BDI-1-Adriamycin conjugate and 10 times more cytotoxic than BDI-1-Ricin immunotoxin in the bladder cancer-specific killing, but has no cytotoxic effect on the LOVO colon carcinoma cells. This two-killing-agent containing immunotoxin may lead to the development of a new kind of immunotoxins which have strong cytotoxic activity to target cells.

本文用化学偶联方法,将篦麻毒素(RiCin)及阿霉素(Adriamycin,Adr)同时偶联到抗人膀胱癌单克隆抗体分子上,构建了第一个具有“双单头”的抗肿瘤导向药物。经间接免疫荧光检测以及放射竞争结合试验证明,这个免疫毒素保持了原抗体活性的70%。体外杀伤试验的结果表明,这个双单头免疫毒素在0.1M半乳糖存在的条件下,对膀胱癌细胞BIU-87的体外杀伤作用比单抗-阿霉索强30000倍;比单抗-篦麻毒素强10倍。对无关的人直肠癌LoVo细胞无明显的杀伤作用。

 
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