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   人膀胱癌 在 生物医学工程 分类中 的翻译结果: 查询用时:0.091秒
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人膀胱癌
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  human bladder
    Method:The photodynamic cytotoxicity of human bladder cancer cells(T-24 and SCaBER) was studied by MTT rapid colormetric assay referring to different CPD 4 concentration,laser energy and wavelength.
    方法:采用MTT比色分析法判别体外人膀胱癌T-24和SCaBER细胞对不同的激光波长、能量和CPD4浓度的光敏效应。
短句来源
    The results of measurement showed that the parameters of optical properties of human normal bladder and human bladder cancer tissue at 808 nm laser and the linearly polarized laser in Kubelka-Munk two-flux model had prominent distinction (P<0.01).
    结果表明 ,Kubelka Munk二流模型下 ,人正常膀胱组织与人膀胱癌组织对 80 8nm激光及其线偏振激光的各个光学特性参量均有非常显著的差异 (P <0 0 1)。
短句来源
    CONCLUSION: SDIO N could connect with GFP plasmid by oxidation-reduction reaction,and success to transfer GFP gene into human bladder cancer BIU-87 cells in vitro.
    结论:SDION可通过氧化还原反应与GFP质粒相连,在体外可将GFP基因成功转染入人膀胱癌BIU-87细胞。
短句来源
    Objective:The efficacy of laser wavelength, energy and CPD 4 concentration was studied in vitro photodynamic therapy(PDT) of the human bladder cancer cells.
    目的:探索叶绿素衍生物(CPD4)光动力学疗法在体外人膀胱癌细胞的杀伤效应中不同的激光波长、能量和CPD4浓度之间的关系。
短句来源
    All parameters of optical properties of human bladder cancer tissue for 808 nm laser and the linearly polarized laser had not prominent distinction(P>0.05) which showed that the depolarization of human bladder cancer tissue to 808 nm linearly polarized irradiation was very big.
    人膀胱癌组织对 80 8nm激光及其线偏振激光的光学特性参量均没有显著性差异 (P >0 0 5 ) ,表明人膀胱癌组织对 80 8nm线偏振激光的退偏振较大。
短句来源
  human bladder cancer
    Method:The photodynamic cytotoxicity of human bladder cancer cells(T-24 and SCaBER) was studied by MTT rapid colormetric assay referring to different CPD 4 concentration,laser energy and wavelength.
    方法:采用MTT比色分析法判别体外人膀胱癌T-24和SCaBER细胞对不同的激光波长、能量和CPD4浓度的光敏效应。
短句来源
    The results of measurement showed that the parameters of optical properties of human normal bladder and human bladder cancer tissue at 808 nm laser and the linearly polarized laser in Kubelka-Munk two-flux model had prominent distinction (P<0.01).
    结果表明 ,Kubelka Munk二流模型下 ,人正常膀胱组织与人膀胱癌组织对 80 8nm激光及其线偏振激光的各个光学特性参量均有非常显著的差异 (P <0 0 1)。
短句来源
    CONCLUSION: SDIO N could connect with GFP plasmid by oxidation-reduction reaction,and success to transfer GFP gene into human bladder cancer BIU-87 cells in vitro.
    结论:SDION可通过氧化还原反应与GFP质粒相连,在体外可将GFP基因成功转染入人膀胱癌BIU-87细胞。
短句来源
    Objective:The efficacy of laser wavelength, energy and CPD 4 concentration was studied in vitro photodynamic therapy(PDT) of the human bladder cancer cells.
    目的:探索叶绿素衍生物(CPD4)光动力学疗法在体外人膀胱癌细胞的杀伤效应中不同的激光波长、能量和CPD4浓度之间的关系。
短句来源
    All parameters of optical properties of human bladder cancer tissue for 808 nm laser and the linearly polarized laser had not prominent distinction(P>0.05) which showed that the depolarization of human bladder cancer tissue to 808 nm linearly polarized irradiation was very big.
    人膀胱癌组织对 80 8nm激光及其线偏振激光的光学特性参量均没有显著性差异 (P >0 0 5 ) ,表明人膀胱癌组织对 80 8nm线偏振激光的退偏振较大。
短句来源
  “人膀胱癌”译为未确定词的双语例句
    In this paper, a double-integrating-spheres system, basic principle of measuring technology of ray radiation, optical model of biological tissues were used to study the optical properties of human tissues.
    采用双积分球系统和光辐射测量技术的基本原理以及运用生物组织的光学模型 ,研究了 80 8nm激光及其线偏振激光辐照人正常小肠、人正常膀胱和人膀胱癌组织的光学特性。
短句来源
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  human bladder
TBARS, Carnitine, and Reduced Glutathione Levels in Human Bladder Carcinoma
      
Select cytocompatibility experiments (specifically adhesion and long-term growth studies) were performed on these scaffolds using human bladder smooth muscle cells (BdSMCs).
      
Construction and expression of a human-mouse chimeric antibody against human bladder cancer
      
Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer.
      
Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR.
      
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  human bladder cancer
Construction and expression of a human-mouse chimeric antibody against human bladder cancer
      
Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer.
      
Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR.
      
Conclusion: The constructed chimeric antibody was expressed successfully in eukaryotic cells, and the chimeric antibody had desired affinity against human bladder cancer cells.
      
The effects of EPI-CDMN associated with external pulsed electromagnetic fields (PEMFs) (10 mT) on killing human bladder cancer BIU-87 cells were studied by MTT assay and Annexin-V/PI double-labeled flow cytometry technique, respectively.
      
更多          


Objective:The efficacy of laser wavelength, energy and CPD 4 concentration was studied in vitro photodynamic therapy(PDT) of the human bladder cancer cells.Method:The photodynamic cytotoxicity of human bladder cancer cells(T-24 and SCaBER) was studied by MTT rapid colormetric assay referring to different CPD 4 concentration,laser energy and wavelength.Results:The photodynamic cytotoxicity of the CPD 4 was directly correlated to the laser energy and concentration of photosensitizer.Under identical laser energy...

Objective:The efficacy of laser wavelength, energy and CPD 4 concentration was studied in vitro photodynamic therapy(PDT) of the human bladder cancer cells.Method:The photodynamic cytotoxicity of human bladder cancer cells(T-24 and SCaBER) was studied by MTT rapid colormetric assay referring to different CPD 4 concentration,laser energy and wavelength.Results:The photodynamic cytotoxicity of the CPD 4 was directly correlated to the laser energy and concentration of photosensitizer.Under identical laser energy and CPD 4 concentration conditions,the photodynamic cytotoxicity of green light(wavelenght,510nm) was significantly stronger than red light(wavelength,630nm).Conclusions:CPD 4-PDT is effective for killing the human bladder cancer cells in vitro.Green light is stronger than the red light in photodynamic cytotoxicity.These data provide the basis in clinical photodynamic therapy of early lesions of bladder cancer and in preventing postoperative recurrences.The green light is preferable to the red light.

目的:探索叶绿素衍生物(CPD4)光动力学疗法在体外人膀胱癌细胞的杀伤效应中不同的激光波长、能量和CPD4浓度之间的关系。方法:采用MTT比色分析法判别体外人膀胱癌T-24和SCaBER细胞对不同的激光波长、能量和CPD4浓度的光敏效应。结果:CPD4的光敏效应与激光能量和药物浓度呈正相关,并在相同激光能量和药物浓度的情况下,绿光(510nm)的光敏效应明显强于红光(630nm)。结论:CPD4光动力学作用对体外人膀胱细胞癌有明显杀伤效应,而绿光明显强于红光,这为临床采用绿光治疗膀胱癌早期病变和预防术后肿瘤复发提供了实验依据

In this paper, a double-integrating-spheres system, basic principle of measuring technology of ray radiation, optical model of biological tissues were used to study the optical properties of human tissues. The results of measurement showed that the parameters of optical properties of human normal bladder and human bladder cancer tissue at 808 nm laser and the linearly polarized laser in Kubelka-Munk two-flux model had prominent distinction (P<0.01). The parameters of optical properties of human normal bladder...

In this paper, a double-integrating-spheres system, basic principle of measuring technology of ray radiation, optical model of biological tissues were used to study the optical properties of human tissues. The results of measurement showed that the parameters of optical properties of human normal bladder and human bladder cancer tissue at 808 nm laser and the linearly polarized laser in Kubelka-Munk two-flux model had prominent distinction (P<0.01). The parameters of optical properties of human normal bladder tissue for 808 nm laser and the linearly polarized laser had all distinction. Absorption coefficient and effective attenuation coefficient among all parameters of optical properties of human normal bladder tissue had obviously distinction. All parameters of optical properties of human bladder cancer tissue for 808 nm laser and the linearly polarized laser had not prominent distinction(P>0.05) which showed that the depolarization of human bladder cancer tissue to 808 nm linearly polarized irradiation was very big. The parameters of optical properties of human normal small intestine tissue at 808 nm laser and the linearly polarized laser irradiation had all prominent distinction (P<0.01), which showed that the depolarization of human normal small intestine tissue to 808 nm linearly polarized irradiation was smaller. Distribution of light intensity of three kinds of human tissues at 808 nm laser and the linearly polarized laser irradiation in Kubelka-Munk two-flux model had obviously dissimilitude.

采用双积分球系统和光辐射测量技术的基本原理以及运用生物组织的光学模型 ,研究了 80 8nm激光及其线偏振激光辐照人正常小肠、人正常膀胱和人膀胱癌组织的光学特性。结果表明 ,Kubelka Munk二流模型下 ,人正常膀胱组织与人膀胱癌组织对 80 8nm激光及其线偏振激光的各个光学特性参量均有非常显著的差异 (P <0 0 1)。人正常膀胱组织对 80 8nm激光及其线偏振激光的光学特性参量均有差异 ,其中吸收系数和有效衰减系数有明显差异。人膀胱癌组织对 80 8nm激光及其线偏振激光的光学特性参量均没有显著性差异 (P >0 0 5 ) ,表明人膀胱癌组织对 80 8nm线偏振激光的退偏振较大。人正常小肠组织对 80 8nm激光及其线偏振激光的光学特性参量均有显著性差异 (P <0 0 1) ,表明人正常小肠组织对 80 8nm线偏振激光的退偏振较小。在Kubelka Munk二流模型下 ,80 8nm激光及其线偏振激光辐照三种人组织的光强分布均有明显的不同

BACKGROUND &OBJECTIVE: Application of magnetic nano-particles as gene carrier in gene therapy of tumor has developed quickly. To obtain a new typ e non-viral gene introduction and therapy system,which is convenient,and can dr ive target gene to express highly and stably,this study was designed to explore the preparation of superparamagnetic dextran iron oxide nanoparticles(SDION),and the feasibility of SDION used as gene carrier in vitro. METHODS: SDION were pre pared by chemical co-precipitation,separated by...

BACKGROUND &OBJECTIVE: Application of magnetic nano-particles as gene carrier in gene therapy of tumor has developed quickly. To obtain a new typ e non-viral gene introduction and therapy system,which is convenient,and can dr ive target gene to express highly and stably,this study was designed to explore the preparation of superparamagnetic dextran iron oxide nanoparticles(SDION),and the feasibility of SDION used as gene carrier in vitro. METHODS: SDION were pre pared by chemical co-precipitation,separated by gel filtration chromatography o n Sephacryl S-300HR,and centrifugation techniques,characterized by transmission electron microscopy,laser scattering system,and vibrating sample magnetometer s ignal processor. The green fluorescent protein-C2 (GFP-C2) plasmid was used as target gene. SDION-GFP-C2 compounds were synthesized by oxidation-reduction reaction. The connection rate of SDION and GFP-C2 was analyzed by agarose elect rophoresis,and evaluated by measuring concentration of GFP in the supernatant af ter centrifugation. Liposome transfection was used as control,the efficiencies o f SDION and liposome in transferring GFP gene into human bladder cancer BIU-87 cells were evaluated under fluorescence microscope in vitro. RESULTS: The diamet er of SDION ranged from 3 nm to 8 nm,the effective diameter was 59.2 nm,and the saturation magnetization was 0.23 emu/g. After oxidized by sodium periodate of 1 0 mmol/L,and deoxidized by sodium hydride boron of 0.5 mol/L,SDION could connect with GFP in maximum degree,the transfection efficiency of SDION as gene carrier was about 45%,even higher than that of liposome (about 30%). CONCLUSION: SDIO N could connect with GFP plasmid by oxidation-reduction reaction,and success to transfer GFP gene into human bladder cancer BIU-87 cells in vitro.

背景与目的:磁性纳米颗粒作为基因载体在肿瘤基因治疗中的应用得到了迅速发展。为了能获得驱动目的基因高效稳定表达、安全无害、靶向性高、简便的新型非病毒型基因导入和治疗系统,本研究探讨超顺磁性葡聚糖氧化铁纳米颗粒(superparamagneticdextranironoxidenanoparticles,SDION)的制备及其作为体外基因载体的可行性。方法:采用化学共沉淀法制作SDION,通过丙烯葡聚糖凝胶S-300HR色谱和离心法分离SDION,用透射电镜、粒度分析仪和磁力计对SDION进行分析。以绿色荧光蛋白(GFP-C2)质粒为靶基因,通过氧化还原法构建SDION-GFP-C2复合物,用紫外分光光度计和琼脂糖凝胶电泳检测两者的结合率。以脂质体转染作为对照,荧光显微镜分别观察SDION和脂质体体外转染GFP-C2入膀胱癌细胞BIU-87的转染效率。结果:SDION直径在3~8nm之间,有效粒径为59.2nm,比饱和磁化强度为0.23emu/g。分别经10mmol/L的高碘酸钠氧化、0.5mol/L的硼氢化钠还原作用后的SDION和GFP的结合比例最大,SDION对GFPDNA的转染效率为45%左右,明显高于脂...

背景与目的:磁性纳米颗粒作为基因载体在肿瘤基因治疗中的应用得到了迅速发展。为了能获得驱动目的基因高效稳定表达、安全无害、靶向性高、简便的新型非病毒型基因导入和治疗系统,本研究探讨超顺磁性葡聚糖氧化铁纳米颗粒(superparamagneticdextranironoxidenanoparticles,SDION)的制备及其作为体外基因载体的可行性。方法:采用化学共沉淀法制作SDION,通过丙烯葡聚糖凝胶S-300HR色谱和离心法分离SDION,用透射电镜、粒度分析仪和磁力计对SDION进行分析。以绿色荧光蛋白(GFP-C2)质粒为靶基因,通过氧化还原法构建SDION-GFP-C2复合物,用紫外分光光度计和琼脂糖凝胶电泳检测两者的结合率。以脂质体转染作为对照,荧光显微镜分别观察SDION和脂质体体外转染GFP-C2入膀胱癌细胞BIU-87的转染效率。结果:SDION直径在3~8nm之间,有效粒径为59.2nm,比饱和磁化强度为0.23emu/g。分别经10mmol/L的高碘酸钠氧化、0.5mol/L的硼氢化钠还原作用后的SDION和GFP的结合比例最大,SDION对GFPDNA的转染效率为45%左右,明显高于脂质体的转染效率(30%左右)。结论:SDION可通过氧化还原反应与GFP质粒相连,在体外可将GFP基因成功转染入人膀胱癌BIU-87细胞。

 
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