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virus glycoprotein
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  病毒糖蛋白
     After IPTG induction,ELISA results showed that both fusion proteins could bind specifically to the hantavirus nucleoprotein specific mAb, and the fusion protein GSTG1S0.7 could bind Hantaan virus glycoprotein specific mAb.
     经IPTG诱导后,ELISA活性测定结果表明,两种融合蛋白均可与抗汉坦病毒NP的mAb特异性结合,融合蛋白GST-G1S0.7还可与抗汉滩病毒糖蛋白的mAb特异性结合。
短句来源
     Expression and purification of varicella-zoster virus glycoprotein I gene in insect cells
     水痘-带状疱疹病毒糖蛋白I基因在昆虫细胞中的表达及纯化
短句来源
     Construction of prokaryotic expression vector of varicella-zoster virus glycoprotein E and the purification of its expressed product
     水痘-带状疱疹病毒糖蛋白E原核表达载体的表达及产物纯化
短句来源
     The recombinant adenovirus carrying Hantaan virus glycoprotein G 2 gene was obtained, whose titer was 10 10 pfu/mL, and Hantaan virus glycoprotein G 2 was detected in infected HEK293 cells.
     得到了含汉滩病毒G2 基因的重组腺病毒 ,其滴度约为 1 0 10 pfu/mL ,同时在感染的HEK2 93细胞中检测到汉滩病毒糖蛋白G2 的表达。
短句来源
     Construction of the eukaryotic expression vector containing gene of varicella-zoster virus glycoprotein E
     水痘-带状疱疹病毒糖蛋白E表达载体的构建
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  “virus glycoprotein”译为未确定词的双语例句
     Construction of adenoviral vector carrying Hantaan virus glycoprotein G1gene and it's expression in Vero E6 cells
     汉坦病毒囊膜糖蛋白G1基因重组腺病毒载体的构建及其在Vero E6细胞中的表达
短句来源
     A recombinant plasmid comprising adenovirus genome with rabies virus glycoprotein gene in E1 region was constructed by homologous recombination.
     利用大肠杆菌内质粒间同源重组的方法,将狂犬病病毒G基因插入腺病毒基因组的E1基因区,构建了带有狂犬病病毒G基因的重组腺病毒质粒。
短句来源
     Construction and Expression of Recombinant Adenovirus Carrying Hantaan Virus Glycoprotein G_2 Gene
     汉滩病毒囊膜糖蛋白G_2基因重组腺病毒的构建与表达
短句来源
     Expression and Immunologenic Study of the Hantaan Virus Glycoprotein G1 and G2 by Using the Adenoviral Vector
     汉滩病毒囊膜糖蛋白G1、G2腺病毒载体的表达及免疫分析
短句来源
     Construction of yeast expression vector of rubella virus glycoprotein E1 gene
     风疹病毒包膜糖蛋白E1酵母表达载体的构建
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  相似匹配句对
     Identification of glycoprotein C of herpes simplex virus
     放射免疫沉淀试验鉴定单纯疱疹病毒糖蛋白C
短句来源
     Progress on Envelope Glycoprotein D of Pseudorabies virus
     伪狂犬病病毒囊膜蛋白gD研究进展
短句来源
     On Jewish Virus
     计算机病毒“犹太人”
短句来源
     Virus counter
     病毒计数器
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  virus glycoprotein
Inhibition of rabies virus replication was detected in cell culture using an ELISA for detection of rabies virus glycoprotein expression on the cell surface and immunofluorescence for detection of intracellular rabies virus N expression.
      
Characterization of the simian varicella virus glycoprotein C, which is nonessential for in vitro replication
      
Expression of the simian varicella virus glycoprotein L and H
      
Characterization of B virus glycoprotein antibodies induced by DNA immunization
      
Rubella virus glycoprotein interaction with the endoplasmic
      
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in this paper, we reported the results obtained from two trans feetingmethods of Rabies Virus glycoprotein. Comparative studies apparently showed thatLipofectingTM Reagent Method is superior to Calsium Phosphate PrecipitateMethod.

两种方法转染狂犬病毒糖蛋白基因的比较研究李萍萍,吴柏春(武汉生物制品研究所)(华中师范大学昆虫学研究所)关键词转染;Lipofectin ̄TMReagent;重组病毒中图分类号Q785转染是分子生物学中常用的重要技术之一.目前较为普遍使用的转染方法有...

A vaccinia recombinant containing the glycoprotein (G) gene of Chinese Rabies Strain (5aG), which was controlled under the vaccinia virus promoter p11, was constructed by inserting the G gene into the TK region of vaccinia virus.Through indirect immunofluorescence and western blot assays, it was shown that the recombinant VVaG can successfully express the rabies virus glycoprotein, and its molecular weight is was shown that the recombinant VVaG can induce rabies virus neutralizing antibodies (VNA)...

A vaccinia recombinant containing the glycoprotein (G) gene of Chinese Rabies Strain (5aG), which was controlled under the vaccinia virus promoter p11, was constructed by inserting the G gene into the TK region of vaccinia virus.Through indirect immunofluorescence and western blot assays, it was shown that the recombinant VVaG can successfully express the rabies virus glycoprotein, and its molecular weight is was shown that the recombinant VVaG can induce rabies virus neutralizing antibodies (VNA) in all immunized mice and protect them against lethal challege of rabies virus aG and CVS strain intracerebally,and the highest titer of rabies VNA Can reach to 4169 on the 21th day post immunization.

将克隆到的中国狂犬病毒疫苗株(5aG)的糖蛋白基因重组到痘苗病毒TK区,并在痘苗病毒P11启动子的控制下,构建了狂犬-痘苗重组病毒(VVaG)。经间接免疫荧光和Western免疫印染证明,重组病毒VVaG能良好地表达狂犬病毒糖蛋白,其分子量约为6600。用VVaG免疫小鼠,7d便可诱生较高的狂犬病毒中和抗体,21d达4169,并能100%保护狂犬病毒本毒株和国际标准攻击毒(CVS)的致死量攻击。

Expressing vector, pMT010/A+ -Rgp, was constructed by cloning rabies virus glycoprotein( Rgp)cDNA into downstream of the metallothionein promoter of pMT010/A+(ovine).The 2.76 kb Eco R I +Sal I fragments containing Rgp cDNA of pMT010/A+-Rgp were recovered and purified for microinjecting into male pronuclei of fertilized mouse eggs.Of 44 mice developed from these eggs, nine carried the gene of in- terest,which was identified by PCR, Southern hybridization and in situ hybridization. All of transgenic mice...

Expressing vector, pMT010/A+ -Rgp, was constructed by cloning rabies virus glycoprotein( Rgp)cDNA into downstream of the metallothionein promoter of pMT010/A+(ovine).The 2.76 kb Eco R I +Sal I fragments containing Rgp cDNA of pMT010/A+-Rgp were recovered and purified for microinjecting into male pronuclei of fertilized mouse eggs.Of 44 mice developed from these eggs, nine carried the gene of in- terest,which was identified by PCR, Southern hybridization and in situ hybridization. All of transgenic mice transmit their transgene to 50%of their offsprings indicative of Mendelian inheritance, Six out of 9 lines were designated as TgN (oMT- Rgp)1 Lge,TgN(oMT-Rgp)2Lge…TgN(oMT-Rgp)6Lge.

将狂犬病病毒糖蛋白cDVABglⅡ(1.67kb)片段正向插入pMT010/A+BamHⅠ切点,构建重组质粒pMT010/A+-Rgp,用EcoRⅠ+SalⅠ对pMT010/A+-Rgp进行双酶切后,回收含有狂犬病病毒糖蛋白cDNA和绵羊MT启动子的2.76kb片段,通过显微注射技术将该片段注入小鼠单细胞受精卵雄前核内,在进行胚胎移植后,获得44只小鼠,经PCR、South-ern杂交及原位杂交检测,证明有9只携带有目的基因。目的基因(即转基因)按孟德尔特性遗传给其后代,由此相应建立了9个转基因鼠系,对其中6个鼠系分别命名为TgN(oMT-Rgp)ILge、TgN(oMT-Rgp)2Lge……TgN(oMT-Rgp)6Lge。

 
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