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virus glycoprotein
相关语句
  病毒糖蛋白
    Transgenic Mice Expressing Rabies Virus Glycoprotein Developed Immune Tolerance to Rabies Viruses
    狂犬病病毒糖蛋白转基因小鼠对该病毒形成免疫耐受
短句来源
    EXPRESSION OF MAREK'S DISEASE VIRUS GLYCOPROTEIN D GENE TRANSFERRED INTO CHICKEN EMBRYO FIBROBLAST GENOME BY USING RETROVIRAL VECTOR
    由反转录病毒载体转移的马立克病毒糖蛋白D基因在感染细胞中的表达
短句来源
    Construction of Recombinant Canine Adenovirus Type-2 Expressing Rabies Virus Glycoprotein
    表达狂犬病病毒糖蛋白的重组犬2型腺病毒的构建
短句来源
    Cloning and expression of the vesicular stomatitis virus glycoprotein gene
    水疱性口炎病毒糖蛋白基因的克隆和表达
短句来源
    Construction of the eukaryotic expression vector containing gene of varicella-zoster virus glycoprotein E
    水痘-带状疱疹病毒糖蛋白E表达载体的构建
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  “virus glycoprotein”译为未确定词的双语例句
    Eucaryotic Expression and Biological Activities of Rubella Virus Glycoprotein E1
    风疹病毒包膜糖蛋白E1的真核表达及其生物学活性研究
短句来源
    Analysis of amino acid sequences of antigenic domains of the rabies virus glycoprotein
    狂犬病毒糖蛋白抗原决定簇的筛选及其氨基酸序列分析
短句来源
    Observation on Immunogenic Effect of Expression of the Rabies Virus Glycoprotein by Recombinant Vaccinia Virus (422 Strain)
    狂犬病毒糖蛋白重组痘苗病毒“422”株的免疫效果观察
短句来源
    STUDY ON BIOLOGICAL CHARACTERISTICS OF RECOMBINANT VACCINIA VIRUS CONTAING RABIES VIRUS GLYCOPROTEIN GENE
    含狂犬病毒糖蛋白基因的重组痘苗病毒(RTTVV-3)传代后生物学特性研究
短句来源
    Isolation of Vaccinia Virus Recombinants Expressing Rabies Virus Glycoprotein by Lac Gene
    用Lac基因筛选表达狂犬病毒糖蛋白的重组痘苗病毒
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  virus glycoprotein
Inhibition of rabies virus replication was detected in cell culture using an ELISA for detection of rabies virus glycoprotein expression on the cell surface and immunofluorescence for detection of intracellular rabies virus N expression.
      
Characterization of the simian varicella virus glycoprotein C, which is nonessential for in vitro replication
      
Expression of the simian varicella virus glycoprotein L and H
      
Characterization of B virus glycoprotein antibodies induced by DNA immunization
      
Rubella virus glycoprotein interaction with the endoplasmic
      
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in this paper, we reported the results obtained from two trans feetingmethods of Rabies Virus glycoprotein. Comparative studies apparently showed thatLipofectingTM Reagent Method is superior to Calsium Phosphate PrecipitateMethod.

两种方法转染狂犬病毒糖蛋白基因的比较研究李萍萍,吴柏春(武汉生物制品研究所)(华中师范大学昆虫学研究所)关键词转染;Lipofectin ̄TMReagent;重组病毒中图分类号Q785转染是分子生物学中常用的重要技术之一.目前较为普遍使用的转染方法有...

A vaccinia recombinant containing the glycoprotein (G) gene of Chinese Rabies Strain (5aG), which was controlled under the vaccinia virus promoter p11, was constructed by inserting the G gene into the TK region of vaccinia virus.Through indirect immunofluorescence and western blot assays, it was shown that the recombinant VVaG can successfully express the rabies virus glycoprotein, and its molecular weight is was shown that the recombinant VVaG can induce rabies virus neutralizing antibodies (VNA)...

A vaccinia recombinant containing the glycoprotein (G) gene of Chinese Rabies Strain (5aG), which was controlled under the vaccinia virus promoter p11, was constructed by inserting the G gene into the TK region of vaccinia virus.Through indirect immunofluorescence and western blot assays, it was shown that the recombinant VVaG can successfully express the rabies virus glycoprotein, and its molecular weight is was shown that the recombinant VVaG can induce rabies virus neutralizing antibodies (VNA) in all immunized mice and protect them against lethal challege of rabies virus aG and CVS strain intracerebally,and the highest titer of rabies VNA Can reach to 4169 on the 21th day post immunization.

将克隆到的中国狂犬病毒疫苗株(5aG)的糖蛋白基因重组到痘苗病毒TK区,并在痘苗病毒P11启动子的控制下,构建了狂犬-痘苗重组病毒(VVaG)。经间接免疫荧光和Western免疫印染证明,重组病毒VVaG能良好地表达狂犬病毒糖蛋白,其分子量约为6600。用VVaG免疫小鼠,7d便可诱生较高的狂犬病毒中和抗体,21d达4169,并能100%保护狂犬病毒本毒株和国际标准攻击毒(CVS)的致死量攻击。

Six hybridoma cell strains secreting McAbs were obtained by immunizing BALB/c mice with CVS strain of rabies virus. We found that 4 of the 6 strains secreted McAbs to rabies virus glycoprotein, and the other 2 of them secreted McAbs to rabies virus nucleoprotein. The dignostic reagent for rabies virus by sandwich ELISA was prepared with the ascites containing these McAbs and showed good specificity to both fixed and street virus strains, but no non-specific reaction to normal mice brain...

Six hybridoma cell strains secreting McAbs were obtained by immunizing BALB/c mice with CVS strain of rabies virus. We found that 4 of the 6 strains secreted McAbs to rabies virus glycoprotein, and the other 2 of them secreted McAbs to rabies virus nucleoprotein. The dignostic reagent for rabies virus by sandwich ELISA was prepared with the ascites containing these McAbs and showed good specificity to both fixed and street virus strains, but no non-specific reaction to normal mice brain or tissue ctllture was found. The CVS strain with a low titer of 330 LD50 was detectable and this indicated its good sensitivity.

狂犬病毒CVS株免疫BALB/c小鼠后,获得6株分泌单克隆抗体的杂交瘤细胞株,经检测,其中4株分泌抗狂犬病毒核蛋白单抗.2株分泌抗糖蛋白单抗.将这些单抗腹水制成狂犬病毒ELISA夹心法酶标诊断试剂,试验结果表明,对固定每株及街毒有较好的特异性.对正常鼠脑及组织培养物无非特异性反应,可检出毒力低至330LD50的CVS林狂犬病毒,有较好的敏感性。

 
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