助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   virus glycoprotein 在 畜牧与动物医学 分类中 的翻译结果: 查询用时:0.531秒
图标索引 在分类学科中查询
所有学科
畜牧与动物医学
生物学
基础医学
蚕蜂与野生动物保护
皮肤病与性病
更多类别查询

图标索引 历史查询
 

virus glycoprotein
相关语句
  病毒糖蛋白
    RESTRICTION ENZYME ANALYSIS OF MAREK’S DISEASE VIRUS GLYCOPROTEIN B ANTIGEN GENE CLONE
    马立克病病毒糖蛋白B抗原基因克隆的酶切分析
短句来源
    Study on Construction of Transgenic Mice Carrying Ra-bies Virus Glycoprotein cDNA
    狂犬病病毒糖蛋白转基因小鼠的构建研究
短句来源
    Expression of Rabies Virus Glycoprotein Gene in E. coli
    狂犬病病毒糖蛋白基因在原核细胞中的表达
短句来源
    STUDY ON INTRODUCTION OF MAREK’S DISEASE VIRUS GLYCOPROTEIN D GENE TO GENOMIC DNA OF INFECTED CELLS
    马立克病毒糖蛋白D基因转入感染细胞DNA的研究
短句来源
    Transgenic Mice Expressing Rabies Virus Glycoprotein Developed Immune Tolerance to Rabies Viruses
    狂犬病病毒糖蛋白转基因小鼠对该病毒形成免疫耐受
短句来源
更多       
  “virus glycoprotein”译为未确定词的双语例句
    A recombinant plasmid comprising adenovirus genome with rabies virus glycoprotein gene in E1 region was constructed by homologous recombination.
    利用大肠杆菌内质粒间同源重组的方法,将狂犬病病毒G基因插入腺病毒基因组的E1基因区,构建了带有狂犬病病毒G基因的重组腺病毒质粒。
短句来源
    Combined immunization of recombinant fowlpox virus containing CTN strain rabies virus glycoprotein gene and nucleic acid vaccine
    狂犬病毒CTN株糖蛋白重组鸡痘病毒与核酸疫苗的联合免疫
短句来源
    Advance in Infectious bovine rhinotracheitis virus Glycoprotein B
    牛传染性鼻气管炎病毒gB蛋白研究进展
短句来源
    Molecular Characterisation of Marek's Disease Virus Glycoprotein I Gene
    马立克氏病病毒囊膜糖蛋白gI基因的分子生物学特性
短句来源
    Expression of Bovine Ephemeral Fever Virus Glycoprotein G Gene and Bovine Interleukine-2 Gene in Recombinant Vaccinia Virus
    牛流行热病毒JB76H株G蛋白及牛白细胞介素-2基因在重组痘苗病毒中的表达
短句来源
更多       
查询“virus glycoprotein”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  virus glycoprotein
Inhibition of rabies virus replication was detected in cell culture using an ELISA for detection of rabies virus glycoprotein expression on the cell surface and immunofluorescence for detection of intracellular rabies virus N expression.
      
Characterization of the simian varicella virus glycoprotein C, which is nonessential for in vitro replication
      
Expression of the simian varicella virus glycoprotein L and H
      
Characterization of B virus glycoprotein antibodies induced by DNA immunization
      
Rubella virus glycoprotein interaction with the endoplasmic
      
更多          


Expressing vector, pMT010/A+ -Rgp, was constructed by cloning rabies virus glycoprotein( Rgp)cDNA into downstream of the metallothionein promoter of pMT010/A+(ovine).The 2.76 kb Eco R I +Sal I fragments containing Rgp cDNA of pMT010/A+-Rgp were recovered and purified for microinjecting into male pronuclei of fertilized mouse eggs.Of 44 mice developed from these eggs, nine carried the gene of in- terest,which was identified by PCR, Southern hybridization and in situ hybridization. All of transgenic mice...

Expressing vector, pMT010/A+ -Rgp, was constructed by cloning rabies virus glycoprotein( Rgp)cDNA into downstream of the metallothionein promoter of pMT010/A+(ovine).The 2.76 kb Eco R I +Sal I fragments containing Rgp cDNA of pMT010/A+-Rgp were recovered and purified for microinjecting into male pronuclei of fertilized mouse eggs.Of 44 mice developed from these eggs, nine carried the gene of in- terest,which was identified by PCR, Southern hybridization and in situ hybridization. All of transgenic mice transmit their transgene to 50%of their offsprings indicative of Mendelian inheritance, Six out of 9 lines were designated as TgN (oMT- Rgp)1 Lge,TgN(oMT-Rgp)2Lge…TgN(oMT-Rgp)6Lge.

将狂犬病病毒糖蛋白cDVABglⅡ(1.67kb)片段正向插入pMT010/A+BamHⅠ切点,构建重组质粒pMT010/A+-Rgp,用EcoRⅠ+SalⅠ对pMT010/A+-Rgp进行双酶切后,回收含有狂犬病病毒糖蛋白cDNA和绵羊MT启动子的2.76kb片段,通过显微注射技术将该片段注入小鼠单细胞受精卵雄前核内,在进行胚胎移植后,获得44只小鼠,经PCR、South-ern杂交及原位杂交检测,证明有9只携带有目的基因。目的基因(即转基因)按孟德尔特性遗传给其后代,由此相应建立了9个转基因鼠系,对其中6个鼠系分别命名为TgN(oMT-Rgp)ILge、TgN(oMT-Rgp)2Lge……TgN(oMT-Rgp)6Lge。

The rabies virus glycoprotein gene was cloned into the Bam HI site ofhigh level expressing vectors-pET-17b and pET-17b2. And pET-17b2 was reconstructed from pET-17b by Nde Ⅰ- Sac Ⅱ Ⅰ.Two recombinant plasmids,pET-17RVgpand pET-17b2RVgp were gotten.They were transferred into expressing host cells E。coli BL21 (DE3)and BL21(DE3)plysS strains respectively.Inducing with IPTG,theyall express rabies virus glycoprotein which is about 60 000 dalton in molecular weight bySDS-pAGE.Rabies virus glycoprotein...

The rabies virus glycoprotein gene was cloned into the Bam HI site ofhigh level expressing vectors-pET-17b and pET-17b2. And pET-17b2 was reconstructed from pET-17b by Nde Ⅰ- Sac Ⅱ Ⅰ.Two recombinant plasmids,pET-17RVgpand pET-17b2RVgp were gotten.They were transferred into expressing host cells E。coli BL21 (DE3)and BL21(DE3)plysS strains respectively.Inducing with IPTG,theyall express rabies virus glycoprotein which is about 60 000 dalton in molecular weight bySDS-pAGE.Rabies virus glycoprotein were identified with RVgp McAb by Western-blot.Thin-layer Chromatography-scanning indicated that the expressing quantity ofglycoprotein is about 10%~14%of the cell total protein.

将狂犬病病毒糖蛋白(RVgp)基因BglⅡ片段(1675bp)分别正向插入到原核高效表达载体pET-17b和pET-17b2(用SacⅠ-NdeⅠ缺失掉pET-17b60bp含起始密码子ATG小片段)的BamHⅠ切点,构建重组质粒pET-17bRVgp和pET-17b2RVgp。将其分别转化表达受体菌E.coliBL21(DE3)和E,coliBL21(DE3)plysS.IPTG诱导表达,菌体经超声波裂解处理后SDS-pAGE,染色,在分子量约60000处可见重组质粒表达的较宽的蛋白带,以抗RVgpMcAb进行Western-blot检测,表明该表达蛋白为RVgp。通过扫描显示,表达的RVgp占菌体总蛋白的10%~14%,其中pET-17b2RVgp在E。coliBL21(DE3)中的表达量最高。

Expressions of the transgene in 3 lines of transgenic mice carrying rabies virus glycoprotein gene were monitored by assaying samples from liver, kidney, brain, spleen, muscles and sera using ELISA. The expressions were shown to be positive in liver and kidney, but negative in brain, spleen, muscles and sera. The glycoproteins were demonstrated to be distributed within cell plasma and on cell membrane by immunohistochemistry assay. One line of transgenic mice, TgN (oMT Rgp) 2Lge, highly expressed the...

Expressions of the transgene in 3 lines of transgenic mice carrying rabies virus glycoprotein gene were monitored by assaying samples from liver, kidney, brain, spleen, muscles and sera using ELISA. The expressions were shown to be positive in liver and kidney, but negative in brain, spleen, muscles and sera. The glycoproteins were demonstrated to be distributed within cell plasma and on cell membrane by immunohistochemistry assay. One line of transgenic mice, TgN (oMT Rgp) 2Lge, highly expressed the glycoprotein were inoculated with inactivated rabies virus. No one produced significant antibody response against the virus, indicating that the transgenic mice have developed immune tolerance to rabies virus.

对带有绵羊MT启动子-狂犬病病毒糖蛋白基因的(MT-Rgp)的TgN(oMT-Rgp)2Lge、TgN(oMT-Rgp)4Lge和TgN(oMT-Rgp)6Lge3株转基因小鼠系(子代)进行了表达检测。ELISA结果表明,小鼠肝脏、肾脏中均有糖蛋白表达产物,以肝脏的表达量较高;对肝脏的免疫组化检测结果显示,糖蛋白分布在肝脏实质细胞,主要位于细胞膜上和胞浆内。对8只TgN(oMT-Rgp)2Lge子代鼠一次接种灭活的狂犬病病毒8202株,接种前和接种后3周的血清抗体水平变化在转基因鼠和非转基因鼠之间有明显区别,非转基因鼠抗狂犬病病毒抗体水平明显升高,而转基因鼠抗体水平基本保持不变。经统计学分析,转基因鼠的抗体水平变化相差不显著,表明转基因小鼠对该病毒形成了部分免疫耐受性。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关virus glycoprotein的内容
在知识搜索中查有关virus glycoprotein的内容
在数字搜索中查有关virus glycoprotein的内容
在概念知识元中查有关virus glycoprotein的内容
在学术趋势中查有关virus glycoprotein的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社